##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031528_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1257176 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15968726733568 34.0 31.0 34.0 30.0 34.0 2 32.32188810476815 34.0 31.0 34.0 30.0 34.0 3 32.4213387783413 34.0 31.0 34.0 30.0 34.0 4 35.6573510789261 37.0 35.0 37.0 33.0 37.0 5 35.639387007069814 37.0 35.0 37.0 33.0 37.0 6 35.761897299980276 37.0 37.0 37.0 35.0 37.0 7 35.788280240793654 37.0 37.0 37.0 35.0 37.0 8 35.83447981825934 37.0 37.0 37.0 35.0 37.0 9 37.65588668571465 39.0 39.0 39.0 35.0 39.0 10-11 37.653531406899276 39.0 38.5 39.0 35.0 39.0 12-13 37.61942679465723 39.0 38.5 39.0 35.0 39.0 14-15 39.120238932337244 41.0 39.0 41.0 36.0 41.0 16-17 39.12934227188556 41.0 39.0 41.0 36.0 41.0 18-19 39.08293230223931 41.0 39.0 41.0 36.0 41.0 20-21 39.04065103056374 41.0 39.0 41.0 36.0 41.0 22-23 38.96908030379199 41.0 39.0 41.0 35.5 41.0 24-25 38.91379051143197 41.0 39.0 41.0 35.0 41.0 26-27 38.80022049418697 41.0 39.0 41.0 35.0 41.0 28-29 38.69640925375604 41.0 39.0 41.0 35.0 41.0 30-31 38.526345157718566 40.0 38.5 41.0 34.0 41.0 32-33 38.40874626941653 40.0 38.0 41.0 34.0 41.0 34-35 38.270323327839534 40.0 38.0 41.0 34.0 41.0 36-37 38.13066865737176 40.0 38.0 41.0 33.0 41.0 38-39 37.96977153556861 40.0 38.0 41.0 33.0 41.0 40-41 37.8310157050405 40.0 38.0 41.0 33.0 41.0 42-43 37.66976660388045 40.0 38.0 41.0 32.5 41.0 44-45 37.84747402113945 40.0 38.0 41.0 33.0 41.0 46-47 37.71264126900291 40.0 38.0 41.0 32.5 41.0 48-49 37.572438147085215 40.0 38.0 41.0 32.0 41.0 50-51 36.516766148892444 39.0 36.5 40.0 30.0 40.5 52-53 36.58268929728216 39.0 36.5 40.0 30.5 40.5 54-55 37.36358234646541 40.0 37.0 41.0 31.5 41.0 56-57 37.39849989182103 40.0 37.0 41.0 32.0 41.0 58-59 37.20881244948997 40.0 36.5 41.0 31.0 41.0 60-61 37.039365212189864 40.0 36.0 41.0 31.0 41.0 62-63 36.78386001641775 39.0 35.5 41.0 31.0 41.0 64-65 36.51461569422261 39.0 35.0 41.0 30.0 41.0 66-67 36.22475094974769 39.0 35.0 41.0 30.0 41.0 68-69 35.92859313254469 37.5 35.0 40.5 29.5 41.0 70-71 35.574333665294276 37.0 35.0 40.0 29.0 41.0 72-73 35.220067436858486 37.0 35.0 39.0 29.0 41.0 74-75 34.85044178380752 36.0 35.0 39.0 29.0 41.0 76-77 34.48253824444628 36.0 34.5 39.0 28.5 40.5 78-79 34.09425529917847 35.0 34.0 37.0 28.0 39.5 80-81 33.74676815338505 35.0 34.0 37.0 27.0 39.0 82-83 33.442913720910994 35.0 34.0 36.5 27.0 39.0 84-85 33.15296823992822 35.0 34.0 36.0 26.5 37.0 86-87 32.88678355297906 35.0 34.0 36.0 26.0 37.0 88-89 32.66012197178438 35.0 34.0 35.0 26.0 37.0 90-91 32.48099589874449 35.0 34.0 35.0 25.0 36.0 92-93 32.31892630785188 35.0 33.0 35.0 25.0 36.0 94-95 32.187608576682976 35.0 33.0 35.0 25.0 36.0 96-97 32.06001983811336 35.0 33.0 35.0 25.0 36.0 98-99 31.929234252006083 35.0 33.0 35.0 24.0 35.0 100-101 30.38782437781186 34.0 30.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08849000693786735 1101 2 0.08523992337086383 1101 3 0.2542512967800974 1101 4 -0.10114361448668774 1101 5 0.02147541989313595 1101 6 -0.011027704630052426 1101 7 0.012800943513461505 1101 8 0.05431028097605406 1101 9 0.31474042031914706 1101 10-11 0.465804548280353 1101 12-13 0.5859545103374941 1101 14-15 0.7631950211434742 1101 16-17 0.6260285541400066 1101 18-19 0.7039626928253568 1101 20-21 0.7587314215632404 1101 22-23 0.7716767648807092 1101 24-25 0.7603034662811154 1101 26-27 0.587062369862025 1101 28-29 0.6408967792267006 1101 30-31 0.7477754086206971 1101 32-33 0.8740664719310658 1101 34-35 0.8264832667344493 1101 36-37 0.844454939227731 1101 38-39 0.9875936069799707 1101 40-41 0.8615582823703036 1101 42-43 0.8791667938200973 1101 44-45 0.9638604640472579 1101 46-47 1.0259916721870965 1101 48-49 0.7772848503024932 1101 50-51 0.7863373979646369 1101 52-53 0.9551204382878566 1101 54-55 0.9442055011878168 1101 56-57 0.7791644190072304 1101 58-59 0.8686766406751758 1101 60-61 0.8085604882646962 1101 62-63 0.8257906020834938 1101 64-65 0.944596196300374 1101 66-67 0.7398548770884474 1101 68-69 0.7274311539167542 1101 70-71 0.6347474764664938 1101 72-73 0.6058395614917202 1101 74-75 0.6652324351052741 1101 76-77 0.6196316582068491 1101 78-79 0.8140291613155668 1101 80-81 0.6617541997737746 1101 82-83 0.6927486944920389 1101 84-85 0.6913454154230081 1101 86-87 0.715613977581647 1101 88-89 0.780611739076015 1101 90-91 0.6435383504115535 1101 92-93 0.6009664789926532 1101 94-95 0.659899381765797 1101 96-97 0.7119443521531323 1101 98-99 0.8093412680051308 1101 100-101 0.6478377398714699 1102 1 0.11533587384751343 1102 2 0.19834109367753427 1102 3 0.33457249043595283 1102 4 0.19406980275184083 1102 5 0.021720301581112267 1102 6 0.11986440505416596 1102 7 0.24780763504795544 1102 8 0.29124185647649625 1102 9 0.4161385239097939 1102 10-11 0.5467521834635889 1102 12-13 0.6141415316080341 1102 14-15 0.6367164767657911 1102 16-17 0.6589480564037373 1102 18-19 0.5850042942376987 1102 20-21 0.6972632704199881 1102 22-23 0.6539370798099498 1102 24-25 0.6837637014814391 1102 26-27 0.7214568571765838 1102 28-29 0.6388807764931315 1102 30-31 0.7146508891298211 1102 32-33 0.6337037053374885 1102 34-35 0.7302248311297816 1102 36-37 0.616447308030942 1102 38-39 0.5438309713696157 1102 40-41 0.6363610956622523 1102 42-43 0.6143984348121521 1102 44-45 0.6925856555390411 1102 46-47 0.8469973955846939 1102 48-49 0.7109532349375911 1102 50-51 0.7365267541535943 1102 52-53 0.6259453478809505 1102 54-55 0.5786654801166051 1102 56-57 0.6007538720098751 1102 58-59 0.6644851773647105 1102 60-61 0.5472347101958448 1102 62-63 0.6701882216056916 1102 64-65 0.604287531475066 1102 66-67 0.5896085716697286 1102 68-69 0.5392281868618767 1102 70-71 0.5331557454946534 1102 72-73 0.6012181314965659 1102 74-75 0.6399811466289478 1102 76-77 0.7107959852093302 1102 78-79 0.6495085314570872 1102 80-81 0.3521753393278644 1102 82-83 0.576535248778896 1102 84-85 0.6221976606463997 1102 86-87 0.49875131064921874 1102 88-89 0.6107293392354691 1102 90-91 0.4660703131667603 1102 92-93 0.49276009498399986 1102 94-95 0.5267065231992092 1102 96-97 0.44701938276335085 1102 98-99 0.6572270278502224 1102 100-101 0.6726903837394573 1103 1 0.41922334491404456 1103 2 0.31228166079633013 1103 3 0.49345158392244315 1103 4 0.4102515437362442 1103 5 0.33311700624744844 1103 6 0.33719024498075356 1103 7 0.36854887967786 1103 8 0.3256844392364826 1103 9 0.4037406185330781 1103 10-11 0.4751998886458111 1103 12-13 0.4617129464831464 1103 14-15 0.4849101609378721 1103 16-17 0.48856451389722366 1103 18-19 0.5379697749555632 1103 20-21 0.5641469476069503 1103 22-23 0.47199658573817516 1103 24-25 0.5776021853905462 1103 26-27 0.5481363679441742 1103 28-29 0.45485479001596474 1103 30-31 0.5928605066830173 1103 32-33 0.5690173738044493 1103 34-35 0.6454619828438766 1103 36-37 0.6419863722073131 1103 38-39 0.6163876627048666 1103 40-41 0.7321663705701482 1103 42-43 0.6951733723797773 1103 44-45 0.6883114918132804 1103 46-47 0.7738115867943947 1103 48-49 0.73525278250262 1103 50-51 0.6866625759553884 1103 52-53 0.7239612082150515 1103 54-55 0.7076719949976464 1103 56-57 0.7514316113872468 1103 58-59 0.7592084432969557 1103 60-61 0.7845717811016755 1103 62-63 0.8788064002695677 1103 64-65 0.7120880400649341 1103 66-67 0.7231727640207879 1103 68-69 0.7504180771543929 1103 70-71 0.6582893987130589 1103 72-73 0.5374316479344259 1103 74-75 0.621779655556665 1103 76-77 0.5877985007660627 1103 78-79 0.5737231618619916 1103 80-81 0.6179505279946156 1103 82-83 0.5796456821759932 1103 84-85 0.5776686541315073 1103 86-87 0.6686276329144079 1103 88-89 0.6486923317892419 1103 90-91 0.6919298711409851 1103 92-93 0.8473705032711791 1103 94-95 0.8278214033336155 1103 96-97 0.7518062452568941 1103 98-99 0.5394510356662572 1103 100-101 0.47039716387249797 1104 1 0.18584025503239587 1104 2 0.21869943536636072 1104 3 0.31522116736171313 1104 4 0.30366481561203784 1104 5 0.15687876533279876 1104 6 0.1717847154425911 1104 7 0.11091849428332523 1104 8 0.22073311590366984 1104 9 0.33095608352442696 1104 10-11 0.39133782363970226 1104 12-13 0.44972503568406097 1104 14-15 0.5105427115624224 1104 16-17 0.44978680264376436 1104 18-19 0.601282248812872 1104 20-21 0.5813515044319288 1104 22-23 0.4332016510641452 1104 24-25 0.572915364587935 1104 26-27 0.4864945658822819 1104 28-29 0.5547772846233912 1104 30-31 0.5446652751100274 1104 32-33 0.4907680934973868 1104 34-35 0.4391463185529503 1104 36-37 0.4588009415807903 1104 38-39 0.4324398906773439 1104 40-41 0.3529747231089573 1104 42-43 0.3844407208751832 1104 44-45 0.4973788025812027 1104 46-47 0.6868029668824818 1104 48-49 0.689060388693207 1104 50-51 0.5303473217459 1104 52-53 0.5068468693073456 1104 54-55 0.47658823176227116 1104 56-57 0.4555775347676274 1104 58-59 0.4098424292065559 1104 60-61 0.3868637262819874 1104 62-63 0.5375124292869273 1104 64-65 0.673492815053244 1104 66-67 0.6088814257610125 1104 68-69 0.6545143806802685 1104 70-71 0.620997412524602 1104 72-73 0.5321785046601661 1104 74-75 0.5493211352945409 1104 76-77 0.47217398773777575 1104 78-79 0.4210678845239144 1104 80-81 0.44307293730510366 1104 82-83 0.4133916941339635 1104 84-85 0.34996478214253557 1104 86-87 0.4447842676220546 1104 88-89 0.5290387685915974 1104 90-91 0.5479810329017099 1104 92-93 0.2508053454308339 1104 94-95 0.3070347851112416 1104 96-97 0.39919689704034056 1104 98-99 0.4700349096236316 1104 100-101 0.3492521344696584 1105 1 0.28740194165394684 1105 2 0.24845073094468972 1105 3 0.29947299515662706 1105 4 0.14402936454876425 1105 5 0.14786760043889302 1105 6 0.17129833941020678 1105 7 0.2247980812521746 1105 8 0.22095377212284006 1105 9 0.26574906292725586 1105 10-11 0.2913553052063591 1105 12-13 0.42817337182739124 1105 14-15 0.41032438621881084 1105 16-17 0.42861118670989384 1105 18-19 0.40668133355573843 1105 20-21 0.359159238277023 1105 22-23 0.34449746248076707 1105 24-25 0.3676697291524107 1105 26-27 0.32530908257523805 1105 28-29 0.34339212297717125 1105 30-31 0.4238932891263616 1105 32-33 0.3433068054555548 1105 34-35 0.4999662428771927 1105 36-37 0.5345913026334159 1105 38-39 0.4672258774535152 1105 40-41 0.5022572019174092 1105 42-43 0.5100492736345572 1105 44-45 0.4674996973266303 1105 46-47 0.5160057297469649 1105 48-49 0.5584233604041486 1105 50-51 0.606291520585124 1105 52-53 0.6571696955716462 1105 54-55 0.5591932959778916 1105 56-57 0.7023791850275174 1105 58-59 0.7255529189235119 1105 60-61 0.6571275813947679 1105 62-63 0.560583824005171 1105 64-65 0.6444718604820139 1105 66-67 0.659960232663515 1105 68-69 0.6044219226166092 1105 70-71 0.5964292072210853 1105 72-73 0.5149805278601534 1105 74-75 0.6038298255755663 1105 76-77 0.48070693466373626 1105 78-79 0.43748346409611827 1105 80-81 0.4005134805545012 1105 82-83 0.41661102175406484 1105 84-85 0.4282802583792531 1105 86-87 0.32514469867864193 1105 88-89 0.5129091097540837 1105 90-91 0.5361304731030998 1105 92-93 0.4801044511515826 1105 94-95 0.44261365805521535 1105 96-97 0.4499175260659598 1105 98-99 0.28294096756918563 1105 100-101 0.3211755729772747 1106 1 0.16351378990201937 1106 2 0.17770905380774593 1106 3 0.13840966959073597 1106 4 0.10843041693096467 1106 5 0.11755183443623451 1106 6 0.025018093873917735 1106 7 0.005886740858464634 1106 8 -0.022853727310561567 1106 9 0.14882080432347777 1106 10-11 0.0805176315666003 1106 12-13 0.08147365502001236 1106 14-15 0.08383139048687838 1106 16-17 0.16337867317555066 1106 18-19 0.29718128844735503 1106 20-21 0.2336156456108327 1106 22-23 0.15497991678744683 1106 24-25 0.17312791661697702 1106 26-27 0.138119202695556 1106 28-29 0.24224262940126806 1106 30-31 0.25612238395884646 1106 32-33 0.23014307963529745 1106 34-35 0.28495072632754415 1106 36-37 0.3140677960325533 1106 38-39 0.3423185696757258 1106 40-41 0.32277828806855524 1106 42-43 0.2771414993700958 1106 44-45 0.22896107685094336 1106 46-47 0.1637778524740412 1106 48-49 0.0898026573441868 1106 50-51 0.07216758035703918 1106 52-53 0.09536314524792999 1106 54-55 0.1683955524238101 1106 56-57 0.103231330022723 1106 58-59 0.23944556684416085 1106 60-61 0.05558604831124825 1106 62-63 0.08556286511100808 1106 64-65 0.12395451502589339 1106 66-67 0.04478386622795938 1106 68-69 0.018460751030929146 1106 70-71 0.17382638761720415 1106 72-73 0.15267147750526533 1106 74-75 0.3428532694647757 1106 76-77 0.30436323667131404 1106 78-79 0.26129086084377207 1106 80-81 0.030621454616074573 1106 82-83 0.07854314207534685 1106 84-85 0.2683244719274711 1106 86-87 0.1788365812971051 1106 88-89 0.18406497718091686 1106 90-91 0.19776737190556304 1106 92-93 0.08316426633190588 1106 94-95 0.015419685415096751 1106 96-97 0.11641989553954346 1106 98-99 0.03244092466121984 1106 100-101 0.0495578397357761 1107 1 0.18540605786512288 1107 2 0.20303953487887583 1107 3 0.2506899634906645 1107 4 0.1148344117294613 1107 5 0.20333858229313506 1107 6 0.19530480876257883 1107 7 0.10017589471679855 1107 8 0.10662782849107089 1107 9 0.22194163188736837 1107 10-11 0.07955509503813829 1107 12-13 0.20682772367415936 1107 14-15 0.2110021108870086 1107 16-17 0.12813562930332267 1107 18-19 0.15811311353489543 1107 20-21 0.25844922399672754 1107 22-23 0.2665012615107969 1107 24-25 0.24214029024157213 1107 26-27 0.4120495533892097 1107 28-29 0.37256028684838327 1107 30-31 0.37122978903241943 1107 32-33 0.22751139872431025 1107 34-35 0.38952291078924617 1107 36-37 0.3746491381864203 1107 38-39 0.3867390186827038 1107 40-41 0.5013556645175399 1107 42-43 0.45095785474185135 1107 44-45 0.3221507230575966 1107 46-47 0.3923207992089317 1107 48-49 0.41546288874721427 1107 50-51 0.36232849933470135 1107 52-53 0.3367042250970016 1107 54-55 0.3394514128505719 1107 56-57 0.2641030645529767 1107 58-59 0.35820428898175294 1107 60-61 0.32920371058464326 1107 62-63 0.19785539464476898 1107 64-65 0.40082923505080004 1107 66-67 0.3728898648834047 1107 68-69 0.2951910226008181 1107 70-71 0.2724904603295002 1107 72-73 0.19859554995925066 1107 74-75 0.33039467518955945 1107 76-77 0.3935810601831733 1107 78-79 0.2495225602441593 1107 80-81 0.34850220205016313 1107 82-83 0.36362355398117074 1107 84-85 0.508398144038317 1107 86-87 0.4696303544470908 1107 88-89 0.3743695289046727 1107 90-91 0.44222378008087304 1107 92-93 0.4573867087955392 1107 94-95 0.3535504068484556 1107 96-97 0.5098386879776413 1107 98-99 0.5476589942452641 1107 100-101 0.4739297268715674 1108 1 0.14079125247049973 1108 2 0.049916302750737884 1108 3 7.19815444547578E-4 1108 4 -0.05537457804778967 1108 5 0.15493143687724853 1108 6 0.15077287108692872 1108 7 0.14797947603776151 1108 8 0.13032995362393507 1108 9 0.22789244761239758 1108 10-11 0.05618052577083432 1108 12-13 0.19341750823766546 1108 14-15 0.13890458536928918 1108 16-17 0.0796606237081221 1108 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-0.7545080849277994 2316 72-73 -0.6372502966270304 2316 74-75 -0.6244224957057227 2316 76-77 -0.5262170018036585 2316 78-79 -0.5255907370904609 2316 80-81 -0.42336578621326737 2316 82-83 -0.3721138033184346 2316 84-85 -0.41958469492906403 2316 86-87 -0.3845506747217371 2316 88-89 -0.45165310590559216 2316 90-91 -0.33611658039944103 2316 92-93 -0.3027452043144052 2316 94-95 -0.33470562786811087 2316 96-97 -0.37647218314201325 2316 98-99 -0.2626923631036462 2316 100-101 -0.3297502790460314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 31.0 7 418.0 8 703.0 9 549.0 10 644.0 11 1088.0 12 1550.0 13 1829.0 14 1941.0 15 2156.0 16 2672.0 17 3182.0 18 3812.0 19 4823.0 20 6044.0 21 7805.0 22 9062.0 23 9125.0 24 8893.0 25 8125.0 26 8121.0 27 8881.0 28 10432.0 29 11917.0 30 14229.0 31 17807.0 32 22657.0 33 30112.0 34 43578.0 35 69609.0 36 130108.0 37 267844.0 38 396967.0 39 150229.0 40 233.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83314348985345 19.784819309309118 13.89407688342762 23.487960317409815 2 14.402438481167316 21.1722145507073 41.377261417653536 23.04808555047185 3 17.225352695247125 27.36999433651295 31.172803171552747 24.23184979668718 4 11.752371982920451 18.219008317053458 40.659223529561494 29.369396170464597 5 12.643893933705384 38.58528956963862 35.87325879590447 12.897557700751525 6 28.44510235639242 39.480072798080776 19.25617415540863 12.818650690118169 7 25.52005447129121 33.66815783947514 23.9917084004149 16.82007928881875 8 24.86079912438672 35.97117666897873 21.46294552234532 17.705078684289234 9 26.240240030035572 16.278229937574373 20.938038906247016 36.54349112614304 10-11 23.477858311008166 26.74951637638644 30.128876147810647 19.643749164794748 12-13 24.835941825170064 24.62638484985396 29.55699917911255 20.980674145863425 14-15 22.076344123654923 25.77586590898967 27.267303861989095 24.880486105366312 16-17 21.496711677601226 28.990570930402743 27.53393319630664 21.978784195689386 18-19 21.599322608767586 28.073793963613685 29.367845074993475 20.95903835262525 20-21 22.501702227850355 27.321791061872002 29.18843503216733 20.988071678110305 22-23 22.194505781211223 27.13585846373141 28.906493601532325 21.763142153525045 24-25 21.856923772009647 27.376556663506147 28.96638974972478 21.80012981475943 26-27 21.863525870681592 27.539262601258695 28.842381655392725 21.754829872666992 28-29 21.82614049265974 27.425714458437007 29.042751372918353 21.705393675984904 30-31 22.125382603549543 27.393022138507256 28.864773110527086 21.616822147416116 32-33 21.882815134873717 27.501240876376897 28.88740319573393 21.72854079301546 34-35 22.05912298675762 27.469304218343332 28.77496865991715 21.696604134981897 36-37 22.266492519742663 27.358619636391406 28.647977689679088 21.726910154186843 38-39 22.071332892132844 27.613635640514932 28.60474587488148 21.710285592470743 40-41 22.114803337002932 27.47017919527576 28.72517451812634 21.689842949594965 42-43 22.09642882142198 27.46437253017875 28.65187531419626 21.787323334203005 44-45 22.122359955964797 27.651697137075477 28.356888772932347 21.869054134027376 46-47 22.10478505950054 27.57658350259531 28.22541995993393 22.093211477970222 48-49 22.076118402161036 27.659836792549637 28.265322274659095 21.998722530630232 50-51 22.05379358180557 27.844152290530523 28.044959496522363 22.057094631141545 52-53 22.21033490935239 27.856879227729454 27.92942276976334 22.003363093154817 54-55 22.13858680089343 27.748541174823572 27.997909600565073 22.11496242371792 56-57 22.068707961335566 27.876168491921575 28.00260265865718 22.052520888085677 58-59 22.09157667661489 27.781869841613265 27.94735979687808 22.17919368489376 60-61 22.111104570879494 27.76023404837509 28.067231636620487 22.06142974412493 62-63 22.027981762298992 27.665219507849336 28.103304549243703 22.203494180607965 64-65 22.03502134943715 27.58193761255385 28.053271777380413 22.329769260628584 66-67 22.046236962843707 27.678861193659436 27.922780899412654 22.352120944084202 68-69 22.098258318644326 27.645612070227237 27.90957670206876 22.346552909059668 70-71 22.106212654393655 27.479644854817465 27.962075318014346 22.452067172774537 72-73 22.069423851553005 27.557796203554634 27.884759174530853 22.48802077036151 74-75 22.063259241347275 27.563284695221675 27.810624765347097 22.56283129808396 76-77 22.163483871788834 27.45721362800435 27.823630104297248 22.55567239590956 78-79 22.115360140505384 27.676793066364613 27.623220615092876 22.584626178037123 80-81 22.17127912082318 27.573426473302064 27.628351161651192 22.62694324422356 82-83 22.210454224388627 27.5818978408751 27.65579361998638 22.551854314749882 84-85 22.217613126563027 27.510149732416146 27.620357054223117 22.651880086797714 86-87 22.195738703252367 27.46401458507003 27.713263695775293 22.626983015902308 88-89 22.364688794568146 27.340046262416717 27.680213430736828 22.61505151227831 90-91 22.321854696558 27.303297231254813 27.78131303811081 22.593535034076375 92-93 22.38620527277008 27.325967088140402 27.613993585623653 22.673834053465864 94-95 22.381870159786697 27.236719441032918 27.71362164088401 22.66778875829637 96-97 22.412812525851592 27.056474192953097 27.79225024976614 22.73846303142917 98-99 22.41543745664887 27.108614863789953 27.65356640597657 22.822381273584604 100-101 22.767615671950466 26.9512781026682 27.583647794739957 22.697458430641372 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 23.0 1 21.5 2 39.5 3 74.0 4 82.0 5 90.0 6 128.5 7 226.0 8 485.0 9 646.5 10 599.5 11 579.5 12 623.5 13 679.5 14 766.5 15 896.0 16 995.5 17 1111.0 18 1276.5 19 1428.5 20 1600.0 21 1856.0 22 2262.5 23 2834.0 24 3556.5 25 4670.5 26 6407.0 27 8940.0 28 12041.5 29 15460.0 30 19143.5 31 22942.0 32 26843.5 33 30246.0 34 33652.5 35 36001.0 36 37677.5 37 39418.0 38 40011.0 39 40721.5 40 41192.0 41 41128.0 42 40931.5 43 41472.5 44 42533.5 45 43225.5 46 43751.5 47 44049.0 48 44737.0 49 44799.5 50 43686.5 51 42741.0 52 41687.5 53 39810.5 54 37501.0 55 34914.0 56 31742.0 57 28215.0 58 25035.5 59 21905.0 60 18621.5 61 15386.0 62 12322.5 63 9442.0 64 7025.5 65 5354.5 66 3961.5 67 2970.5 68 2322.5 69 1784.0 70 1365.0 71 1045.0 72 841.0 73 662.5 74 506.0 75 376.5 76 274.5 77 208.0 78 150.0 79 103.5 80 77.5 81 61.0 82 47.5 83 38.0 84 27.5 85 24.0 86 21.0 87 13.0 88 7.5 89 5.5 90 5.5 91 5.0 92 3.5 93 3.5 94 4.0 95 2.5 96 1.5 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.9885839373325612E-4 48-49 2.3863007247990734E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1257176.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.80574288736121 #Duplication Level Percentage of deduplicated Percentage of total 1 72.89802249434412 44.32618412788163 2 15.172206364687021 18.45114558490289 3 5.418203068444195 9.88373588073988 4 2.539985330474317 6.177827797699621 5 1.3650892584399812 4.1502633233500035 6 0.7812743063758216 2.850357875879381 7 0.48087603876438956 2.046801734166016 8 0.31544073090496516 1.5344486383686886 9 0.21860399624217852 1.1963140550686426 >10 0.7829696891001279 7.385783041494204 >50 0.019952464427077838 0.8200255825480958 >100 0.006981106509013027 0.6461854165489812 >500 0.0 0.0 >1k 3.9515128689441724E-4 0.5309269413520032 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3206 0.25501600412352765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2174 0.17292725919043953 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.954335749330245E-5 0.0 5 0.0 0.0 0.0 7.954335749330245E-5 0.0 6 0.0 0.0 0.0 7.954335749330245E-5 0.0 7 0.0 0.0 0.0 7.954335749330245E-5 0.0 8 0.0 0.0 0.0 7.954335749330245E-5 0.0 9 0.0 0.0 0.0 7.954335749330245E-5 0.0 10-11 0.0 0.0 0.0 7.954335749330245E-5 0.0 12-13 0.0 0.0 0.0 1.590867149866049E-4 1.1931503623995368E-4 14-15 0.0 0.0 0.0 2.7840175122655856E-4 2.7840175122655856E-4 16-17 0.0 0.0 0.0 5.170318237064659E-4 5.568035024531171E-4 18-19 0.0 0.0 0.0 7.15890217439722E-4 7.158902174397221E-4 20-21 0.0 0.0 0.0 0.0011136070049062342 0.0010340636474129319 22-23 0.0 0.0 0.0 0.00226698568855912 0.0011136070049062342 24-25 0.0 0.0 0.0 0.0034203643722120055 0.0011136070049062342 26-27 0.0 0.0 0.0 0.004494199698371589 0.0011136070049062342 28-29 0.0 0.0 0.0 0.006999815459410616 0.0011136070049062342 30-31 0.0 0.0 0.0 0.01618707324988705 0.0011136070049062342 32-33 0.0 0.0 0.0 0.03798195320305192 0.0011136070049062342 34-35 0.0 0.0 0.0 0.07262308539138514 0.0011136070049062342 36-37 0.0 0.0 0.0 0.12699097023805736 0.0011533786836528856 38-39 0.0 0.0 0.0 0.2125796229008508 0.0011931503623995368 40-41 0.0 0.0 0.0 0.31511101070971764 0.0012726937198928392 42-43 0.0 0.0 0.0 0.41092098481040046 0.0012726937198928392 44-45 0.0 0.0 0.0 0.5200544712912114 0.0012726937198928392 46-47 0.0 0.0 0.0 0.6581815115783312 0.0012726937198928392 48-49 0.0 0.0 0.0 0.8099104659968055 0.0012726937198928392 50-51 0.0 0.0 0.0 0.9644234379275456 0.0012726937198928392 52-53 0.0 0.0 0.0 1.1115786492901552 0.0012726937198928392 54-55 0.0 0.0 0.0 1.2654552743609486 0.0013124653986394904 56-57 0.0 0.0 0.0 1.4501151787816502 0.0013522370773861416 58-59 0.0 0.0 0.0 1.6612630212476216 0.0013920087561327928 60-61 0.0 0.0 0.0 1.882115153327776 0.001431780434879444 62-63 0.0 0.0 0.0 2.0954504381248134 0.0015113237923727466 64-65 0.0 0.0 0.0 2.3061210204458247 0.0015113237923727466 66-67 0.0 0.0 0.0 2.5407739250510666 0.0015113237923727466 68-69 0.0 0.0 0.0 2.8192949913138654 0.0015113237923727466 70-71 0.0 0.0 0.0 3.1129292955003915 0.0015113237923727466 72-73 0.0 0.0 0.0 3.4000012726937197 0.0015510954711193978 74-75 0.0 0.0 0.0 3.6823801917949437 0.001590867149866049 76-77 0.0 0.0 0.0 3.974781573940324 0.001590867149866049 78-79 0.0 0.0 0.0 4.296971943466945 0.001749953864852654 80-81 0.0 0.0 0.0 4.648672898623582 0.0018692689010926075 82-83 0.0 0.0 0.0 5.010237230109388 0.0019885839373325613 84-85 0.0 0.0 0.0 5.375500327718633 0.0019885839373325613 86-87 0.0 0.0 0.0 5.771904649786506 0.0020681272948258637 88-89 0.0 0.0 0.0 6.208040878922283 0.0021874423310658175 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1665 0.0 60.765766 1 GTATCAA 3295 0.0 44.688923 1 TCAACGC 3870 0.0 38.171833 4 ATCAACG 3950 0.0 36.91772 3 CAACGCA 4085 0.0 36.279068 5 AACGCAG 4180 0.0 35.454544 6 TATCAAC 4170 0.0 34.970024 2 ACGCAGA 4565 0.0 32.464405 7 CGCAGAG 4685 0.0 31.63287 8 GCAGAGT 5440 0.0 26.631433 9 GTACATG 3855 0.0 24.27367 1 TACATGG 3885 0.0 23.474903 2 GAGTACT 3190 0.0 22.633228 12-13 CAGAGTA 5175 0.0 22.625605 10-11 ATACGGT 105 0.0021963355 22.619047 6 ACATGGG 3840 0.0 22.265625 3 AGAGTAC 4905 0.0 21.49847 10-11 GTACTTT 3455 0.0 20.347322 14-15 GTATTAT 740 0.0 19.89865 1 AGTACTT 3690 0.0 19.244581 12-13 >>END_MODULE