##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031530_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1575569 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.13653797453492 34.0 31.0 34.0 30.0 34.0 2 32.2983880744036 34.0 31.0 34.0 30.0 34.0 3 32.40266024528282 34.0 31.0 34.0 30.0 34.0 4 35.63474655822753 37.0 35.0 37.0 33.0 37.0 5 35.620782714054414 37.0 35.0 37.0 33.0 37.0 6 35.744605282282144 37.0 37.0 37.0 35.0 37.0 7 35.7686962614776 37.0 37.0 37.0 35.0 37.0 8 35.81463077783328 37.0 37.0 37.0 35.0 37.0 9 37.62977946380006 39.0 39.0 39.0 35.0 39.0 10-11 37.62796361187609 39.0 38.5 39.0 35.0 39.0 12-13 37.59291119589177 39.0 38.0 39.0 35.0 39.0 14-15 39.0862355123768 41.0 39.0 41.0 36.0 41.0 16-17 39.098190875804235 41.0 39.0 41.0 36.0 41.0 18-19 39.05466183962746 41.0 39.0 41.0 36.0 41.0 20-21 39.01453665310754 41.0 39.0 41.0 36.0 41.0 22-23 38.93947234300751 41.0 39.0 41.0 35.5 41.0 24-25 38.88399587704505 41.0 39.0 41.0 35.0 41.0 26-27 38.777521644561425 41.0 39.0 41.0 35.0 41.0 28-29 38.66887835442307 40.5 39.0 41.0 34.5 41.0 30-31 38.50470655363237 40.0 38.0 41.0 34.0 41.0 32-33 38.392516290939966 40.0 38.0 41.0 34.0 41.0 34-35 38.25231551268145 40.0 38.0 41.0 34.0 41.0 36-37 38.11688729595467 40.0 38.0 41.0 33.0 41.0 38-39 37.968259086082554 40.0 38.0 41.0 33.0 41.0 40-41 37.83229265109938 40.0 38.0 41.0 33.0 41.0 42-43 37.67652099019465 40.0 38.0 41.0 32.5 41.0 44-45 37.85677650423435 40.0 38.0 41.0 33.0 41.0 46-47 37.728669769461064 40.0 38.0 41.0 32.5 41.0 48-49 37.59177922388673 40.0 38.0 41.0 32.0 41.0 50-51 36.538382958791395 39.0 36.5 40.0 30.0 40.5 52-53 36.60855538538776 39.0 36.5 40.0 30.5 40.5 54-55 37.40099322847809 40.0 37.0 41.0 31.5 41.0 56-57 37.4458671756045 40.0 37.0 41.0 32.0 41.0 58-59 37.252712194769 40.0 37.0 41.0 31.0 41.0 60-61 37.083091568823704 40.0 36.0 41.0 31.0 41.0 62-63 36.82690824711581 39.5 36.0 41.0 31.0 41.0 64-65 36.54970648698978 39.0 35.0 41.0 30.0 41.0 66-67 36.265558030146565 39.0 35.0 41.0 30.0 41.0 68-69 35.9560136687127 38.0 35.0 40.5 29.5 41.0 70-71 35.59417740511523 37.0 35.0 40.0 29.0 41.0 72-73 35.225822226763796 37.0 35.0 39.0 29.0 41.0 74-75 34.837060769791734 36.0 35.0 39.0 29.0 41.0 76-77 34.45927852096607 36.0 34.5 38.5 28.5 40.5 78-79 34.061434948263134 35.0 34.0 37.0 28.0 39.0 80-81 33.71388146123718 35.0 34.0 37.0 27.0 39.0 82-83 33.406226893268396 35.0 34.0 36.5 27.0 39.0 84-85 33.11478868903869 35.0 34.0 36.0 26.0 37.0 86-87 32.85064887669154 35.0 34.0 36.0 26.0 37.0 88-89 32.634601848601996 35.0 34.0 35.0 26.0 36.5 90-91 32.454705252515126 35.0 33.5 35.0 25.0 36.0 92-93 32.29587691811656 35.0 33.0 35.0 25.0 36.0 94-95 32.16126332772478 35.0 33.0 35.0 25.0 36.0 96-97 32.04324183834538 35.0 33.0 35.0 25.0 36.0 98-99 31.91533058850485 35.0 33.0 35.0 24.0 35.0 100-101 30.356327777456904 33.5 30.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27161481983043245 1101 2 0.24926558989482572 1101 3 0.41030277536619053 1101 4 0.19578449837838008 1101 5 0.11127729524865515 1101 6 0.15875885107148235 1101 7 0.21649493148119348 1101 8 0.3119016813330262 1101 9 0.40270385739165704 1101 10-11 0.5618327473998548 1101 12-13 0.6690928359795905 1101 14-15 0.7412595817756085 1101 16-17 0.6298316734223093 1101 18-19 0.750599950270086 1101 20-21 0.7522257703528368 1101 22-23 0.6929530605186187 1101 24-25 0.7513019709347972 1101 26-27 0.7624062903996744 1101 28-29 0.8820401965964706 1101 30-31 0.8316009531655197 1101 32-33 0.8577299610761742 1101 34-35 0.8764877952188783 1101 36-37 0.8363211568072657 1101 38-39 0.9358943887077587 1101 40-41 0.850996906610682 1101 42-43 0.8645592732103538 1101 44-45 0.9384766272549996 1101 46-47 1.0070360569212582 1101 48-49 0.9294779319933255 1101 50-51 1.0610759161490435 1101 52-53 1.015859757838193 1101 54-55 1.0217584356568423 1101 56-57 0.9159799501256245 1101 58-59 1.0196171882067304 1101 60-61 0.937759526836615 1101 62-63 1.0490789191185144 1101 64-65 0.9235532580714718 1101 66-67 1.029353829763913 1101 68-69 0.984069581155282 1101 70-71 0.9803585992538046 1101 72-73 1.0548553680160495 1101 74-75 1.0605891182952405 1101 76-77 1.0605021939374808 1101 78-79 1.171337340244115 1101 80-81 1.1211215324888641 1101 82-83 0.9731774178376469 1101 84-85 0.9600153759277532 1101 86-87 1.0060367586395955 1101 88-89 0.9690722526717153 1101 90-91 0.926732273137084 1101 92-93 0.9231539799052086 1101 94-95 1.02644852214204 1101 96-97 1.0697706071861788 1101 98-99 1.2141870315190317 1101 100-101 1.178293801574128 1102 1 0.15486821711260745 1102 2 0.29785338632434843 1102 3 0.34910506686632914 1102 4 0.1553235338166985 1102 5 0.16799858488361963 1102 6 0.22774366322175865 1102 7 0.3061898422192755 1102 8 0.30023101786380124 1102 9 0.5060078808395616 1102 10-11 0.4287791902455851 1102 12-13 0.4820091695793778 1102 14-15 0.515590117347152 1102 16-17 0.5822264242882795 1102 18-19 0.5870141522418493 1102 20-21 0.5978259568702882 1102 22-23 0.575670220124266 1102 24-25 0.6171059943827046 1102 26-27 0.5693240894937333 1102 28-29 0.6075231435722301 1102 30-31 0.5544293571090364 1102 32-33 0.4433980932371142 1102 34-35 0.5022505183736925 1102 36-37 0.4892221693305814 1102 38-39 0.5980313458087423 1102 40-41 0.3672305863335765 1102 42-43 0.4695086470446199 1102 44-45 0.4361951191281719 1102 46-47 0.38262891596735926 1102 48-49 0.4012405219213804 1102 50-51 0.3750114344011095 1102 52-53 0.5142310607958294 1102 54-55 0.34449691154280515 1102 56-57 0.31430129305128673 1102 58-59 0.20891908002660387 1102 60-61 0.3450703448488639 1102 62-63 0.4081649836002583 1102 64-65 0.31213905609971704 1102 66-67 0.34831532723260494 1102 68-69 0.2661239376700735 1102 70-71 0.1600648342657962 1102 72-73 0.12105241038215553 1102 74-75 0.2991116336163131 1102 76-77 0.29378889790763196 1102 78-79 0.29028884570641367 1102 80-81 0.16979592630713825 1102 82-83 0.30641083110702994 1102 84-85 0.24725558376148626 1102 86-87 0.2929605529374939 1102 88-89 0.24336615088647306 1102 90-91 0.23203136719517659 1102 92-93 0.2710023678615059 1102 94-95 0.29731516216336473 1102 96-97 0.2733111081187687 1102 98-99 0.2628547639997123 1102 100-101 0.24248245065486174 1103 1 0.40270417604669007 1103 2 0.4015592541174229 1103 3 0.4576082570625388 1103 4 0.4007411228781521 1103 5 0.33416760760597697 1103 6 0.3090529450203974 1103 7 0.41346066080733124 1103 8 0.4101532323764516 1103 9 0.5268329901502327 1103 10-11 0.5349251654161336 1103 12-13 0.515905475304919 1103 14-15 0.5826653911382778 1103 16-17 0.5877544166876874 1103 18-19 0.5753538648082639 1103 20-21 0.647937571711843 1103 22-23 0.5991609271960243 1103 24-25 0.5891437376471202 1103 26-27 0.6638341232361356 1103 28-29 0.6702317873895893 1103 30-31 0.7128024410928973 1103 32-33 0.6361929508970334 1103 34-35 0.6629269083711904 1103 36-37 0.7007112811593217 1103 38-39 0.6901177926773556 1103 40-41 0.7906989182670117 1103 42-43 0.7312467547455483 1103 44-45 0.7577078871588583 1103 46-47 0.5750832193846662 1103 48-49 0.6866026600571402 1103 50-51 0.8239126470060825 1103 52-53 0.8418981511989188 1103 54-55 0.657491186173857 1103 56-57 0.6667091419715732 1103 58-59 0.6801589119464211 1103 60-61 0.6872864921630679 1103 62-63 0.7205706066000488 1103 64-65 0.6706287690429278 1103 66-67 0.6079417087959555 1103 68-69 0.6678863565964264 1103 70-71 0.6491050741551021 1103 72-73 0.5325616366487154 1103 74-75 0.601686397590484 1103 76-77 0.6795101975901545 1103 78-79 0.5935548623347344 1103 80-81 0.5143661004796201 1103 82-83 0.63798081804714 1103 84-85 0.6024162702956488 1103 86-87 0.6558722540334685 1103 88-89 0.583672875774873 1103 90-91 0.5501788796420684 1103 92-93 0.6089562252252207 1103 94-95 0.643966919800036 1103 96-97 0.6373151912916768 1103 98-99 0.608652962279379 1103 100-101 0.659383157790387 1104 1 0.161337828958537 1104 2 0.21615783624883278 1104 3 0.20445081859355696 1104 4 0.08412779918626967 1104 5 0.17392811887681603 1104 6 0.14517685547815518 1104 7 0.1297123273473204 1104 8 0.25620448271797613 1104 9 0.2830836706326565 1104 10-11 0.3113920801036656 1104 12-13 0.34722209838647444 1104 14-15 0.43934346594306106 1104 16-17 0.4121984240255969 1104 18-19 0.5029672254773061 1104 20-21 0.4210970850102669 1104 22-23 0.3639463980487818 1104 24-25 0.4557178244653812 1104 26-27 0.5024034050780912 1104 28-29 0.5008538048222633 1104 30-31 0.329127445663687 1104 32-33 0.36226385048966137 1104 34-35 0.28448931657026577 1104 36-37 0.3889245037803022 1104 38-39 0.4821382246116528 1104 40-41 0.3492237453474445 1104 42-43 0.3409389815305701 1104 44-45 0.34880214398987164 1104 46-47 0.5481466434284457 1104 48-49 0.44902467420295267 1104 50-51 0.31476388173502556 1104 52-53 0.44172928884228213 1104 54-55 0.5000175375349301 1104 56-57 0.5121713256371194 1104 58-59 0.5914643186232666 1104 60-61 0.5410107596558333 1104 62-63 0.6002461628494942 1104 64-65 0.5938567414415274 1104 66-67 0.4234690852460261 1104 68-69 0.5074642931477271 1104 70-71 0.42356366692770564 1104 72-73 0.4150025194167384 1104 74-75 0.5777935039840401 1104 76-77 0.4441765198214398 1104 78-79 0.44970267441681244 1104 80-81 0.5178899723167945 1104 82-83 0.4441799359364751 1104 84-85 0.47374294910999737 1104 86-87 0.5091476599091393 1104 88-89 0.5074837519897315 1104 90-91 0.511457118378928 1104 92-93 0.33574972012134907 1104 94-95 0.3757626025113652 1104 96-97 0.5339886850374 1104 98-99 0.4200468573505347 1104 100-101 0.5143340911554937 1105 1 0.2963433945590026 1105 2 0.22891061953986025 1105 3 0.28773212779554314 1105 4 0.20116295375683535 1105 5 0.230521539492905 1105 6 0.14921854153116954 1105 7 0.16543425542052148 1105 8 0.23742720685854835 1105 9 0.3778559325437314 1105 10-11 0.29420742977453784 1105 12-13 0.2261980180847729 1105 14-15 0.30630058431660956 1105 16-17 0.31208411817474513 1105 18-19 0.3334664706366084 1105 20-21 0.3158826965097532 1105 22-23 0.40127003133558503 1105 24-25 0.3575149146477372 1105 26-27 0.2276705931639782 1105 28-29 0.2894100664663384 1105 30-31 0.13938758595215717 1105 32-33 0.13625752710374428 1105 34-35 0.27502190625299505 1105 36-37 0.15239973538584906 1105 38-39 0.2525572053705787 1105 40-41 0.23777406838784998 1105 42-43 0.2449107785618594 1105 44-45 0.2297149809933572 1105 46-47 0.24877009865530653 1105 48-49 0.39536497288036543 1105 50-51 0.2456297007028283 1105 52-53 0.22682938130781594 1105 54-55 0.26492564132405505 1105 56-57 0.20962744377312248 1105 58-59 0.2924323110218552 1105 60-61 0.46452571610279847 1105 62-63 0.32688364692324257 1105 64-65 0.3835430555612689 1105 66-67 0.4058601437702336 1105 68-69 0.22561497270066866 1105 70-71 0.3276530090482197 1105 72-73 0.39441127007194865 1105 74-75 0.4561635388696601 1105 76-77 0.4305626389979267 1105 78-79 0.398608842515614 1105 80-81 0.3931108294781538 1105 82-83 0.40956572922596024 1105 84-85 0.484365687778066 1105 86-87 0.46789669549953317 1105 88-89 0.40720846580792625 1105 90-91 0.42214788105269463 1105 92-93 0.4467179284691625 1105 94-95 0.5395529727464989 1105 96-97 0.44167039158596566 1105 98-99 0.4413257086577147 1105 100-101 0.36210068538101225 1106 1 0.05284747044188265 1106 2 0.03321162235538111 1106 3 0.11793713153194574 1106 4 0.023724828985827173 1106 5 -0.013372590964358722 1106 6 -0.00249105514804171 1106 7 -0.13182139632534273 1106 8 0.003746530909133128 1106 9 -0.047159848312695374 1106 10-11 0.05451411884558155 1106 12-13 0.16917098637847516 1106 14-15 0.11421047831698417 1106 16-17 0.18642287806381574 1106 18-19 0.15354809591742224 1106 20-21 0.1036703023257246 1106 22-23 0.12978034188505916 1106 24-25 0.1720108888331069 1106 26-27 0.16733507975621364 1106 28-29 0.20116922603104115 1106 30-31 0.17796914782879014 1106 32-33 0.13543865390133902 1106 34-35 0.05320178976710821 1106 36-37 0.17377864299027834 1106 38-39 0.17853574714925458 1106 40-41 0.08703799496439757 1106 42-43 -0.02416393683291318 1106 44-45 0.07461618511131007 1106 46-47 0.1961825295248545 1106 48-49 0.12220244245922629 1106 50-51 0.21043195686227278 1106 52-53 0.1287555114581167 1106 54-55 0.07652654779329282 1106 56-57 0.17572936868666034 1106 58-59 0.30629910983125797 1106 60-61 0.16427345636370205 1106 62-63 0.23103430461880237 1106 64-65 0.15103687774787034 1106 66-67 0.09903116239701859 1106 68-69 0.03551004930743318 1106 70-71 0.18066901263814827 1106 72-73 0.23618461252051048 1106 74-75 0.21533503571839674 1106 76-77 0.19038127964033436 1106 78-79 0.1617485989031806 1106 80-81 0.10286122288824373 1106 82-83 0.13073800002685942 1106 84-85 0.12716313644992283 1106 86-87 0.2992364235306866 1106 88-89 0.17143989715616925 1106 90-91 0.32183474394009437 1106 92-93 0.13752427525119515 1106 94-95 0.15461986532857708 1106 96-97 0.2683692142334664 1106 98-99 0.28610665539679303 1106 100-101 0.42060005110522525 1107 1 0.06273903710143713 1107 2 0.12832243179570924 1107 3 0.1330844364506092 1107 4 0.036280105387746175 1107 5 -4.951915238251559E-4 1107 6 0.06681185820705338 1107 7 0.10636071469560449 1107 8 0.12227011005007427 1107 9 0.1339741840748303 1107 10-11 0.12370110330491002 1107 12-13 0.12791158264237623 1107 14-15 0.14732300102710383 1107 16-17 0.134199786620691 1107 18-19 0.141105872312707 1107 20-21 0.14334209965723943 1107 22-23 0.13165688557565147 1107 24-25 0.18056086613405142 1107 26-27 0.24395633651393922 1107 28-29 0.2936725202700856 1107 30-31 0.2503512565488464 1107 32-33 0.23749125907141178 1107 34-35 0.19725074361944195 1107 36-37 0.29555796570004134 1107 38-39 0.3002577691261834 1107 40-41 0.3843314534773441 1107 42-43 0.4805548559490589 1107 44-45 0.37129238862581815 1107 46-47 0.44541040768093154 1107 48-49 0.4740716410365735 1107 50-51 0.40731638991245944 1107 52-53 0.4720433168887226 1107 54-55 0.49060885553937084 1107 56-57 0.5363527475626455 1107 58-59 0.461071486541762 1107 60-61 0.49760310239119576 1107 62-63 0.4603235188952226 1107 64-65 0.5139087019819684 1107 66-67 0.5127557277300312 1107 68-69 0.349360101032957 1107 70-71 0.5136791904872382 1107 72-73 0.44753958237457425 1107 74-75 0.5346304269695707 1107 76-77 0.541373268157173 1107 78-79 0.5029826487242843 1107 80-81 0.5226056837436559 1107 82-83 0.38895213751144553 1107 84-85 0.46041402162455825 1107 86-87 0.48748304765469186 1107 88-89 0.5219325507154977 1107 90-91 0.6616061870247592 1107 92-93 0.6135367283154807 1107 94-95 0.581473156868519 1107 96-97 0.5248134449125033 1107 98-99 0.4300649015253484 1107 100-101 0.6569016361452675 1108 1 0.15629280992243366 1108 2 0.0985814662807698 1108 3 0.07193877442257701 1108 4 -0.1558904249525952 1108 5 0.09040562740246827 1108 6 0.21105892184289132 1108 7 0.16536848656494385 1108 8 0.17800217673288188 1108 9 0.19961350589365878 1108 10-11 0.16558363204246263 1108 12-13 0.17069109028485485 1108 14-15 0.1675953921082396 1108 16-17 0.16951379184646242 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-1.0644023264033535 2316 72-73 -0.8224325694134009 2316 74-75 -0.6278791395126433 2316 76-77 -0.4964630804806234 2316 78-79 -0.6100863548588222 2316 80-81 -0.6143552723275647 2316 82-83 -0.6729078909835096 2316 84-85 -0.6915388088811483 2316 86-87 -0.5908253066186901 2316 88-89 -0.6114698641911573 2316 90-91 -0.5252559814455111 2316 92-93 -0.4961748917821538 2316 94-95 -0.6070951267317923 2316 96-97 -0.529661608047924 2316 98-99 -0.5070326642289587 2316 100-101 -0.5177552227767848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 46.0 7 552.0 8 852.0 9 720.0 10 851.0 11 1261.0 12 1778.0 13 2195.0 14 2520.0 15 2859.0 16 3292.0 17 3923.0 18 4867.0 19 6106.0 20 7764.0 21 9627.0 22 11154.0 23 11509.0 24 10864.0 25 10456.0 26 10521.0 27 11299.0 28 13127.0 29 15081.0 30 18336.0 31 22736.0 32 28199.0 33 37632.0 34 54164.0 35 85426.0 36 159340.0 37 340166.0 38 510520.0 39 175604.0 40 222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21626028437981 19.49594083153451 13.671949625817719 23.615849258267964 2 14.546744699851292 21.12113147694579 41.273597030660035 23.058526792542885 3 17.352397768679126 27.4368815329573 30.903882978149483 24.306837720214094 4 11.696155484145727 18.146967857326466 40.76413029197706 29.39274636655075 5 12.519730967034768 38.618429278565394 36.125679040397465 12.73616071400237 6 28.661328066241467 39.37460054113784 19.330095984371358 12.633975408249338 7 25.6186177818934 33.61179358060485 24.00009139555297 16.76949724194878 8 24.88034481511124 35.85377727030679 21.477383726133226 17.788494188448745 9 26.12326086639176 16.2835140828488 20.77262246210734 36.8206025886521 10-11 23.408527332030523 26.698037343969066 30.30124355074262 19.592191773257788 12-13 24.888564067965287 24.53646904705538 29.576552978638194 20.99841390634114 14-15 22.14488860849636 25.772435228162017 27.186876614099415 24.89579954924221 16-17 21.537520730605895 28.87924933785826 27.534655733896766 22.048574197639077 18-19 21.485063491348207 28.100990816651 29.381639268099335 21.03230642390146 20-21 22.47127863013299 27.389882639224304 29.251717950784762 20.88712077985794 22-23 22.10725141202956 27.19925309523099 28.984671569445702 21.708823923293743 24-25 21.85055049953382 27.427646773959125 29.023102130087608 21.69870059641945 26-27 21.85150253654394 27.65762718103745 28.88559625125907 21.605274031159535 28-29 21.843600629359933 27.577306991950213 28.966424193418376 21.61266818527148 30-31 22.09998419618563 27.554013819769242 28.871125288705223 21.474876695339905 32-33 21.821989389230176 27.611643793448586 28.93478483011534 21.63158198720589 34-35 22.008271297543935 27.612627565025715 28.812955827386805 21.566145310043545 36-37 22.191411483724295 27.48603837724657 28.702487799645716 21.620062339383423 38-39 21.944548286999808 27.812142787780157 28.63375072751495 21.609558197705084 40-41 22.059649561523486 27.578957189434423 28.79267743907122 21.568715809970872 42-43 21.935345262568635 27.502540352088673 28.73723715051515 21.824877234827543 44-45 21.971268792417213 27.801797318936845 28.48710529338925 21.739828595256697 46-47 22.05523471867588 27.657186270085628 28.345509902149512 21.942069109088983 48-49 21.97552749364672 27.726547445867006 28.41255575781117 21.885369302675105 50-51 21.96707982957268 27.98436628291113 28.090772286075698 21.957781601440495 52-53 22.06466362311013 27.977638554706267 28.076333058088853 21.881364764094748 54-55 21.988183316630373 27.881958835189064 28.154368358351807 21.975489489828753 56-57 21.966889422170656 28.01365728825586 28.101498569723066 21.917954719850417 58-59 22.018870642923286 27.84168766966093 28.128758562779545 22.010683124636245 60-61 22.048796339608103 27.830929651446557 28.223867060090672 21.89640694885467 62-63 21.86632257933483 27.8335953550749 28.247953596446745 22.05212846914353 64-65 21.922334090096975 27.6924717356079 28.18473833897468 22.200455835320447 66-67 21.98259803283766 27.798401720267407 27.971894598078535 22.247105648816394 68-69 21.915162077954058 27.726998944508303 28.070747774296144 22.287091203241495 70-71 21.932362213270252 27.544937733606083 28.06325841648319 22.459441636640477 72-73 21.906466806594953 27.600568429564177 27.95716975898866 22.535795004852215 74-75 21.867211147210945 27.651153329368626 27.95726496268967 22.52437056073076 76-77 21.884443017094142 27.627828422620652 27.96408789459554 22.523640665689665 78-79 21.867211147210945 27.647059570225107 27.817505929603847 22.668223352960105 80-81 21.79660173562694 27.670606618942113 27.791737461196558 22.741054184234393 82-83 21.860705560975113 27.64058571855628 27.894113174351613 22.60459554611699 84-85 21.91037015833645 27.495050994275722 27.893986236083602 22.70059261130423 86-87 21.83826922210325 27.50219127185163 27.97167245610951 22.687867049935612 88-89 21.97679060707592 27.3459302639237 27.874659884778136 22.802619244222246 90-91 21.917129621108312 27.344153128171474 27.947141635815377 22.79157561490484 92-93 21.951022138668634 27.298042802314594 27.954884870164364 22.79605018885241 94-95 21.882761085042926 27.22016617488666 27.98627035693137 22.910802383139046 96-97 21.883141899846976 27.02547460631683 28.04526491699189 23.0461185768443 98-99 21.837444123361145 27.086531913232616 27.874437742809103 23.201586220597132 100-101 22.162882107987656 26.866579629327564 27.899603254443317 23.070935008241467 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 45.0 1 35.0 2 52.5 3 89.0 4 85.0 5 93.0 6 155.5 7 316.0 8 599.5 9 799.0 10 782.5 11 733.0 12 794.5 13 912.0 14 1008.5 15 1088.5 16 1190.0 17 1323.5 18 1480.0 19 1695.0 20 1919.5 21 2347.5 22 3034.0 23 3868.0 24 4991.0 25 6531.0 26 8921.5 27 11991.5 28 14817.0 29 17696.5 30 21238.0 31 25362.0 32 29304.5 33 33114.5 34 37383.0 35 40657.0 36 43977.0 37 47595.0 38 50142.0 39 52124.0 40 53613.0 41 55087.0 42 56426.5 43 58246.5 44 59796.5 45 61035.0 46 61987.5 47 61555.0 48 61103.0 49 60220.0 50 58009.5 51 55773.5 52 53875.5 53 50985.5 54 46888.5 55 43032.5 56 38699.5 57 33366.0 58 28855.0 59 24696.0 60 20163.0 61 16120.0 62 12545.5 63 9093.5 64 6470.5 65 4652.5 66 3282.0 67 2464.0 68 1928.5 69 1488.0 70 1066.0 71 757.5 72 577.5 73 390.5 74 247.0 75 180.0 76 145.0 77 107.0 78 70.5 79 51.5 80 43.0 81 37.5 82 30.0 83 21.5 84 23.0 85 24.0 86 16.5 87 7.5 88 8.0 89 8.0 90 6.5 91 4.5 92 2.5 93 4.5 94 4.5 95 2.5 96 1.0 97 1.0 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.2693826801618972E-4 48-49 3.1734567004047424E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1575569.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.466558506923135 #Duplication Level Percentage of deduplicated Percentage of total 1 70.72588848021307 40.643734083022856 2 15.632545731909945 17.96697207829909 3 5.951101352524033 10.259679421663554 4 2.9084995721037834 6.68565843310652 5 1.5618824635578388 4.48780049864919 6 0.8972715997456663 3.0937866530030913 7 0.6135976137884974 2.468294022073958 8 0.40448924602494507 1.8595683937690985 9 0.2807075206985528 1.451816564540105 >10 0.9893959298235747 8.856995442096109 >50 0.026617292691114514 1.0243932804312386 >100 0.00766977943951332 0.6749661750176693 >500 0.0 0.0 >1k 3.3341747945255884E-4 0.5263349543275554 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3870 0.24562554861132707 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2731 0.17333420497610705 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1640 0.10408937977327555 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.346913400809485E-5 0.0 8 0.0 0.0 0.0 6.346913400809485E-5 0.0 9 0.0 0.0 0.0 6.346913400809485E-5 0.0 10-11 0.0 0.0 0.0 9.520370101214227E-5 0.0 12-13 0.0 0.0 0.0 2.22141969028332E-4 0.0 14-15 0.0 0.0 0.0 2.856111030364268E-4 0.0 16-17 0.0 0.0 0.0 6.664259070849959E-4 0.0 18-19 0.0 0.0 0.0 7.616296080971383E-4 0.0 20-21 0.0 0.0 0.0 8.250987421052332E-4 0.0 22-23 0.0 0.0 0.0 0.0012693826801618972 0.0 24-25 0.0 0.0 0.0 0.001999277721254988 0.0 26-27 0.0 0.0 0.0 0.0024752962263156993 0.0 28-29 0.0 0.0 0.0 0.006251709699797343 0.0 30-31 0.0 0.0 0.0 0.017104931615181562 0.0 32-33 0.0 0.0 0.0 0.0400172889921038 0.0 34-35 0.0 0.0 0.0 0.07441755962449122 0.0 36-37 0.0 0.0 0.0 0.13331691598400325 0.0 38-39 0.0 0.0 0.0 0.22823500589310908 0.0 40-41 0.0 0.0 0.0 0.3329590770064656 0.0 42-43 0.0 0.0 0.0 0.43565213583156304 0.0 44-45 0.0 0.0 0.0 0.5547837003647571 0.0 46-47 0.0 0.0 0.0 0.6983508814910677 0.0 48-49 0.0 0.0 0.0 0.8584200374594829 0.0 50-51 0.0 0.0 0.0 1.0215674464272908 0.0 52-53 0.0 0.0 0.0 1.176209991438014 0.0 54-55 0.0 0.0 0.0 1.3324709993659434 0.0 56-57 0.0 0.0 0.0 1.5201809631948837 0.0 58-59 0.0 0.0 0.0 1.7322313399159288 0.0 60-61 0.0 0.0 0.0 1.9550397348513457 0.0 62-63 0.0 0.0 0.0 2.178514555693848 0.0 64-65 0.0 0.0 0.0 2.394753895259427 0.0 66-67 0.0 0.0 0.0 2.635048036614074 0.0 68-69 0.0 0.0 0.0 2.913582331208598 0.0 70-71 0.0 0.0 0.0 3.2129662363247817 0.0 72-73 0.0 0.0 0.0 3.5073360798543254 0.0 74-75 0.0 0.0 0.0 3.800023991332655 0.0 76-77 0.0 0.0 0.0 4.101121563067057 0.0 78-79 0.0 0.0 0.0 4.434175843774534 0.0 80-81 0.0 0.0 0.0 4.801186111176343 3.1734567004047424E-5 82-83 0.0 0.0 0.0 5.18206438435892 6.346913400809485E-5 84-85 0.0 0.0 0.0 5.564148571087651 6.346913400809485E-5 86-87 0.0 0.0 0.0 5.974286115047961 6.346913400809485E-5 88-89 0.0 0.0 0.0 6.408478460797337 6.346913400809485E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1950 0.0 50.179493 1 GTATCAA 3880 0.0 47.622417 1 TCAACGC 4630 0.0 39.190063 4 CAACGCA 4700 0.0 38.70745 5 ATCAACG 4700 0.0 38.70745 3 AACGCAG 4865 0.0 37.492294 6 TATCAAC 5005 0.0 36.91808 2 GCGTTAG 55 0.002579915 34.545452 1 ACGCAGA 5570 0.0 32.66158 7 CGCAGAG 5635 0.0 32.03194 8 GCAGAGT 6360 0.0 28.081762 9 GTACATG 4580 0.0 26.550217 1 TACATGG 4510 0.0 26.4357 2 ACATGGG 4500 0.0 24.91111 3 AGAGTAC 5730 0.0 23.00393 10-11 CATGGGG 2785 0.0 22.172352 4 CAGAGTA 6055 0.0 21.88687 10-11 GAGTACT 4120 0.0 21.213593 12-13 GTACTTT 4260 0.0 20.349178 14-15 AGTACTT 4395 0.0 19.832195 12-13 >>END_MODULE