##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031529_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2007810 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.17132447791375 34.0 31.0 34.0 30.0 34.0 2 32.33139789123473 34.0 31.0 34.0 30.0 34.0 3 32.436791827912 34.0 31.0 34.0 30.0 34.0 4 35.669412942459694 37.0 35.0 37.0 35.0 37.0 5 35.65214885870675 37.0 35.0 37.0 35.0 37.0 6 35.77421020913334 37.0 37.0 37.0 35.0 37.0 7 35.80221584711701 37.0 37.0 37.0 35.0 37.0 8 35.84754035491406 37.0 37.0 37.0 35.0 37.0 9 37.66349256154716 39.0 39.0 39.0 35.0 39.0 10-11 37.66208655201439 39.0 38.5 39.0 35.0 39.0 12-13 37.63362793292194 39.0 39.0 39.0 35.0 39.0 14-15 39.14230081531619 41.0 39.0 41.0 36.0 41.0 16-17 39.154440161170626 41.0 39.0 41.0 36.0 41.0 18-19 39.107079106090715 41.0 39.5 41.0 36.0 41.0 20-21 39.06814688640857 41.0 39.0 41.0 36.0 41.0 22-23 38.9975851798726 41.0 39.0 41.0 36.0 41.0 24-25 38.94947056743417 41.0 39.0 41.0 35.5 41.0 26-27 38.84236705664381 41.0 39.0 41.0 35.0 41.0 28-29 38.74302971894751 41.0 39.0 41.0 35.0 41.0 30-31 38.58466239335395 40.0 38.5 41.0 34.0 41.0 32-33 38.48312016575274 40.0 38.0 41.0 34.0 41.0 34-35 38.35696505147399 40.0 38.0 41.0 34.0 41.0 36-37 38.22251707083838 40.0 38.0 41.0 34.0 41.0 38-39 38.07934266688581 40.0 38.0 41.0 33.0 41.0 40-41 37.955544847371016 40.0 38.0 41.0 33.0 41.0 42-43 37.81659345256772 40.0 38.0 41.0 33.0 41.0 44-45 37.997677569092694 40.0 38.0 41.0 33.0 41.0 46-47 37.88630796738735 40.0 38.0 41.0 33.0 41.0 48-49 37.770482515775896 40.0 38.0 41.0 33.0 41.0 50-51 36.72347458175824 39.0 36.5 40.0 31.0 40.5 52-53 36.7953342198714 39.5 37.0 40.0 31.5 40.5 54-55 37.58967457080102 40.0 38.0 41.0 32.0 41.0 56-57 37.63466363849169 40.0 37.0 41.0 32.0 41.0 58-59 37.44656292179041 40.0 37.0 41.0 32.0 41.0 60-61 37.282042872582565 40.0 36.5 41.0 32.0 41.0 62-63 37.02949108730408 39.5 36.0 41.0 31.0 41.0 64-65 36.7606232661457 39.0 35.5 41.0 31.0 41.0 66-67 36.47579327725233 39.0 35.0 41.0 31.0 41.0 68-69 36.1736277336999 38.5 35.0 41.0 30.0 41.0 70-71 35.811885088728516 37.0 35.0 40.0 30.0 41.0 72-73 35.45781074902506 37.0 35.0 39.5 30.0 41.0 74-75 35.07366882324523 36.0 35.0 39.0 29.0 41.0 76-77 34.697843670466824 36.0 35.0 39.0 29.0 40.5 78-79 34.29070803512285 35.0 34.0 37.5 29.0 39.5 80-81 33.93212081820491 35.0 34.0 37.0 28.5 39.0 82-83 33.61476011176356 35.0 34.0 36.5 28.0 39.0 84-85 33.306362902864315 35.0 34.0 36.0 27.0 37.5 86-87 33.03473486037025 35.0 34.0 36.0 27.0 37.0 88-89 32.79987822552931 35.0 34.0 35.5 26.0 37.0 90-91 32.59819305611587 35.0 34.0 35.0 26.0 36.0 92-93 32.433363714694124 35.0 34.0 35.0 25.5 36.0 94-95 32.29193026232562 35.0 33.0 35.0 25.0 36.0 96-97 32.158257006390045 35.0 33.0 35.0 25.0 36.0 98-99 32.01426454694418 35.0 33.0 35.0 24.5 35.0 100-101 30.442261468963697 34.0 30.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15088009172460204 1101 2 0.17512276830715479 1101 3 0.2923388224414154 1101 4 0.022189707854415985 1101 5 0.08472880376438496 1101 6 0.02052764398998619 1101 7 0.07924758938502663 1101 8 0.22543209171075773 1101 9 0.3971172174997264 1101 10-11 0.5458779525814137 1101 12-13 0.629787330164973 1101 14-15 0.7705900328397277 1101 16-17 0.7214047486222128 1101 18-19 0.8335089178190742 1101 20-21 0.7157087166620997 1101 22-23 0.7798894197841335 1101 24-25 0.742762206801018 1101 26-27 0.7339333474134335 1101 28-29 0.7995712719173724 1101 30-31 0.7616637030320064 1101 32-33 0.7781325866027444 1101 34-35 0.8526510481154688 1101 36-37 0.8061060103631874 1101 38-39 0.8108430939306999 1101 40-41 0.9386275929551786 1101 42-43 0.8445644797773113 1101 44-45 1.000747134859715 1101 46-47 1.043650553617809 1101 48-49 1.0090827911752172 1101 50-51 0.9718863971949858 1101 52-53 1.0794485835991736 1101 54-55 1.0507663414892932 1101 56-57 0.9358564432265695 1101 58-59 0.9323785554062383 1101 60-61 0.8668711799316426 1101 62-63 0.9372920962091555 1101 64-65 0.941344997736536 1101 66-67 1.0383979805461578 1101 68-69 0.9497482838813269 1101 70-71 1.0157999248579301 1101 72-73 1.000981099245287 1101 74-75 0.9692766794909744 1101 76-77 1.0547230468631525 1101 78-79 1.026417716147236 1101 80-81 0.9670395803627656 1101 82-83 0.9272520820264418 1101 84-85 0.92705325016302 1101 86-87 0.9443362891694846 1101 88-89 0.912211833673858 1101 90-91 0.8914466377683183 1101 92-93 0.95334803483982 1101 94-95 0.9054223775366737 1101 96-97 0.8819506737921756 1101 98-99 0.9193018883219466 1101 100-101 0.9800508580121345 1102 1 0.2122460245771407 1102 2 0.2624493365278724 1102 3 0.38795618427246126 1102 4 0.18157447379063996 1102 5 0.15254458973537055 1102 6 0.1645859241916341 1102 7 0.256456858576648 1102 8 0.32652821992419945 1102 9 0.4594279915008457 1102 10-11 0.37571421760935664 1102 12-13 0.3903099360518567 1102 14-15 0.5154365242733476 1102 16-17 0.5212854249335024 1102 18-19 0.5706532666754214 1102 20-21 0.6467414055412846 1102 22-23 0.6205168392992277 1102 24-25 0.5345076883419324 1102 26-27 0.614230972375239 1102 28-29 0.6614604293027853 1102 30-31 0.675971391301907 1102 32-33 0.6259025552767596 1102 34-35 0.6485508312144361 1102 36-37 0.6214036259515723 1102 38-39 0.577782277280491 1102 40-41 0.545745208806018 1102 42-43 0.5479841860891881 1102 44-45 0.6229409660218508 1102 46-47 0.692084816857303 1102 48-49 0.754681499892321 1102 50-51 0.8168565240924011 1102 52-53 0.8366623707811982 1102 54-55 0.7723039392306674 1102 56-57 0.686380417633373 1102 58-59 0.7247492443341557 1102 60-61 0.730429744148033 1102 62-63 0.7030924085316528 1102 64-65 0.7689405457350276 1102 66-67 0.7684621135138059 1102 68-69 0.7279482302653335 1102 70-71 0.718251356460982 1102 72-73 0.6855241354473307 1102 74-75 0.7599109416056535 1102 76-77 0.7250672202490094 1102 78-79 0.6675083702809985 1102 80-81 0.5273261634309847 1102 82-83 0.6091518126342308 1102 84-85 0.607524002267823 1102 86-87 0.5814568295286406 1102 88-89 0.6372325958082214 1102 90-91 0.5634067894678125 1102 92-93 0.573783411642097 1102 94-95 0.5791419209384401 1102 96-97 0.500560576252326 1102 98-99 0.5552577807375343 1102 100-101 0.3727359315841383 1103 1 0.2375524190060858 1103 2 0.23758742505692965 1103 3 0.3424284298601208 1103 4 0.27793987314550606 1103 5 0.24451446350220607 1103 6 0.20531625077828863 1103 7 0.281205968490184 1103 8 0.33939581480076697 1103 9 0.39230284751077704 1103 10-11 0.4358568704928203 1103 12-13 0.4364629547261174 1103 14-15 0.4755428714899992 1103 16-17 0.3400910133168864 1103 18-19 0.31647624741549407 1103 20-21 0.4419994820580939 1103 22-23 0.479347931293006 1103 24-25 0.41243287177519505 1103 26-27 0.4533647460579573 1103 28-29 0.47662536609656314 1103 30-31 0.5328628194293898 1103 32-33 0.482027903953103 1103 34-35 0.5106774468539612 1103 36-37 0.5531418755403337 1103 38-39 0.5943062113804842 1103 40-41 0.5474331235011434 1103 42-43 0.4895948130417409 1103 44-45 0.5768301545528516 1103 46-47 0.6275791341987116 1103 48-49 0.6402521637182801 1103 50-51 0.5911368725670414 1103 52-53 0.6312466260742866 1103 54-55 0.653124238132385 1103 56-57 0.6454771535597033 1103 58-59 0.6043765360359998 1103 60-61 0.5807928755192648 1103 62-63 0.6966944685420771 1103 64-65 0.5469015119139868 1103 66-67 0.4787313133100142 1103 68-69 0.5189527169286023 1103 70-71 0.5058986894885322 1103 72-73 0.5319182954641803 1103 74-75 0.5641490679344301 1103 76-77 0.452625686566158 1103 78-79 0.4874692408400989 1103 80-81 0.41835985897435535 1103 82-83 0.4361295376882808 1103 84-85 0.4778104285800211 1103 86-87 0.5134770830109261 1103 88-89 0.545739700812284 1103 90-91 0.38637856517350144 1103 92-93 0.4397796436218613 1103 94-95 0.46351472186834286 1103 96-97 0.47242854016180047 1103 98-99 0.4035027080290874 1103 100-101 0.3349974608744475 1104 1 0.15260293878968412 1104 2 0.1647540740438771 1104 3 0.29586271436208733 1104 4 0.15542210503264187 1104 5 0.0764082260990051 1104 6 0.17360481023516172 1104 7 0.18765884321172877 1104 8 0.17010216358617214 1104 9 0.4132692206941897 1104 10-11 0.3381421945619749 1104 12-13 0.4563350640928334 1104 14-15 0.38618716447822266 1104 16-17 0.3884269102087927 1104 18-19 0.44334852915942236 1104 20-21 0.4394870841584506 1104 22-23 0.4008705524874472 1104 24-25 0.4881568560756051 1104 26-27 0.4588052361396393 1104 28-29 0.44010559575604447 1104 30-31 0.44801444507911725 1104 32-33 0.42374186385551127 1104 34-35 0.42699593364721267 1104 36-37 0.4102600600104651 1104 38-39 0.4466323092907416 1104 40-41 0.5299401511875672 1104 42-43 0.5272615548471933 1104 44-45 0.49767288023439704 1104 46-47 0.5909244455384766 1104 48-49 0.5389364614800272 1104 50-51 0.4313491617364491 1104 52-53 0.5193128189246039 1104 54-55 0.44689180734846445 1104 56-57 0.3931745917690961 1104 58-59 0.449969314756693 1104 60-61 0.3730113702683937 1104 62-63 0.3880195852281929 1104 64-65 0.4631546942625562 1104 66-67 0.4946825040387708 1104 68-69 0.5001513352589342 1104 70-71 0.403106510110149 1104 72-73 0.3873127077904783 1104 74-75 0.4611553398130823 1104 76-77 0.4280201651913913 1104 78-79 0.44402885683670235 1104 80-81 0.3800470341096087 1104 82-83 0.4170543414434533 1104 84-85 0.594219744905466 1104 86-87 0.49137600366435663 1104 88-89 0.5593276662307574 1104 90-91 0.5437805090580881 1104 92-93 0.47144666388280854 1104 94-95 0.36660590941475135 1104 96-97 0.3923659539731901 1104 98-99 0.3702905912368948 1104 100-101 0.5174302337608339 1105 1 0.2515199402964683 1105 2 0.2968484989559812 1105 3 0.3097627645881005 1105 4 0.260181020150668 1105 5 0.2067384621612831 1105 6 0.15580910455962282 1105 7 0.16701281106707455 1105 8 0.17598174671022804 1105 9 0.38642341456661455 1105 10-11 0.41786657929326765 1105 12-13 0.4052065482716287 1105 14-15 0.4292716697519481 1105 16-17 0.41298115430723215 1105 18-19 0.4709602820474146 1105 20-21 0.4408165040479517 1105 22-23 0.4246933802122044 1105 24-25 0.4559870921378888 1105 26-27 0.409288661554001 1105 28-29 0.3903488780262663 1105 30-31 0.4268457484442578 1105 32-33 0.31832373226334454 1105 34-35 0.4370427066256184 1105 36-37 0.40160078075381733 1105 38-39 0.3615415885473965 1105 40-41 0.24750983978626806 1105 42-43 0.2634258567055525 1105 44-45 0.26620785009878745 1105 46-47 0.29532863941090426 1105 48-49 0.4236379305910276 1105 50-51 0.3508086042276588 1105 52-53 0.29811743971817606 1105 54-55 0.3593933769114983 1105 56-57 0.4209150488258544 1105 58-59 0.48917542977427075 1105 60-61 0.411927116124744 1105 62-63 0.47198022058730515 1105 64-65 0.4133336244483985 1105 66-67 0.3632990911809131 1105 68-69 0.35805094600730314 1105 70-71 0.3241935894672565 1105 72-73 0.3185077730842991 1105 74-75 0.2503991243434669 1105 76-77 0.29405796026923525 1105 78-79 0.33288495929777184 1105 80-81 0.5349064012617859 1105 82-83 0.48635839719432994 1105 84-85 0.5647731728311314 1105 86-87 0.4128021086367397 1105 88-89 0.43038806301629506 1105 90-91 0.4465350921821454 1105 92-93 0.4714089943700017 1105 94-95 0.5182743549195195 1105 96-97 0.5245859439279883 1105 98-99 0.48509627933332666 1105 100-101 0.4848994703253737 1106 1 0.11832163314456778 1106 2 0.13677871430568445 1106 3 0.2203573964930854 1106 4 0.16264454114602245 1106 5 0.044262189234864024 1106 6 0.06434997362127604 1106 7 0.08921901213227557 1106 8 0.10070908644097898 1106 9 0.1416945539633545 1106 10-11 0.07214798165471592 1106 12-13 0.2848369470034626 1106 14-15 0.2235858001199702 1106 16-17 0.26955629564601225 1106 18-19 0.2837198174793585 1106 20-21 0.2942928676417722 1106 22-23 0.1778307647209516 1106 24-25 0.27874109978319694 1106 26-27 0.2882057125822044 1106 28-29 0.2907599523179627 1106 30-31 0.34720789158761534 1106 32-33 0.26655732658230846 1106 34-35 0.2649135728502543 1106 36-37 0.304406644302297 1106 38-39 0.3961099971005524 1106 40-41 0.32032497480757627 1106 42-43 0.4207449094359603 1106 44-45 0.44928463368740523 1106 46-47 0.33230967822532875 1106 48-49 0.4550237671732731 1106 50-51 0.2899524992406626 1106 52-53 0.36887968932260407 1106 54-55 0.401063236435526 1106 56-57 0.4394596030785962 1106 58-59 0.48531855557675385 1106 60-61 0.4628307191302241 1106 62-63 0.506711684222374 1106 64-65 0.46417405274949175 1106 66-67 0.5038650019934394 1106 68-69 0.41043169644090227 1106 70-71 0.5068769935309163 1106 72-73 0.4835142514162527 1106 74-75 0.5201233784578676 1106 76-77 0.5403935349396889 1106 78-79 0.5016573519519625 1106 80-81 0.4993970990790473 1106 82-83 0.4865281792123497 1106 84-85 0.48261593216182774 1106 86-87 0.43796977020750205 1106 88-89 0.4781354168443599 1106 90-91 0.5465138877096081 1106 92-93 0.5577272837393963 1106 94-95 0.486071926788064 1106 96-97 0.5500371604797678 1106 98-99 0.4948112912224545 1106 100-101 0.5509018276777731 1107 1 0.1991200163448994 1107 2 0.17815931287285025 1107 3 0.19646620691472805 1107 4 0.10944110862791234 1107 5 0.13713626924230482 1107 6 0.16933863149864692 1107 7 0.167945823180915 1107 8 0.22515159861442413 1107 9 0.3235671148903876 1107 10-11 0.34194612368370514 1107 12-13 0.23177965834039327 1107 14-15 0.3379623314016911 1107 16-17 0.2567307109278474 1107 18-19 0.3598739528882362 1107 20-21 0.45184423618720615 1107 22-23 0.362378641855706 1107 24-25 0.33928679957843144 1107 26-27 0.34335197471180834 1107 28-29 0.34400743191424255 1107 30-31 0.3899716227884156 1107 32-33 0.3138099172710227 1107 34-35 0.3991277389887671 1107 36-37 0.4332810538471392 1107 38-39 0.4459886909034765 1107 40-41 0.417411260586654 1107 42-43 0.419680982171279 1107 44-45 0.2930455689863294 1107 46-47 0.4776957603818275 1107 48-49 0.3818887598878362 1107 50-51 0.29026993728857775 1107 52-53 0.319749388005242 1107 54-55 0.2916674236812895 1107 56-57 0.3173945455441398 1107 58-59 0.3490303702266502 1107 60-61 0.41192058958015565 1107 62-63 0.4836811799893681 1107 64-65 0.4420870260550629 1107 66-67 0.3587056807208526 1107 68-69 0.2604829351562117 1107 70-71 0.3524628917228938 1107 72-73 0.2554800806718376 1107 74-75 0.3874762822886808 1107 76-77 0.35365254834496085 1107 78-79 0.29341894415051684 1107 80-81 0.39862690057082517 1107 82-83 0.28734035839260486 1107 84-85 0.4030201429537428 1107 86-87 0.37721158404443145 1107 88-89 0.43318809252762236 1107 90-91 0.42695815415310534 1107 92-93 0.4502473829377678 1107 94-95 0.4310303444429948 1107 96-97 0.3696386812459238 1107 98-99 0.48840339298316593 1107 100-101 0.4998165973971993 1108 1 0.1862119865385381 1108 2 0.17680713842078433 1108 3 0.07427691635199807 1108 4 0.011343008217409078 1108 5 0.23841074756554548 1108 6 0.16892050466067587 1108 7 0.15128697232898958 1108 8 0.17291808517083496 1108 9 0.24846753063012983 1108 10-11 0.16022689085020403 1108 12-13 0.18679213669289396 1108 14-15 0.2733574997651189 1108 16-17 0.12637100634254494 1108 18-19 0.14323381378983413 1108 20-21 0.32363407316394444 1108 22-23 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-0.6603937975438186 2316 86-87 -0.5067856229321848 2316 88-89 -0.6612536635964332 2316 90-91 -0.6219431010095349 2316 92-93 -0.6341694142539325 2316 94-95 -0.6005545902095903 2316 96-97 -0.6472659017743645 2316 98-99 -0.5213392923093139 2316 100-101 -0.531175138139993 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 39.0 7 715.0 8 1150.0 9 997.0 10 1184.0 11 1703.0 12 2265.0 13 2807.0 14 3061.0 15 3343.0 16 4052.0 17 4826.0 18 5793.0 19 7069.0 20 8949.0 21 11055.0 22 12512.0 23 12759.0 24 12120.0 25 11791.0 26 12273.0 27 13644.0 28 16047.0 29 18462.0 30 22457.0 31 27880.0 32 34859.0 33 46727.0 34 67961.0 35 106451.0 36 200584.0 37 429300.0 38 658671.0 39 244083.0 40 221.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.546207061425136 20.11629586464855 13.726647441740006 23.610849632186312 2 14.296472275763145 21.424088932717737 41.601894601580824 22.677544189938292 3 17.070439932065284 27.59085770067885 31.202653637545385 24.13604872971048 4 11.564042414371878 18.276131705689284 41.10139903676145 29.058426843177394 5 12.357743013532158 38.80486699438692 36.255422574845234 12.581967417235695 6 28.124971984400915 39.865525124389265 19.547865584891 12.461637306318824 7 25.344430000846696 33.71608867372909 24.40335489911894 16.536126426305277 8 24.378003894790844 36.280375135097444 21.919554141079086 17.42206682903263 9 25.94533347278876 16.28416035381834 21.29210433258127 36.47840184081163 10-11 23.051259830362433 26.810056728475306 30.775845324009744 19.36283811715252 12-13 24.472211015982587 24.731299276325945 29.953506556895327 20.84298315079614 14-15 21.780621672369396 26.119926686290036 27.436012371688555 24.66343926965201 16-17 21.26306772055125 29.150915674291888 27.87733899123921 21.70867761391765 18-19 21.385489662866505 28.371733381146626 29.497362798272746 20.745414157714126 20-21 22.24587983922782 27.655330932707777 29.20293752894945 20.895851699114957 22-23 21.94986079360099 27.54593313112296 28.86816979694294 21.63603627833311 24-25 21.675880685921474 27.759797988853528 28.93525781821985 21.629063507005146 26-27 21.580403524237852 27.933843341750464 28.936901400032873 21.548851733978815 28-29 21.71923638192857 27.842251009806706 28.947734098345958 21.49077850991877 30-31 22.02287567050667 27.80126107550017 28.789701216748597 21.38616203724456 32-33 21.758582734422085 27.93399275827892 28.758124523734818 21.549299983564183 34-35 21.866336954193873 28.015499474551874 28.726273900418864 21.391889670835386 36-37 22.023348822846785 27.783654827897063 28.649772637849196 21.543223711406956 38-39 21.80300924888311 28.079648970769146 28.66195008491839 21.45539169542935 40-41 21.88765371225365 27.809678206603216 28.81119229409157 21.491475787051563 42-43 21.844273113491813 27.79177312594319 28.691410043779044 21.67254371678595 44-45 21.908646734501772 27.950079937842727 28.50090396999716 21.640369357658344 46-47 21.98003992413617 27.810104352607425 28.385757004653833 21.824098718602574 48-49 21.805966313478496 27.90047942878944 28.42560486421497 21.8679493935171 50-51 21.745882329503292 28.09720541286277 28.1724615376953 21.98445071993864 52-53 21.83508399699175 28.105647446720557 28.174702785622145 21.88456577066555 54-55 21.773648901041433 28.000557821706234 28.295306826841188 21.930486450411145 56-57 21.83956649284544 28.05808318516194 28.179235086985322 21.923115235007295 58-59 21.738087767268816 27.928812985292435 28.22632619620382 22.106773051234928 60-61 21.91609265816985 27.965594354047447 28.21643980257096 21.90187318521175 62-63 21.68868070185924 27.91123164044407 28.393573097055995 22.006514560640696 64-65 21.72755390201264 27.766895273955207 28.35228432969255 22.153266494339604 66-67 21.753676891737765 27.921342158869617 28.040327521030378 22.284653428362244 68-69 21.79167849547517 27.816451755893233 28.094217082293643 22.29765266633795 70-71 21.78455630761875 27.635284215139876 28.261040636315187 22.319118840926183 72-73 21.81302015628969 27.715321668883014 28.106792973438722 22.36486520138858 74-75 21.758234095855684 27.936084589677314 27.91501685916496 22.390664455302044 76-77 21.793346980042934 27.857342079180796 27.971546112430957 22.377764828345313 78-79 21.848008526703225 27.78243459291467 27.8874744124195 22.482082467962606 80-81 21.854757173238504 27.877936657353036 27.814708563061245 22.45259760634721 82-83 21.88483970096772 27.828031536848606 27.937105602621763 22.35002315956191 84-85 22.041403320035265 27.74752093076536 27.78343070310438 22.427645046094998 86-87 21.87380778061669 27.819066545141226 27.869793456552166 22.43733221768992 88-89 22.072183124897276 27.625796265582895 27.827732703791693 22.474287905728133 90-91 22.1570517130605 27.581070918064952 27.879107086825943 22.3827702820486 92-93 22.19886343827354 27.58866625826149 27.866232362623954 22.346237940841014 94-95 22.16536923314457 27.473889461652245 27.854453359630643 22.506287945572538 96-97 22.212186412060902 27.420846594050236 27.824470442920397 22.54249655096847 98-99 22.27805419835542 27.224861914224952 27.799991035008293 22.697092852411334 100-101 22.612622708323997 27.26398414192578 27.684990113606368 22.438403036143857 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 34.0 1 31.0 2 44.5 3 81.5 4 94.0 5 101.5 6 151.0 7 303.0 8 612.5 9 753.5 10 658.5 11 631.0 12 684.5 13 809.5 14 920.0 15 1030.0 16 1178.5 17 1309.0 18 1495.5 19 1690.0 20 1920.0 21 2325.0 22 2877.0 23 3536.5 24 4479.0 25 5962.5 26 8413.0 27 12209.0 28 17001.5 29 23477.0 30 31267.0 31 38449.0 32 45383.0 33 52005.5 34 57806.0 35 61803.5 36 66282.0 37 69373.5 38 68605.5 39 68093.0 40 67864.5 41 67915.0 42 68585.0 43 70108.5 44 72195.0 45 73323.0 46 73524.5 47 73672.0 48 73634.5 49 73247.5 50 71843.5 51 69440.0 52 66404.5 53 62365.0 54 58378.5 55 53592.0 56 47359.5 57 40966.0 58 35651.5 59 30807.0 60 25753.5 61 20847.0 62 16235.0 63 12079.5 64 8395.0 65 5789.0 66 4005.0 67 2704.0 68 1919.5 69 1411.0 70 1050.5 71 770.5 72 542.0 73 394.0 74 274.0 75 181.5 76 153.0 77 114.0 78 82.0 79 65.0 80 52.0 81 48.0 82 38.0 83 27.0 84 24.5 85 21.5 86 18.5 87 16.0 88 11.0 89 11.0 90 9.0 91 6.5 92 6.0 93 4.0 94 2.0 95 4.0 96 3.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 9.961101897091856E-5 48-49 1.9922203794183713E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2007810.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.971081515708406 #Duplication Level Percentage of deduplicated Percentage of total 1 68.51017170606016 35.60547718390832 2 15.829937952683967 16.453979914550892 3 6.394657225076533 9.970117557283986 4 3.187305643287291 6.625908856110447 5 1.8461437899519155 4.797304469865493 6 1.117224648234211 3.483802395884329 7 0.7195801178905067 2.617814987477941 8 0.5074814521051967 2.1099487932055396 9 0.3470766275602103 1.6234152932815973 >10 1.4629184549547365 12.37334018185652 >50 0.05507925425680092 1.964486025884947 >100 0.021843554798704708 1.8917648300950267 >500 2.897865698849738E-4 0.09181897908030888 >1k 2.897865698849573E-4 0.3908205315145823 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3697 0.18413096856774297 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2566 0.1278009373396885 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.980550948545928E-5 0.0 5 0.0 0.0 0.0 4.980550948545928E-5 0.0 6 0.0 0.0 0.0 4.980550948545928E-5 0.0 7 0.0 0.0 0.0 4.980550948545928E-5 0.0 8 0.0 0.0 0.0 9.961101897091856E-5 0.0 9 0.0 0.0 0.0 9.961101897091856E-5 0.0 10-11 0.0 0.0 0.0 1.245137737136482E-4 0.0 12-13 0.0 0.0 0.0 1.4941652845637784E-4 0.0 14-15 0.0 0.0 0.0 2.2412479268456677E-4 2.490275474272964E-5 16-17 0.0 0.0 0.0 4.980550948545928E-4 4.980550948545928E-5 18-19 0.0 0.0 0.0 6.474716233109707E-4 4.980550948545928E-5 20-21 0.0 0.0 0.0 9.712074349664561E-4 4.980550948545928E-5 22-23 0.0 0.0 0.0 0.0015439707940492378 7.470826422818892E-5 24-25 0.0 0.0 0.0 0.0029385250596420977 1.7431928319910748E-4 26-27 0.0 0.0 0.0 0.004009343513579473 1.9922203794183713E-4 28-29 0.0 0.0 0.0 0.008168103555615322 1.9922203794183713E-4 30-31 0.0 0.0 0.0 0.021914424173602082 1.9922203794183713E-4 32-33 0.0 0.0 0.0 0.05525921277411707 2.490275474272964E-4 34-35 0.0 0.0 0.0 0.10130440629342419 3.4863856639821497E-4 36-37 0.0 0.0 0.0 0.17939944516662432 3.4863856639821497E-4 38-39 0.0 0.0 0.0 0.30391321888027256 3.4863856639821497E-4 40-41 0.0 0.0 0.0 0.43744178981078885 3.9844407588367426E-4 42-43 0.0 0.0 0.0 0.5696256119851979 3.9844407588367426E-4 44-45 0.0 0.0 0.0 0.7252678291272581 4.2334683062640393E-4 46-47 0.0 0.0 0.0 0.9222735218969923 4.4824958536913355E-4 48-49 0.0 0.0 0.0 1.1301368157345566 4.4824958536913355E-4 50-51 0.0 0.0 0.0 1.3378008875341791 4.4824958536913355E-4 52-53 0.0 0.0 0.0 1.5360019125315643 4.4824958536913355E-4 54-55 0.0 0.0 0.0 1.74999128403584 4.4824958536913355E-4 56-57 0.0 0.0 0.0 2.0017083289753512 4.4824958536913355E-4 58-59 0.0 0.0 0.0 2.2820884446237444 4.7315234011186316E-4 60-61 0.0 0.0 0.0 2.5670506671447995 4.980550948545928E-4 62-63 0.0 0.0 0.0 2.8465591863771973 4.980550948545928E-4 64-65 0.0 0.0 0.0 3.1326420328616753 5.229578495973225E-4 66-67 0.0 0.0 0.0 3.4515965156065564 5.478606043400521E-4 68-69 0.0 0.0 0.0 3.811316807865286 5.727633590827817E-4 70-71 0.0 0.0 0.0 4.173651889372002 5.976661138255114E-4 72-73 0.0 0.0 0.0 4.542287367828629 6.474716233109707E-4 74-75 0.0 0.0 0.0 4.911296387606397 6.474716233109707E-4 76-77 0.0 0.0 0.0 5.306403494354546 6.474716233109707E-4 78-79 0.0 0.0 0.0 5.735378347552807 6.474716233109707E-4 80-81 0.0 0.0 0.0 6.183652835676683 6.972771327964299E-4 82-83 0.0 0.0 0.0 6.650255751291208 7.470826422818892E-4 84-85 0.0 0.0 0.0 7.1240306602716394 7.470826422818892E-4 86-87 0.0 0.0 0.0 7.6269168895463215 7.470826422818892E-4 88-89 0.0 0.0 0.0 8.176794617020535 7.470826422818892E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2215 0.0 57.900677 1 GTATCAA 4350 0.0 44.00575 1 TCAACGC 5105 0.0 36.939274 4 ATCAACG 5195 0.0 36.482193 3 CAACGCA 5260 0.0 35.941063 5 AACGCAG 5455 0.0 34.48213 6 TATCAAC 5575 0.0 34.16592 2 ACGCAGA 6310 0.0 29.734547 7 CGCAGAG 6445 0.0 28.964315 8 GTACATG 5905 0.0 27.188824 1 TACATGG 5960 0.0 26.53943 2 ACATGGG 5955 0.0 25.843828 3 GCAGAGT 7270 0.0 24.63205 9 CATGGGG 3995 0.0 23.660826 4 AGAGTAC 6590 0.0 20.866842 10-11 CAGAGTA 6975 0.0 20.736559 10-11 CCGTGCG 140 4.8534747E-4 20.357143 9 GAGTACT 4185 0.0 19.578854 12-13 TATACTG 1335 0.0 19.56929 5 ATGGGGG 2170 0.0 17.292627 5 >>END_MODULE