##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031540_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1352511 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13990718005251 34.0 31.0 34.0 30.0 34.0 2 32.30483670742789 34.0 31.0 34.0 30.0 34.0 3 32.408869133042174 34.0 31.0 34.0 30.0 34.0 4 35.60186201812776 37.0 35.0 37.0 35.0 37.0 5 35.569290009471274 37.0 35.0 37.0 33.0 37.0 6 35.6982767607805 37.0 37.0 37.0 35.0 37.0 7 35.73357702820901 37.0 37.0 37.0 35.0 37.0 8 35.792949558266066 37.0 37.0 37.0 35.0 37.0 9 37.62695682327168 39.0 39.0 39.0 35.0 39.0 10-11 37.62965033186421 39.0 39.0 39.0 35.0 39.0 12-13 37.60156627191942 39.0 39.0 39.0 35.0 39.0 14-15 39.13455639177796 41.0 40.0 41.0 36.0 41.0 16-17 39.14046835848285 41.0 40.0 41.0 36.0 41.0 18-19 39.08828763684731 41.0 40.0 41.0 36.0 41.0 20-21 39.04630757162049 41.0 40.0 41.0 36.0 41.0 22-23 38.9797535842592 41.0 39.0 41.0 36.0 41.0 24-25 38.924421317090946 41.0 39.0 41.0 36.0 41.0 26-27 38.820379649407656 41.0 39.0 41.0 35.0 41.0 28-29 38.717210063356234 41.0 39.0 41.0 35.0 41.0 30-31 38.55236260555367 40.5 39.0 41.0 35.0 41.0 32-33 38.44026776861704 40.0 38.0 41.0 34.5 41.0 34-35 38.30928399103593 40.0 38.0 41.0 34.0 41.0 36-37 38.16875278648381 40.0 38.0 41.0 34.0 41.0 38-39 38.02074179063978 40.0 38.0 41.0 33.0 41.0 40-41 37.87362394834497 40.0 38.0 41.0 33.0 41.0 42-43 37.71260270711292 40.0 38.0 41.0 33.0 41.0 44-45 37.88804083663645 40.0 38.0 41.0 33.0 41.0 46-47 37.75111219058477 40.0 38.0 41.0 33.0 41.0 48-49 37.59706501462834 40.0 38.0 41.0 32.0 41.0 50-51 36.51759505098295 39.0 36.0 40.0 30.5 40.5 52-53 36.5602102311922 39.0 36.5 40.0 30.5 40.5 54-55 37.289714464429494 40.0 37.0 41.0 32.0 41.0 56-57 37.2733560022802 40.0 36.5 41.0 32.0 41.0 58-59 37.041275080202674 40.0 36.0 41.0 31.0 41.0 60-61 36.832028722871755 39.0 35.5 41.0 31.0 41.0 62-63 36.54179966003973 39.0 35.0 41.0 31.0 41.0 64-65 36.22425436835634 38.5 35.0 41.0 30.5 41.0 66-67 35.892308454422924 37.5 35.0 40.5 30.0 41.0 68-69 35.559626502113474 37.0 35.0 40.0 30.0 41.0 70-71 35.17846804942806 36.5 35.0 39.0 29.5 41.0 72-73 34.803011583639616 36.0 35.0 39.0 29.0 41.0 74-75 34.42206939536906 35.5 35.0 38.5 29.0 40.5 76-77 34.06603051657251 35.0 34.5 37.0 29.0 39.0 78-79 33.68967498231068 35.0 34.0 37.0 28.0 39.0 80-81 33.37309308390098 35.0 34.0 36.0 27.0 39.0 82-83 33.10880355132046 35.0 34.0 36.0 27.0 37.0 84-85 32.854393051147085 35.0 34.0 35.5 26.0 37.0 86-87 32.61784451290969 35.0 34.0 35.0 26.0 36.5 88-89 32.424927043107225 35.0 34.0 35.0 25.0 36.0 90-91 32.253175759753525 35.0 34.0 35.0 25.0 36.0 92-93 32.107405041437744 35.0 33.0 35.0 25.0 36.0 94-95 31.97520389852652 35.0 33.0 35.0 24.0 35.5 96-97 31.84277650976591 35.0 33.0 35.0 23.5 35.0 98-99 31.697386564693375 35.0 33.0 35.0 23.0 35.0 100-101 30.068419036887686 33.5 29.5 35.0 16.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2977290403418422 1101 2 0.24230872228805111 1101 3 0.3871696537364997 1101 4 0.33501365804725225 1101 5 0.08725631549514645 1101 6 0.15047707550193223 1101 7 0.23962893091497506 1101 8 0.29604965456832844 1101 9 0.4951410702675645 1101 10-11 0.5006703430932262 1101 12-13 0.5734368226455899 1101 14-15 0.7324965934021321 1101 16-17 0.6066602993268475 1101 18-19 0.7261409859341015 1101 20-21 0.7950190456251107 1101 22-23 0.6778990697037344 1101 24-25 0.6111385411922612 1101 26-27 0.6854505987480266 1101 28-29 0.7335419694440333 1101 30-31 0.8153223115931141 1101 32-33 0.7788215114788031 1101 34-35 0.7111681617290131 1101 36-37 0.7796625574761578 1101 38-39 0.7955908316133318 1101 40-41 0.673901243164444 1101 42-43 0.6096367304623627 1101 44-45 0.6187400372419987 1101 46-47 0.6559985673053959 1101 48-49 0.6384715824800367 1101 50-51 0.5885001680850905 1101 52-53 0.7555295831267799 1101 54-55 0.7338198820837931 1101 56-57 0.5077229952902726 1101 58-59 0.637494166556472 1101 60-61 0.6191241486839729 1101 62-63 0.5793209835905699 1101 64-65 0.6818707805367481 1101 66-67 0.8294851406571055 1101 68-69 0.6052985213489279 1101 70-71 0.42391179612426555 1101 72-73 0.49326805532635376 1101 74-75 0.6298971136401903 1101 76-77 0.5087043467007817 1101 78-79 0.5364045121081062 1101 80-81 0.5950071119984841 1101 82-83 0.601593701462285 1101 84-85 0.5249637498697126 1101 86-87 0.49910821474951916 1101 88-89 0.5627533032142367 1101 90-91 0.6089448919489371 1101 92-93 0.6388362596637975 1101 94-95 0.7478659376659671 1101 96-97 0.8448456899629058 1101 98-99 0.8268015689943411 1101 100-101 0.7625983180395934 1102 1 0.10533646343313308 1102 2 0.13884876463454532 1102 3 0.2438890459397598 1102 4 -0.001999668643492214 1102 5 0.10140502641804972 1102 6 0.112091224212854 1102 7 0.08750039685095601 1102 8 0.26383642751638803 1102 9 0.39343475565802066 1102 10-11 0.48703726300853134 1102 12-13 0.6048454663122982 1102 14-15 0.5767636254110968 1102 16-17 0.593525413288738 1102 18-19 0.5326424182419842 1102 20-21 0.5509562731752453 1102 22-23 0.5955351389527053 1102 24-25 0.46679927104653984 1102 26-27 0.5774346565385358 1102 28-29 0.518005788101398 1102 30-31 0.5383737501284216 1102 32-33 0.5391428466388462 1102 34-35 0.6402950766623761 1102 36-37 0.49650898916130615 1102 38-39 0.4948435405434637 1102 40-41 0.5024278342716784 1102 42-43 0.5494947451590875 1102 44-45 0.4982642619275168 1102 46-47 0.6250482621615419 1102 48-49 0.5971538592268217 1102 50-51 0.5666070307080773 1102 52-53 0.6042853434954409 1102 54-55 0.5764080001557801 1102 56-57 0.5720099550610982 1102 58-59 0.6686288034975618 1102 60-61 0.5822129517727745 1102 62-63 0.5180182061587644 1102 64-65 0.4910201142022075 1102 66-67 0.5248818276666967 1102 68-69 0.45926788241506955 1102 70-71 0.4199885180074361 1102 72-73 0.5191392721653116 1102 74-75 0.4757658395089166 1102 76-77 0.5082709178802602 1102 78-79 0.614643396153447 1102 80-81 0.4677558976504983 1102 82-83 0.4586693024088575 1102 84-85 0.5855366730232845 1102 86-87 0.5315207194200866 1102 88-89 0.5559130989546759 1102 90-91 0.5170081625928518 1102 92-93 0.5056640090721842 1102 94-95 0.3788162428098225 1102 96-97 0.35784606360844506 1102 98-99 0.22601691337097662 1102 100-101 0.286681018251322 1103 1 0.05696792896777225 1103 2 0.20723795975669645 1103 3 0.12334137712363002 1103 4 0.07377732539235637 1103 5 -0.05191157393544188 1103 6 -0.03107260026840919 1103 7 0.004066853951847804 1103 8 0.06576045117930107 1103 9 0.1871986269485859 1103 10-11 0.10779960896154961 1103 12-13 0.16346806229153543 1103 14-15 0.18889409423044867 1103 16-17 0.1933062973388715 1103 18-19 0.20417146825530352 1103 20-21 0.1799913559389239 1103 22-23 0.17914865554175918 1103 24-25 0.18563799686509697 1103 26-27 0.27657191270646564 1103 28-29 0.249066180169649 1103 30-31 0.38406325919570605 1103 32-33 0.15210498759940805 1103 34-35 0.1645136438135566 1103 36-37 0.20922094248113154 1103 38-39 0.1964120556394633 1103 40-41 0.44455822238534637 1103 42-43 0.2881386474406398 1103 44-45 0.16896486802725263 1103 46-47 0.2981910224575728 1103 48-49 0.30198651597745396 1103 50-51 0.46087248786546553 1103 52-53 0.4581390402195993 1103 54-55 0.4821050148522872 1103 56-57 0.3854377678561818 1103 58-59 0.44446108093143977 1103 60-61 0.44468480566319357 1103 62-63 0.41986152413111455 1103 64-65 0.4360611530458627 1103 66-67 0.3946349489592791 1103 68-69 0.5538198447739333 1103 70-71 0.5183501924891019 1103 72-73 0.5008985755137942 1103 74-75 0.38257094333678054 1103 76-77 0.35704580549758447 1103 78-79 0.3619542825440405 1103 80-81 0.34026673238945193 1103 82-83 0.2107753174039786 1103 84-85 0.24706911267560372 1103 86-87 0.2504510461974263 1103 88-89 0.2507705323523055 1103 90-91 0.048743254234160815 1103 92-93 0.11239284602248034 1103 94-95 0.18325666990433476 1103 96-97 0.14950513048924918 1103 98-99 0.21912182269813485 1103 100-101 0.07015168576809216 1104 1 -0.061864314480871485 1104 2 -0.07199283531625866 1104 3 0.05679073436674997 1104 4 -0.07783398525910457 1104 5 -0.16646412721612336 1104 6 -0.19064953901917647 1104 7 -0.2107166273204868 1104 8 -8.887851224059773E-4 1104 9 0.1415220634375487 1104 10-11 0.22041607715087963 1104 12-13 0.23903563246458503 1104 14-15 0.38627654516373156 1104 16-17 0.2994466302783181 1104 18-19 0.3527194409529173 1104 20-21 0.40434754009122287 1104 22-23 0.3211175175093359 1104 24-25 0.3786655344073253 1104 26-27 0.27162105611114384 1104 28-29 0.3801758760779421 1104 30-31 0.4525145664000405 1104 32-33 0.4916078424302768 1104 34-35 0.38701334803291587 1104 36-37 0.48844735561876007 1104 38-39 0.43969966104327085 1104 40-41 0.30253276041981536 1104 42-43 0.431532003550295 1104 44-45 0.6449533230883162 1104 46-47 0.6800591583384659 1104 48-49 0.6541495122864021 1104 50-51 0.5695280516817789 1104 52-53 0.6419849626096834 1104 54-55 0.7212053158912468 1104 56-57 0.6653119770994351 1104 58-59 0.6828080542556805 1104 60-61 0.682618051260448 1104 62-63 0.6654828752570481 1104 64-65 0.5531333191387375 1104 66-67 0.6258127109296296 1104 68-69 0.6810832969502201 1104 70-71 0.502272141457091 1104 72-73 0.6220068692027851 1104 74-75 0.6422736436313983 1104 76-77 0.6572299198135099 1104 78-79 0.6245333582186063 1104 80-81 0.756592216702856 1104 82-83 0.6082190423995542 1104 84-85 0.6305242394669079 1104 86-87 0.548649048327043 1104 88-89 0.6914880596554127 1104 90-91 0.5566102887886402 1104 92-93 0.7048038452644008 1104 94-95 0.6109613099356501 1104 96-97 0.47223247624877374 1104 98-99 0.5131325204629107 1104 100-101 0.4250855258479014 1105 1 0.31628700911257823 1105 2 0.31622211194612504 1105 3 0.3429621074012914 1105 4 0.3287439185860208 1105 5 0.2826294300171952 1105 6 0.18185673666882707 1105 7 0.1827208935814042 1105 8 0.22201860223940173 1105 9 0.3557417691661371 1105 10-11 0.32038857196038606 1105 12-13 0.28792525543189385 1105 14-15 0.39368383629150117 1105 16-17 0.3653133713841754 1105 18-19 0.3647971543775057 1105 20-21 0.4165283126662942 1105 22-23 0.40989137297263056 1105 24-25 0.4450373336016966 1105 26-27 0.3333326706726538 1105 28-29 0.38497426917420796 1105 30-31 0.4609913521557658 1105 32-33 0.30133710399439906 1105 34-35 0.36196349060944755 1105 36-37 0.3524488626028912 1105 38-39 0.39815419694266296 1105 40-41 0.23901249189271567 1105 42-43 0.3614551937701904 1105 44-45 0.4462757562670845 1105 46-47 0.5318932017106732 1105 48-49 0.3737982512109568 1105 50-51 0.3983382713274537 1105 52-53 0.3582955405735859 1105 54-55 0.3732598785450634 1105 56-57 0.3998882538385047 1105 58-59 0.5521396303508723 1105 60-61 0.3456101928295965 1105 62-63 0.4010399202031252 1105 64-65 0.45191899569135074 1105 66-67 0.4439726887616686 1105 68-69 0.31396442129142343 1105 70-71 0.4422999060017361 1105 72-73 0.3707223836317439 1105 74-75 0.2902386007901825 1105 76-77 0.25098815260161444 1105 78-79 0.32729848300208175 1105 80-81 0.4516378016079017 1105 82-83 0.51026712119954 1105 84-85 0.3696897197020661 1105 86-87 0.42309945640032964 1105 88-89 0.44082602405291027 1105 90-91 0.357205165315591 1105 92-93 0.4289800204458558 1105 94-95 0.306995853178865 1105 96-97 0.2881548419954605 1105 98-99 0.35655120406312335 1105 100-101 0.2603813056098119 1106 1 0.15761735136580057 1106 2 0.16633208318838655 1106 3 0.22958588700446825 1106 4 0.2940229512521313 1106 5 0.17058396491111694 1106 6 0.07323690157406304 1106 7 0.0466508424163834 1106 8 -0.05358714311263668 1106 9 0.029794493406789968 1106 10-11 0.12341226482568146 1106 12-13 0.17415342252622423 1106 14-15 0.19081529064827407 1106 16-17 0.17915283918531344 1106 18-19 0.20151825594859218 1106 20-21 0.18798690307246346 1106 22-23 0.1799069135096829 1106 24-25 0.1683493008478152 1106 26-27 0.24814690704369724 1106 28-29 0.24105198734837074 1106 30-31 0.15643877505973336 1106 32-33 0.15798597562748995 1106 34-35 0.19195595267454024 1106 36-37 0.28176716997509743 1106 38-39 0.3124869864432398 1106 40-41 0.18946864021449272 1106 42-43 0.1725402175456665 1106 44-45 0.24167890671083825 1106 46-47 0.24813770960227544 1106 48-49 0.17286581898783027 1106 50-51 0.20313867094126437 1106 52-53 0.1609081320226764 1106 54-55 0.14938514766483024 1106 56-57 0.18065632293969003 1106 58-59 0.3200080211050249 1106 60-61 0.35732561513465555 1106 62-63 0.35558707618158536 1106 64-65 0.33937014109604746 1106 66-67 0.30158122728000336 1106 68-69 0.19854560123638265 1106 70-71 0.32651645125258 1106 72-73 0.22121118773321058 1106 74-75 0.297194410937486 1106 76-77 0.2228590060920368 1106 78-79 0.16778016220615655 1106 80-81 0.2183722752690187 1106 82-83 0.24024448157994271 1106 84-85 0.23800109320674068 1106 86-87 0.20814052781968684 1106 88-89 0.25148252139139515 1106 90-91 0.08064878827428146 1106 92-93 0.06429111515019059 1106 94-95 -0.033232195167183676 1106 96-97 0.06976512043438987 1106 98-99 0.1459975788843586 1106 100-101 0.21703688910277208 1107 1 0.19844613514562326 1107 2 0.12642134884649892 1107 3 0.17777713428206 1107 4 0.1704818197073621 1107 5 0.13985860819031615 1107 6 0.14317224745406776 1107 7 0.0513509625441273 1107 8 0.10728637116108075 1107 9 0.1549226785011868 1107 10-11 0.15832744291750345 1107 12-13 0.1384874460243921 1107 14-15 0.23004115034297 1107 16-17 0.13263385802486738 1107 18-19 0.17938130055212298 1107 20-21 0.19539600152837977 1107 22-23 0.1671531070227843 1107 24-25 0.266887014123391 1107 26-27 0.35289976618171437 1107 28-29 0.20085558336755582 1107 30-31 0.1350880215755339 1107 32-33 0.23075482249880253 1107 34-35 0.2599331186363898 1107 36-37 0.2500846978828264 1107 38-39 0.17144431667734494 1107 40-41 0.3592589538162727 1107 42-43 0.3597710288474403 1107 44-45 0.2999738252095341 1107 46-47 0.4523377857808413 1107 48-49 0.34057098838748345 1107 50-51 0.33817007018533474 1107 52-53 0.29839336021656493 1107 54-55 0.15285469088640724 1107 56-57 0.1712231208027859 1107 58-59 0.23236237842158403 1107 60-61 0.220658329924035 1107 62-63 0.26428876871315765 1107 64-65 0.26323551988910054 1107 66-67 0.32404291824894216 1107 68-69 0.2223037928743281 1107 70-71 0.28915110664221544 1107 72-73 0.3137730339892286 1107 74-75 0.367627847469997 1107 76-77 0.3012179438893057 1107 78-79 0.2845799785962697 1107 80-81 0.46845284875691817 1107 82-83 0.283500236480279 1107 84-85 0.41150755373550396 1107 86-87 0.3684024160715822 1107 88-89 0.49459959222770067 1107 90-91 0.40875745993487556 1107 92-93 0.3924376319338947 1107 94-95 0.4419291959707188 1107 96-97 0.32819603721418034 1107 98-99 0.440298347506598 1107 100-101 0.5117063424726922 1108 1 0.2291686678888496 1108 2 0.2027408322503632 1108 3 0.13601783077259455 1108 4 0.09436146222726904 1108 5 0.20128578120245777 1108 6 0.29493159713766204 1108 7 0.2960386292038706 1108 8 0.1960694058632484 1108 9 0.3283151839785887 1108 10-11 0.24984340877635702 1108 12-13 0.25241056148509955 1108 14-15 0.34456020063352355 1108 16-17 0.30729153458895553 1108 18-19 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-0.7521821576223289 2316 62-63 -0.6586857731661837 2316 64-65 -0.8667553455893753 2316 66-67 -0.6468887332167697 2316 68-69 -0.6888568840564773 2316 70-71 -0.9865048705423973 2316 72-73 -0.6486350978267978 2316 74-75 -0.4269046881616134 2316 76-77 -0.3868024100559708 2316 78-79 -0.511557199603601 2316 80-81 -0.4985739690825923 2316 82-83 -0.5465819742133888 2316 84-85 -0.48688309697713095 2316 86-87 -0.4290674609261629 2316 88-89 -0.4402759760650454 2316 90-91 -0.38426456751052385 2316 92-93 -0.5064114880839554 2316 94-95 -0.3568637920637592 2316 96-97 -0.34433223796501977 2316 98-99 -0.3050114940687223 2316 100-101 -0.09391069096941607 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 59.0 7 1214.0 8 2523.0 9 1970.0 10 1927.0 11 2226.0 12 2609.0 13 2611.0 14 2539.0 15 2479.0 16 2689.0 17 3019.0 18 3666.0 19 4530.0 20 5885.0 21 7282.0 22 8214.0 23 8577.0 24 8307.0 25 8003.0 26 8326.0 27 8911.0 28 10032.0 29 11966.0 30 14336.0 31 18249.0 32 23453.0 33 31874.0 34 47128.0 35 78839.0 36 156041.0 37 328549.0 38 417275.0 39 117112.0 40 89.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95439297721054 18.729755247831626 13.462515277140074 23.85333649781776 2 15.781091614042325 20.986816373397332 39.05639214764242 24.175699864917917 3 17.99800519182469 25.993060315221094 30.697717061081203 25.31121743187301 4 13.268136081702847 17.57530992354221 37.50549903106148 31.651054963693454 5 14.069904052536355 37.347126936490724 34.400385653055686 14.182583357917236 6 30.66991691749642 36.73729825487556 18.334564376925584 14.258220450702433 7 26.91135229214402 32.88609112975791 22.765360133854735 17.437196444243337 8 26.001119399398597 34.23550714190125 20.957833244979156 18.805540213721 9 27.01582464024322 15.515067899632609 20.058173279182203 37.410934180941965 10-11 24.637951188567044 25.650586205953225 28.870670922454604 20.840791683025127 12-13 25.684707924741463 23.84989844814571 28.3563682661361 22.10902536097673 14-15 23.238701940316936 25.01162652281571 25.943264047390375 25.806407489476978 16-17 22.692495661772806 28.00284064233119 26.477012016907807 22.827651678988193 18-19 22.79341905537182 27.07478904053276 28.20524195366988 21.926549950425542 20-21 23.683763015605788 26.38858390061153 27.80121566478942 22.126437418993262 22-23 23.68675744596532 26.190286067913682 27.423473820175953 22.699482665945045 24-25 23.216188260206387 26.41778883868597 27.480331028731005 22.88569187237664 26-27 23.122621553540046 26.569987231157455 27.47038656247528 22.837004652827222 28-29 23.198480455981503 26.557935573167242 27.695227617372424 22.548356353478827 30-31 23.309533157216467 26.569173929084496 27.42136662844147 22.69992628525757 32-33 23.241659402400426 26.64858178602614 27.283068307762377 22.826690503811058 34-35 23.38790590242889 26.608545143070927 27.212532837071198 22.79101611742899 36-37 23.421768843284823 26.50865686120113 27.308354608576195 22.76121968693785 38-39 23.307130219273635 26.505551526013466 27.326247254181297 22.861071000531606 40-41 23.1961884228668 26.493832582507647 27.397078471080828 22.912900523544725 42-43 23.224173407831803 26.36492420394363 27.410534923560697 23.000367464663874 44-45 23.160440099932643 26.538120577207874 27.28702391329904 23.014415409560442 46-47 23.24477333078006 26.620902388558164 27.028329909453568 23.105994371208208 48-49 23.186167337888325 26.63793734459438 26.923887382634792 23.252007934882503 50-51 23.09020037544981 26.966471991725022 26.83678727936409 23.10654035346108 52-53 23.113009801768712 26.858635530505854 26.844809395265546 23.18354527245989 54-55 22.933270043644747 26.744625367187403 26.947359393010483 23.37474519615737 56-57 22.987280694944438 26.802148004711235 26.845216046302028 23.365355254042296 58-59 23.102178096887936 26.544442152411328 26.95889349513608 23.39448625556465 60-61 23.067945473271568 26.446808935380194 27.04377265693218 23.441472934416062 62-63 22.933122170540575 26.564294116646742 26.953939746146244 23.548643966666443 64-65 22.916523414597 26.437714739473467 26.968948866219943 23.67681297970959 66-67 22.94975789476019 26.474313332756626 26.898302490700633 23.67762628178255 68-69 22.977373196964756 26.575569440840034 26.817268029613068 23.62978933258214 70-71 23.055043544932353 26.396569048236945 26.682370790329983 23.866016616500715 72-73 23.02997905377479 26.51727046951929 26.664071493688407 23.788678983017515 74-75 23.0544890207917 26.693461273143065 26.61845264105061 23.633597065014627 76-77 23.126835937009016 26.620596801061136 26.553647253146185 23.69892000878366 78-79 23.251936583140544 26.572057454615894 26.525144712316575 23.650861249926987 80-81 23.19877620218985 26.731908280228406 26.446845903656236 23.622469613925507 82-83 23.188425084897645 26.485773498330147 26.489913945247025 23.835887471525186 84-85 23.212565369154113 26.481226400376777 26.443444822260226 23.862763408208878 86-87 23.33319285388437 26.492353851465904 26.48481232315301 23.68964097149672 88-89 23.299921405445133 26.309508758154276 26.437418993265123 23.95315084313547 90-91 23.44280379235363 26.282447980090367 26.423555889748773 23.851192337807234 92-93 23.469347014552934 26.221672134274694 26.472464918954447 23.836515932217928 94-95 23.60002987036704 25.994612982814928 26.51423907088371 23.89111807593432 96-97 23.569678915735253 25.901083244424626 26.50610605015412 24.023131789685998 98-99 23.68941916184046 25.90540853272173 26.37357478053783 24.031597524899983 100-101 23.997180059903396 25.89731248026818 26.3679556025792 23.73755185724922 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 117.0 1 86.0 2 78.0 3 114.5 4 107.0 5 118.0 6 186.0 7 332.0 8 621.5 9 844.0 10 752.5 11 622.5 12 644.0 13 702.0 14 767.5 15 830.0 16 921.0 17 1035.5 18 1135.5 19 1228.5 20 1385.5 21 1580.5 22 1835.5 23 2173.0 24 2577.5 25 3282.0 26 4310.5 27 5747.5 28 7562.0 29 10053.5 30 12934.0 31 15575.0 32 18237.5 33 20555.5 34 22331.0 35 23959.5 36 26408.5 37 28920.0 38 30918.5 39 33138.5 40 35670.5 41 37815.5 42 39970.5 43 42879.0 44 45722.5 45 49185.5 46 53450.5 47 56748.0 48 58825.0 49 60198.0 50 59865.0 51 58506.0 52 56686.5 53 54567.0 54 51888.5 55 48343.5 56 43749.0 57 38872.5 58 34873.5 59 30454.5 60 25337.0 61 20408.0 62 16380.5 63 12632.0 64 9085.0 65 6624.0 66 4965.0 67 3663.0 68 2689.0 69 1983.5 70 1490.5 71 1087.5 72 778.5 73 579.5 74 457.5 75 352.0 76 249.0 77 174.0 78 115.5 79 84.5 80 75.0 81 77.5 82 63.0 83 38.5 84 29.0 85 29.5 86 25.5 87 22.5 88 17.0 89 14.0 90 10.5 91 6.0 92 4.0 93 3.0 94 2.5 95 4.5 96 5.5 97 3.0 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 3.696827604359595E-5 48-49 1.478731041743838E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1352511.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.65768573958435 #Duplication Level Percentage of deduplicated Percentage of total 1 64.62321233575395 30.797927449797925 2 15.851608463024732 15.109019491955367 3 7.285305378600682 10.416023827507646 4 3.9341515987840454 7.499702421869333 5 2.3751571054635248 5.659724545716073 6 1.499877266927001 4.288840766109221 7 1.0560162851420287 3.5229104577227464 8 0.7112190488294821 2.711604313689724 9 0.5228793723142241 2.242729872491617 >10 2.099219105520726 15.739700863932693 >50 0.03461416700401691 1.079654129984732 >100 0.00626965703230336 0.41689534371040515 >500 0.0 0.0 >1k 4.70215603331659E-4 0.5152665155125167 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2981 0.22040486177191906 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2634 0.19474887819766346 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.39365520871919E-5 0.0 3 0.0 0.0 0.0 7.39365520871919E-5 0.0 4 0.0 0.0 0.0 7.39365520871919E-5 0.0 5 0.0 0.0 0.0 7.39365520871919E-5 0.0 6 0.0 0.0 0.0 7.39365520871919E-5 0.0 7 0.0 0.0 0.0 7.39365520871919E-5 0.0 8 0.0 0.0 0.0 7.39365520871919E-5 0.0 9 0.0 0.0 0.0 7.39365520871919E-5 0.0 10-11 0.0 0.0 0.0 7.39365520871919E-5 0.0 12-13 0.0 0.0 0.0 7.39365520871919E-5 0.0 14-15 0.0 0.0 0.0 1.8484138021797976E-4 0.0 16-17 0.0 0.0 0.0 2.957462083487676E-4 0.0 18-19 0.0 0.0 0.0 4.805875885667473E-4 0.0 20-21 0.0 0.0 0.0 6.654289687847272E-4 0.0 22-23 0.0 0.0 0.0 0.001515699317787434 0.0 24-25 0.0 0.0 0.0 0.0034380496720544233 7.39365520871919E-5 26-27 0.0 0.0 0.0 0.006395511755542099 7.39365520871919E-5 28-29 0.0 0.0 0.0 0.018447169745754378 7.39365520871919E-5 30-31 0.0 0.0 0.0 0.04813269540876193 7.39365520871919E-5 32-33 0.0 0.0 0.0 0.11016546260991593 7.39365520871919E-5 34-35 0.0 0.0 0.0 0.20413882031273684 1.478731041743838E-4 36-37 0.0 0.0 0.0 0.36273272453976346 1.478731041743838E-4 38-39 0.0 0.0 0.0 0.5730082786757371 1.478731041743838E-4 40-41 0.0 0.0 0.0 0.7812875459053568 1.478731041743838E-4 42-43 0.0 0.0 0.0 0.9782175523895924 2.2180965626157568E-4 44-45 0.0 0.0 0.0 1.2094171507662415 2.957462083487676E-4 46-47 0.0 0.0 0.0 1.5017622777189983 2.957462083487676E-4 48-49 0.0 0.0 0.0 1.7961776281301964 2.957462083487676E-4 50-51 0.0 0.0 0.0 2.0732178888009045 2.957462083487676E-4 52-53 0.0 0.0 0.0 2.327781437637106 4.0665103647955544E-4 54-55 0.0 0.0 0.0 2.61084752730292 5.545241406539393E-4 56-57 0.0 0.0 0.0 2.9475915537840356 6.284606927411311E-4 58-59 0.0 0.0 0.0 3.305518402438132 6.65428968784727E-4 60-61 0.0 0.0 0.0 3.659193899347214 6.65428968784727E-4 62-63 0.0 0.0 0.0 3.9984517685992937 6.65428968784727E-4 64-65 0.0 0.0 0.0 4.346471119273707 6.65428968784727E-4 66-67 0.0 0.0 0.0 4.752197948852173 6.65428968784727E-4 68-69 0.0 0.0 0.0 5.212674795251203 6.65428968784727E-4 70-71 0.0 0.0 0.0 5.6654622402331665 7.39365520871919E-4 72-73 0.0 0.0 0.0 6.136438077028579 8.872386250463027E-4 74-75 0.0 0.0 0.0 6.591813301333593 8.872386250463027E-4 76-77 0.0 0.0 0.0 7.075765003020308 8.872386250463027E-4 78-79 0.0 0.0 0.0 7.594060233151524 8.872386250463027E-4 80-81 0.0 0.0 0.0 8.124481057825038 9.611751771334946E-4 82-83 0.0 0.0 0.0 8.665400872894935 0.0010351117292206865 84-85 0.0 0.0 0.0 9.238113405362322 0.0010351117292206865 86-87 0.0 0.0 0.0 9.847424531112871 0.0010351117292206865 88-89 0.0 0.0 0.0 10.541688755211602 0.0010351117292206865 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2185 0.0 56.52174 1 GTATCAA 3875 0.0 39.593548 1 TCAACGC 4410 0.0 33.28231 4 TATCAAC 4480 0.0 33.186386 2 ATCAACG 4485 0.0 32.83166 3 AACGCAG 4590 0.0 32.184097 6 CAACGCA 4620 0.0 31.872295 5 ACGCAGA 5250 0.0 27.957144 7 CGCAGAG 5395 0.0 27.29379 8 GTACATG 4535 0.0 26.81367 1 TACATGG 4540 0.0 26.261013 2 ACATGGG 4810 0.0 24.095636 3 GCAGAGT 5935 0.0 24.010109 9 TAGCGCG 60 1.7116507E-4 23.75 28-29 CTATTCG 100 0.0016537962 23.75 4 GAGTACT 3190 0.0 23.452194 12-13 CAGAGTA 5555 0.0 22.48875 10-11 GTAATAC 255 4.9112714E-10 22.352942 3 TATACCG 130 2.9418807E-4 21.923077 5 GGACCGT 200 5.3875374E-7 21.375 6 >>END_MODULE