FastQCFastQC Report
Fri 10 Feb 2017
SRR2031527_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031527_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690931
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30910.18279870674793947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22480.1329445139985014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19200.050.468641
GTATCAA36350.043.2531
TCAACGC40750.037.4170954
CAACGCA41500.036.96985
ATCAACG41900.036.2767683
AACGCAG43950.034.692766
TATCAAC44900.034.3818862
ACGCAGA48400.031.503037
CGCAGAG49350.030.8965918
GTACATG45050.026.9921721
GCAGAGT55500.026.5314759
TACATGG45450.026.2320652
ACATGGG44850.025.4180073
CATGGGG27650.021.8173124
GAGTACT35500.021.7429112-13
AGAGTAC50750.021.6674410-11
CAGAGTA55900.020.64843410-11
GTACTTT39650.019.16767714-15
AGTACTT39350.018.83096112-13
GTATTAC5500.016.4090561
Produced by FastQC (version 0.11.5)