FastQCFastQC Report
Fri 10 Feb 2017
SRR2031539_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031539_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789703
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29200.1631555626827468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22930.1281218168601159No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.049.4190941
GTATCAA34800.043.4050831
CAACGCA44700.033.4730875
TCAACGC44450.033.4476244
ATCAACG45250.032.856293
AACGCAG45500.032.571366
TATCAAC47500.031.2999342
ACGCAGA51350.028.9532017
CGCAGAG51650.028.325218
TAGGACG908.949613E-426.3888344
GTACATG46800.026.287341
TACATGG45800.025.720472
ACATGGG48400.024.1425113
GCAGAGT61200.023.4394939
AGAGTAC53450.022.30584710-11
TCGCCGG2250.020.05551394-95
GAGTACT33450.019.66737412-13
TATACCG1456.147959E-419.6551325
CATGGGG31000.019.6128624
CAGAGTA58350.019.33372110-11
Produced by FastQC (version 0.11.5)