FastQCFastQC Report
Fri 10 Feb 2017
SRR2031526_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031526_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884992
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55440.6264463407578825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44150.4988745660977727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24390.27559571159965285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28350.055.2911
GTATCAA50150.041.8644071
AACGCAG58350.035.7369356
TCAACGC60300.034.817584
ATCAACG60050.034.804333
CAACGCA60250.034.6887975
TATCAAC60900.034.5525442
ACGCAGA65950.031.618657
CGCAGAG66050.031.4988658
GCAGAGT72400.028.1457189
GTACATG50150.028.0358921
TACATGG51350.027.2882182
ACATGGG49900.026.4629293
GAGTACT42250.025.97041312-13
CATGGGG20050.025.5860354
CAGAGTA71100.023.88361510-11
GTACTTT46450.023.36652414-15
ACGATAC1503.0321962E-522.1666663
AGAGTAC67200.022.05357210-11
CGATACT1100.002876848721.590914
Produced by FastQC (version 0.11.5)