FastQCFastQC Report
Fri 10 Feb 2017
SRR2031531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1481101
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42640.2878939383607195No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT38110.2573085832769001No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36030.2432649765275967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19600.1323339866761281No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.057.589861
GTATCAA40550.041.5240331
TCAACGC49100.034.2458044
TAGATCG702.0538399E-433.9279445
ATCAACG49950.033.6630443
CAACGCA50600.033.2306145
AACGCAG52000.032.518636
TATCAAC52400.032.2703932
ACGCAGA59300.028.3552957
CGCAGAG59850.028.0153548
GTACATG45550.025.1660921
TACATGG45300.024.6408232
GCAGAGT70000.023.7495619
ACATGGG46500.023.2898923
GTATAGG3100.023.0153031
CATGGGG27400.022.7094354
GAGTACT43250.022.07473612-13
CAGAGTA65900.021.1187310-11
AGAGTAC61250.020.97716310-11
TATACAG9400.020.2123935
Produced by FastQC (version 0.11.5)