FastQCFastQC Report
Fri 10 Feb 2017
SRR2031540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1352511
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT100110.740178822944878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36890.2727519406496509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32110.2374102687519732No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT29000.2144160010528565RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT20280.14994332763282517RNA PCR Primer, Index 31 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16180.11962934127707647No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.058.338921
GTATCAA39450.041.1133081
TCAACGC46400.034.6002584
AACGCAG47000.033.754316
TATCAAC48250.033.5689432
CAACGCA47600.033.5284085
ATCAACG48450.033.23433
ACGCAGA52800.029.956497
CGCAGAG53500.029.5645358
GTACATG42850.028.305081
TACATGG43450.026.7828562
GCAGAGT60800.025.546119
ACATGGG44500.025.4037323
CATGGGG30750.023.9423714
GAGTACT35200.023.54687712-13
CAGAGTA56650.023.35102310-11
GTATAGA4350.021.8683381
GTACTTT38150.021.16581514-15
AGAGTAC53950.020.90993910-11
GTGTACG2451.7319871E-719.413731
Produced by FastQC (version 0.11.5)