FastQCFastQC Report
Fri 10 Feb 2017
SRR2031531_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031531_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481101
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35410.23907890143886204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29310.1978933239529242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15180.10249132233385838No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20950.054.868731
GTATCAA38550.043.2490271
TCAACGC47250.035.58734
ATCAACG47600.035.325633
CAACGCA47750.035.1151855
AACGCAG48200.034.7873466
TATCAAC49100.034.2464372
ACGCAGA55100.030.4310367
CGCAGAG55550.030.1845198
GTACATG45850.027.3500541
TACATGG44550.027.2951742
GCAGAGT64200.026.11769
ACATGGG45800.025.3056773
GAGTACT37700.022.86803812-13
CAGAGTA59550.022.25440810-11
AGAGTAC55500.022.25225410-11
CATGGGG27650.022.160944
GTACCGT1150.003723777420.6521766
GTATAGA6300.020.3571431
GTACTTT42200.020.03554514-15
Produced by FastQC (version 0.11.5)