FastQCFastQC Report
Fri 10 Feb 2017
SRR2031528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1257176
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39750.31618484603587727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28940.23019847658561726No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT17150.1364168581010137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16330.1298943027865629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.057.3114931
GTATCAA32850.047.197591
TCAACGC39600.038.622934
ATCAACG40150.037.9755443
CAACGCA40900.037.511445
AACGCAG41550.036.924626
TATCAAC41900.036.502822
GTACGGG851.618389E-533.5714381
ACGCAGA46000.033.3525627
CGCAGAG47100.032.573638
GCAGAGT56250.026.9372149
GTACATG33650.024.168441
AGAGTAC49500.022.9818410-11
TACATGG34850.022.8976552
GAGTACT36250.022.53745812-13
CAGAGTA53300.022.18996610-11
ACATGGG34650.021.2477513
AGTACTT40600.020.64919912-13
GTACTTT40800.019.84946314-15
ACTATAC3951.8189894E-1219.2401053
Produced by FastQC (version 0.11.5)