##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031532_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 170028 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.036605735525914 33.0 31.0 34.0 16.0 34.0 2 30.127626038064317 33.0 31.0 34.0 19.0 34.0 3 30.139177076716777 34.0 31.0 34.0 18.0 34.0 4 33.22711553391206 37.0 35.0 37.0 20.0 37.0 5 33.21584680170325 37.0 35.0 37.0 19.0 37.0 6 33.46095349001341 37.0 35.0 37.0 20.0 37.0 7 33.477421365892674 37.0 35.0 37.0 21.0 37.0 8 33.51497988566589 37.0 35.0 37.0 21.0 37.0 9 35.07598160303009 39.0 37.0 39.0 22.0 39.0 10-11 35.070950078810554 39.0 37.0 39.0 22.0 39.0 12-13 35.016394358576235 39.0 37.0 39.0 20.5 39.0 14-15 36.11398416731362 40.0 37.0 41.0 19.0 41.0 16-17 36.07900169383866 40.0 37.0 41.0 20.0 41.0 18-19 36.034617827651914 40.0 36.5 41.0 19.0 41.0 20-21 36.02070835391818 40.0 36.0 41.0 19.0 41.0 22-23 35.87586750417579 40.0 35.0 41.0 18.5 41.0 24-25 35.72763309572541 40.0 35.0 41.0 18.0 41.0 26-27 35.443521067118354 40.0 35.0 41.0 17.5 41.0 28-29 35.172183405086216 40.0 35.0 41.0 17.0 41.0 30-31 34.67746488813607 39.0 35.0 41.0 16.0 41.0 32-33 34.18993342273038 38.0 35.0 41.0 15.0 41.0 34-35 33.87229162255629 38.0 35.0 41.0 13.0 41.0 36-37 33.51949384807208 37.0 34.0 41.0 12.0 41.0 38-39 33.157279977415484 37.0 33.5 41.0 10.0 41.0 40-41 32.79411920389583 36.0 33.0 41.0 8.5 41.0 42-43 32.366636671607026 35.0 32.5 40.5 8.0 41.0 44-45 31.97922695085515 35.5 30.5 41.0 8.0 41.0 46-47 31.29915660949961 35.0 26.0 41.0 7.5 41.0 48-49 30.651213329569245 35.0 22.5 41.0 7.0 41.0 50-51 29.588388383089843 35.0 19.5 39.5 7.0 40.5 52-53 29.49368045263133 35.0 18.0 39.5 7.0 40.5 54-55 29.81210447690969 35.0 17.5 40.5 7.0 41.0 56-57 29.65457454066389 35.0 15.0 41.0 7.0 41.0 58-59 29.343655162679088 35.0 14.0 41.0 7.0 41.0 60-61 29.074525960430044 35.0 13.0 41.0 7.0 41.0 62-63 28.760180676123934 35.0 12.5 40.0 7.0 41.0 64-65 28.431511280494977 35.0 11.0 40.0 7.0 41.0 66-67 28.082727550756346 35.0 10.0 39.5 7.0 41.0 68-69 27.682096478227116 35.0 10.0 39.0 7.0 41.0 70-71 27.217375961606322 35.0 10.0 39.0 7.0 41.0 72-73 26.779903898181473 35.0 10.0 37.5 7.0 41.0 74-75 26.352662502646623 35.0 10.0 37.0 7.0 40.0 76-77 25.95288717152469 35.0 8.0 36.5 7.0 39.0 78-79 25.536743948055616 34.0 8.0 36.0 7.0 39.0 80-81 25.16981614792858 34.0 8.0 36.0 7.0 38.5 82-83 24.80284423741972 33.5 8.0 35.0 7.0 37.0 84-85 24.424615357470536 33.0 7.5 35.0 7.0 37.0 86-87 24.11337544404451 33.0 7.0 35.0 7.0 36.5 88-89 23.81248088550121 32.5 7.0 35.0 7.0 36.0 90-91 23.528195356059 32.0 7.0 35.0 7.0 36.0 92-93 23.257739901663257 31.5 7.0 35.0 7.0 36.0 94-95 23.017579457501117 31.0 7.0 35.0 6.0 35.0 96-97 22.801379772743314 30.5 7.0 35.0 5.0 35.0 98-99 22.586209330227963 29.5 7.0 35.0 5.0 35.0 100-101 21.54539252358435 26.5 7.0 34.5 5.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.756778210575586 1101 2 0.5383287581666565 1101 3 0.7519617438368726 1101 4 0.7312126959978826 1101 5 0.45893038064470915 1101 6 0.29542311202045823 1101 7 0.5081845898242321 1101 8 0.5061148174857095 1101 9 1.123459064641274 1101 10-11 0.9353515964528327 1101 12-13 0.9492095514334196 1101 14-15 1.0257249165370652 1101 16-17 1.6648218826845707 1101 18-19 1.1582624560649961 1101 20-21 0.8693562601429434 1101 22-23 1.1042253290602275 1101 24-25 0.8703187655477578 1101 26-27 0.8934067815787188 1101 28-29 0.9163803463424856 1101 30-31 1.4195871218846605 1101 32-33 0.8791382783442145 1101 34-35 0.5929896268677908 1101 36-37 0.5460166472225438 1101 38-39 0.8842976916273528 1101 40-41 0.6353111065759904 1101 42-43 0.3609762103983414 1101 44-45 0.6124687927573476 1101 46-47 0.7908169788963448 1101 48-49 1.3454342551688505 1101 50-51 1.3022082605008016 1101 52-53 0.9830583963052462 1101 54-55 0.8577491478809662 1101 56-57 0.4818593034234304 1101 58-59 0.5258312477034401 1101 60-61 0.15250597262201282 1101 62-63 -0.284734842061372 1101 64-65 -0.7728643700097955 1101 66-67 -0.8135436828025995 1101 68-69 -0.3542863324630261 1101 70-71 -0.5410374997596037 1101 72-73 -1.011449955999705 1101 74-75 -1.0071381438609883 1101 76-77 -0.6426751648417977 1101 78-79 -0.5320626894720064 1101 80-81 -0.8087151842500333 1101 82-83 -1.3561931543766192 1101 84-85 -0.5999000942474453 1101 86-87 -0.5720605219425252 1101 88-89 -0.944538933516899 1101 90-91 -0.9195463018246386 1101 92-93 -0.7565857565179961 1101 94-95 -1.1140048747175975 1101 96-97 -0.8902188110493796 1101 98-99 -0.8926584905529928 1101 100-101 -1.1627552400058399 1102 1 -0.1611705073731322 1102 2 -0.4950045751666785 1102 3 -0.3871408202656923 1102 4 -0.5393001245149378 1102 5 0.014058585772914967 1102 6 -0.6270127854154453 1102 7 -0.3482256665860177 1102 8 -0.19837236200147146 1102 9 0.25038214156435146 1102 10-11 0.29977467337591435 1102 12-13 0.4194018591257276 1102 14-15 0.25296850628065215 1102 16-17 0.07411675447944077 1102 18-19 0.20185219965474488 1102 20-21 0.081856260142942 1102 22-23 0.6295458418807414 1102 24-25 0.42672902195801043 1102 26-27 0.6248170379889757 1102 28-29 0.35804701300914843 1102 30-31 0.3420230193205498 1102 32-33 0.6121510988570336 1102 34-35 0.24331013968830462 1102 36-37 0.4299910061969001 1102 38-39 -0.0993561545264896 1102 40-41 -0.08680427803939494 1102 42-43 -0.05857507165293896 1102 44-45 -0.27535172006316344 1102 46-47 0.07286826094762233 1102 48-49 -0.2577708730362822 1102 50-51 -0.15644558565304578 1102 52-53 -0.09290314215629536 1102 54-55 0.5029414555732714 1102 56-57 -0.15564069657656887 1102 58-59 -1.0357072138350212 1102 60-61 -0.5923658222497785 1102 62-63 -0.47159381642034504 1102 64-65 -0.5260694982149197 1102 66-67 -0.5465565033154185 1102 68-69 -0.8885812042578998 1102 70-71 -1.1169990382211452 1102 72-73 -0.681321750871497 1102 74-75 -0.5891894259122701 1102 76-77 -0.4282520879187217 1102 78-79 -0.1596267920361072 1102 80-81 -0.31224082527567276 1102 82-83 -0.18792392360738575 1102 84-85 -0.07201547886283066 1102 86-87 -0.16148359886560115 1102 88-89 -0.47883380531177266 1102 90-91 -0.32627707105540793 1102 92-93 -0.293444730876967 1102 94-95 0.155866920154196 1102 96-97 -0.299834195664765 1102 98-99 -0.2243892597837629 1102 100-101 -0.12140908615968726 1103 1 -0.23977747254726012 1103 2 -0.6295816895945379 1103 3 -0.24373286006668593 1103 4 -0.5439021145646876 1103 5 -0.782458827162408 1103 6 -0.3215401485995244 1103 7 -0.7115341242974651 1103 8 -0.933322610757692 1103 9 -0.8835730823162393 1103 10-11 -0.8580611475196065 1103 12-13 -0.9651130662474046 1103 14-15 -0.5297429365054214 1103 16-17 -0.5747016534807656 1103 18-19 -0.923396556564164 1103 20-21 -0.5149720980660106 1103 22-23 -0.8490611232933887 1103 24-25 -0.9547386397335274 1103 26-27 -0.883143161016001 1103 28-29 -0.23007487753810807 1103 30-31 -0.38042722943566787 1103 32-33 -0.08921706034694665 1103 34-35 -0.2894013030977689 1103 36-37 -0.28219804852946595 1103 38-39 -0.24450540825783662 1103 40-41 0.03241213987104885 1103 42-43 -0.2851919870758266 1103 44-45 -0.37734176981440726 1103 46-47 -0.5518207937787452 1103 48-49 -0.43513405711588504 1103 50-51 -0.5010351378918507 1103 52-53 -0.7447688137980855 1103 54-55 -0.5117973503968756 1103 56-57 -0.6785884577705978 1103 58-59 -0.3013788556260657 1103 60-61 0.28145258571041865 1103 62-63 0.6976847905448302 1103 64-65 -0.08141775692138964 1103 66-67 -0.16608386649949836 1103 68-69 -0.22763593062605736 1103 70-71 0.005389021480347367 1103 72-73 -0.47205558171726736 1103 74-75 -0.6411794756635096 1103 76-77 -0.4174311923963323 1103 78-79 -0.5027859960162075 1103 80-81 -0.45875823821099715 1103 82-83 0.011702942064253818 1103 84-85 0.13395467039089937 1103 86-87 0.4172477444179812 1103 88-89 0.26793236384245844 1103 90-91 0.2757751677505631 1103 92-93 -0.19580791495657124 1103 94-95 -0.11328730870152626 1103 96-97 0.05818819239493678 1103 98-99 -0.054613140380777025 1103 100-101 -0.127379235413418 1104 1 -0.32636658580450373 1104 2 -0.3366712418333435 1104 3 0.1461925130676427 1104 4 0.0038371303870263773 1104 5 -0.39231396324669277 1104 6 -0.4500520011017173 1104 7 -1.0251864508997457 1104 8 -0.3841566757269632 1104 9 0.26655861215258625 1104 10-11 0.29903937925826085 1104 12-13 0.3304312708904362 1104 14-15 0.41203713373163 1104 16-17 0.33563636232257466 1104 18-19 0.04033259181160531 1104 20-21 -0.20784962220999859 1104 22-23 0.2665556458023062 1104 24-25 0.33383686509527166 1104 26-27 0.41942488112622556 1104 28-29 0.7104979934013116 1104 30-31 0.9849151761832999 1104 32-33 0.49254325571978086 1104 34-35 0.07884935537457949 1104 36-37 0.028765516000831326 1104 38-39 0.29549678664998424 1104 40-41 0.4087839572547196 1104 42-43 0.3301504185431412 1104 44-45 0.34352082895644287 1104 46-47 0.9025251236927261 1104 48-49 0.942719323042148 1104 50-51 0.8714955908175419 1104 52-53 0.8563615637260611 1104 54-55 0.7316179261615083 1104 56-57 -0.07696422598833408 1104 58-59 0.11943984498850924 1104 60-61 0.07699692284825943 1104 62-63 -0.6034565615183816 1104 64-65 -0.5836675374306068 1104 66-67 -0.5740074837075753 1104 68-69 -0.6868655179833887 1104 70-71 -0.24126374410349882 1104 72-73 -0.6715178293028714 1104 74-75 -0.6850227592456015 1104 76-77 -0.926046205565779 1104 78-79 -0.7792346351733599 1104 80-81 -0.4950839625305754 1104 82-83 -0.34233568831326977 1104 84-85 -0.26760371415694806 1104 86-87 -0.6710424223950149 1104 88-89 -0.6999122366843231 1104 90-91 -0.7586300122318796 1104 92-93 -1.0936898289161867 1104 94-95 -0.7590840602379636 1104 96-97 -0.6351283133118244 1104 98-99 -0.7648304362543499 1104 100-101 -0.74787967439498 1105 1 0.5854961592935375 1105 2 0.6616620914999913 1105 3 0.7578591797343108 1105 4 0.7756998754850599 1105 5 0.9807252524395835 1105 6 0.9263205479178893 1105 7 0.486774333413976 1105 8 0.8099609713318614 1105 9 0.9587154748976872 1105 10-11 0.8347746733759109 1105 12-13 0.9785685257923902 1105 14-15 0.914635172947321 1105 16-17 0.8516167544794371 1105 18-19 0.8101855329880792 1105 20-21 0.8660229268096131 1105 22-23 0.952879175214072 1105 24-25 0.8383956886246793 1105 26-27 0.6448170379889717 1105 28-29 0.47888034634248555 1105 30-31 0.6261896859872209 1105 32-33 0.6104844321903684 1105 34-35 0.6016434730216318 1105 36-37 0.3099910061969027 1105 38-39 0.8623105121401835 1105 40-41 0.5523623886272659 1105 42-43 0.6347582616803962 1105 44-45 0.5479816132701671 1105 46-47 0.7270349276142909 1105 48-49 0.5855624602970515 1105 50-51 0.35438774768028836 1105 52-53 0.3487635245103746 1105 54-55 0.4971081222399363 1105 56-57 0.3118593034234287 1105 58-59 0.5676261194983141 1105 60-61 0.10180084441688919 1105 62-63 0.3300728502463208 1105 64-65 -0.04773616488158794 1105 66-67 0.01844349668457923 1105 68-69 -0.33191453759123135 1105 70-71 0.08633429511218793 1105 72-73 0.24451158246183624 1105 74-75 0.31747724075439976 1105 76-77 -0.156585421252057 1105 78-79 -0.3779601253694409 1105 80-81 -0.20557415860900363 1105 82-83 -0.512923923607385 1105 84-85 -0.2653488121961658 1105 86-87 -0.006483598865603568 1105 88-89 -0.16216713864510623 1105 90-91 -0.4037770710554085 1105 92-93 0.2482219357896973 1105 94-95 -0.12746641317913898 1105 96-97 0.19933247100190243 1105 98-99 0.3139440735495711 1105 100-101 0.3819242471736466 1106 1 0.7208470364865178 1106 2 1.1304340213245503 1106 3 0.6461925130676427 1106 4 1.3076297000464692 1106 5 0.9710761296325643 1106 6 0.6238644075670123 1106 7 0.7430024035894149 1106 8 0.9428557081739655 1106 9 0.8977505626169844 1106 10-11 0.860958883902228 1106 12-13 1.049665017020466 1106 14-15 0.7020913132981974 1106 16-17 0.4203886843039939 1106 18-19 0.6924223750933365 1106 20-21 0.7213299443534638 1106 22-23 0.8155107541614441 1106 24-25 0.8328693728351979 1106 26-27 0.7770100204451111 1106 28-29 0.4657224516056431 1106 30-31 0.5023300368644144 1106 32-33 0.901405484821943 1106 34-35 1.0838803151268976 1106 36-37 1.5902980237407647 1106 38-39 1.669722792841938 1106 40-41 1.4085904588027063 1106 42-43 2.002170542382153 1106 44-45 1.7794728413403433 1106 46-47 1.057517383754643 1106 48-49 1.5582378988935481 1106 50-51 1.038291256452215 1106 52-53 0.8985442262647574 1106 54-55 1.1621519818890604 1106 56-57 0.9719908823708003 1106 58-59 1.5379769966912953 1106 60-61 1.5067569847677653 1106 62-63 1.4766517976147426 1106 64-65 1.393886642135957 1106 66-67 1.1312943738775623 1106 68-69 0.7355416027596462 1106 70-71 0.27247464598938365 1106 72-73 0.04345895088288998 1106 74-75 -0.1948911802982316 1106 76-77 -0.24667314055029976 1106 78-79 -0.03813556396593043 1106 80-81 -0.1196969656265523 1106 82-83 -0.319502870975807 1106 84-85 0.03851083692664048 1106 86-87 0.08127955902913442 1106 88-89 -0.4073425772415966 1106 90-91 -0.20193496579225112 1106 92-93 -0.11033069578924781 1106 94-95 0.308059902610335 1106 96-97 0.6297710674931274 1106 98-99 0.5979791612688672 1106 100-101 -0.44969855984389895 1107 1 0.381966747528832 1107 2 0.4419235294084878 1107 3 0.09145395097613829 1107 4 0.4866802676419226 1107 5 0.3094834223742211 1107 6 0.32200682242769574 1107 7 0.3947481896231295 1107 8 0.1419871151227099 1107 9 0.3286501154205652 1107 10-11 0.35091846422558604 1107 12-13 -0.0135229774755814 1107 14-15 0.36587700301267745 1107 16-17 0.5505056433683322 1107 18-19 0.46802867024297967 1107 20-21 0.34974841700569215 1107 22-23 0.04882688763238008 1107 24-25 0.14551987163121538 1107 26-27 0.48723533864256297 1107 28-29 0.49031825483921665 1107 30-31 0.17690864023558106 1107 32-33 0.11917724264788632 1107 34-35 -0.14214737665156463 1107 36-37 -0.30334232713642706 1107 38-39 0.2419837147545536 1107 40-41 -0.31874872248383923 1107 42-43 0.1790066276934681 1107 44-45 -0.17404453052068192 1107 46-47 -0.020677490686363598 1107 48-49 -0.329339500487265 1107 50-51 0.10556421826852258 1107 52-53 -0.1571188284308036 1107 54-55 -0.4889703091326112 1107 56-57 0.009637081201208986 1107 58-59 0.18520781884472015 1107 60-61 0.032062282325380664 1107 62-63 0.19392905939664828 1107 64-65 0.6397801749876919 1107 66-67 0.6098160457041892 1107 68-69 0.05945801142837581 1107 70-71 0.27875259576578415 1107 72-73 0.4432697523964748 1107 74-75 0.8823792015387113 1107 76-77 0.6797544480289908 1107 78-79 0.5140660184214099 1107 80-81 1.1119421812602752 1107 82-83 0.7981871875037285 1107 84-85 0.6988995538169078 1107 86-87 1.0115556168206723 1107 88-89 0.995349201224176 1107 90-91 0.6780529943040676 1107 92-93 1.0325356612798906 1107 94-95 0.9627296652522368 1107 96-97 1.2345612291718346 1107 98-99 0.7339440735495693 1107 100-101 1.000486338676918 1108 1 0.01850428937483528 1108 2 -0.10801270524797602 1108 3 -0.5214904137616259 1108 4 -0.4378773602872954 1108 5 -0.3501690565035034 1108 6 -0.5847363626512134 1108 7 -0.7167216015453732 1108 8 -0.4254048823266743 1108 9 -0.36364224867954675 1108 10-11 -0.5532741071118892 1108 12-13 -0.29187862867915015 1108 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2309.0 13 2032.0 14 2069.0 15 2270.0 16 2563.0 17 2964.0 18 3430.0 19 4257.0 20 5449.0 21 6059.0 22 4979.0 23 3842.0 24 3417.0 25 2804.0 26 2534.0 27 2625.0 28 2664.0 29 2513.0 30 2377.0 31 2669.0 32 3051.0 33 3792.0 34 5148.0 35 10502.0 36 11028.0 37 17388.0 38 32478.0 39 12858.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.31387771425882 23.566706660079518 16.479050509327877 15.64036511633378 2 15.442750605782576 26.21744653821724 40.92678852894817 17.413014327052014 3 16.605500270543676 36.08405674359517 32.76048650810455 14.549956477756604 4 11.312254452207872 24.05192086009363 38.75008822076364 25.885736466934855 5 9.253769967299503 42.37949043686922 33.88089020631896 14.485849389512314 6 20.08904415743289 41.91192038958289 21.001246853459428 16.997788599524785 7 19.67440656832992 41.65725645187852 24.482438186651613 14.185898793139954 8 23.957230573787847 34.762509704284 22.455125038229 18.825134683699154 9 27.879525725174677 18.724563013150775 22.432775778107136 30.96313548356741 10-11 21.2538523066789 32.3361446350013 27.213458959700752 19.19654409861905 12-13 24.827969510904087 33.03720563671866 27.608393911591033 14.526430940786222 14-15 22.536288140776815 32.05883736796292 29.81832404074623 15.586550450514034 16-17 15.963547180464394 35.630307949278944 30.647011080527914 17.75913378972875 18-19 15.817688851248029 31.654198132072363 36.91215564495259 15.61595737172701 20-21 17.587397369844968 31.00312889641706 37.21916390241608 14.190309831321901 22-23 17.542110711176985 31.770943609287883 35.87026842637683 14.816677253158304 24-25 16.23056202507822 33.37038605406168 35.53267697085186 14.866374950008234 26-27 15.728291810760581 33.0169148610817 36.02494883195709 15.229844496200625 28-29 15.567141882513468 32.984273178535304 37.10918201707954 14.339402921871692 30-31 16.084703695861858 33.52241983673277 36.354306349542426 14.038570117862939 32-33 14.827851883219234 34.27464888136072 36.75571082409956 14.141788411320489 34-35 14.471439998117958 34.49078975227609 36.06700072929165 14.9707695203143 36-37 15.330710235961137 34.06056649493024 35.54855670830687 15.060166560801749 38-39 15.072517467711199 33.9652880702002 35.79351636201096 15.168678100077635 40-41 14.624944126849696 33.97175759286706 36.53133601524455 14.871962265038698 42-43 14.78579998588468 33.88147834474322 35.918789846378246 15.41393182299386 44-45 14.891076763827135 33.499482438186654 35.5982544051568 16.011186392829416 46-47 15.0616369068624 33.644164490554495 34.878078904651 16.416119697932103 48-49 15.091337897287504 33.46095937139765 34.534606064883434 16.91309666643141 50-51 14.844613828310631 33.22099889429976 34.21554096972264 17.718846307666972 52-53 14.771978732914578 33.39185310654716 34.03880537323264 17.79736278730562 54-55 15.180734937774954 33.18071141223798 32.98515538617169 18.653398263815372 56-57 15.199555367351259 32.94398569647352 32.5969840261604 19.25947491001482 58-59 15.044874961771 31.904156962382668 33.03985225962783 20.011115816218506 60-61 15.454219305055638 31.39041804879196 33.09807796362952 20.057284682522877 62-63 15.271602324323052 30.859623120897734 33.88941821347073 19.979356341308492 64-65 15.61272261039358 30.60260662949632 33.93205824922954 19.85261251088056 66-67 15.937375020584845 30.915496271202393 32.93604582774602 20.211082880466748 68-69 16.053532359376103 30.85227139059449 33.127484884842495 19.96671136518691 70-71 16.05970781283083 30.82462888465429 33.37891406121345 19.736749241301432 72-73 16.593737502058485 30.114451737360902 33.12483826193333 20.166972498647283 74-75 16.634613122544522 30.183558002211402 32.82841649610652 20.353412379137552 76-77 16.82399369515609 29.961535747053425 33.269226245089044 19.945244312701437 78-79 17.297739195897147 29.730985484743687 32.64197661561625 20.32929870374291 80-81 17.59680758463312 30.151210388877125 31.97943868068789 20.272543345801868 82-83 18.181711247559225 29.98976639141788 31.536276378008328 20.292245983014563 84-85 18.168772202225515 29.899781212506177 30.769343872773895 21.162102712494413 86-87 18.501364481144282 29.984473145599548 30.53614698755499 20.97801538570118 88-89 19.707930458512717 29.377220222551582 30.0571082409956 20.857741077940105 90-91 20.53485308302162 29.305467334791917 29.378984637824356 20.780694944362104 92-93 21.310607664619948 28.777907168231113 29.151669136848046 20.759816030300893 94-95 21.555273249111913 28.200355235608253 28.875538146658197 21.36883336862164 96-97 22.41807231750065 27.965393935116566 28.22799774154845 21.388536005834332 98-99 22.74390100454043 28.050379937422072 27.97627449596537 21.229444562072132 100-101 24.397158115133976 27.327851883219235 27.562813183710915 20.71217681793587 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3307.0 1 2016.0 2 728.0 3 695.0 4 520.5 5 472.0 6 773.0 7 1302.0 8 1837.5 9 1919.5 10 1386.0 11 945.0 12 940.5 13 916.0 14 797.5 15 676.5 16 611.0 17 582.0 18 556.5 19 618.5 20 697.5 21 747.5 22 901.0 23 1098.5 24 1259.0 25 1541.0 26 1981.0 27 2494.0 28 2983.0 29 3647.5 30 4411.0 31 5023.5 32 5573.0 33 6194.0 34 6751.0 35 7017.5 36 7645.5 37 8328.5 38 8015.5 39 7156.5 40 6140.5 41 5169.0 42 4600.0 43 4476.5 44 4475.5 45 4369.5 46 3962.0 47 3424.0 48 3349.0 49 3293.0 50 3241.0 51 3099.0 52 2486.5 53 1942.5 54 1549.5 55 1254.0 56 1116.5 57 948.5 58 861.5 59 797.0 60 700.0 61 702.0 62 696.0 63 586.0 64 475.5 65 397.5 66 335.5 67 295.0 68 242.0 69 226.5 70 223.0 71 192.5 72 168.0 73 137.0 74 115.5 75 103.0 76 85.5 77 69.5 78 48.0 79 42.0 80 38.5 81 33.5 82 30.5 83 25.0 84 20.0 85 14.5 86 12.5 87 12.0 88 8.0 89 4.5 90 6.5 91 7.0 92 5.0 93 2.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 170028.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.21053003034795 #Duplication Level Percentage of deduplicated Percentage of total 1 81.68912481284877 35.29830380878444 2 6.367224717571799 5.502623097372197 3 3.077446576834082 3.9893429317524176 4 1.934122771199129 3.3429788034911896 5 1.3311555737035523 2.87599689462912 6 0.9418810398802232 2.441950737525584 7 0.7404382741254935 2.239631119580304 8 0.5648564039744114 1.9526195685416519 9 0.41377432965836397 1.6091467287740844 >10 2.7208384374574655 21.50704589832263 >50 0.15516537362188648 4.702754840379232 >100 0.054443990744521574 4.2193050556378955 >500 0.0027221995372260785 0.6998847248688451 >1k 0.006805498843065197 9.618415790340414 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4132 2.4301879690403934 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3951 2.323734914249418 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3361 1.976733243936293 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2762 1.6244383278048322 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2148 1.2633213353094785 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 628 0.3693509304349872 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 562 0.33053379443385794 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 469 0.2758369209777213 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 419 0.24642999976474464 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 414 0.24348930764344695 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 407 0.23937233867363022 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 291 0.17114828145952432 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 268 0.15762109770155505 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 266 0.15644482085303596 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 249 0.14644646764062388 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 232 0.1364481144282118 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 225 0.13233114545839508 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 217 0.12762603806431883 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 191 0.11233443903357093 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 180 0.10586491636671606 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.9406921212976687E-4 0.0 12-13 0.0 0.0 0.0 5.881384242595337E-4 0.0 14-15 0.0 0.0 0.0 0.0014703460606488345 0.0 16-17 0.0 0.0 0.0 0.004999176606206037 0.0 18-19 0.0 0.0 0.0 0.007351730303244172 0.0 20-21 0.0 0.0 0.0 0.011762768485190676 0.0 22-23 0.0 0.0 0.0 0.03499423624344226 0.0 24-25 0.0 0.0 0.0 0.09263180182087656 0.0 26-27 0.0 0.0 0.0 0.1799703578234173 0.0 28-29 0.0 0.0 0.0 0.2999505963723622 0.0 30-31 0.0 0.0 0.0 0.4908015150445809 0.0 32-33 0.0 0.0 0.0 0.7369374455971958 0.0 34-35 0.0 0.0 0.0 1.1095231373656105 0.0 36-37 0.0 0.0 0.0 1.6764885783518009 0.0 38-39 0.0 0.0 0.0 2.2510998188533655 0.0 40-41 0.0 0.0 0.0 2.7777777777777777 0.0 42-43 0.0 0.0 0.0 3.1665372762133295 0.0 44-45 0.0 0.0 0.0 3.5435340061636906 0.0 46-47 0.0 0.0 0.0 3.971992848236761 0.0 48-49 0.0 0.0 0.0 4.40251017479474 0.0 50-51 0.0 0.0 0.0 4.76803820547204 0.0 52-53 0.0 0.0 0.0 5.088573646693486 0.0 54-55 0.0 0.0 0.0 5.451160985249489 0.0 56-57 0.0 0.0 0.0 5.91490813277813 0.0 58-59 0.0 0.0 0.0 6.333074552426659 0.0 60-61 0.0 0.0 0.0 6.712717905286188 0.0 62-63 0.0 0.0 0.0 7.055602606629496 0.0 64-65 0.0 0.0 0.0 7.449949420095514 0.0 66-67 0.0 0.0 0.0 7.8913473075022935 0.0 68-69 0.0 0.0 0.0 8.375385230667892 0.0 70-71 0.0 0.0 0.0 8.850895146681722 0.0 72-73 0.0 0.0 0.0 9.301409179664525 0.0 74-75 0.0 0.0 0.0 9.784270825981602 0.0 76-77 0.0 0.0 0.0 10.294480909026749 0.0 78-79 0.0 0.0 0.0 10.836450466981908 0.0 80-81 0.0 0.0 0.0 11.381654786270497 0.0 82-83 0.0 0.0 0.0 11.902451360952313 0.0 84-85 0.0 0.0 0.0 12.436186980967841 0.0 86-87 0.0 0.0 0.0 13.005799044863199 0.0 88-89 0.0 0.0 0.0 13.592173053849955 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGCAT 25 0.0050139385 57.0 5 ACATATA 40 5.375155E-4 47.500004 2 GTACCCA 45 9.6019096E-4 42.222225 1 AGTCTGG 45 9.6019096E-4 42.222225 1 TGCTTCG 30 8.27843E-5 39.583336 76-77 GCTTCGC 30 8.27843E-5 39.583336 78-79 ATGTATC 50 0.0016119449 38.0 8 GGTATCA 2295 0.0 37.87582 1 TTAGACC 35 2.0503032E-4 33.92857 58-59 TGCGAAA 35 2.0503032E-4 33.92857 88-89 GTTCCGT 35 2.0503032E-4 33.92857 30-31 AACTAAG 35 2.0503032E-4 33.92857 94-95 GTACGTG 35 2.0503032E-4 33.92857 34-35 TACGTGT 35 2.0503032E-4 33.92857 36-37 CATATAG 60 0.00394157 31.666668 3 GACATAT 60 0.00394157 31.666668 1 ACCCATG 60 0.00394157 31.666668 3 GGATATG 75 3.0675062E-4 31.666664 9 CTTCGCA 40 4.48706E-4 29.687502 78-79 TGTTCCG 40 4.48706E-4 29.687502 28-29 >>END_MODULE