FastQCFastQC Report
Fri 10 Feb 2017
SRR2031529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2007810
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.23393647805320222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34380.171231341611009No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT32500.16186790582774266No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20680.1029977936159298No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24950.052.6173971
GTATCAA46100.043.1286771
TCAACGC54050.036.2943534
CAACGCA55150.035.4843145
ATCAACG55850.035.2947123
AACGCAG57250.034.182716
TATCAAC60250.033.032522
ACGCAGA63750.030.6974127
CGCAGAG65400.029.7776818
GTATACG1153.8252856E-628.952831
GTACATG53800.026.0813791
GCAGAGT78400.024.7188849
TACATGG54900.024.4849262
ACATGGG54650.023.7277873
AGAGTAC68100.021.90116510-11
GAGTACT45800.021.83088512-13
CAGAGTA72650.021.37946710-11
CATGGGG34400.021.1260224
GTACTTT52650.018.76504914-15
TACACCG2905.219954E-818.0168745
Produced by FastQC (version 0.11.5)