FastQCFastQC Report
Fri 10 Feb 2017
SRR2031540_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031540_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352511
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29810.22040486177191906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26340.19474887819766346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.056.521741
GTATCAA38750.039.5935481
TCAACGC44100.033.282314
TATCAAC44800.033.1863862
ATCAACG44850.032.831663
AACGCAG45900.032.1840976
CAACGCA46200.031.8722955
ACGCAGA52500.027.9571447
CGCAGAG53950.027.293798
GTACATG45350.026.813671
TACATGG45400.026.2610132
ACATGGG48100.024.0956363
GCAGAGT59350.024.0101099
TAGCGCG601.7116507E-423.7528-29
CTATTCG1000.001653796223.754
GAGTACT31900.023.45219412-13
CAGAGTA55550.022.4887510-11
GTAATAC2554.9112714E-1022.3529423
TATACCG1302.9418807E-421.9230775
GGACCGT2005.3875374E-721.3756
Produced by FastQC (version 0.11.5)