FastQCFastQC Report
Fri 10 Feb 2017
SRR2031532_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031532_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170028
Sequences flagged as poor quality0
Sequence length101
%GC35

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41322.4301879690403934No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39512.323734914249418No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33611.976733243936293No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27621.6244383278048322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21481.2633213353094785No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA6280.3693509304349872No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.33053379443385794No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.2758369209777213No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4190.24642999976474464No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4140.24348930764344695No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4070.23937233867363022No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2910.17114828145952432No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.15762109770155505No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2660.15644482085303596No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2490.14644646764062388No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.1364481144282118No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2250.13233114545839508No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.12762603806431883No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1910.11233443903357093No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA1800.10586491636671606No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCAT250.005013938557.05
ACATATA405.375155E-447.5000042
GTACCCA459.6019096E-442.2222251
AGTCTGG459.6019096E-442.2222251
TGCTTCG308.27843E-539.58333676-77
GCTTCGC308.27843E-539.58333678-79
ATGTATC500.001611944938.08
GGTATCA22950.037.875821
TTAGACC352.0503032E-433.9285758-59
TGCGAAA352.0503032E-433.9285788-89
GTTCCGT352.0503032E-433.9285730-31
AACTAAG352.0503032E-433.9285794-95
GTACGTG352.0503032E-433.9285734-35
TACGTGT352.0503032E-433.9285736-37
CATATAG600.0039415731.6666683
GACATAT600.0039415731.6666681
ACCCATG600.0039415731.6666683
GGATATG753.0675062E-431.6666649
CTTCGCA404.48706E-429.68750278-79
TGTTCCG404.48706E-429.68750228-29
Produced by FastQC (version 0.11.5)