FastQCFastQC Report
Fri 10 Feb 2017
SRR2031532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170028
Sequences flagged as poor quality0
Sequence length101
%GC35

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT98545.795516032653445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43902.581927682499353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39172.3037382078245936No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35692.0990660361822755No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31131.8308749147199284No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT30301.782059425506387RNA PCR Primer, Index 39 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24461.4385865857388194No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT21361.256263674218364RNA PCR Primer, Index 39 (96% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG20621.2127414308231586RNA PCR Primer, Index 39 (96% over 30bp)
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT7890.46404121674077214RNA PCR Primer, Index 39 (96% over 28bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA6710.3946408826781472RNA PCR Primer, Index 39 (96% over 30bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA6570.38640694473851367No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6160.36229326934387274No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5780.3399440092220105No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.28877596631143104No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4330.2546639377043781No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTG4060.2387842002493707Illumina PCR Primer Index 11 (96% over 25bp)
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC3760.22114004752158467No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3740.2199637706730656No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.17879408097489824No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2700.15879737455007412No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC2690.15820923612581458RNA PCR Primer, Index 39 (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2680.15762109770155505No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGC2470.14527019079210482Illumina PCR Primer Index 11 (96% over 26bp)
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.1429176370950667No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT2360.13880066812524994RNA PCR Primer, Index 39 (95% over 22bp)
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.1364481144282118No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC2310.13585997600395228No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2170.12762603806431883No Hit
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2120.12468534594302115No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1920.11292257745783046No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCATG250.00501479956.9975287
GGCATGG250.00501479956.9975288
CTCCTCG352.781769E-454.283369
CTTTACA802.212073E-741.6464421
TATGGGA500.001612288337.9983524
GTATATG1152.4265319E-937.248991
ATATGGG905.6618956E-736.9428443
GGTATCA23700.035.7471541
CGCAGAA851.6245505E-533.5279589
TATATGG1151.0279655E-733.0420462
AGGGTAC300.003942229331.67461268-69
ATGGGAT753.068286E-431.6652935
GGTACAT300.00394791231.66529370-71
GAACGCA300.00394791231.66529354-55
GTATCAA35150.030.737631
AACGCAG36550.029.4994076
GGGAGGA650.005831492629.2295049
ACTCCTC650.005831492629.2295048
TATACAC34950.028.4036333
CTTATAC35500.028.155341
Produced by FastQC (version 0.11.5)