FastQCFastQC Report
Fri 10 Feb 2017
SRR2031526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884992
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT67450.7621537821810819No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66150.7474643838588371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52450.5926607246167197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30560.3453138559444605No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT20070.22678171102111658RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT13620.15389969626844086RNA PCR Primer, Index 39 (96% over 25bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG12960.14644200173560892RNA PCR Primer, Index 39 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.055.7517281
GTATCAA47550.047.2029231
CGTCGCG308.293384E-539.58267656-57
ATCAACG58550.038.2102973
AACGCAG59250.037.598536
TCAACGC59350.037.535184
CAACGCA59800.037.2527285
TATCAAC60250.037.2112
CGCGCTC352.0539797E-433.92800558-59
GCGTCGC352.0539797E-433.92800554-55
CGCAGAG66850.033.3240558
ACGCAGA67150.033.3166477
GCAGAGT73800.029.7352779
TCGCGCT404.4950482E-429.68700858-59
TGCGTCG404.4950482E-429.68700854-55
TAACGTT650.005843831729.2302845
GTACATG48650.028.150561
TACATGG50150.027.3724272
ACATGGG47550.027.3707313
CATGGGG17800.026.1512494
Produced by FastQC (version 0.11.5)