FastQCFastQC Report
Fri 10 Feb 2017
SRR2031527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690931
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40950.24217428150527728No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT36700.2170401985651691No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29700.17564288548734397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18630.11017599180569757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.052.4446371
GTATCAA38800.046.3401341
TCAACGC44300.039.7786984
CAACGCA44400.039.6891065
ATCAACG44800.039.2287143
AACGCAG45900.038.5990376
TATCAAC47600.037.320292
ACGCAGA51100.034.6711547
CGCAGAG52100.033.7321788
GCAGAGT62750.027.780039
GTACATG39100.024.8170781
TACATGG39100.023.8100172
ACATGGG36650.023.1984733
AGAGTAC56100.022.94493310-11
CAGAGTA61100.021.49484410-11
GAGTACT43400.020.90374212-13
CATGGGG22850.019.9556274
ATGACCG1200.004765251719.7910615
ACCCGTC1200.004765251719.7910617
AGTACTT47100.019.46331212-13
Produced by FastQC (version 0.11.5)