FastQCFastQC Report
Fri 10 Feb 2017
SRR2031530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575569
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47670.30255736181658816No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.21687403090566013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21240.13480844063319347No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT19150.12154339162550165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21050.054.2344931
GTATCAA41350.047.3956151
TCAACGC49850.038.6850244
ATCAACG50300.038.3389363
CAACGCA50200.038.320695
AACGCAG52200.037.3984226
TATCAAC52250.036.9990122
ACGCAGA57500.033.620847
CGCAGAG58600.032.7465638
GTATACG650.00580390429.2727181
GCAGAGT67850.027.9321949
CGTCGCG350.00839224727.14385442-43
GTACATG42400.025.579111
TACATGG40750.025.0606822
ACATGGG40850.024.8830553
GTACCGG1401.7603757E-523.7840841
ACCGTGC2002.0558218E-823.7493678
AGAGTAC62300.022.87258110-11
TACCGGA1252.2568277E-422.7993892
GAGTACT45500.022.02688412-13
Produced by FastQC (version 0.11.5)