FastQCFastQC Report
Fri 10 Feb 2017
SRR2031539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1789703
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT82340.46007633668826614No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34110.19059028229823607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27240.15220402491363091No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT26660.14896326373705582RNA PCR Primer, Index 31 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT17990.1005194716665279RNA PCR Primer, Index 31 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.055.805371
GTATCAA36550.040.9942931
TCAACGC45000.032.9323734
CAACGCA45350.032.7829485
ATCAACG45900.032.3901253
AACGCAG46900.031.800786
TATCAAC47850.031.3679492
ACGCAGA52850.028.1306847
GTACATG46450.027.5465221
CGCAGAG53900.027.230198
CTATACG1056.909319E-527.1420674
TACATGG45450.026.9629122
ACATGGG46450.025.8711533
GCAGAGT62250.023.2724139
CATGGGG28700.022.1770554
GTATAGG3950.021.6758231
GAGTACT37550.021.50403412-13
CAGAGTA60950.021.11915410-11
AGAGTAC55700.019.99717310-11
GGACCGT1456.14908E-419.6545986
Produced by FastQC (version 0.11.5)