##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031526_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 884992 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74153890656639 34.0 31.0 34.0 31.0 34.0 2 32.87987462033555 34.0 33.0 34.0 31.0 34.0 3 32.973625750289266 34.0 33.0 34.0 31.0 34.0 4 36.36150270284929 37.0 37.0 37.0 35.0 37.0 5 36.30391574161122 37.0 37.0 37.0 35.0 37.0 6 36.323211961238066 37.0 37.0 37.0 35.0 37.0 7 36.34579634618166 37.0 37.0 37.0 35.0 37.0 8 36.37888930069424 37.0 37.0 37.0 35.0 37.0 9 38.23021564036737 39.0 39.0 39.0 37.0 39.0 10-11 38.19831478702632 39.0 39.0 39.0 37.0 39.0 12-13 38.20587022255568 39.0 39.0 39.0 37.0 39.0 14-15 39.72013532325716 41.0 40.0 41.0 37.0 41.0 16-17 39.70995048542812 41.0 40.0 41.0 37.0 41.0 18-19 39.765058893187735 41.0 40.0 41.0 37.5 41.0 20-21 39.77028888396731 41.0 40.0 41.0 37.0 41.0 22-23 39.73942928297657 41.0 40.0 41.0 37.0 41.0 24-25 39.684853648394565 41.0 40.0 41.0 37.0 41.0 26-27 39.56764241936651 41.0 40.0 41.0 36.5 41.0 28-29 39.4664234252965 41.0 39.5 41.0 36.0 41.0 30-31 39.291581731812265 41.0 39.0 41.0 35.5 41.0 32-33 38.347315003977435 40.0 38.0 41.0 34.5 41.0 34-35 38.50310906765259 40.0 38.0 41.0 35.0 41.0 36-37 38.682042888523284 40.0 38.0 41.0 35.0 41.0 38-39 38.587926218542094 40.0 38.0 41.0 35.0 41.0 40-41 38.47922015114261 40.0 38.0 41.0 35.0 41.0 42-43 38.248858181768874 40.0 38.0 41.0 34.5 41.0 44-45 38.023343713841484 40.0 38.0 41.0 33.5 41.0 46-47 37.68871244033845 40.0 38.0 41.0 33.0 41.0 48-49 37.42849935366647 40.0 38.0 41.0 32.0 41.0 50-51 37.37022990038328 40.0 38.0 41.0 31.5 41.0 52-53 37.46765676977871 40.5 38.0 41.0 32.0 41.0 54-55 37.37238811198293 40.5 38.0 41.0 31.5 41.0 56-57 37.215901951656065 40.0 38.0 41.0 31.0 41.0 58-59 37.070570129447496 40.0 37.0 41.0 31.0 41.0 60-61 36.83560416365346 40.0 37.0 41.0 30.0 41.0 62-63 36.54433881888198 40.0 36.5 41.0 28.5 41.0 64-65 36.302753019236334 39.5 36.0 41.0 28.0 41.0 66-67 35.99166885124386 39.0 35.0 41.0 28.0 41.0 68-69 35.589478209972526 39.0 35.0 41.0 26.0 41.0 70-71 35.16313763288256 38.0 35.0 40.5 25.5 41.0 72-73 34.76494250795487 37.0 35.0 40.0 25.0 41.0 74-75 34.333916577776975 37.0 35.0 39.0 24.0 41.0 76-77 32.842265240815735 35.5 33.0 38.0 21.0 39.0 78-79 33.49586945418716 36.0 34.0 37.5 23.0 39.5 80-81 33.306042879483655 35.0 34.0 37.0 23.0 39.0 82-83 33.00664017301851 35.0 34.0 37.0 23.0 39.0 84-85 32.67963269724472 35.0 34.0 36.0 21.5 38.0 86-87 32.421976130857686 35.0 34.0 36.0 20.5 37.0 88-89 32.189282501988714 35.0 34.0 36.0 20.0 37.0 90-91 31.996903926815158 35.0 34.0 35.0 20.0 36.5 92-93 31.83717423434336 35.0 34.0 35.0 19.0 36.0 94-95 31.71786242135522 35.0 34.0 35.0 19.0 36.0 96-97 31.617456993961525 35.0 34.0 35.0 19.0 36.0 98-99 31.546734885739077 35.0 34.0 35.0 19.0 35.5 100-101 30.47306077343072 34.5 31.5 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38562287192188904 1101 2 0.4209845518580977 1101 3 0.33502754946957936 1101 4 0.18376848371687515 1101 5 0.20424843267684878 1101 6 0.3240384137271093 1101 7 0.27085027843368437 1101 8 0.2518028027051784 1101 9 0.3123560108907881 1101 10-11 0.31168438799429765 1101 12-13 0.266996992921527 1101 14-15 0.21792630279049519 1101 16-17 0.2544861883095919 1101 18-19 0.37706587571967276 1101 20-21 0.3542445736390718 1101 22-23 0.2991681634911245 1101 24-25 0.350972117970926 1101 26-27 0.343636042569166 1101 28-29 0.4424821842377469 1101 30-31 0.345353212525481 1101 32-33 0.2886617927361854 1101 34-35 -0.06365885612391509 1101 36-37 0.3085499134046614 1101 38-39 0.31956727191607115 1101 40-41 0.4596793481037267 1101 42-43 0.6009678920286134 1101 44-45 0.4678610983972007 1101 46-47 0.4999383587135071 1101 48-49 0.4199021778302878 1101 50-51 0.36712378967285275 1101 52-53 0.3400046250749327 1101 54-55 0.4118511570673675 1101 56-57 0.31076655338072356 1101 58-59 0.28256109736097557 1101 60-61 0.4346112924054992 1101 62-63 0.552122627163115 1101 64-65 0.65987589871051 1101 66-67 0.4878890193313765 1101 68-69 0.3100115741082661 1101 70-71 0.25099165609063334 1101 72-73 0.3985423610715131 1101 74-75 0.32557486299555194 1101 76-77 0.3995699686286329 1101 78-79 0.40146131501328597 1101 80-81 0.5615177889207885 1101 82-83 0.36834108752155714 1101 84-85 0.34677822396631797 1101 86-87 0.25438738460543675 1101 88-89 0.1475544713797916 1101 90-91 0.21118586561175334 1101 92-93 0.1919879527865973 1101 94-95 0.03576933250113612 1101 96-97 0.20342067216923354 1101 98-99 0.27883596657927257 1101 100-101 -0.0030689300385695617 1102 1 0.15604088323975418 1102 2 0.216981755321342 1102 3 0.1267666204317024 1102 4 0.07270240540371731 1102 5 0.049387676902661326 1102 6 0.15069113148461355 1102 7 0.037871409511211596 1102 8 0.07007097639443316 1102 9 0.0725808483927537 1102 10-11 0.10904059686316003 1102 12-13 0.1520641452669267 1102 14-15 0.1662790311869955 1102 16-17 0.2170179153230194 1102 18-19 0.2177000106931004 1102 20-21 0.3169308194404721 1102 22-23 0.1780545643179252 1102 24-25 0.17135922553119798 1102 26-27 0.12520727066422666 1102 28-29 0.20488674988796873 1102 30-31 0.1520464091392455 1102 32-33 0.29746049781807926 1102 34-35 -0.098143143336344 1102 36-37 -0.07391955013650175 1102 38-39 0.16888738924902214 1102 40-41 0.24205230072978878 1102 42-43 0.18840019506782113 1102 44-45 -0.00791111557838775 1102 46-47 0.05294336917518905 1102 48-49 0.03162686081346777 1102 50-51 0.08072194963273205 1102 52-53 0.1250195956657052 1102 54-55 0.1108130947836301 1102 56-57 0.12788850163465781 1102 58-59 0.2070710701555356 1102 60-61 0.1833510777003724 1102 62-63 0.3808993449814082 1102 64-65 0.17031984892000196 1102 66-67 0.26547442262541665 1102 68-69 0.2908993732034446 1102 70-71 0.06696864545669712 1102 72-73 -0.003538323066756277 1102 74-75 0.1449791297405767 1102 76-77 -0.14278717748534575 1102 78-79 0.10276889859274974 1102 80-81 -0.008243644202977407 1102 82-83 0.23242534838980333 1102 84-85 0.0817747029644309 1102 86-87 0.14340489842347637 1102 88-89 -0.01824545465384375 1102 90-91 -0.09578723798676236 1102 92-93 -0.22554967633791279 1102 94-95 0.0027973029348515865 1102 96-97 0.09918128457025333 1102 98-99 0.07410363871573367 1102 100-101 0.07306323672013093 1103 1 0.3255147996841288 1103 2 0.2981045215220277 1103 3 0.2897983699653892 1103 4 0.13828334364956874 1103 5 0.23475039978117707 1103 6 0.23970414819172703 1103 7 0.032873314884362514 1103 8 0.15917825893118476 1103 9 0.10616555727178678 1103 10-11 0.16695290958504216 1103 12-13 0.17049512061741012 1103 14-15 0.1685353941304939 1103 16-17 0.21800469805030787 1103 18-19 0.2482892724111494 1103 20-21 0.16028808693480556 1103 22-23 0.1831729983017567 1103 24-25 0.08742753817284665 1103 26-27 0.19557470683422906 1103 28-29 0.1867064815311963 1103 30-31 0.30114787527374176 1103 32-33 0.5545384576648473 1103 34-35 0.3606265873040613 1103 36-37 0.21190347078248806 1103 38-39 0.1295805439452664 1103 40-41 0.019083528792926074 1103 42-43 0.017280637916655905 1103 44-45 0.12395675180764698 1103 46-47 -0.031499760450351744 1103 48-49 0.07482467865979459 1103 50-51 0.08702472345490975 1103 52-53 0.262941535536136 1103 54-55 0.2080733687562386 1103 56-57 0.14206247223174273 1103 58-59 0.3870806973040999 1103 60-61 0.28548108431903785 1103 62-63 0.2615457678940203 1103 64-65 0.14927249538302334 1103 66-67 0.21272667397421685 1103 68-69 0.3376482049088523 1103 70-71 0.38504863101597664 1103 72-73 0.18825653844270107 1103 74-75 0.15707223228976375 1103 76-77 0.2582607777484114 1103 78-79 0.2453941016654113 1103 80-81 0.09423334089500202 1103 82-83 0.18959055427038862 1103 84-85 0.3196013741772532 1103 86-87 0.2979978505484695 1103 88-89 0.3078782140920211 1103 90-91 0.3042227902929895 1103 92-93 0.2987709077090983 1103 94-95 0.14092883385203692 1103 96-97 0.2669534019412403 1103 98-99 0.2602124656075695 1103 100-101 0.35157865018610224 1104 1 0.22321480511291014 1104 2 0.20760127329995726 1104 3 0.22660009269932146 1104 4 0.2216296457432705 1104 5 0.16055702651613046 1104 6 0.2911527285648674 1104 7 0.23026026046986203 1104 8 0.17500859135368785 1104 9 0.21342926947388463 1104 10-11 0.16088081534136478 1104 12-13 0.12868353559361623 1104 14-15 0.14744457058404947 1104 16-17 0.2060649937380461 1104 18-19 0.1928098203563522 1104 20-21 0.22286295890599916 1104 22-23 0.21507072002231808 1104 24-25 0.19148374417707714 1104 26-27 0.12753304996952863 1104 28-29 0.11851281873103403 1104 30-31 0.18578713261617708 1104 32-33 0.4706483724329473 1104 34-35 0.3899745904165641 1104 36-37 0.15896107017447036 1104 38-39 0.2559933848276259 1104 40-41 0.34175231871710565 1104 42-43 0.1883895574077954 1104 44-45 0.10170416861154052 1104 46-47 -0.0457276969697773 1104 48-49 0.11624267688639378 1104 50-51 0.32937918681967204 1104 52-53 0.2576692820481483 1104 54-55 0.2607470445854787 1104 56-57 0.18770406395104544 1104 58-59 0.19012624479690032 1104 60-61 0.4108606467590974 1104 62-63 0.4004620017660159 1104 64-65 0.336485900858861 1104 66-67 0.35665367493331246 1104 68-69 0.4587616483861723 1104 70-71 0.4783540362746592 1104 72-73 0.35687817033499414 1104 74-75 0.3632474734182267 1104 76-77 0.15721030815864623 1104 78-79 0.20942207799592438 1104 80-81 0.26370910137707426 1104 82-83 0.18756923724753705 1104 84-85 0.20528567232603478 1104 86-87 0.11072020446895436 1104 88-89 0.2812626986290354 1104 90-91 0.2616719859282455 1104 92-93 0.364877203288259 1104 94-95 0.3385112156402812 1104 96-97 0.2139132832318431 1104 98-99 0.37601203492408786 1104 100-101 0.4030163729924219 1105 1 0.27257154103841685 1105 2 0.2283618575455293 1105 3 0.1544261707512007 1105 4 0.10771097452989409 1105 5 0.09429519465569314 1105 6 0.10077455277917124 1105 7 0.10362479270816038 1105 8 0.09009397872871716 1105 9 0.1191208508897148 1105 10-11 0.1924458124911368 1105 12-13 0.12440648658675713 1105 14-15 0.13147475910871975 1105 16-17 0.12442216999820488 1105 18-19 0.2729131795294748 1105 20-21 0.3100509271882501 1105 22-23 0.1351994752406327 1105 24-25 0.04942888325797412 1105 26-27 0.1451233953762241 1105 28-29 0.21274272803574235 1105 30-31 0.11673139444121006 1105 32-33 0.5534995563870169 1105 34-35 0.4437935548450369 1105 36-37 0.20020830954926083 1105 38-39 0.23669887603971063 1105 40-41 -0.01412768496941652 1105 42-43 0.13874182513126243 1105 44-45 0.06013777194852565 1105 46-47 -0.08523237168535758 1105 48-49 -0.10329998949354291 1105 50-51 0.14161571138453866 1105 52-53 0.10973987895761184 1105 54-55 -0.060375535707329675 1105 56-57 0.11982255152097565 1105 58-59 0.07737838588148804 1105 60-61 0.007901529613015157 1105 62-63 0.13029023711635546 1105 64-65 0.18866402134671034 1105 66-67 0.26394786968410244 1105 68-69 0.16806482732929595 1105 70-71 0.28809277104641495 1105 72-73 0.35087733062704984 1105 74-75 0.503721908407428 1105 76-77 0.43048830951720163 1105 78-79 0.48016986181549726 1105 80-81 0.489242367124163 1105 82-83 0.5058731637355365 1105 84-85 0.45001445689998576 1105 86-87 0.2608965090030324 1105 88-89 0.3986519920113949 1105 90-91 0.4968832270916863 1105 92-93 0.36860383397718977 1105 94-95 0.3689685492603445 1105 96-97 0.3048440960759571 1105 98-99 0.41601933970051874 1105 100-101 0.45653068728213597 1106 1 0.22651488372480344 1106 2 0.17252253984289467 1106 3 0.20184139879133767 1106 4 0.16800012657250107 1106 5 0.04488570526903146 1106 6 0.15088155805810288 1106 7 0.07544832118741596 1106 8 0.10976234162720999 1106 9 0.11370400588091911 1106 10-11 0.10209451812357173 1106 12-13 0.1255795814964742 1106 14-15 0.1260864488409723 1106 16-17 0.17693401984205792 1106 18-19 0.14127607802506503 1106 20-21 0.1599645303334114 1106 22-23 0.20416635908837577 1106 24-25 0.23355326302367985 1106 26-27 0.17016500854024486 1106 28-29 0.17980254002350904 1106 30-31 0.13912165458292947 1106 32-33 0.5760150523366647 1106 34-35 0.3266069217859453 1106 36-37 0.15009575584846147 1106 38-39 0.1824337248848451 1106 40-41 0.21499915195453667 1106 42-43 0.3723819069308547 1106 44-45 0.16904457431373743 1106 46-47 0.22298801163133675 1106 48-49 0.28634665529648373 1106 50-51 0.13236887002184972 1106 52-53 0.27222382816577095 1106 54-55 0.5120400886486607 1106 56-57 0.37847729700693833 1106 58-59 0.48882874335838267 1106 60-61 0.3440154149350505 1106 62-63 0.29497294664854223 1106 64-65 0.5828215331172899 1106 66-67 0.48261910190933577 1106 68-69 0.423903352400238 1106 70-71 0.4318214690695896 1106 72-73 0.32765480720478024 1106 74-75 0.3787421205156676 1106 76-77 0.4558025879845644 1106 78-79 0.37819937941984705 1106 80-81 0.28039184160934383 1106 82-83 0.3775223132417267 1106 84-85 0.5292744559488298 1106 86-87 0.45844410653636203 1106 88-89 0.3772453878040949 1106 90-91 0.4860027718040385 1106 92-93 0.47244096401635716 1106 94-95 0.5668974026393272 1106 96-97 0.5410562677282833 1106 98-99 0.3898704200575196 1106 100-101 0.3288456474127628 1107 1 0.2918414294325018 1107 2 0.27602802940764803 1107 3 0.22515573257427945 1107 4 0.1610399096005395 1107 5 0.14591980293542406 1107 6 0.15380110729014262 1107 7 0.14227452606348123 1107 8 0.20230465551138366 1107 9 0.20532679953999633 1107 10-11 0.2833524043873368 1107 12-13 0.17592113829932288 1107 14-15 0.24683294262623434 1107 16-17 0.2542121529549348 1107 18-19 0.21526844919795707 1107 20-21 0.3313687566456096 1107 22-23 0.3237474987713327 1107 24-25 0.2808599679795094 1107 26-27 0.2607014920280406 1107 28-29 0.29012466217176325 1107 30-31 0.3337169351526228 1107 32-33 0.8630685266437652 1107 34-35 0.6988240677924225 1107 36-37 0.38850893080386584 1107 38-39 0.13402693145781797 1107 40-41 0.13944040563479376 1107 42-43 0.2752666478262995 1107 44-45 0.29615954334882133 1107 46-47 0.3654933416578743 1107 48-49 0.38401377157083516 1107 50-51 0.5196326446958821 1107 52-53 0.4533816404618207 1107 54-55 0.25265706641483376 1107 56-57 0.48397121279977284 1107 58-59 0.3546201253943835 1107 60-61 0.28381004574913504 1107 62-63 0.3982681358463509 1107 64-65 0.4314756107688851 1107 66-67 0.39419142348035763 1107 68-69 0.3580147970912435 1107 70-71 0.5688256315665114 1107 72-73 0.4848594077066011 1107 74-75 0.6413188076322314 1107 76-77 0.445002576381782 1107 78-79 0.346380208728462 1107 80-81 0.33460219999017937 1107 82-83 0.41227263594401364 1107 84-85 0.20298328421319667 1107 86-87 0.3672245094428632 1107 88-89 0.39932547598522206 1107 90-91 0.2767089459327394 1107 92-93 0.4163799624064666 1107 94-95 0.40770075004103035 1107 96-97 0.46091369858297426 1107 98-99 0.33470645828867873 1107 100-101 0.4219905806242643 1108 1 0.15280395351959442 1108 2 0.12443461681013446 1108 3 0.03546407396996187 1108 4 0.14294427552706424 1108 5 0.07896902188740285 1108 6 0.0790131936118712 1108 7 0.06724807357534957 1108 8 0.030296851694231464 1108 9 0.050930889081335806 1108 10-11 0.10143191377030547 1108 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-0.6364594482931345 2316 100-101 -0.5333450416337904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 0.0 12 2.0 13 15.0 14 86.0 15 300.0 16 886.0 17 1956.0 18 3123.0 19 4611.0 20 6720.0 21 9424.0 22 11898.0 23 10442.0 24 8407.0 25 7200.0 26 6888.0 27 7422.0 28 8796.0 29 10472.0 30 9917.0 31 10397.0 32 12674.0 33 16210.0 34 21964.0 35 33324.0 36 59658.0 37 150688.0 38 336935.0 39 134455.0 40 120.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.88707213578501 21.51988684582744 14.799547383309761 21.793493635077795 2 14.210862923054671 22.738058649117733 42.57722103702633 20.47385739080127 3 16.079580380387622 29.87100448365635 33.902114369395434 20.1473007665606 4 10.497722013306335 20.438941820943015 41.883655445472954 27.179680720277698 5 10.422919077234596 40.169741647382125 37.068922656927974 12.338416618455309 6 24.914236512872435 41.77371094879953 20.1932898828464 13.11876265548163 7 22.24969265258895 36.6054156421753 24.52112561469482 16.623766090540933 8 23.33591716083309 36.0590830199595 23.12676272779867 17.478237091408737 9 25.875488140005785 15.674830958923922 22.27285670378941 36.17682419728088 10-11 21.47019408085045 28.240594265258895 31.585426760919873 18.703784892970784 12-13 23.481059715794043 26.85024271405843 30.598977165895285 19.06972040425224 14-15 21.012280336997396 27.688724869829333 29.33501093795198 21.96398385522129 16-17 19.000680232137693 31.040619576222156 29.701906909892973 20.25679328174718 18-19 18.678021948221 28.850034802574488 33.22335117153602 19.2485920776685 20-21 20.192498915244432 27.89618437228811 33.04611793100955 18.865198781457913 22-23 19.66164665895285 28.623083598495807 32.3835130713046 19.331756671246747 24-25 19.194693285363034 28.878848622360426 33.01380125470061 18.912656837575934 26-27 19.049437735030374 28.89986576149841 33.1240282398033 18.92666826366792 28-29 18.573614224761354 28.382007972953428 33.30578129519815 19.73859650708707 30-31 19.198365634943592 28.274662369829333 33.33544258027191 19.191529414955163 32-33 18.634292739369396 28.761050947353194 33.19436785869251 19.4102884545849 34-35 18.95745950245878 29.22224155698583 33.008207983800986 18.812090956754414 36-37 19.1365006689326 28.782463570292162 32.340744323112524 19.740291437662712 38-39 18.848814452560024 28.65771667992479 32.983292504339026 19.510176363176164 40-41 18.99084963479896 28.427771098495807 33.20357698148684 19.377802285218397 42-43 19.36453626955739 28.387504286764337 32.72093573780087 19.5270237058774 44-45 19.216557889788835 28.777435276251083 32.438711310384726 19.567295523575353 46-47 19.4346026223473 28.398121745301115 31.719192899650327 20.44808273270126 48-49 19.365919665078124 28.53643998610142 31.717632834547576 20.38000751427288 50-51 19.075989037158067 29.005880860733374 31.181273902119745 20.73685619998881 52-53 19.05068068411918 29.309473983945615 31.508250922042237 20.13159440989297 54-55 19.19819614188603 29.08517817110211 30.94304807275094 20.77357761426092 56-57 19.55334059516922 29.52512565085334 29.941174609487998 20.98035914448944 58-59 18.87864523069135 29.52365671102112 30.86479877784206 20.732899280445473 60-61 19.727353467601965 29.939140692797224 29.81298136028348 20.520524479317327 62-63 18.845876572895573 30.259482571593864 29.968406494070006 20.926234361440553 64-65 18.699999548018514 29.793715649406998 30.096938729389645 21.409346073184842 66-67 18.817232246167197 29.94665488501591 29.75252883641886 21.483584032398035 68-69 18.95900131925749 29.921268295163383 29.593911640822967 21.525818744756165 70-71 18.83192164448944 29.29026477075499 29.898575354353486 21.979238230402082 72-73 18.763785435348566 29.005685927104423 29.79880044113393 22.431728196413076 74-75 18.678417432021984 28.865571666184554 29.60834674211744 22.84766415967602 76-77 18.533228850220596 28.85643534429427 29.857913889568433 22.752421915916706 78-79 18.621637257737923 28.914442164448946 29.548628688168932 22.9152918896442 80-81 18.55621293751808 28.768226153456755 29.349022364043968 23.326538544981197 82-83 18.50700345313856 28.600145538038763 29.43580280951692 23.457048199305756 84-85 18.539263631761642 28.275396839745444 29.314841264101823 23.87049826439109 86-87 18.196523013523873 28.383807509798125 29.51206820864179 23.90760126803621 88-89 18.530117323492156 28.07955805100402 29.145051520414377 24.245273105089442 90-91 18.46083006587645 27.85031469282139 29.237336015096215 24.451519226205946 92-93 18.472709357824705 27.653526811541802 29.234388559444607 24.639375271188893 94-95 18.231464239224763 27.371320870697136 29.42631119829332 24.970903691784784 96-97 18.228300368816893 27.251093795198152 29.38455940844663 25.13604642753833 98-99 18.112945090235616 27.22741454143177 29.29162980063528 25.368010567697336 100-101 18.122464660617634 27.164205245257005 29.288636029785646 25.424694064339707 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 322.0 1 205.5 2 103.5 3 133.0 4 129.0 5 133.5 6 178.5 7 320.5 8 600.0 9 769.5 10 744.0 11 756.0 12 870.5 13 979.5 14 1073.0 15 1234.0 16 1430.5 17 1562.0 18 1727.0 19 1965.5 20 2173.5 21 2487.0 22 2863.5 23 3386.0 24 4251.0 25 5317.5 26 6974.5 27 9335.5 28 12009.5 29 15255.0 30 19206.5 31 22865.5 32 26297.5 33 29539.0 34 32522.5 35 34413.0 36 36362.0 37 38257.5 38 37437.0 39 36025.5 40 35072.5 41 34656.5 42 34354.0 43 34061.0 44 33731.5 45 33083.0 46 32265.0 47 31318.5 48 30000.0 49 28147.5 50 26291.5 51 24373.0 52 22231.0 53 19420.0 54 16527.0 55 13740.5 56 10997.0 57 8367.0 58 6388.5 59 5146.5 60 3900.5 61 2675.0 62 1842.5 63 1328.0 64 918.0 65 637.5 66 408.5 67 272.0 68 201.5 69 146.0 70 118.5 71 85.0 72 56.5 73 34.5 74 29.0 75 26.0 76 17.0 77 11.0 78 7.5 79 12.0 80 15.0 81 9.0 82 7.5 83 5.0 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14034025166329187 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 5.649768585478738E-5 44-45 0.0 46-47 0.002429400491755858 48-49 0.0010734560312409605 50-51 2.8248842927393694E-4 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.002768386606884582 70-71 0.0 72-73 0.0 74-75 0.0 76-77 5.649768585478738E-5 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 1.6949305756436218E-4 88-89 3.3898611512872437E-4 90-91 2.2599074341914953E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.003502856522996818 100-101 2.2599074341914953E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 884992.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.51421953201235 #Duplication Level Percentage of deduplicated Percentage of total 1 64.04220146221428 26.586620108157256 2 15.341434056114469 12.737753226828536 3 7.1915361326613745 8.9565302935111 4 3.9824015059492104 6.613051615303683 5 2.4700166903983645 5.127040756646614 6 1.6223998727055444 4.041159869052412 7 1.1606198845431934 3.372756007610147 8 0.8095861559250459 2.688746992572027 9 0.6119808239907167 2.2865315648879183 >10 2.6117485611849163 17.66760822943966 >50 0.10157321362277875 2.893650209568547 >100 0.0517490325759561 3.8190971309609902 >500 8.257824342380971E-4 0.20111938446242078 >1k 0.001101043245650796 0.8822970269513357 >5k 8.257824342380971E-4 2.126037584047352 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 6745 0.7621537821810819 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6615 0.7474643838588371 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5245 0.5926607246167197 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3056 0.3453138559444605 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 2007 0.22678171102111658 RNA PCR Primer, Index 39 (95% over 23bp) ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT 1362 0.15389969626844086 RNA PCR Primer, Index 39 (96% over 25bp) TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG 1296 0.14644200173560892 RNA PCR Primer, Index 39 (96% over 30bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.1299537170957478E-4 0.0 8 0.0 0.0 0.0 1.1299537170957478E-4 0.0 9 0.0 0.0 0.0 2.2599074341914956E-4 0.0 10-11 0.0 0.0 0.0 2.2599074341914956E-4 0.0 12-13 0.0 0.0 0.0 2.8248842927393694E-4 0.0 14-15 0.0 0.0 0.0 3.389861151287243E-4 0.0 16-17 0.0 0.0 0.0 8.474652878218108E-4 0.0 18-19 0.0 0.0 0.0 0.0014689398322244721 0.0 20-21 0.0 0.0 0.0 0.002429400491755858 0.0 22-23 0.0 0.0 0.0 0.004293824124963841 0.0 24-25 0.0 0.0 0.0 0.009265620480185132 0.0 26-27 0.0 0.0 0.0 0.01774027335840324 0.0 28-29 0.0 0.0 0.0 0.03920939398322244 0.0 30-31 0.0 0.0 0.0 0.09683703355510559 0.0 32-33 0.0 0.0 0.0 0.197176923633208 0.0 34-35 0.0 0.0 0.0 0.3517545921319063 0.0 36-37 0.0 0.0 0.0 0.6037907687301128 0.0 38-39 0.0 0.0 0.0 0.9270705271912063 0.0 40-41 0.0 0.0 0.0 1.2650396839745444 0.0 42-43 0.0 0.0 0.0 1.5735170487416836 0.0 44-45 0.0 0.0 0.0 1.9286050043390222 0.0 46-47 0.0 0.0 0.0 2.3741457549898755 0.0 48-49 0.0 0.0 0.0 2.8294606052936073 0.0 50-51 0.0 0.0 0.0 3.2758488212322825 0.0 52-53 0.0 0.0 0.0 3.692914738212323 0.0 54-55 0.0 0.0 0.0 4.1281164123517495 0.0 56-57 0.0 0.0 0.0 4.655409314434481 0.0 58-59 0.0 0.0 0.0 5.226374927682961 0.0 60-61 0.0 0.0 0.0 5.788470404252242 0.0 62-63 0.0 0.0 0.0 6.332430123662135 0.0 64-65 0.0 0.0 0.0 6.883395556118021 0.0 66-67 0.0 0.0 0.0 7.501028257882558 0.0 68-69 0.0 0.0 0.0 8.183859289123518 0.0 70-71 0.0 0.0 0.0 8.895956121637258 0.0 72-73 0.0 0.0 0.0 9.592233602111659 0.0 74-75 0.0 0.0 0.0 10.273878181949668 0.0 76-77 0.0 0.0 0.0 10.992980727509401 0.0 78-79 0.0 0.0 0.0 11.782140403529072 0.0 80-81 0.0 0.0 0.0 12.611526431877351 0.0 82-83 0.0 0.0 0.0 13.448257159386753 0.0 84-85 0.0 0.0 0.0 14.284874891524442 0.0 86-87 0.0 0.0 0.0 15.125616954729534 0.0 88-89 0.0 0.0 0.0 16.026020574197283 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2465 0.0 55.751728 1 GTATCAA 4755 0.0 47.202923 1 CGTCGCG 30 8.293384E-5 39.582676 56-57 ATCAACG 5855 0.0 38.210297 3 AACGCAG 5925 0.0 37.59853 6 TCAACGC 5935 0.0 37.53518 4 CAACGCA 5980 0.0 37.252728 5 TATCAAC 6025 0.0 37.211 2 CGCGCTC 35 2.0539797E-4 33.928005 58-59 GCGTCGC 35 2.0539797E-4 33.928005 54-55 CGCAGAG 6685 0.0 33.324055 8 ACGCAGA 6715 0.0 33.316647 7 GCAGAGT 7380 0.0 29.735277 9 TCGCGCT 40 4.4950482E-4 29.687008 58-59 TGCGTCG 40 4.4950482E-4 29.687008 54-55 TAACGTT 65 0.0058438317 29.230284 5 GTACATG 4865 0.0 28.15056 1 TACATGG 5015 0.0 27.372427 2 ACATGGG 4755 0.0 27.370731 3 CATGGGG 1780 0.0 26.151249 4 >>END_MODULE