FastQCFastQC Report
Fri 10 Feb 2017
SRR2031530_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031530_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575569
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38700.24562554861132707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27310.17333420497610705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16400.10408937977327555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.050.1794931
GTATCAA38800.047.6224171
TCAACGC46300.039.1900634
CAACGCA47000.038.707455
ATCAACG47000.038.707453
AACGCAG48650.037.4922946
TATCAAC50050.036.918082
GCGTTAG550.00257991534.5454521
ACGCAGA55700.032.661587
CGCAGAG56350.032.031948
GCAGAGT63600.028.0817629
GTACATG45800.026.5502171
TACATGG45100.026.43572
ACATGGG45000.024.911113
AGAGTAC57300.023.0039310-11
CATGGGG27850.022.1723524
CAGAGTA60550.021.8868710-11
GAGTACT41200.021.21359312-13
GTACTTT42600.020.34917814-15
AGTACTT43950.019.83219512-13
Produced by FastQC (version 0.11.5)