FastQCFastQC Report
Fri 10 Feb 2017
SRR2031525_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031525_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286353
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54330.4223568491697069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37820.2940094981704089No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22510.1749908462140641No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.062.0454521
GTATCAA45450.050.687571
TCAACGC58100.039.0791744
ATCAACG58500.038.893163
CAACGCA59150.038.3854645
TATCAAC59750.038.07952
AACGCAG60450.037.6385466
ACGCAGA67300.033.7377
CGCAGAG67700.033.3973438
GCAGAGT75100.029.6005339
GTACATG57400.029.3771781
TACATGG58050.028.2299732
ACATGGG56100.027.6024973
TATGCCG700.00839193527.1428595
CGTATAG1501.0602762E-625.3333341
CATGGGG28500.024.04
AGAGTAC70650.023.43064510-11
GAGTACT47500.023.312-13
CAGAGTA73000.023.29452110-11
CATGGGA29000.022.2758624
Produced by FastQC (version 0.11.5)