FastQCFastQC Report
Fri 10 Feb 2017
SRR2031529_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031529_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007810
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36970.18413096856774297No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25660.1278009373396885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22150.057.9006771
GTATCAA43500.044.005751
TCAACGC51050.036.9392744
ATCAACG51950.036.4821933
CAACGCA52600.035.9410635
AACGCAG54550.034.482136
TATCAAC55750.034.165922
ACGCAGA63100.029.7345477
CGCAGAG64450.028.9643158
GTACATG59050.027.1888241
TACATGG59600.026.539432
ACATGGG59550.025.8438283
GCAGAGT72700.024.632059
CATGGGG39950.023.6608264
AGAGTAC65900.020.86684210-11
CAGAGTA69750.020.73655910-11
CCGTGCG1404.8534747E-420.3571439
GAGTACT41850.019.57885412-13
TATACTG13350.019.569295
ATGGGGG21700.017.2926275
Produced by FastQC (version 0.11.5)