FastQCFastQC Report
Fri 10 Feb 2017
SRR2031533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678932
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT87431.2877578314175793No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40030.5896024933277559No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33140.4881195760400158No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT23810.3506978607577784RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18270.2690991144915838No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT16660.24538539942144427RNA PCR Primer, Index 31 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18400.061.25711
CTAACGC250.005020932356.9984553
GTATCAA35700.041.297091
TCAACGC44650.032.871454
ATCAACG44700.032.834683
AACGCAG45150.032.7178276
CAACGCA45150.032.6126255
TATCAAC46200.031.768622
ACGCAGA50150.029.455837
CGCAGAG50300.029.273568
GCAGAGT53900.026.7896259
GTACATG38500.024.458531
ATAGCCC3750.024.0660133
GAGTACT31800.023.8240412-13
CAGAGTA53550.023.59413110-11
TACATGG40500.023.3388732
ACATGGG38900.023.321993
GTACTTT32950.022.77631814-15
CTGTGCG1050.00219564922.6184339
AGAGTAC52400.022.20836610-11
Produced by FastQC (version 0.11.5)