##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031526_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 884992 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11389142500723 34.0 31.0 34.0 30.0 34.0 2 32.25694921536014 34.0 31.0 34.0 30.0 34.0 3 32.341008732282326 34.0 31.0 34.0 30.0 34.0 4 35.57115770538039 37.0 35.0 37.0 35.0 37.0 5 35.58190469518369 37.0 35.0 37.0 35.0 37.0 6 35.71901553912352 37.0 37.0 37.0 35.0 37.0 7 35.74559205054961 37.0 37.0 37.0 35.0 37.0 8 35.793598134220424 37.0 37.0 37.0 35.0 37.0 9 37.59634324378074 39.0 39.0 39.0 35.0 39.0 10-11 37.603698677502166 39.0 39.0 39.0 35.0 39.0 12-13 37.57835438060457 39.0 39.0 39.0 35.0 39.0 14-15 39.0481478928623 41.0 39.0 41.0 36.0 41.0 16-17 39.0622886986549 41.0 39.0 41.0 36.0 41.0 18-19 39.06863112886896 41.0 39.5 41.0 36.0 41.0 20-21 39.05457506960515 41.0 40.0 41.0 36.0 41.0 22-23 38.98067270664593 41.0 39.0 41.0 36.0 41.0 24-25 38.91996594319497 41.0 39.0 41.0 35.0 41.0 26-27 38.81557686397165 41.0 39.0 41.0 35.0 41.0 28-29 38.69839049392537 41.0 39.0 41.0 35.0 41.0 30-31 38.47752804545126 40.5 38.5 41.0 35.0 41.0 32-33 38.296242790895285 40.0 38.0 41.0 34.0 41.0 34-35 38.13733513975268 40.0 38.0 41.0 34.0 41.0 36-37 37.97057713516054 40.0 38.0 41.0 33.5 41.0 38-39 37.788617298235465 40.0 38.0 41.0 33.0 41.0 40-41 37.64409339293462 40.0 38.0 41.0 33.0 41.0 42-43 37.44267349309372 40.0 38.0 41.0 32.5 41.0 44-45 37.46690252567255 40.0 38.0 41.0 32.5 41.0 46-47 37.1958972510486 40.0 38.0 41.0 30.5 41.0 48-49 36.955344794077234 40.0 38.0 41.0 29.5 41.0 50-51 35.89118206718253 39.0 36.5 40.0 27.0 40.5 52-53 35.93055191459358 39.5 36.5 40.0 27.5 40.5 54-55 36.67287444406277 40.0 37.0 41.0 28.0 41.0 56-57 36.714246004483655 40.0 37.0 41.0 28.0 41.0 58-59 36.54672019634076 40.0 37.0 41.0 27.0 41.0 60-61 36.40306240056407 40.0 36.5 41.0 26.5 41.0 62-63 36.16106077795054 40.0 36.0 41.0 26.0 41.0 64-65 35.90538219554527 39.5 35.5 41.0 26.0 41.0 66-67 35.62804522526757 39.0 35.0 41.0 25.0 41.0 68-69 35.33102615616865 39.0 35.0 41.0 25.0 41.0 70-71 34.97693707965722 37.5 35.0 40.5 24.0 41.0 72-73 34.595631937915826 37.0 35.0 40.0 23.0 41.0 74-75 34.1810886426092 37.0 35.0 39.0 22.0 41.0 76-77 33.77326122722013 36.0 34.5 39.0 21.5 41.0 78-79 33.33272560655915 36.0 34.0 38.0 20.0 40.0 80-81 32.94427181262655 35.0 34.0 37.0 20.0 39.0 82-83 32.588001360464276 35.0 34.0 37.0 19.0 39.0 84-85 32.241552466010994 35.0 34.0 36.0 18.5 38.0 86-87 31.929801625325425 35.0 34.0 36.0 18.0 37.0 88-89 31.657477129736765 35.0 33.0 36.0 16.5 37.0 90-91 31.42081171355221 35.0 33.0 35.0 15.0 36.5 92-93 31.209902462395142 35.0 33.0 35.0 13.5 36.0 94-95 31.027082165714493 35.0 33.0 35.0 11.5 36.0 96-97 30.86351797530373 35.0 32.5 35.0 10.5 36.0 98-99 30.690687599435925 35.0 32.0 35.0 10.0 35.5 100-101 29.155411009365057 33.5 28.5 34.5 8.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.059970137146891034 1101 2 0.04003202222050106 1101 3 0.17125661825680538 1101 4 0.1526759258594339 1101 5 -0.10610161954562614 1101 6 -0.016076131672086547 1101 7 -0.004608801613436242 1101 8 0.006972511016151373 1101 9 0.1535238433718078 1101 10-11 0.1572468706426733 1101 12-13 0.24682879790557877 1101 14-15 0.2895220257541098 1101 16-17 0.12980110570372716 1101 18-19 0.14004740757464873 1101 20-21 0.2626713037282187 1101 22-23 0.1283199325887665 1101 24-25 0.12594871856045842 1101 26-27 0.21483436220530194 1101 28-29 0.18088174986348093 1101 30-31 0.2503476197334251 1101 32-33 0.2712627967195971 1101 34-35 0.09149786106773661 1101 36-37 0.18783025970048328 1101 38-39 0.21109621359350683 1101 40-41 0.11392199051316965 1101 42-43 0.1702384810081483 1101 44-45 0.19198216540294766 1101 46-47 0.11980531221736612 1101 48-49 0.19581082264232919 1101 50-51 0.09116252330147745 1101 52-53 0.32637799415929436 1101 54-55 0.31631305857015235 1101 56-57 0.1757007205519301 1101 58-59 0.03294712073272166 1101 60-61 0.17096606946915216 1101 62-63 0.07938334989001561 1101 64-65 0.1054291156828242 1101 66-67 0.1809086352307574 1101 68-69 0.2849165372164819 1101 70-71 0.4073288010550584 1101 72-73 0.2737277160261087 1101 74-75 0.2460004067754582 1101 76-77 0.18331810861005948 1101 78-79 0.34921983127341605 1101 80-81 0.3588876631173079 1101 82-83 0.3905428733208751 1101 84-85 0.3386995527372818 1101 86-87 0.11921427682409558 1101 88-89 0.1402313090202334 1101 90-91 0.28608587580890443 1101 92-93 0.5574847295649406 1101 94-95 0.34372702686802015 1101 96-97 0.40420432825500185 1101 98-99 0.43954257553942 1101 100-101 0.29303165846541646 1102 1 0.05413895272238278 1102 2 0.21563629200523593 1102 3 0.33011966394877135 1102 4 0.1399952528667896 1102 5 -0.004478918962504963 1102 6 0.16637002739614104 1102 7 0.17078775439075855 1102 8 0.1869033068639041 1102 9 0.31761013071596267 1102 10-11 0.32912242475718045 1102 12-13 0.3258740237816511 1102 14-15 0.29476426561793545 1102 16-17 0.3081892467667231 1102 18-19 0.34206552426926606 1102 20-21 0.33958746335373036 1102 22-23 0.4674202786095307 1102 24-25 0.4591165563613302 1102 26-27 0.37137455988803225 1102 28-29 0.35616811624019107 1102 30-31 0.518121079991694 1102 32-33 0.45855223320730687 1102 34-35 0.5335013871358143 1102 36-37 0.7966038871430143 1102 38-39 0.5951019991822264 1102 40-41 0.5183293223030105 1102 42-43 0.6174842469400517 1102 44-45 0.8214549776955451 1102 46-47 0.8030734605756606 1102 48-49 0.7721747675829747 1102 50-51 0.7166875072163279 1102 52-53 0.8507818302781871 1102 54-55 0.814657681063018 1102 56-57 0.7623452310820014 1102 58-59 1.0860091258293139 1102 60-61 1.220698321339846 1102 62-63 0.9652889566422331 1102 64-65 0.8880148482791981 1102 66-67 0.7823134896537951 1102 68-69 0.8172160300908899 1102 70-71 0.8188085337628834 1102 72-73 0.851516526839383 1102 74-75 0.7422716911052234 1102 76-77 0.7287732297679881 1102 78-79 0.608466894149899 1102 80-81 0.5708342452123745 1102 82-83 0.5834114512920721 1102 84-85 0.6652851434803466 1102 86-87 0.66335345518943 1102 88-89 0.678012419225059 1102 90-91 0.7053270112433587 1102 92-93 0.5538649369747475 1102 94-95 0.3300039853303325 1102 96-97 0.28205722636885966 1102 98-99 0.2584148923586618 1102 100-101 0.01617705304560957 1103 1 0.19842584174944022 1103 2 0.25939970482939856 1103 3 0.3523724167115603 1103 4 0.1280515716858588 1103 5 0.04501465291017581 1103 6 0.1549718846335537 1103 7 0.19312073339154523 1103 8 0.12388960823376749 1103 9 0.19516483498198767 1103 10-11 0.1336060686329077 1103 12-13 0.26840295536872105 1103 14-15 0.3066287233888474 1103 16-17 0.21887538166713938 1103 18-19 0.1751969549036545 1103 20-21 0.301838009894972 1103 22-23 0.3071463060067927 1103 24-25 0.2359833206365849 1103 26-27 0.22490451435964331 1103 28-29 0.11673992875147121 1103 30-31 0.3053270006880595 1103 32-33 0.34654838234788343 1103 34-35 0.4635270595319696 1103 36-37 0.4674576948808422 1103 38-39 0.5085057978945926 1103 40-41 0.48314510280402345 1103 42-43 0.5319771168331542 1103 44-45 0.6339762877900199 1103 46-47 0.6262066963004074 1103 48-49 0.7145883739529424 1103 50-51 0.7514104258468635 1103 52-53 0.8139188767492413 1103 54-55 0.6750820778619939 1103 56-57 0.6408355738486051 1103 58-59 0.6075548848698062 1103 60-61 0.5969413266147185 1103 62-63 0.6678200944508532 1103 64-65 0.7586831328416608 1103 66-67 0.5036346152279094 1103 68-69 0.5133471197637647 1103 70-71 0.65187778906283 1103 72-73 0.6395427608192037 1103 74-75 0.5447727440745993 1103 76-77 0.4776229860807959 1103 78-79 0.647269316982289 1103 80-81 0.6583259718815881 1103 82-83 0.5463138360169992 1103 84-85 0.7237590398681562 1103 86-87 0.6337589988224721 1103 88-89 0.717462668929226 1103 90-91 0.719901110202418 1103 92-93 0.7303837199427186 1103 94-95 0.6949380995323828 1103 96-97 0.6988054563774853 1103 98-99 0.6771687783170641 1103 100-101 0.668805465167793 1104 1 0.2553918756612461 1104 2 0.2139245958013305 1104 3 0.33200543095306756 1104 4 0.2283845370683153 1104 5 0.22949738033096168 1104 6 0.2764988204472374 1104 7 0.24767095868605793 1104 8 0.33881876261027344 1104 9 0.4855323636188231 1104 10-11 0.46849414521494737 1104 12-13 0.40712737222690265 1104 14-15 0.5545078786701438 1104 16-17 0.5985083959086452 1104 18-19 0.5727847654753049 1104 20-21 0.5296089514269724 1104 22-23 0.4647782706835031 1104 24-25 0.5914654870830844 1104 26-27 0.6078536104672025 1104 28-29 0.5846621523811564 1104 30-31 0.6354477006391974 1104 32-33 0.4616313522543294 1104 34-35 0.39826674936747963 1104 36-37 0.6294272575018525 1104 38-39 0.653832520176131 1104 40-41 0.46676374886543925 1104 42-43 0.39522968193330854 1104 44-45 0.520207083468712 1104 46-47 0.5333401177006749 1104 48-49 0.38234321009415595 1104 50-51 0.4942172878290947 1104 52-53 0.5615989766388481 1104 54-55 0.6930585893257799 1104 56-57 0.7194561199842155 1104 58-59 0.7790275328493905 1104 60-61 0.6844732207172441 1104 62-63 0.5889217755641099 1104 64-65 0.548727648865615 1104 66-67 0.6393152941877602 1104 68-69 0.6736482362835545 1104 70-71 0.749396835044621 1104 72-73 0.7129706756235024 1104 74-75 0.6918095331301686 1104 76-77 0.6618197975753404 1104 78-79 0.5738567837309887 1104 80-81 0.6376377831046867 1104 82-83 0.5954687553995583 1104 84-85 0.47856713237462856 1104 86-87 0.5035487239450731 1104 88-89 0.631569362619171 1104 90-91 0.6991658719693099 1104 92-93 0.5924844781628025 1104 94-95 0.4304070559392521 1104 96-97 0.508194537284453 1104 98-99 0.5563177260378787 1104 100-101 0.6154206187118803 1105 1 0.08929696135582788 1105 2 0.2186931811665218 1105 3 0.20296177638024204 1105 4 0.12799847622021332 1105 5 0.21391840852944455 1105 6 0.05921244248990831 1105 7 0.07698325153249641 1105 8 0.16235550324633152 1105 9 0.3344873368403327 1105 10-11 0.3032372321315435 1105 12-13 0.3387031217345111 1105 14-15 0.29604679708534576 1105 16-17 0.2026688757027486 1105 18-19 0.2849134251170966 1105 20-21 0.3321325127444297 1105 22-23 0.3278853948886038 1105 24-25 0.2756474033239229 1105 26-27 0.21166250522721697 1105 28-29 0.3532483709305083 1105 30-31 0.15051124195385768 1105 32-33 0.18187633534069647 1105 34-35 0.2886447544808206 1105 36-37 0.17053369219066639 1105 38-39 0.401081281948251 1105 40-41 0.27450647054321564 1105 42-43 0.14716595970596558 1105 44-45 0.3542162792947394 1105 46-47 0.27594829836759516 1105 48-49 0.15889300033784792 1105 50-51 0.3171780092952403 1105 52-53 0.27299289182834485 1105 54-55 0.08520561331206267 1105 56-57 0.2781546068031915 1105 58-59 0.31156536237502763 1105 60-61 0.19341047814917545 1105 62-63 0.0547975465764452 1105 64-65 0.0879157263781778 1105 66-67 -0.013555926699808651 1105 68-69 0.18278006019065174 1105 70-71 0.10544580435431783 1105 72-73 0.05569440146908988 1105 74-75 0.06345880828471451 1105 76-77 -0.052919295608354844 1105 78-79 0.004589411846183111 1105 80-81 0.005508372504571923 1105 82-83 0.08661683414095478 1105 84-85 0.049347514612414045 1105 86-87 -0.05915629621132723 1105 88-89 -0.039999013843065256 1105 90-91 0.23092372887078483 1105 92-93 0.4228831004956213 1105 94-95 0.2453256964316104 1105 96-97 0.20584591512231043 1105 98-99 0.021852152356924393 1105 100-101 0.2926382498479825 1106 1 0.20953601907526576 1106 2 0.24049055279981957 1106 3 0.20374648175904042 1106 4 0.22870708895621306 1106 5 0.13874521850392085 1106 6 0.08801667831369286 1106 7 0.038611951140936185 1106 8 0.09487876698660358 1106 9 0.12662125989164252 1106 10-11 -0.01741082354290313 1106 12-13 0.1751388155250453 1106 14-15 0.2125493889910004 1106 16-17 0.19724331174529652 1106 18-19 0.17364220240344252 1106 20-21 0.20015299434744804 1106 22-23 0.23245095345615852 1106 24-25 0.18935755376541152 1106 26-27 0.29704082833560363 1106 28-29 0.31576085487971994 1106 30-31 0.49594806128138913 1106 32-33 0.5092847467482571 1106 34-35 0.3901721557585489 1106 36-37 0.5611378360691717 1106 38-39 0.5406597604124457 1106 40-41 0.4243376399920251 1106 42-43 0.33549842114443607 1106 44-45 0.4958722454335174 1106 46-47 0.4838754079568517 1106 48-49 0.5906703976524099 1106 50-51 0.5538602115431388 1106 52-53 0.5662468034657664 1106 54-55 0.5697286868207314 1106 56-57 0.6026096725123651 1106 58-59 0.8020292104433864 1106 60-61 0.8374657404310071 1106 62-63 0.8852837468586117 1106 64-65 0.8817649044191072 1106 66-67 0.9544582138496409 1106 68-69 0.8936731975854286 1106 70-71 0.6637779487401119 1106 72-73 0.7009933028815638 1106 74-75 0.9205774335443024 1106 76-77 0.658747450321485 1106 78-79 0.6340119003926574 1106 80-81 0.731823744803684 1106 82-83 0.7646220972154154 1106 84-85 0.6477820670132957 1106 86-87 0.5512604695342986 1106 88-89 0.5655969659289717 1106 90-91 0.635410816900464 1106 92-93 0.7032932081350047 1106 94-95 0.6154902467716461 1106 96-97 0.5806568724628676 1106 98-99 0.5505993186986089 1106 100-101 0.5476944474352123 1107 1 0.372022784655627 1107 2 0.38236353137818924 1107 3 0.3672368877112646 1107 4 0.17552720237338093 1107 5 0.39900280019473655 1107 6 0.2103752331875839 1107 7 0.39354820837453985 1107 8 0.3913950326322251 1107 9 0.48199407168007014 1107 10-11 0.45372653885538483 1107 12-13 0.5094794150986388 1107 14-15 0.5369616357240901 1107 16-17 0.4316121930923771 1107 18-19 0.5020501561083535 1107 20-21 0.5322259758265133 1107 22-23 0.6033540847560985 1107 24-25 0.5448485688635358 1107 26-27 0.6312842546362063 1107 28-29 0.5723780647014678 1107 30-31 0.6887898718950325 1107 32-33 0.6095798924356473 1107 34-35 0.569006511586764 1107 36-37 0.5566736669006573 1107 38-39 0.6886314496320196 1107 40-41 0.7969101211012415 1107 42-43 0.6652813041449122 1107 44-45 0.6045873827088926 1107 46-47 0.7084761998465083 1107 48-49 0.5816485119107782 1107 50-51 0.5213673545038802 1107 52-53 0.5341397388237823 1107 54-55 0.5870886195246143 1107 56-57 0.5582827062039328 1107 58-59 0.7149822626994009 1107 60-61 0.5349218311647377 1107 62-63 0.6056662034569698 1107 64-65 0.6918934051950387 1107 66-67 0.7411309719752168 1107 68-69 0.7346658153173777 1107 70-71 0.583028409225399 1107 72-73 0.7275977496465629 1107 74-75 0.6688246887619229 1107 76-77 0.6258013597327832 1107 78-79 0.6567693007900459 1107 80-81 0.6301029076631792 1107 82-83 0.48061870340259105 1107 84-85 0.575269775319434 1107 86-87 0.5009762014520973 1107 88-89 0.621719607301582 1107 90-91 0.6799176262813127 1107 92-93 0.6028682563590557 1107 94-95 0.6275622130075611 1107 96-97 0.6184304442333115 1107 98-99 0.5603369508615152 1107 100-101 0.7768594824610986 1108 1 0.16278160459480517 1108 2 0.163710062970587 1108 3 0.07964303801470862 1108 4 0.0899903834416591 1108 5 0.15677663342012949 1108 6 0.16333777859657772 1108 7 0.10465105724754409 1108 8 0.1857759111480064 1108 9 0.2638896423208692 1108 10-11 0.2615075682236494 1108 12-13 0.3073297673098523 1108 14-15 0.23095498862026176 1108 16-17 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2316 66-67 -0.8000870134242248 2316 68-69 -0.7132842676272446 2316 70-71 -0.9632934159030668 2316 72-73 -0.8500219420422113 2316 74-75 -0.6867765296858792 2316 76-77 -0.7827023424295518 2316 78-79 -0.6147948555735638 2316 80-81 -0.5670957605987965 2316 82-83 -0.5463238480548611 2316 84-85 -0.5095201464756371 2316 86-87 -0.5082127330996826 2316 88-89 -0.5511404503839437 2316 90-91 -0.27370563751726706 2316 92-93 -0.24130856885611252 2316 94-95 -0.3941002052760929 2316 96-97 -0.5509109696979202 2316 98-99 -0.3072608920003361 2316 100-101 -0.3727269504577997 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 45.0 7 1001.0 8 1768.0 9 1292.0 10 1302.0 11 1497.0 12 1777.0 13 1803.0 14 1764.0 15 1871.0 16 2418.0 17 3223.0 18 4352.0 19 5753.0 20 8074.0 21 10513.0 22 11008.0 23 9990.0 24 8180.0 25 6819.0 26 6319.0 27 6419.0 28 6879.0 29 7841.0 30 8946.0 31 10982.0 32 13629.0 33 18006.0 34 25477.0 35 39860.0 36 70679.0 37 159352.0 38 302333.0 39 123665.0 40 155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.77970874312988 21.282113284639863 14.546911158518947 21.39126681371131 2 14.445441314723748 22.889811433323693 42.07134075788256 20.593406494070003 3 16.545121311831068 29.827162279433033 33.06685258171825 20.560863827017645 4 10.910381110789702 19.713963516054385 42.29767048741683 27.07798488573908 5 10.935353087937518 40.21945961093434 36.47976478883425 12.365422512293897 6 25.327912568701187 41.14884654324559 20.584140873589817 12.939100014463408 7 22.96823022129014 36.49795704367949 24.785986765982067 15.747825969048307 8 23.79083652733584 35.71433414087359 23.05207278709864 17.442756544691928 9 26.45933522562916 16.091557889788835 22.404609307202776 35.04449757737923 10-11 21.90946358837142 28.2147183251374 31.41536872649696 18.460449359994215 12-13 23.861910616141163 27.126403402516637 30.321969012149264 18.689716969192943 14-15 21.422962015475846 27.96663698654903 29.208060637836276 21.40234036013885 16-17 19.242320834538617 30.928697660543826 29.614053008388773 20.214928496528785 18-19 18.929323654903097 29.383542450101245 32.88928035507666 18.797853539919004 20-21 20.888606902661266 28.43726270971941 32.51136733439398 18.16276305322534 22-23 20.329675296499854 28.747604498119756 31.84373418064796 19.078986024732426 24-25 19.570120407868092 28.953538563060455 32.33034874891524 19.145992280156204 26-27 19.41390430647961 29.088059553080708 32.409106522996815 19.088929617442872 28-29 19.388367352473242 29.09670369901649 32.52955958923923 18.985369359271047 30-31 19.999954801851317 28.887097284495226 32.1669574414232 18.945990472230257 32-33 19.294524696268443 29.27630984234886 32.15791781168643 19.271247649696267 34-35 19.664076059444604 29.361451854932024 31.848536483945615 19.125935601677753 36-37 19.820461653890657 29.123427104425804 31.64215043751808 19.413960804165463 38-39 19.385316477437083 29.287948365634943 31.98949821015331 19.33723694677466 40-41 19.536391289412787 29.12551751880243 32.06712603051779 19.270965161266997 42-43 19.525600231414522 29.14500922042233 31.62395818267284 19.70543236549031 44-45 19.904078228955743 29.173484054093144 31.353560258171825 19.568877458779287 46-47 19.60205267508219 29.229037793018353 31.080637497648283 20.088272034251176 48-49 19.59131695422203 29.2235270720879 31.107505291026214 20.077650682663855 50-51 19.622493762655484 29.61021116575065 30.290499801128146 20.476795270465722 52-53 19.5583688892103 29.76032551706682 30.347392970783915 20.333912622938964 54-55 19.78639354932022 29.41173479534278 30.27202505785363 20.529846597483367 56-57 19.74594120624819 29.725805431009544 29.901343740960368 20.62690962178189 58-59 19.626618093722882 29.416989080127276 30.049311180214062 20.907081645935783 60-61 19.97413535941568 29.36693213045994 29.969592945472957 20.689339564651434 62-63 19.632324359994215 29.17495299392537 30.314567815302283 20.878154830778133 64-65 19.57028990092566 28.83161655698583 30.361065410760773 21.23702813132774 66-67 19.59780427393694 29.16020709791727 29.939818665027477 21.30216996311831 68-69 19.811704512583166 29.175065989297078 29.86462024515476 21.148609252965 70-71 19.69221190700029 28.750429382412495 30.190781385594445 21.366577324992768 72-73 19.75396387763957 28.541274949378074 30.132701764535724 21.57205940844663 74-75 19.79831456103558 28.60602129736766 29.86467674284061 21.730987398756145 76-77 19.720517247613536 28.7003159350593 29.96529912134799 21.613867695979174 78-79 19.898089474255134 28.7510508569569 29.729929762076946 21.62092990671102 80-81 19.920519055539483 28.70494874529939 29.678064886462252 21.69646731269887 82-83 20.07826059444605 28.662801471651722 29.599137619323113 21.659800314579115 84-85 20.15577541943882 28.30991692580272 29.529758461093436 22.004549193665028 86-87 20.03362742262077 28.366075625542376 29.712358981776106 21.887937970060747 88-89 20.449111404396874 28.06669438819786 29.455351008822678 22.02884319858259 90-91 20.687362145646514 27.98352979461961 29.523261227220132 21.80584683251374 92-93 20.807306732716228 27.879913038761934 29.47162234234886 21.841157886172983 94-95 20.749114116285796 27.587820002892684 29.454447045849 22.20861883497252 96-97 20.89742054165461 27.347704838009832 29.379587612091406 22.375287008244143 98-99 20.97301444532832 27.237534350593002 29.38845774877061 22.400993455308072 100-101 21.44945943014174 27.042108855221294 29.400774244286954 22.107657470350013 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 328.0 1 210.0 2 112.5 3 124.5 4 116.0 5 138.5 6 192.0 7 319.0 8 532.0 9 731.0 10 746.5 11 731.0 12 824.0 13 933.5 14 1042.0 15 1159.0 16 1334.0 17 1511.0 18 1732.0 19 1968.0 20 2175.0 21 2502.0 22 2913.0 23 3435.0 24 4244.5 25 5386.0 26 7206.5 27 9647.0 28 12201.0 29 15529.0 30 19681.0 31 23393.5 32 26823.5 33 29915.5 34 32947.0 35 34859.0 36 36442.0 37 38152.5 38 37151.5 39 35120.0 40 33497.5 41 32936.0 42 32712.0 43 32087.5 44 31813.0 45 31769.5 46 31311.0 47 30565.0 48 29340.5 49 27344.0 50 25657.0 51 24027.5 52 22118.0 53 19590.0 54 16976.0 55 14356.5 56 11872.5 57 9588.5 58 7597.0 59 6300.5 60 4969.5 61 3548.5 62 2567.5 63 1987.5 64 1449.5 65 1028.0 66 776.5 67 602.0 68 443.5 69 336.0 70 279.5 71 228.5 72 176.0 73 131.5 74 115.0 75 102.5 76 74.0 77 59.5 78 59.0 79 48.0 80 35.0 81 31.0 82 25.0 83 23.0 84 20.5 85 15.0 86 9.5 87 8.0 88 10.0 89 7.0 90 7.0 91 6.5 92 2.5 93 2.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 5.649768585478738E-5 48-49 3.3898611512872437E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 884992.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.58445756462772 #Duplication Level Percentage of deduplicated Percentage of total 1 66.45110323237643 29.626863921865827 2 14.504933578393949 12.933891912072976 3 6.655142565320501 8.901477638702387 4 3.73911964943736 6.668264853576226 5 2.3260874619151837 5.185367386868505 6 1.5274385726188469 4.086001213410031 7 1.0428554311753158 3.254660060209419 8 0.7331823751830805 2.6150830794786413 9 0.5462873024612941 2.1920330749212464 >10 2.344297508143013 17.05328801939774 >50 0.08482682383007834 2.6088475787442995 >100 0.04369732675283354 3.3868113649304794 >500 2.5704309802665703E-4 0.06658333842921212 >1k 5.140861960533141E-4 0.7854771111769705 >5k 2.5704309802665703E-4 0.6353494462160963 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5544 0.6264463407578825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4415 0.4988745660977727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2439 0.27559571159965285 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.1299537170957478E-4 0.0 3 0.0 0.0 0.0 1.1299537170957478E-4 0.0 4 0.0 0.0 0.0 1.1299537170957478E-4 0.0 5 0.0 0.0 0.0 1.1299537170957478E-4 0.0 6 0.0 0.0 0.0 1.1299537170957478E-4 0.0 7 0.0 0.0 0.0 2.2599074341914956E-4 0.0 8 0.0 0.0 0.0 2.2599074341914956E-4 0.0 9 0.0 0.0 0.0 3.389861151287243E-4 0.0 10-11 0.0 0.0 0.0 3.389861151287243E-4 0.0 12-13 0.0 0.0 0.0 4.519814868382991E-4 0.0 14-15 0.0 0.0 0.0 5.649768585478739E-4 0.0 16-17 0.0 0.0 0.0 0.0010734560312409603 0.0 18-19 0.0 0.0 0.0 0.0014689398322244721 0.0 20-21 0.0 0.0 0.0 0.002316405120046283 0.0 22-23 0.0 0.0 0.0 0.004180828753254267 0.0 24-25 0.0 0.0 0.0 0.00887013667920162 0.0 26-27 0.0 0.0 0.0 0.01683631038472664 0.0 28-29 0.0 0.0 0.0 0.03779695183685276 0.0 30-31 0.0 0.0 0.0 0.09474661917847844 0.0 32-33 0.0 0.0 0.0 0.19367406711021118 0.0 34-35 0.0 0.0 0.0 0.3476302610645068 0.0 36-37 0.0 0.0 0.0 0.5979150094012149 0.0 38-39 0.0 0.0 0.0 0.9200648141452126 0.0 40-41 0.0 0.0 0.0 1.2549830958923922 0.0 42-43 0.0 0.0 0.0 1.561991520827307 0.0 44-45 0.0 0.0 0.0 1.9141980944460515 0.0 46-47 0.0 0.0 0.0 2.3576484307202774 0.0 48-49 0.0 0.0 0.0 2.809686415244432 0.0 50-51 0.0 0.0 0.0 3.2542102075498986 0.0 52-53 0.0 0.0 0.0 3.669468198582586 0.0 54-55 0.0 0.0 0.0 4.101957983800983 0.0 56-57 0.0 0.0 0.0 4.626708490020249 0.0 58-59 0.0 0.0 0.0 5.19637465649407 0.0 60-61 0.0 0.0 0.0 5.756662207115997 0.0 62-63 0.0 0.0 0.0 6.299039991321955 0.0 64-65 0.0 0.0 0.0 6.846728557998264 0.0 66-67 0.0 0.0 0.0 7.4600674356378365 0.0 68-69 0.0 0.0 0.0 8.139904089528493 0.0 70-71 0.0 0.0 0.0 8.848554563205091 0.0 72-73 0.0 0.0 0.0 9.540142735753545 0.0 74-75 0.0 5.649768585478739E-5 0.0 10.215120588660689 0.0 76-77 0.0 1.1299537170957478E-4 0.0 10.931059263812553 0.0 78-79 0.0 1.1299537170957478E-4 0.0 11.714230185131617 0.0 80-81 0.0 1.1299537170957478E-4 0.0 12.536610500433902 0.0 82-83 0.0 1.1299537170957478E-4 0.0 13.367917450101244 0.0 84-85 0.0 1.1299537170957478E-4 0.0 14.198828915967603 0.0 86-87 0.0 1.1299537170957478E-4 0.0 15.034712178189181 0.0 88-89 0.0 1.1299537170957478E-4 0.0 15.931612941133931 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2835 0.0 55.291 1 GTATCAA 5015 0.0 41.864407 1 AACGCAG 5835 0.0 35.736935 6 TCAACGC 6030 0.0 34.81758 4 ATCAACG 6005 0.0 34.80433 3 CAACGCA 6025 0.0 34.688797 5 TATCAAC 6090 0.0 34.552544 2 ACGCAGA 6595 0.0 31.61865 7 CGCAGAG 6605 0.0 31.498865 8 GCAGAGT 7240 0.0 28.145718 9 GTACATG 5015 0.0 28.035892 1 TACATGG 5135 0.0 27.288218 2 ACATGGG 4990 0.0 26.462929 3 GAGTACT 4225 0.0 25.970413 12-13 CATGGGG 2005 0.0 25.586035 4 CAGAGTA 7110 0.0 23.883615 10-11 GTACTTT 4645 0.0 23.366524 14-15 ACGATAC 150 3.0321962E-5 22.166666 3 AGAGTAC 6720 0.0 22.053572 10-11 CGATACT 110 0.0028768487 21.59091 4 >>END_MODULE