##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031539_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1789703 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.251327734266525 34.0 31.0 34.0 30.0 34.0 2 32.4204490912738 34.0 31.0 34.0 31.0 34.0 3 32.52654714217946 34.0 31.0 34.0 31.0 34.0 4 35.726252903414704 37.0 35.0 37.0 35.0 37.0 5 35.699952450211015 37.0 37.0 37.0 35.0 37.0 6 35.82441444194931 37.0 37.0 37.0 35.0 37.0 7 35.85384949346344 37.0 37.0 37.0 35.0 37.0 8 35.90652974264445 37.0 37.0 37.0 35.0 37.0 9 37.746119328179034 39.0 39.0 39.0 35.0 39.0 10-11 37.75163113656288 39.0 39.0 39.0 35.0 39.0 12-13 37.727968551206544 39.0 39.0 39.0 35.0 39.0 14-15 39.27116566268258 41.0 40.0 41.0 36.0 41.0 16-17 39.28184508826325 41.0 40.0 41.0 36.5 41.0 18-19 39.23429725490766 41.0 40.0 41.0 36.0 41.0 20-21 39.19218384279402 41.0 40.0 41.0 36.0 41.0 22-23 39.124755057124005 41.0 39.5 41.0 36.0 41.0 24-25 39.075835208411675 41.0 39.0 41.0 36.0 41.0 26-27 38.97968322118251 41.0 39.0 41.0 36.0 41.0 28-29 38.88109200241605 41.0 39.0 41.0 35.0 41.0 30-31 38.73375442741058 41.0 39.0 41.0 35.0 41.0 32-33 38.634677653219555 40.5 39.0 41.0 35.0 41.0 34-35 38.5130747950917 40.0 38.0 41.0 35.0 41.0 36-37 38.37559639783808 40.0 38.0 41.0 34.0 41.0 38-39 38.234391963359286 40.0 38.0 41.0 34.0 41.0 40-41 38.11089242181524 40.0 38.0 41.0 33.5 41.0 42-43 37.97193193507526 40.0 38.0 41.0 33.0 41.0 44-45 38.15367940937686 40.0 38.0 41.0 33.5 41.0 46-47 38.048964269490526 40.0 38.0 41.0 33.0 41.0 48-49 37.92976320652086 40.0 38.0 41.0 33.0 41.0 50-51 36.8850225428465 39.0 36.5 40.0 31.5 40.5 52-53 36.947373670379946 39.5 37.0 40.0 31.5 40.5 54-55 37.71571093080807 40.0 38.0 41.0 33.0 41.0 56-57 37.744455923692364 40.0 37.5 41.0 33.0 41.0 58-59 37.544097540206394 40.0 37.0 41.0 33.0 41.0 60-61 37.365514278067366 40.0 36.5 41.0 32.0 41.0 62-63 37.106609308918856 39.5 36.0 41.0 32.0 41.0 64-65 36.81951893694094 39.0 35.5 41.0 31.0 41.0 66-67 36.51507820012594 39.0 35.0 41.0 31.0 41.0 68-69 36.197885626833056 38.0 35.0 41.0 31.0 41.0 70-71 35.82411774467607 37.0 35.0 40.0 30.5 41.0 72-73 35.43067620716957 37.0 35.0 39.0 30.0 41.0 74-75 35.03176616455356 36.0 35.0 39.0 30.0 41.0 76-77 34.64679418875646 36.0 35.0 38.5 29.0 40.5 78-79 34.23851527320455 35.0 35.0 37.0 29.0 39.0 80-81 33.88694576697922 35.0 34.0 37.0 29.0 39.0 82-83 33.579056413270806 35.0 34.0 36.5 28.5 38.5 84-85 33.28275054576095 35.0 34.0 36.0 27.5 37.0 86-87 33.02517708245446 35.0 34.0 36.0 27.0 37.0 88-89 32.81123208711166 35.0 34.0 35.0 27.0 36.5 90-91 32.62272092073377 35.0 34.0 35.0 26.0 36.0 92-93 32.45785809153809 35.0 34.0 35.0 26.0 36.0 94-95 32.31363080913425 35.0 34.0 35.0 25.0 36.0 96-97 32.189414109491906 35.0 33.5 35.0 25.0 35.5 98-99 32.05009937403022 35.0 33.0 35.0 24.5 35.0 100-101 30.424726896026883 34.0 30.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11504167773009044 1101 2 0.1192831673304866 1101 3 0.0989947511846836 1101 4 -0.003026432720780292 1101 5 -0.06754599399544503 1101 6 0.0334518811696114 1101 7 0.016600598632145136 1101 8 0.04825503393482933 1101 9 0.12017701594494667 1101 10-11 0.24425864763326643 1101 12-13 0.29010665132056346 1101 14-15 0.34885266723320996 1101 16-17 0.25429574585952963 1101 18-19 0.34480722713400525 1101 20-21 0.3959487164012927 1101 22-23 0.35472958711662983 1101 24-25 0.33828462915282387 1101 26-27 0.3587292452873214 1101 28-29 0.329903410864155 1101 30-31 0.3977879227837704 1101 32-33 0.4522078700014802 1101 34-35 0.3588058589557761 1101 36-37 0.3966574211562133 1101 38-39 0.3983050248317568 1101 40-41 0.2735588295006721 1101 42-43 0.41041449492542625 1101 44-45 0.2638713421238279 1101 46-47 0.1818364148308831 1101 48-49 0.18064564226337154 1101 50-51 0.22620865483046515 1101 52-53 0.25286714601284643 1101 54-55 0.24089008428483538 1101 56-57 0.23250943991398998 1101 58-59 0.2598391192774585 1101 60-61 0.20919912090412396 1101 62-63 0.2764477365248439 1101 64-65 0.25618985561321495 1101 66-67 0.28888999078693445 1101 68-69 0.2185909293673447 1101 70-71 0.32353481263665884 1101 72-73 0.3440822074220833 1101 74-75 0.42280742692013007 1101 76-77 0.41762071939565004 1101 78-79 0.45661553319130377 1101 80-81 0.5104421059984929 1101 82-83 0.40824207422389946 1101 84-85 0.46941072431454955 1101 86-87 0.4705563063427931 1101 88-89 0.42803057402606015 1101 90-91 0.35332441220077726 1101 92-93 0.3584085554014038 1101 94-95 0.29551617381349615 1101 96-97 0.2925939669547333 1101 98-99 0.29408688541000316 1101 100-101 0.11643237755729174 1102 1 0.07571168863982791 1102 2 0.11820857169068688 1102 3 0.24390032782887516 1102 4 0.077917457431667 1102 5 0.0706034720087132 1102 6 0.12059292316813242 1102 7 0.23044565926966243 1102 8 0.2880996032380594 1102 9 0.3414570738484315 1102 10-11 0.4212832222724856 1102 12-13 0.3953023793538293 1102 14-15 0.5354191651027733 1102 16-17 0.4953358952674165 1102 18-19 0.5626838705283319 1102 20-21 0.5583973046076878 1102 22-23 0.6227910091859954 1102 24-25 0.6086452843708727 1102 26-27 0.6022297643582206 1102 28-29 0.5312426930406033 1102 30-31 0.5556179686680522 1102 32-33 0.5599477058535598 1102 34-35 0.5218844645173633 1102 36-37 0.5963512617789917 1102 38-39 0.578471761500353 1102 40-41 0.5244375518201849 1102 42-43 0.6114610897938846 1102 44-45 0.5331972895933177 1102 46-47 0.5809974483235081 1102 48-49 0.4866792801711384 1102 50-51 0.5049378424646918 1102 52-53 0.5385291661364207 1102 54-55 0.513843452434223 1102 56-57 0.3652568703149015 1102 58-59 0.46002244508227363 1102 60-61 0.4585195738826471 1102 62-63 0.4672537096705156 1102 64-65 0.44170368297525187 1102 66-67 0.4929398004609382 1102 68-69 0.40232750022382646 1102 70-71 0.4313264234999039 1102 72-73 0.4365698117976038 1102 74-75 0.4069769530995302 1102 76-77 0.2230148302522892 1102 78-79 0.3050740378972776 1102 80-81 0.23702223443222437 1102 82-83 0.20042906654482096 1102 84-85 0.2638747604936569 1102 86-87 0.28136301913148287 1102 88-89 0.2942410394464332 1102 90-91 0.22238223907675803 1102 92-93 0.248969918035165 1102 94-95 0.4559362910469176 1102 96-97 0.4046686392004304 1102 98-99 0.34106610408282023 1102 100-101 0.24455307673126825 1103 1 0.23208545509857004 1103 2 0.19150789684758251 1103 3 0.31041419180042595 1103 4 0.1876472435291774 1103 5 0.13533265157742136 1103 6 0.15264488459715153 1103 7 0.2100577787792517 1103 8 0.20334816299817504 1103 9 0.29772358047662095 1103 10-11 0.29222346960855106 1103 12-13 0.29275964035488045 1103 14-15 0.2534743570957403 1103 16-17 0.2490605141651514 1103 18-19 0.33462584806760987 1103 20-21 0.34050917405715353 1103 22-23 0.2781234614564312 1103 24-25 0.32536121820177044 1103 26-27 0.4116060612125736 1103 28-29 0.36946039218954496 1103 30-31 0.3679170959050282 1103 32-33 0.3033392021069119 1103 34-35 0.2835927050092337 1103 36-37 0.31981222154166034 1103 38-39 0.24782992633572576 1103 40-41 0.29964350559205144 1103 42-43 0.3232134898390058 1103 44-45 0.379356259728695 1103 46-47 0.4116133197989882 1103 48-49 0.20643147591180622 1103 50-51 0.3969086780059925 1103 52-53 0.3488614375123049 1103 54-55 0.35638963907057786 1103 56-57 0.22472733874667483 1103 58-59 0.32341449466015604 1103 60-61 0.30445423476919586 1103 62-63 0.3670457873402526 1103 64-65 0.3143909460831935 1103 66-67 0.3046115428160121 1103 68-69 0.26597514316777193 1103 70-71 0.2758188227363121 1103 72-73 0.22395486394844255 1103 74-75 0.26651892808500577 1103 76-77 0.21021430587089895 1103 78-79 0.2883123483847001 1103 80-81 0.22497316731608663 1103 82-83 0.2345121546064206 1103 84-85 0.21341496909700908 1103 86-87 0.37762850423747807 1103 88-89 0.3493227931446583 1103 90-91 0.2514764681148094 1103 92-93 0.13601488842166276 1103 94-95 0.2556530314547132 1103 96-97 0.1531077320035905 1103 98-99 0.11071838218372676 1103 100-101 -0.05408468085725815 1104 1 0.0129278519139433 1104 2 0.044588901295789185 1104 3 0.11086113687098731 1104 4 0.0628414058162079 1104 5 -0.04047166605875674 1104 6 -0.0021688528795920092 1104 7 -0.009808678514708902 1104 8 0.10205078614467311 1104 9 0.14017409106403989 1104 10-11 0.2115241956528564 1104 12-13 0.311692032669896 1104 14-15 0.2847889048294334 1104 16-17 0.32806124233408696 1104 18-19 0.4046488520252254 1104 20-21 0.42180682131821357 1104 22-23 0.37126978864571925 1104 24-25 0.34993740263518447 1104 26-27 0.3397290271199722 1104 28-29 0.41297650172006684 1104 30-31 0.33447096398862897 1104 32-33 0.41265628780872987 1104 34-35 0.3561859328449799 1104 36-37 0.4030593141774972 1104 38-39 0.3441805831363851 1104 40-41 0.3451198767998278 1104 42-43 0.3288472382479455 1104 44-45 0.3477106558796308 1104 46-47 0.29708103796477303 1104 48-49 0.24107416129068326 1104 50-51 0.22231520680343664 1104 52-53 0.2056099410961636 1104 54-55 0.3041082684132803 1104 56-57 0.27001384521616956 1104 58-59 0.31436800966874046 1104 60-61 0.3268764298219011 1104 62-63 0.3880755765961865 1104 64-65 0.37480888028530757 1104 66-67 0.2491818555423535 1104 68-69 0.31775278316645483 1104 70-71 0.2362707510319524 1104 72-73 0.22543920831633102 1104 74-75 0.38671199908437615 1104 76-77 0.2832123724568305 1104 78-79 0.29376511687526374 1104 80-81 0.2471798089121009 1104 82-83 0.14589727871383218 1104 84-85 0.24806460781318407 1104 86-87 0.1231027508144038 1104 88-89 0.26246073769951295 1104 90-91 0.15302976641104493 1104 92-93 0.15222794640283865 1104 94-95 0.1398712735351566 1104 96-97 0.05208868179083481 1104 98-99 0.2897762822372272 1104 100-101 0.20713964732481216 1105 1 0.26880574492643916 1105 2 0.20004693163068055 1105 3 0.2978693754769566 1105 4 0.3089263154793187 1105 5 0.25599602702089186 1105 6 0.18436639455529757 1105 7 0.2059037044827008 1105 8 0.20387375757704262 1105 9 0.3596630244726384 1105 10-11 0.28276839855942626 1105 12-13 0.3452092865170968 1105 14-15 0.3540787908614291 1105 16-17 0.35308882118746965 1105 18-19 0.3740623355941253 1105 20-21 0.39829266663312524 1105 22-23 0.457159746253609 1105 24-25 0.39545394810247814 1105 26-27 0.2845266776599331 1105 28-29 0.32539491812200794 1105 30-31 0.3893942043839971 1105 32-33 0.3332605506324313 1105 34-35 0.3970103837583068 1105 36-37 0.4820182170741063 1105 38-39 0.5015250439375194 1105 40-41 0.4235017452256358 1105 42-43 0.42278081287319225 1105 44-45 0.5128985794779126 1105 46-47 0.574371189639244 1105 48-49 0.5290123330063992 1105 50-51 0.5545457549492951 1105 52-53 0.4569818737558009 1105 54-55 0.38504768348332163 1105 56-57 0.40197284772871456 1105 58-59 0.5848198970215748 1105 60-61 0.5861766832890893 1105 62-63 0.495197008409086 1105 64-65 0.4732312591873864 1105 66-67 0.5314469003141795 1105 68-69 0.4678278640582718 1105 70-71 0.5540376202510018 1105 72-73 0.5400408737470244 1105 74-75 0.5590873961645215 1105 76-77 0.45950193750417867 1105 78-79 0.4542284785068986 1105 80-81 0.4973663120610752 1105 82-83 0.3708542040498486 1105 84-85 0.38704044541709237 1105 86-87 0.3899649598528967 1105 88-89 0.45880132823748454 1105 90-91 0.4103317982069754 1105 92-93 0.4782098089254845 1105 94-95 0.5257335600526929 1105 96-97 0.47765769164543315 1105 98-99 0.431918614947044 1105 100-101 0.46375174660130725 1106 1 0.2315799023208882 1106 2 0.2934428355262426 1106 3 0.23467721947113773 1106 4 0.2318281280989325 1106 5 0.1373009862607404 1106 6 0.21605780866644864 1106 7 0.24193158985380592 1106 8 0.21002883999607747 1106 9 0.2781092397524958 1106 10-11 0.22021910045170046 1106 12-13 0.22876477706630993 1106 14-15 0.30395878367157536 1106 16-17 0.31550066612619787 1106 18-19 0.2927802852157768 1106 20-21 0.37663110453073045 1106 22-23 0.2947373279941914 1106 24-25 0.32301242737700164 1106 26-27 0.3534569748054892 1106 28-29 0.29927564401572226 1106 30-31 0.3755383962827139 1106 32-33 0.26415332658729085 1106 34-35 0.20642874538702216 1106 36-37 0.29318822549752355 1106 38-39 0.3149538661048368 1106 40-41 0.37893484606340166 1106 42-43 0.28943167829948635 1106 44-45 0.35939651944259055 1106 46-47 0.34093870205408194 1106 48-49 0.4599715446596093 1106 50-51 0.32131838978556004 1106 52-53 0.31985894480499866 1106 54-55 0.42637456474140123 1106 56-57 0.3736384629373788 1106 58-59 0.49584839916972356 1106 60-61 0.49289884814125884 1106 62-63 0.5354570416093125 1106 64-65 0.5028224593650208 1106 66-67 0.4714725582390642 1106 68-69 0.44910075089202195 1106 70-71 0.4999549086876627 1106 72-73 0.48298423951695213 1106 74-75 0.41975252852487444 1106 76-77 0.4341168042979646 1106 78-79 0.45509919146591216 1106 80-81 0.4340941512620162 1106 82-83 0.3154529993961859 1106 84-85 0.46130410174188086 1106 86-87 0.3323516402883726 1106 88-89 0.33470032482934897 1106 90-91 0.34806656998184593 1106 92-93 0.20585921875797197 1106 94-95 0.2524361290171768 1106 96-97 0.27887062775085525 1106 98-99 0.3545916913956759 1106 100-101 0.324301277322089 1107 1 0.2974790106399112 1107 2 0.24744872668004092 1107 3 0.21341854923827697 1107 4 0.1823909508344599 1107 5 0.1762534713159738 1107 6 0.22036039295305443 1107 7 0.21112048181239373 1107 8 0.26085639094975477 1107 9 0.3298790549966242 1107 10-11 0.21715540685575974 1107 12-13 0.19345021961688502 1107 14-15 0.28033444282282005 1107 16-17 0.18854206436098764 1107 18-19 0.22254088869201638 1107 20-21 0.2969168468441339 1107 22-23 0.24729512176109836 1107 24-25 0.2190065028569066 1107 26-27 0.15035047224762366 1107 28-29 0.2844142943355692 1107 30-31 0.27617472497546913 1107 32-33 0.29487134265382764 1107 34-35 0.3743438121396494 1107 36-37 0.4033904386174143 1107 38-39 0.3398130936883561 1107 40-41 0.30101169131984307 1107 42-43 0.4522073339806312 1107 44-45 0.38007545022620803 1107 46-47 0.4326953576297896 1107 48-49 0.3703656807327107 1107 50-51 0.37026024433785665 1107 52-53 0.3254361217224542 1107 54-55 0.32905524658840335 1107 56-57 0.33867291697796986 1107 58-59 0.3211502974452145 1107 60-61 0.379650959227007 1107 62-63 0.33007011933010233 1107 64-65 0.28245974099927196 1107 66-67 0.36434504959385094 1107 68-69 0.4120703315043315 1107 70-71 0.4658693988706304 1107 72-73 0.3474087909737946 1107 74-75 0.2971999465739046 1107 76-77 0.4128669761344028 1107 78-79 0.2741627596045717 1107 80-81 0.35343512681252065 1107 82-83 0.39166496348336466 1107 84-85 0.42111956608732015 1107 86-87 0.4842023354418572 1107 88-89 0.5902194443861362 1107 90-91 0.4886778272373462 1107 92-93 0.5086163427965218 1107 94-95 0.4818044115469817 1107 96-97 0.3310573549039546 1107 98-99 0.37800365574978656 1107 100-101 0.4087420381270981 1108 1 0.1971984314821782 1108 2 0.08971118289468905 1108 3 0.11478129926695146 1108 4 0.07801859229311958 1108 5 0.15869080422579174 1108 6 0.0928181957888441 1108 7 0.14422705777611355 1108 8 0.16787704616385213 1108 9 0.26859280249303197 1108 10-11 0.16499238860046717 1108 12-13 0.22256913047565519 1108 14-15 0.195545274237098 1108 16-17 0.08425683535369188 1108 18-19 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48-49 -0.6297194169767764 2316 50-51 -0.6650990004836714 2316 52-53 -0.6303235154657543 2316 54-55 -0.6306597779982113 2316 56-57 -0.6150479421522022 2316 58-59 -0.7348349505865741 2316 60-61 -0.7336120538433946 2316 62-63 -0.700052941130032 2316 64-65 -0.9434463967367179 2316 66-67 -0.6489406392135066 2316 68-69 -0.5132203765391807 2316 70-71 -0.876292057472206 2316 72-73 -0.6084251156225591 2316 74-75 -0.4262224336141287 2316 76-77 -0.48429944848788153 2316 78-79 -0.4760150793212645 2316 80-81 -0.32045108684896917 2316 82-83 -0.44211424108235065 2316 84-85 -0.3343071963779707 2316 86-87 -0.3658070915499181 2316 88-89 -0.3368502898746115 2316 90-91 -0.19786170243219914 2316 92-93 -0.18631754086930385 2316 94-95 -0.223587572962181 2316 96-97 -0.1856397583983025 2316 98-99 -0.14883358851121287 2316 100-101 -0.12509169012075816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 64.0 7 1161.0 8 2267.0 9 1739.0 10 1827.0 11 2246.0 12 2590.0 13 2743.0 14 2712.0 15 2852.0 16 3014.0 17 3628.0 18 4582.0 19 5769.0 20 7190.0 21 8925.0 22 9886.0 23 9981.0 24 9454.0 25 9182.0 26 9737.0 27 10680.0 28 12385.0 29 14827.0 30 18212.0 31 22536.0 32 28674.0 33 38870.0 34 57390.0 35 93433.0 36 179086.0 37 396504.0 38 605821.0 39 209513.0 40 223.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.59069800966976 19.617947782397415 13.570687426908263 24.220666781024562 2 14.984106301436606 21.230002966972734 40.729048339305464 23.0568423922852 3 17.457924583017405 26.58625481434629 31.129075606399496 24.826744996236805 4 12.253932635750179 18.020867149465584 39.630095049290304 30.09510516549394 5 12.927284582972707 38.185833068391794 35.86438643730273 13.022495911332774 6 29.351797477011548 38.54393717840334 19.040868792196246 13.063396552388859 7 25.849261022638952 33.28624917095183 23.83244594214794 17.032043864261276 8 24.696723422824903 35.852596771643114 21.650352041651605 17.800327763880375 9 26.240610872306746 15.909678868504997 20.9086647337575 36.94104552543075 10-11 23.711112961200826 26.433547912698362 30.205067544726695 19.650271581374117 12-13 24.750894422147137 24.402177344509116 29.427396612734068 21.41953162060968 14-15 22.170829461648108 25.809757261400357 26.974922654764505 25.044490622187034 16-17 21.811915161342412 28.598013189897987 27.575161912339645 22.014909736419956 18-19 21.733522265984913 28.03297530372358 28.98190370134039 21.251598728951116 20-21 22.742432682964715 27.40831858693873 28.628688670690057 21.220560059406505 22-23 22.54376843532139 27.219236934843376 28.248430046773127 21.988564583062107 24-25 22.191754721314094 27.309503308649536 28.452905314457205 22.045836655579166 26-27 22.19513517047242 27.5616680533027 28.310423573073294 21.932773203151584 28-29 22.184211570299652 27.654448810780334 28.326459753378074 21.834879865541936 30-31 22.46157602686032 27.47578788212346 28.257593578375857 21.805042512640366 32-33 22.21687062043255 27.550241576395635 28.232840868009944 22.00004693516187 34-35 22.461352526089524 27.569267079509842 28.16467313291647 21.804707261484168 36-37 22.49468207853482 27.433238922882737 28.14369199805778 21.92838700052467 38-39 22.230001290716952 27.568428951619346 28.1862968324912 22.0152729251725 40-41 22.322195358671244 27.457963695652293 28.238037260931005 21.981803684745458 42-43 22.273751566600716 27.368814825700127 28.100640162082758 22.256793445616395 44-45 22.326385998123712 27.50179778432511 27.98425213568955 22.187564081861627 46-47 22.294003299992568 27.42871574637328 27.883260946940297 22.394020006693854 48-49 22.236209684538956 27.503159750974742 27.9302988548906 22.330331709595697 50-51 22.13722053323931 27.635954121996782 27.84297171094869 22.38385363381522 52-53 22.19991249944823 27.59645036075818 27.80243425864515 22.401202881148436 54-55 22.13367245850289 27.476402509243155 27.849313545320086 22.540611486933866 56-57 22.222178763739013 27.485174914496984 27.704680609017252 22.58796571274675 58-59 22.082434906797385 27.389097520650076 27.86166196290669 22.666805609645845 60-61 22.162308494761422 27.396109857333872 27.83987063775386 22.601711010150847 62-63 22.077322326665374 27.331881323325714 27.828108909690602 22.76268744031831 64-65 22.158201668098002 27.194707725248268 27.780922309455814 22.866168297197913 66-67 22.185161448575546 27.299836900312506 27.576586729753483 22.93841492135846 68-69 22.122944421504574 27.288550111387195 27.635143931702633 22.953361535405595 70-71 22.271432746103685 26.894518252469823 27.677860516521456 23.156188484905037 72-73 22.306326803944565 26.974643278801008 27.55264420968172 23.166385707572708 74-75 22.197845117318348 27.076419942303275 27.572451965493716 23.15328297488466 76-77 22.161135115714732 26.998474048487374 27.625086397016712 23.215304438781185 78-79 22.277690767686035 27.059741197282456 27.45570075034796 23.20686728468355 80-81 22.25866526457183 27.04602383747471 27.393679286451437 23.301631611502017 82-83 22.246568285352375 26.954444396640113 27.489589054720255 23.30939826328726 84-85 22.387988398074988 26.735329828468746 27.44301708160516 23.433664691851106 86-87 22.19996837464093 26.824059634475667 27.524203736597634 23.451768254285767 88-89 22.432716489830995 26.556193960673923 27.474977691829316 23.53611185766577 90-91 22.611433293680573 26.52937386817813 27.48254878044011 23.376644057701192 92-93 22.646103850750656 26.48489721478927 27.497467456890888 23.37153147756918 94-95 22.53507984285661 26.299391575026693 27.550157763606585 23.615370818510108 96-97 22.508371500746215 26.165011736584226 27.65567806501973 23.670938697649834 98-99 22.625178591084666 26.11237730506123 27.552923585645217 23.709520518208887 100-101 23.004263835954905 26.07874043905609 27.380213365010842 23.536782359978165 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 101.0 1 73.5 2 75.5 3 104.5 4 87.0 5 95.0 6 141.5 7 270.0 8 509.5 9 632.0 10 587.5 11 544.0 12 589.0 13 675.5 14 743.5 15 807.0 16 905.5 17 1053.0 18 1180.0 19 1346.5 20 1553.0 21 1839.0 22 2202.0 23 2737.0 24 3591.5 25 4775.5 26 6576.0 27 9155.5 28 12505.5 29 16889.5 30 21137.5 31 25362.0 32 30692.5 33 35920.0 34 40451.5 35 43839.5 36 48623.5 37 53218.0 38 55042.0 39 57290.0 40 59854.0 41 61493.0 42 62293.0 43 64134.0 44 66870.0 45 69120.0 46 71063.0 47 72431.5 48 73181.5 49 72846.5 50 70844.0 51 67730.5 52 64540.5 53 62108.0 54 58481.5 55 52806.5 56 46470.5 57 39814.0 58 34369.0 59 29925.0 60 24603.5 61 19712.5 62 15829.0 63 12105.5 64 8858.5 65 6321.0 66 4655.5 67 3501.5 68 2546.5 69 1874.0 70 1432.5 71 1058.0 72 785.0 73 527.0 74 376.0 75 282.5 76 208.0 77 148.5 78 109.5 79 84.5 80 72.0 81 68.5 82 56.0 83 49.0 84 44.5 85 31.5 86 22.0 87 21.0 88 21.0 89 15.0 90 7.5 91 6.5 92 5.0 93 2.5 94 4.0 95 4.5 96 3.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.1175038539914164E-4 48-49 2.7937596349785415E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1789703.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.26400149343243 #Duplication Level Percentage of deduplicated Percentage of total 1 66.84348616603295 34.935080608077755 2 16.061697787713168 16.788971943282025 3 6.954763888681067 10.904513707934917 4 3.5523992804269424 7.42650405190008 5 2.041657949606288 5.3352607063650614 6 1.247224865584676 3.911097734253814 7 0.8412916949424951 3.0778489280620804 8 0.5733939018000839 2.39742877920037 9 0.3955363088883361 1.8605079214602072 >10 1.4552574053953153 11.50624332908987 >50 0.024376380289886354 0.8503943022416572 >100 0.008592295494284854 0.6390777674291203 >500 0.0 0.0 >1k 3.220751444721589E-4 0.36707022070306156 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2920 0.1631555626827468 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2293 0.1281218168601159 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2350077079828329E-4 2 0.0 0.0 0.0 0.0 2.2350077079828329E-4 3 0.0 0.0 0.0 0.0 2.2350077079828329E-4 4 0.0 0.0 0.0 0.0 2.2350077079828329E-4 5 0.0 0.0 0.0 0.0 2.793759634978541E-4 6 0.0 0.0 0.0 0.0 3.3525115619742495E-4 7 0.0 0.0 0.0 0.0 3.3525115619742495E-4 8 0.0 0.0 0.0 5.587519269957082E-5 3.3525115619742495E-4 9 0.0 0.0 0.0 5.587519269957082E-5 3.3525115619742495E-4 10-11 0.0 0.0 0.0 5.587519269957082E-5 3.9112634889699576E-4 12-13 0.0 0.0 0.0 5.587519269957082E-5 7.543151014442061E-4 14-15 0.0 0.0 0.0 1.6762557809871248E-4 8.381278904935624E-4 16-17 0.0 0.0 0.0 1.6762557809871248E-4 8.940030831931331E-4 18-19 0.0 0.0 0.0 3.073135598476395E-4 0.0010057534685922749 20-21 0.0 0.0 0.0 4.74939137946352E-4 0.0010616286612918457 22-23 0.0 0.0 0.0 7.263775050944208E-4 0.0010616286612918457 24-25 0.0 0.0 0.0 0.001564505395587983 0.0010616286612918457 26-27 0.0 0.0 0.0 0.0029613852130772537 0.0012013166430407726 28-29 0.0 0.0 0.0 0.009079718813680259 0.001285129432090129 30-31 0.0 0.0 0.0 0.03237967416940129 0.0013130670284399143 32-33 0.0 0.0 0.0 0.08764023974927684 0.0013410046247896998 34-35 0.0 0.0 0.0 0.17544810507665237 0.0013968798174892705 36-37 0.0 0.0 0.0 0.3175666577080108 0.0014527550101888413 38-39 0.0 0.0 0.0 0.5201421688403047 0.0015086302028884122 40-41 0.0 0.0 0.0 0.7252041260477298 0.0015086302028884122 42-43 0.0 0.0 5.587519269957082E-5 0.9193704206787383 0.0015086302028884122 44-45 0.0 0.0 5.587519269957082E-5 1.1505819680695624 0.0015365677992381977 46-47 0.0 0.0 5.587519269957082E-5 1.4448486704218522 0.001564505395587983 48-49 0.0 0.0 5.587519269957082E-5 1.750932976030101 0.001564505395587983 50-51 0.0 0.0 5.587519269957082E-5 2.0486360027334145 0.001564505395587983 52-53 0.0 0.0 5.587519269957082E-5 2.322005383016065 0.0017880061663862663 54-55 0.0 0.0 5.587519269957082E-5 2.6196804721230285 0.0018718189554356225 56-57 0.0 0.0 5.587519269957082E-5 2.9730910659478136 0.001899756551785408 58-59 0.0 0.0 5.587519269957082E-5 3.369609370940318 0.001899756551785408 60-61 0.0 0.0 5.587519269957082E-5 3.751236937078387 0.001983569340834764 62-63 0.0 0.0 5.587519269957082E-5 4.119957333702855 0.0020673821298841204 64-65 0.0 0.0 5.587519269957082E-5 4.495550379029369 0.0020673821298841204 66-67 0.0 0.0 5.587519269957082E-5 4.930399066213779 0.002095319726233906 68-69 0.0 0.0 5.587519269957082E-5 5.426179651037072 0.0021232573225836915 70-71 0.0 0.0 5.587519269957082E-5 5.916456529379456 0.0021511949189334766 72-73 0.0 0.0 5.587519269957082E-5 6.404526337610207 0.0022070701116330476 74-75 0.0 0.0 5.587519269957082E-5 6.886952751378301 0.0022350077079828327 76-77 0.0 0.0 5.587519269957082E-5 7.400389897094658 0.002290882900682404 78-79 0.0 5.587519269957082E-5 5.587519269957082E-5 7.957772881869227 0.002290882900682404 80-81 0.0 5.587519269957082E-5 5.587519269957082E-5 8.538372009210466 0.002290882900682404 82-83 0.0 5.587519269957082E-5 5.587519269957082E-5 9.138555391592906 0.0023467580933819745 84-85 0.0 5.587519269957082E-5 5.587519269957082E-5 9.742007472748272 0.0024026332860815455 86-87 0.0 5.587519269957082E-5 5.587519269957082E-5 10.393344594047168 0.0025143836714806873 88-89 0.0 5.587519269957082E-5 5.587519269957082E-5 11.119163347214595 0.0026261340568798286 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1980 0.0 49.419094 1 GTATCAA 3480 0.0 43.405083 1 CAACGCA 4470 0.0 33.473087 5 TCAACGC 4445 0.0 33.447624 4 ATCAACG 4525 0.0 32.85629 3 AACGCAG 4550 0.0 32.57136 6 TATCAAC 4750 0.0 31.299934 2 ACGCAGA 5135 0.0 28.953201 7 CGCAGAG 5165 0.0 28.32521 8 TAGGACG 90 8.949613E-4 26.388834 4 GTACATG 4680 0.0 26.28734 1 TACATGG 4580 0.0 25.72047 2 ACATGGG 4840 0.0 24.142511 3 GCAGAGT 6120 0.0 23.439493 9 AGAGTAC 5345 0.0 22.305847 10-11 TCGCCGG 225 0.0 20.055513 94-95 GAGTACT 3345 0.0 19.667374 12-13 TATACCG 145 6.147959E-4 19.655132 5 CATGGGG 3100 0.0 19.612862 4 CAGAGTA 5835 0.0 19.333721 10-11 >>END_MODULE