##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031533_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	678932
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77634284434966	34.0	31.0	34.0	31.0	34.0
2	32.92584087949898	34.0	33.0	34.0	31.0	34.0
3	33.022382212062475	34.0	33.0	34.0	31.0	34.0
4	36.39133226891647	37.0	37.0	37.0	35.0	37.0
5	36.32788114273595	37.0	37.0	37.0	35.0	37.0
6	36.3495593078541	37.0	37.0	37.0	35.0	37.0
7	36.37385776484243	37.0	37.0	37.0	35.0	37.0
8	36.403583864068864	37.0	37.0	37.0	35.0	37.0
9	38.26804598987822	39.0	39.0	39.0	37.0	39.0
10-11	38.234234798183024	39.0	39.0	39.0	37.0	39.0
12-13	38.244493557528585	39.0	39.0	39.0	37.0	39.0
14-15	39.78151199236448	41.0	40.0	41.0	37.5	41.0
16-17	39.753674889385096	41.0	40.0	41.0	37.5	41.0
18-19	39.80367768789805	41.0	40.0	41.0	38.0	41.0
20-21	39.8110664985595	41.0	40.0	41.0	38.0	41.0
22-23	39.784063794312246	41.0	40.0	41.0	37.0	41.0
24-25	39.734757678235816	41.0	40.0	41.0	37.0	41.0
26-27	39.63018387703039	41.0	40.0	41.0	37.0	41.0
28-29	39.54538451568051	41.0	40.0	41.0	37.0	41.0
30-31	39.39143316267314	41.0	39.0	41.0	36.0	41.0
32-33	38.497538781498	40.0	38.0	41.0	35.0	41.0
34-35	38.66302369014865	40.0	38.0	41.0	35.0	41.0
36-37	38.829679555537226	40.0	38.0	41.0	35.0	41.0
38-39	38.74552679797093	40.0	38.0	41.0	35.0	41.0
40-41	38.671117284205195	40.0	38.0	41.0	35.0	41.0
42-43	38.467762603618624	40.0	38.0	41.0	35.0	41.0
44-45	38.28013482940854	40.0	38.0	41.0	34.5	41.0
46-47	37.985119275568096	40.0	38.0	41.0	33.5	41.0
48-49	37.773062692581874	40.0	38.0	41.0	33.0	41.0
50-51	37.71358913705643	40.0	38.0	41.0	33.0	41.0
52-53	37.84510525354528	41.0	38.0	41.0	33.0	41.0
54-55	37.77589066357161	41.0	38.0	41.0	33.0	41.0
56-57	37.61428243181939	41.0	38.0	41.0	33.0	41.0
58-59	37.491018982755264	40.0	38.0	41.0	32.0	41.0
60-61	37.27640544266583	40.0	37.0	41.0	31.5	41.0
62-63	36.98232591776496	40.0	37.0	41.0	31.0	41.0
64-65	36.729627562112256	39.5	36.0	41.0	31.0	41.0
66-67	36.37768289018635	39.0	36.0	41.0	29.5	41.0
68-69	35.905902947570596	39.0	35.0	41.0	28.5	41.0
70-71	35.42868799820895	38.5	35.0	40.5	26.5	41.0
72-73	34.981362640146585	37.0	35.0	39.5	26.0	41.0
74-75	34.51239814885732	37.0	35.0	39.0	26.0	41.0
76-77	33.05127906771223	35.5	33.0	38.0	23.0	39.0
78-79	33.66894549085917	36.0	34.5	37.5	25.0	39.0
80-81	33.45672541580011	35.0	35.0	37.0	25.0	39.0
82-83	33.155455921948004	35.0	34.5	37.0	25.0	39.0
84-85	32.83269237567238	35.0	34.0	36.0	24.0	37.5
86-87	32.57628672680033	35.0	34.0	36.0	24.0	37.0
88-89	32.35139380674353	35.0	34.0	36.0	23.5	37.0
90-91	32.172951046643846	35.0	34.0	35.0	23.0	36.0
92-93	32.032710934232	35.0	34.0	35.0	22.0	36.0
94-95	31.92438123405584	35.0	34.0	35.0	20.0	36.0
96-97	31.82131126534027	35.0	34.0	35.0	20.0	36.0
98-99	31.76430776572617	35.0	34.0	35.0	20.0	35.5
100-101	30.63522340970819	34.5	31.5	35.0	17.5	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.23998625030196763
1101	2	0.18384933485207
1101	3	0.18557667226507846
1101	4	0.022981156774356748
1101	5	0.14656070611651728
1101	6	0.22877656921453138
1101	7	0.08478665783867712
1101	8	0.1463901498074165
1101	9	0.23483645824729393
1101	10-11	0.32008905448875424
1101	12-13	0.18863312096105034
1101	14-15	0.25156811537586066
1101	16-17	-0.01531335124964528
1101	18-19	0.16555022528662278
1101	20-21	0.40157727721850023
1101	22-23	0.35879220080830265
1101	24-25	0.3139030011461088
1101	26-27	0.3520623215045475
1101	28-29	0.3207702816094766
1101	30-31	0.28698454545799734
1101	32-33	0.42510652802850757
1101	34-35	-0.11490820224236131
1101	36-37	0.10837547451307472
1101	38-39	0.16689579967793833
1101	40-41	0.21293080049645852
1101	42-43	0.2784864095938886
1101	44-45	0.30962465210172496
1101	46-47	0.4435891478937535
1101	48-49	0.4041186372546264
1101	50-51	0.4223335506830921
1101	52-53	0.3549658563692901
1101	54-55	0.4280720956473374
1101	56-57	0.4177325092157034
1101	58-59	0.1342292507714049
1101	60-61	0.32956719232690546
1101	62-63	0.2538307705668359
1101	64-65	0.24489171010073818
1101	66-67	0.20021051024199465
1101	68-69	0.19600634506229397
1101	70-71	0.2320611042197953
1101	72-73	0.3481693505155974
1101	74-75	0.43091038202149434
1101	76-77	0.4978031532058935
1101	78-79	0.3721509861912651
1101	80-81	0.4514467646184315
1101	82-83	0.5212212441055399
1101	84-85	0.5369627757265434
1101	86-87	0.42122451598276456
1101	88-89	0.5528695792522385
1101	90-91	0.4873533986046823
1101	92-93	0.44929256040200727
1101	94-95	0.30396393240812003
1101	96-97	0.34264913671171016
1101	98-99	0.18265526813077315
1101	100-101	-0.2141165404021912
1102	1	-0.16942011314026928
1102	2	0.04060960248677503
1102	3	0.009158421855794074
1102	4	-0.033980192787886665
1102	5	-0.20058143923191096
1102	6	-0.06140422657163924
1102	7	-0.12959692818168378
1102	8	-0.07934655743153485
1102	9	-0.22960940978602196
1102	10-11	-0.043019529752115204
1102	12-13	0.12866443991612897
1102	14-15	0.13578140457794063
1102	16-17	0.2182175050390569
1102	18-19	0.2818396409033923
1102	20-21	0.24431519450799044
1102	22-23	0.2525746764293402
1102	24-25	0.2001093147627344
1102	26-27	0.034997939698008906
1102	28-29	-0.025793530761511363
1102	30-31	0.02722406428495816
1102	32-33	-0.028600141485341624
1102	34-35	-0.16113035328514513
1102	36-37	-0.09604743814974626
1102	38-39	0.01884255745429897
1102	40-41	-0.11525092062248632
1102	42-43	0.013405265028438862
1102	44-45	0.017655472800704786
1102	46-47	-0.07423685395691138
1102	48-49	-0.040721742843601305
1102	50-51	0.28907228653618233
1102	52-53	0.3261070407952218
1102	54-55	0.25804220028111047
1102	56-57	0.07907851248996423
1102	58-59	0.1819995547076374
1102	60-61	0.2728638682469011
1102	62-63	0.19207370600707918
1102	64-65	0.3274999028546688
1102	66-67	0.1894312732856278
1102	68-69	0.07817322526729242
1102	70-71	-0.0665188658848308
1102	72-73	-0.2258038860136864
1102	74-75	-0.06710549099437912
1102	76-77	-0.17594141936154983
1102	78-79	-0.0709677411202847
1102	80-81	-0.15687500205535798
1102	82-83	-0.2270615518229988
1102	84-85	-0.12447220185193686
1102	86-87	-0.1042043401635766
1102	88-89	-0.11760518763507832
1102	90-91	-0.08033380333139917
1102	92-93	-0.13532200291210827
1102	94-95	-0.001146751626325937
1102	96-97	-0.16003788727575952
1102	98-99	-0.1946269227724997
1102	100-101	-0.368549240238476
1103	1	0.4482761545559981
1103	2	0.4155667024438756
1103	3	0.3600807727781472
1103	4	0.2294046205969238
1103	5	0.20503846638799672
1103	6	0.3700200548526382
1103	7	0.26166218807743036
1103	8	0.24983619675121815
1103	9	0.1584214782448683
1103	10-11	0.3157481665155828
1103	12-13	0.2981410593927549
1103	14-15	0.3683478996444336
1103	16-17	0.26181467363623057
1103	18-19	0.3516165606803128
1103	20-21	0.2984443236371206
1103	22-23	0.1618625357171979
1103	24-25	0.2337268608802745
1103	26-27	0.28910025630032976
1103	28-29	0.3226084426404654
1103	30-31	0.3854715975324865
1103	32-33	0.503521265636067
1103	34-35	0.2936422764874891
1103	36-37	0.41674213713982766
1103	38-39	0.5017683403800746
1103	40-41	0.5150901347185695
1103	42-43	0.6530127296359041
1103	44-45	0.484504464649703
1103	46-47	0.5603244931044387
1103	48-49	0.5522158375939838
1103	50-51	0.3664049763688695
1103	52-53	0.6416422188304054
1103	54-55	0.6783124705513828
1103	56-57	0.4712492546607052
1103	58-59	0.6738163715244525
1103	60-61	0.5774219978050326
1103	62-63	0.6406687296021047
1103	64-65	0.7078355956903621
1103	66-67	0.5292049755593311
1103	68-69	0.41895507854914626
1103	70-71	0.38258023321426293
1103	72-73	0.4365727763629721
1103	74-75	0.4597392108503229
1103	76-77	0.6025924716723381
1103	78-79	0.3450875999350558
1103	80-81	0.351485143804787
1103	82-83	0.3947638450023945
1103	84-85	0.30120347382374035
1103	86-87	0.4712568352976021
1103	88-89	0.3698250997952073
1103	90-91	0.26896370846611717
1103	92-93	0.22671145912135415
1103	94-95	0.19941094893136935
1103	96-97	0.271086093848222
1103	98-99	0.1831562175106427
1103	100-101	0.294910648221407
1104	1	0.35214050417425113
1104	2	0.3372211124864961
1104	3	0.34601297881433624
1104	4	0.16369662490625814
1104	5	0.2936140436984118
1104	6	0.2619775235865376
1104	7	0.17127856355169513
1104	8	0.13942665375935093
1104	9	0.18596375188766245
1104	10-11	0.301357172401886
1104	12-13	0.2226714823633884
1104	14-15	0.30123936997090794
1104	16-17	0.2850554187140588
1104	18-19	0.16228997239358733
1104	20-21	0.1826215684729462
1104	22-23	0.2785643328349394
1104	24-25	0.21647234466562537
1104	26-27	0.23865292855663967
1104	28-29	0.21772452926435193
1104	30-31	0.1751120178488179
1104	32-33	0.46244205817904316
1104	34-35	0.3949444452073294
1104	36-37	0.15097637137406394
1104	38-39	0.06487980539795757
1104	40-41	0.286158256566587
1104	42-43	0.37532446945197506
1104	44-45	0.5181953479073087
1104	46-47	0.3535591489040897
1104	48-49	0.20773600247289892
1104	50-51	0.24627600857958498
1104	52-53	0.2393298544729845
1104	54-55	0.26483018410549164
1104	56-57	0.3006198731127796
1104	58-59	0.34016920616151225
1104	60-61	0.371571606686004
1104	62-63	0.2643653898672156
1104	64-65	0.29413940383125947
1104	66-67	0.2627459688923537
1104	68-69	0.1319767657476092
1104	70-71	0.25424947611401194
1104	72-73	0.3746885351026492
1104	74-75	0.35378733176152366
1104	76-77	0.3386472439489552
1104	78-79	0.5011932723318822
1104	80-81	0.4573979890590536
1104	82-83	0.22543975488108714
1104	84-85	0.17838741686556858
1104	86-87	0.21586938545608092
1104	88-89	0.3582414190607466
1104	90-91	0.19620776361311698
1104	92-93	0.4209625559721033
1104	94-95	0.37434498175101893
1104	96-97	0.4234054842958592
1104	98-99	0.37335091827726785
1104	100-101	0.46876266314794535
1105	1	0.2884047253378341
1105	2	0.14644632578580996
1105	3	0.1315960066049371
1105	4	0.08943449972228024
1105	5	0.21223661931479398
1105	6	0.2053860741382536
1105	7	0.14605713578896484
1105	8	0.15088196613869798
1105	9	0.09929702716061684
1105	10-11	0.1776522816860293
1105	12-13	0.17594449096502984
1105	14-15	0.20660676152138535
1105	16-17	0.1678765143714358
1105	18-19	0.21326262885904157
1105	20-21	0.2140946473276486
1105	22-23	0.23245423294206802
1105	24-25	0.18871803295687783
1105	26-27	0.23165084709212636
1105	28-29	0.19326804696840583
1105	30-31	0.2437132562766564
1105	32-33	0.5086595697693497
1105	34-35	0.24057061185803263
1105	36-37	0.19955258577942203
1105	38-39	0.0375870736862467
1105	40-41	0.060778033672796994
1105	42-43	0.26923800013253185
1105	44-45	0.06067812574798381
1105	46-47	0.06795725149096654
1105	48-49	0.11623865438061642
1105	50-51	-0.13505728991751198
1105	52-53	-0.0923792398141643
1105	54-55	-0.12679867074065498
1105	56-57	-0.018931378233496332
1105	58-59	-0.08007470548220397
1105	60-61	-0.07703044458710906
1105	62-63	-0.027546219014858764
1105	64-65	-0.046657590963626205
1105	66-67	0.006487786007973284
1105	68-69	0.022911799088781493
1105	70-71	0.12234210563945425
1105	72-73	0.3193424807407226
1105	74-75	0.20812981250725215
1105	76-77	0.33599823924896555
1105	78-79	0.4360016295425524
1105	80-81	0.39828671924957604
1105	82-83	0.34334005940775825
1105	84-85	0.4002346658197595
1105	86-87	0.4444516381406416
1105	88-89	0.2386602666203217
1105	90-91	0.3511041403552895
1105	92-93	0.5245248504020807
1105	94-95	0.36899762103060496
1105	96-97	0.2602979187384342
1105	98-99	0.5793172026163518
1105	100-101	0.5914790759456174
1106	1	0.2012047929735985
1106	2	0.13092180518068375
1106	3	0.12074522271704069
1106	4	0.14429080015187168
1106	5	0.13137489745628983
1106	6	0.10174483957426617
1106	7	0.02062714672693744
1106	8	0.07663586708401482
1106	9	0.13113867493682818
1106	10-11	0.21878195740426065
1106	12-13	0.17187709827074826
1106	14-15	0.2561090045979668
1106	16-17	0.18623988749362042
1106	18-19	0.24399726946480627
1106	20-21	0.20308666313750479
1106	22-23	0.20635766175806936
1106	24-25	0.15385787517532634
1106	26-27	0.14509821009007595
1106	28-29	0.21159443178417803
1106	30-31	0.18090314895773218
1106	32-33	0.7599369873514803
1106	34-35	0.5450874124752119
1106	36-37	0.2787366237399951
1106	38-39	0.24096463446596061
1106	40-41	0.35769109530060206
1106	42-43	0.48522064733278114
1106	44-45	0.5199024809625428
1106	46-47	0.7732951925104672
1106	48-49	0.8295370073441646
1106	50-51	0.6667104869267462
1106	52-53	0.7279553458375432
1106	54-55	0.7930053969162358
1106	56-57	0.7542074494485504
1106	58-59	0.7676550051107185
1106	60-61	0.9673145717670053
1106	62-63	1.0846366723082141
1106	64-65	0.9114122006865273
1106	66-67	0.7732183896042883
1106	68-69	0.6759931892150135
1106	70-71	0.8078239311708941
1106	72-73	0.7407476136608366
1106	74-75	0.6777558078889996
1106	76-77	0.5770034757363476
1106	78-79	0.570443555259466
1106	80-81	0.5922956704197304
1106	82-83	0.6007575358541359
1106	84-85	0.6397233817442753
1106	86-87	0.5162578299831395
1106	88-89	0.602939602310343
1106	90-91	0.5403693412282138
1106	92-93	0.5487323759372202
1106	94-95	0.6022656964233413
1106	96-97	0.8362692580850073
1106	98-99	0.6091416666822127
1106	100-101	0.6189138312151279
1107	1	0.27249572296161517
1107	2	0.2441441170677976
1107	3	0.1580694591389218
1107	4	0.06673963002495498
1107	5	0.16837389483970355
1107	6	0.14559097460960402
1107	7	0.23082204560938635
1107	8	0.1751661778951501
1107	9	0.10826434436680188
1107	10-11	0.11671192096770966
1107	12-13	0.01314210695193907
1107	14-15	0.054750581395957454
1107	16-17	0.09133489152853969
1107	18-19	0.05649818649217053
1107	20-21	0.17810072422375356
1107	22-23	0.10896079676894743
1107	24-25	0.10913017116730828
1107	26-27	0.026540021647072365
1107	28-29	0.09021791257551826
1107	30-31	0.19766518647026032
1107	32-33	0.6596704380643104
1107	34-35	0.45626536469197276
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2312	18-19	-0.07730446885724263
2312	20-21	3.6054318589151535E-4
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2312	24-25	-0.7339728980648559
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2313	2	-0.8507711994390519
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2314	1	0.05260300182333566
2314	2	-0.081093551742633
2314	3	-0.09400692051607962
2314	4	0.05204803413882075
2314	5	-0.06829087228556574
2314	6	-5.561222579615333E-4
2314	7	-0.08169774128015916
2314	8	-0.6706742787184155
2314	9	-0.4550636521597511
2314	10-11	-0.6442968259681479
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2314	60-61	-0.6166617008294253
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2314	82-83	-0.8423423393616574
2314	84-85	-1.204110474897444
2314	86-87	-1.3757403369037746
2314	88-89	-1.1936363780186667
2314	90-91	-0.7878950172374175
2314	92-93	-0.6186247755964445
2314	94-95	-0.5955421138163253
2314	96-97	-0.4561196416422284
2314	98-99	-0.5345064860330417
2314	100-101	-0.3245118080738578
2315	1	-0.3381694703345488
2315	2	-0.6658295966630092
2315	3	-0.5683725185261181
2315	4	-0.3058355872134797
2315	5	-0.22103123181992856
2315	6	-0.14153210205589062
2315	7	-0.27790934890231256
2315	8	-0.2307273041146587
2315	9	-0.899310119443534
2315	10-11	-0.45791848069318064
2315	12-13	-0.33854389770257143
2315	14-15	-0.43747487777661576
2315	16-17	-0.36224891479021437
2315	18-19	-0.4124505301686341
2315	20-21	-1.0159739823578562
2315	22-23	-0.4313534664620775
2315	24-25	-0.3523286330585833
2315	26-27	-0.35286460272715914
2315	28-29	-0.8162431519346782
2315	30-31	-0.4195816198317459
2315	32-33	-0.41061100918734894
2315	34-35	-0.4293131843729441
2315	36-37	-1.196755810051421
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2315	70-71	-0.5765980595493403
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2315	82-83	-0.14152125218982547
2315	84-85	-0.17360827096640463
2315	86-87	0.023336885109834782
2315	88-89	-0.12399896184743397
2315	90-91	-0.03143128696127917
2315	92-93	0.026767887039547134
2315	94-95	-0.30654803380947016
2315	96-97	-1.1846102072044538
2315	98-99	-0.4878427512205086
2315	100-101	-0.2502258489669238
2316	1	-0.5495720671983051
2316	2	-1.099348639792705
2316	3	-0.8131383374740508
2316	4	-0.5513735962538604
2316	5	-0.39953622570003233
2316	6	-0.18746934489929856
2316	7	-0.22576461973358164
2316	8	-0.31937933681863484
2316	9	-0.35119806659346153
2316	10-11	-0.409243420589128
2316	12-13	-0.44822312763312055
2316	14-15	-0.4905664042005
2316	16-17	-0.5684179068631607
2316	18-19	-0.5060641723472727
2316	20-21	-1.0241983831037729
2316	22-23	-0.6435265246345026
2316	24-25	-0.4668231021029854
2316	26-27	-0.37260039846329107
2316	28-29	-0.3381018941637919
2316	30-31	-0.39138861379943535
2316	32-33	-0.4973729049486053
2316	34-35	-0.4896886531614797
2316	36-37	-0.5688742156056534
2316	38-39	-0.6163237111164577
2316	40-41	-0.730849080911149
2316	42-43	-0.7612872694026223
2316	44-45	-0.7229786855228824
2316	46-47	-0.8594376382905651
2316	48-49	-0.9440574901628125
2316	50-51	-1.120114200225018
2316	52-53	-0.7895345288884954
2316	54-55	-0.8407660530095313
2316	56-57	-1.0098366337891136
2316	58-59	-0.9377828477588679
2316	60-61	-0.9237086953213947
2316	62-63	-1.3539720157323458
2316	64-65	-0.7909241031760601
2316	66-67	-1.019805187218175
2316	68-69	-0.8984524036395527
2316	70-71	-0.6848931099261009
2316	72-73	-0.6362492452317312
2316	74-75	-0.6704770245953711
2316	76-77	-0.7749773072848427
2316	78-79	-0.7187100290706852
2316	80-81	-0.7301257093108617
2316	82-83	-0.7943503492345272
2316	84-85	-0.9176952541464942
2316	86-87	-0.8120442886652128
2316	88-89	-0.9411385853413705
2316	90-91	-0.8576700757984028
2316	92-93	-0.7621960182599778
2316	94-95	-0.8443986023488321
2316	96-97	-0.782842007144982
2316	98-99	-0.8221520790463188
2316	100-101	-0.7503295759942681
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	7.0
14	59.0
15	149.0
16	446.0
17	1071.0
18	1889.0
19	2904.0
20	4204.0
21	6292.0
22	8006.0
23	6994.0
24	5392.0
25	4990.0
26	5309.0
27	6236.0
28	8010.0
29	8479.0
30	6758.0
31	7430.0
32	9155.0
33	11850.0
34	16213.0
35	24615.0
36	42769.0
37	115832.0
38	273322.0
39	100484.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.50115346718666	21.99523272896766	14.832526004637469	22.67108779920821
2	14.326618866101464	22.488850135212363	42.33988087172206	20.844650126964115
3	15.774333806625702	29.863226361402912	33.87408459168223	20.48835524028916
4	10.686195377445754	20.967637406986267	41.48839648153276	26.85777073403522
5	10.72640558995599	39.644471022134766	37.42171528223739	12.207408105671849
6	24.731784626442707	41.56101052830033	20.438718457813152	13.26848638744381
7	22.483105819139475	35.48499702473886	24.970247388545538	17.061649767576135
8	23.148120872193388	35.95028662664303	23.32752028185444	17.57407221930915
9	25.52906623932883	15.631462355582002	22.26379077727961	36.57568062780956
10-11	21.309129633011846	28.088085404723888	31.86718257498542	18.735602387278842
12-13	23.380692028067614	26.553321982172	30.367842434883023	19.69814355487737
14-15	20.984944000282795	27.58575232865736	28.407263172158626	23.022040498901216
16-17	19.413431683879974	30.779371129951162	29.246817059734997	20.56038012643387
18-19	19.19662057466727	28.81879186722676	32.020806207396326	19.963781350709645
20-21	20.316909499036722	27.87687132142836	31.921974512911454	19.884244666623463
22-23	19.853461024078996	28.924030683485242	31.133751244601815	20.08875704783395
24-25	19.815460163904486	29.27391844838658	31.65294904349773	19.2576723442112
26-27	19.697922619643794	29.18473425910106	31.857535069786074	19.259808051469072
28-29	19.56013267897227	28.245391291027673	32.386895889426334	19.807580140573723
30-31	19.572284116818768	27.91921724119647	32.588830692911806	19.919667949072956
32-33	18.787006651623432	29.022125927191528	32.238280122309746	19.952587298875294
34-35	19.842561552556074	28.63475281766068	31.950917028509483	19.571768601273764
36-37	19.44009120206442	28.636446654451404	32.124572122097646	19.79889002138653
38-39	18.72514478622307	28.55477426310735	32.637289154142096	20.082791796527488
40-41	19.127909717026153	28.04551854972221	33.03276322223728	19.79380851101436
42-43	19.592933597081295	28.05940515310098	32.28818539733788	20.059475852479856
44-45	19.08637389311448	28.843536613386906	32.137386365644865	19.932703127853745
46-47	19.904200718936487	28.050821931024224	31.12246739120028	20.922509958839008
48-49	19.539832028821994	28.259707243499292	31.031511534394028	21.16894919328469
50-51	19.09395599984976	28.29822536802422	31.20692207364682	21.400896558479193
52-53	18.918094890209918	28.61766715959772	31.944878132125158	20.5193598180672
54-55	19.27299052040558	28.67606770633878	30.82576752900143	21.225174244254212
56-57	19.787916904785753	29.147469849705125	29.69818774192408	21.36642550358504
58-59	18.74694372926891	29.175528624368862	31.09449841810373	20.983029228258502
60-61	19.92703245685871	30.074882315165585	29.28680633701166	20.711278890964042
62-63	18.83141463357155	30.6451161530168	29.56216528312114	20.961303930290516
64-65	18.785754685299853	30.29552296842688	29.601712689930654	21.31700965634261
66-67	18.959262488732303	30.453712595664957	29.030816046378728	21.556208869224015
68-69	18.96649116488845	30.336546569835054	29.02252947921707	21.674432786059427
70-71	19.001755698656126	29.75025481196939	29.316117077998978	21.931872411375515
72-73	19.06663701224865	29.22965775659418	29.19224605704253	22.51145917411464
74-75	19.013686201269053	29.270825355116564	28.998412212121387	22.717076231492992
76-77	18.8974742684098	28.991194994491348	29.321419523604717	22.789911213494136
78-79	18.86087266471458	28.806935009691692	29.22722746902488	23.104964856568845
80-81	18.88915237461189	28.870343421727064	28.94185279232677	23.298651411334273
82-83	19.037841786806336	28.626872794329916	28.96379902552833	23.371486393335413
84-85	18.92140891871351	28.318005337795242	28.92123217052665	23.839353572964598
86-87	18.62756505806178	28.42773650380303	29.14798536525013	23.796713072885062
88-89	19.065546376917258	27.99582873971822	28.975382641695074	23.963242241669448
90-91	19.05656167080184	27.745803516260477	29.013088949326992	24.184545863610687
92-93	19.05330725315643	27.646435872812003	28.91070092439302	24.38955594963855
94-95	18.87066746006964	27.208910465260143	29.131415222732173	24.789006851938044
96-97	19.094474851678815	27.02737534834122	28.988543771688473	24.889606028291496
98-99	18.85497509982899	27.070779527802614	28.78197584072139	25.292269531647005
100-101	18.867952319020105	27.001056075082964	29.041401972813063	25.089589633083868
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	154.0
1	98.0
2	53.5
3	60.0
4	45.5
5	48.5
6	76.0
7	153.0
8	267.0
9	357.0
10	352.0
11	354.5
12	424.5
13	516.5
14	587.5
15	657.0
16	763.0
17	851.5
18	912.0
19	1024.0
20	1151.5
21	1325.0
22	1580.5
23	1895.5
24	2323.5
25	2925.0
26	3847.0
27	5252.5
28	6741.5
29	8680.0
30	11248.0
31	13666.5
32	16818.5
33	20276.0
34	23628.5
35	26757.5
36	29915.5
37	32543.0
38	32302.5
39	31327.0
40	30648.0
41	29521.5
42	28808.0
43	29011.5
44	28861.0
45	28567.5
46	27424.0
47	25110.0
48	24150.0
49	23295.0
50	21576.5
51	19516.5
52	17054.0
53	14537.5
54	12085.5
55	9565.5
56	7527.5
57	5772.5
58	4126.0
59	2983.0
60	2183.0
61	1558.0
62	1073.5
63	680.0
64	409.0
65	293.0
66	234.5
67	163.0
68	122.5
69	78.0
70	31.0
71	14.5
72	11.5
73	8.0
74	9.0
75	10.0
76	7.5
77	6.0
78	2.0
79	3.0
80	3.5
81	0.5
82	1.5
83	2.0
84	0.5
85	0.5
86	1.0
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14375519197798897
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	1.4729015571515264E-4
44-45	0.0
46-47	0.0018411269464394078
48-49	8.837409342909156E-4
50-51	3.6822538928788157E-4
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0031667383478757817
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	7.364507785757632E-5
90-91	7.364507785757632E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.003093093270018205
100-101	4.418704671454578E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	678932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.14453854163984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.9472557730015	26.474221510946393
2	15.01194119397537	12.052949036927501
3	6.810537909035247	8.20217704735694
4	3.6442447433894443	5.851860942246637
5	2.168306818277948	4.352283831822977
6	1.42963460146811	3.4435212815459075
7	0.9691392848215731	2.7233954560215836
8	0.684664769455716	2.1988440980414357
9	0.4975608673677671	1.7976916275171368
>10	2.5560642615556257	18.18836802243806
>50	0.19056009003564323	5.366720449427875
>100	0.08712377197439934	5.917694976643963
>500	7.414789104204095E-4	0.1657984508433218
>1k	0.001853697276051024	1.9632382092228526
>5k	3.7073945521020474E-4	1.301235058997453
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT	8743	1.2877578314175793	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4003	0.5896024933277559	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3314	0.4881195760400158	No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT	2381	0.3506978607577784	RNA PCR Primer, Index 31 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1827	0.2690991144915838	No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT	1666	0.24538539942144427	RNA PCR Primer, Index 31 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	1.472901557151526E-4	0.0
16-17	0.0	0.0	0.0	2.2093523357272893E-4	0.0
18-19	0.0	0.0	0.0	2.945803114303052E-4	0.0
20-21	0.0	0.0	0.0	0.001178321245721221	0.0
22-23	0.0	0.0	0.0	0.004050479282166697	0.0
24-25	0.0	0.0	0.0	0.008911054420766734	0.0
26-27	0.0	0.0	0.0	0.018263979308678926	0.0
28-29	0.0	0.0	0.0	0.043966111480973055	0.0
30-31	0.0	0.0	0.0	0.10435507532418564	0.0
32-33	0.0	0.0	0.0	0.22174532942916228	0.0
34-35	0.0	0.0	0.0	0.4089511173431213	0.0
36-37	0.0	0.0	0.0	0.7072136826663054	0.0
38-39	0.0	0.0	0.0	1.0742607507084656	0.0
40-41	0.0	0.0	0.0	1.4388038861034684	0.0
42-43	0.0	0.0	0.0	1.7758774074575951	0.0
44-45	0.0	0.0	0.0	2.1930767735207652	0.0
46-47	0.0	0.0	0.0	2.707561287433793	0.0
48-49	0.0	0.0	0.0	3.209378847955318	0.0
50-51	0.0	0.0	0.0	3.671575356589467	0.0
52-53	0.0	0.0	0.0	4.092162396234086	0.0
54-55	0.0	0.0	0.0	4.564300990379007	0.0
56-57	0.0	0.0	0.0	5.124960968108735	0.0
58-59	0.0	0.0	0.0	5.726052093582274	0.0
60-61	0.0	0.0	0.0	6.306007081710687	0.0
62-63	0.0	0.0	0.0	6.872264085357591	0.0
64-65	0.0	0.0	0.0	7.458994420648901	0.0
66-67	0.0	0.0	0.0	8.111931680934173	0.0
68-69	0.0	0.0	0.0	8.839839630478458	0.0
70-71	0.0	0.0	0.0	9.584023142229267	0.0
72-73	0.0	0.0	0.0	10.31767540784644	0.0
74-75	0.0	0.0	0.0	11.048455515427172	0.0
76-77	0.0	0.0	0.0	11.811271231875946	0.0
78-79	0.0	0.0	0.0	12.674465189444598	0.0
80-81	0.0	0.0	0.0	13.535597084833238	0.0
82-83	0.0	0.0	0.0	14.403430682306919	0.0
84-85	0.0	0.0	0.0	15.285625069962823	0.0
86-87	0.0	0.0	0.0	16.208103315206827	0.0
88-89	0.0	0.0	0.0	17.165931197822463	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1840	0.0	61.2571	1
CTAACGC	25	0.0050209323	56.998455	3
GTATCAA	3570	0.0	41.29709	1
TCAACGC	4465	0.0	32.87145	4
ATCAACG	4470	0.0	32.83468	3
AACGCAG	4515	0.0	32.717827	6
CAACGCA	4515	0.0	32.612625	5
TATCAAC	4620	0.0	31.76862	2
ACGCAGA	5015	0.0	29.45583	7
CGCAGAG	5030	0.0	29.27356	8
GCAGAGT	5390	0.0	26.789625	9
GTACATG	3850	0.0	24.45853	1
ATAGCCC	375	0.0	24.066013	3
GAGTACT	3180	0.0	23.82404	12-13
CAGAGTA	5355	0.0	23.594131	10-11
TACATGG	4050	0.0	23.338873	2
ACATGGG	3890	0.0	23.32199	3
GTACTTT	3295	0.0	22.776318	14-15
CTGTGCG	105	0.002195649	22.618433	9
AGAGTAC	5240	0.0	22.208366	10-11
>>END_MODULE