FastQCFastQC Report
Fri 10 Feb 2017
SRR2031533_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031533_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678932
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35000.5155155450030342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28150.41462178833815466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.21519091749983799No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16050.061.853581
ACCGCTA405.3866126E-447.5000045
GTATCAA33100.042.9078561
TCAACGC39250.036.0636944
CAACGCA39650.035.6998755
AACGCAG40500.035.4197546
ATCAACG40050.035.224723
TATCAAC41350.034.117292
ACGCAGA44950.031.8075667
GTAATAC1201.504286E-731.6666683
CCGCGAA300.00395120331.66666886-87
CGCAGAG45200.031.631648
GCAGAGT48850.028.4902769
GTATAAA2800.027.1428591
GTACATG34100.026.8841651
GAGTACT29300.026.01962712-13
TACATGG35550.025.5203932
ACATGGG34550.025.2966733
GTACTTT31050.024.32367314-15
CAGAGTA48600.023.70113210-11
Produced by FastQC (version 0.11.5)