FastQCFastQC Report
Fri 10 Feb 2017
SRR2031528_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031528_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257176
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32060.25501600412352765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21740.17292725919043953No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16650.060.7657661
GTATCAA32950.044.6889231
TCAACGC38700.038.1718334
ATCAACG39500.036.917723
CAACGCA40850.036.2790685
AACGCAG41800.035.4545446
TATCAAC41700.034.9700242
ACGCAGA45650.032.4644057
CGCAGAG46850.031.632878
GCAGAGT54400.026.6314339
GTACATG38550.024.273671
TACATGG38850.023.4749032
GAGTACT31900.022.63322812-13
CAGAGTA51750.022.62560510-11
ATACGGT1050.002196335522.6190476
ACATGGG38400.022.2656253
AGAGTAC49050.021.4984710-11
GTACTTT34550.020.34732214-15
GTATTAT7400.019.898651
AGTACTT36900.019.24458112-13
Produced by FastQC (version 0.11.5)