##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031525_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1286353 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29431656784724 34.0 31.0 34.0 30.0 34.0 2 32.439354516217556 34.0 31.0 34.0 31.0 34.0 3 32.54002983628911 34.0 31.0 34.0 31.0 34.0 4 35.77275911044636 37.0 35.0 37.0 35.0 37.0 5 35.76988276157478 37.0 35.0 37.0 35.0 37.0 6 35.89736487573784 37.0 37.0 37.0 35.0 37.0 7 35.925750552142375 37.0 37.0 37.0 35.0 37.0 8 35.971760473213806 37.0 37.0 37.0 35.0 37.0 9 37.8014767330585 39.0 39.0 39.0 35.0 39.0 10-11 37.798786569471986 39.0 39.0 39.0 35.0 39.0 12-13 37.77253794253988 39.0 39.0 39.0 35.0 39.0 14-15 39.28379146315203 41.0 40.0 41.0 36.0 41.0 16-17 39.30719367078866 41.0 40.0 41.0 36.5 41.0 18-19 39.28396987452122 41.0 40.0 41.0 36.5 41.0 20-21 39.251781587169305 41.0 40.0 41.0 36.0 41.0 22-23 39.177645249787574 41.0 39.0 41.0 36.0 41.0 24-25 39.12615355194103 41.0 39.0 41.0 36.0 41.0 26-27 39.018622026768696 41.0 39.0 41.0 35.5 41.0 28-29 38.915915771176344 41.0 39.0 41.0 35.0 41.0 30-31 38.72849365609596 40.5 39.0 41.0 35.0 41.0 32-33 38.584836355183995 40.0 38.0 41.0 35.0 41.0 34-35 38.44910611628379 40.0 38.0 41.0 34.5 41.0 36-37 38.30257052302129 40.0 38.0 41.0 34.0 41.0 38-39 38.14691457166112 40.0 38.0 41.0 34.0 41.0 40-41 38.00182181718393 40.0 38.0 41.0 33.0 41.0 42-43 37.832861974901135 40.0 38.0 41.0 33.0 41.0 44-45 37.954727823544545 40.0 38.0 41.0 33.0 41.0 46-47 37.77272101825859 40.0 38.0 41.0 33.0 41.0 48-49 37.59719454924115 40.0 38.0 41.0 32.0 41.0 50-51 36.53158697495944 39.0 36.5 40.0 30.5 40.5 52-53 36.59315638864293 39.5 37.0 40.0 30.5 40.5 54-55 37.35984562557867 40.0 37.0 41.0 32.0 41.0 56-57 37.39681875814804 40.0 37.0 41.0 32.0 41.0 58-59 37.21079283835775 40.0 37.0 41.0 31.0 41.0 60-61 37.04763583557546 40.0 36.0 41.0 31.0 41.0 62-63 36.798961482579045 39.5 36.0 41.0 31.0 41.0 64-65 36.52409058788684 39.0 35.0 41.0 30.0 41.0 66-67 36.23259517410851 39.0 35.0 41.0 30.0 41.0 68-69 35.940023461678095 38.5 35.0 41.0 30.0 41.0 70-71 35.57974871594345 37.0 35.0 40.0 29.0 41.0 72-73 35.21040375386849 37.0 35.0 39.5 29.0 41.0 74-75 34.82712288151075 36.0 35.0 39.0 29.0 41.0 76-77 34.45460849393596 36.0 35.0 39.0 28.0 40.5 78-79 34.050827028039734 35.0 34.0 37.5 27.5 39.5 80-81 33.7025917458116 35.0 34.0 37.0 27.0 39.0 82-83 33.384355227530854 35.0 34.0 37.0 26.0 39.0 84-85 33.066899987794955 35.0 34.0 36.0 26.0 37.5 86-87 32.79981505854147 35.0 34.0 36.0 26.0 37.0 88-89 32.57367612156228 35.0 34.0 35.5 25.0 37.0 90-91 32.37377104107504 35.0 34.0 35.0 25.0 36.0 92-93 32.20404430199176 35.0 34.0 35.0 25.0 36.0 94-95 32.06015961404063 35.0 33.5 35.0 24.0 36.0 96-97 31.92021396926038 35.0 33.0 35.0 24.0 36.0 98-99 31.795563115256854 35.0 33.0 35.0 23.0 35.5 100-101 30.268672751569746 34.0 30.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08162296157681226 1101 2 0.05351145084345177 1101 3 0.13059304464215415 1101 4 -0.11808149942322643 1101 5 0.019758722426722386 1101 6 -0.11834295632984038 1101 7 0.039865380278619966 1101 8 0.01231233158598144 1101 9 0.18919348817828308 1101 10-11 0.33563442942475774 1101 12-13 0.3804868573178908 1101 14-15 0.34036145668051887 1101 16-17 0.2702561532823182 1101 18-19 0.29063061274044344 1101 20-21 0.2851492385344727 1101 22-23 0.29649810732866655 1101 24-25 0.3826887230979068 1101 26-27 0.33866259997474657 1101 28-29 0.2851254146953295 1101 30-31 0.3907696063659998 1101 32-33 0.49351931862776155 1101 34-35 0.49874429945144527 1101 36-37 0.4433642028791027 1101 38-39 0.45603036931449736 1101 40-41 0.2085417032866843 1101 42-43 0.38083535946033464 1101 44-45 0.4627863242501107 1101 46-47 0.5873164901011094 1101 48-49 0.5946990649994461 1101 50-51 0.43082281622733376 1101 52-53 0.590557119700037 1101 54-55 0.6230125707508876 1101 56-57 0.6222634807581855 1101 58-59 0.5255938214952991 1101 60-61 0.5428398858749404 1101 62-63 0.5553275841917582 1101 64-65 0.6278167942826371 1101 66-67 0.5079545293835466 1101 68-69 0.4932662092225115 1101 70-71 0.5616675722314923 1101 72-73 0.5896042717408037 1101 74-75 0.6590361389586192 1101 76-77 0.7228115275044189 1101 78-79 0.6525845198871068 1101 80-81 0.6534816250522795 1101 82-83 0.5658177711629833 1101 84-85 0.6315437970030757 1101 86-87 0.47342215308798785 1101 88-89 0.5632052168041923 1101 90-91 0.5761115680837605 1101 92-93 0.5351705176601769 1101 94-95 0.5013932801909817 1101 96-97 0.5317161070280214 1101 98-99 0.48685859001202303 1101 100-101 0.5853913559744903 1102 1 0.25458837884747254 1102 2 0.271906653739002 1102 3 0.35093527050582196 1102 4 0.16873636726985097 1102 5 0.2516840467804897 1102 6 0.15644163315218407 1102 7 0.28717106063371745 1102 8 0.3123226856965644 1102 9 0.4404990064690395 1102 10-11 0.4539402718441963 1102 12-13 0.5028443082259031 1102 14-15 0.6853289087589971 1102 16-17 0.6719945184132783 1102 18-19 0.6447873379603593 1102 20-21 0.7050534405026596 1102 22-23 0.6372046178133317 1102 24-25 0.6580247815130988 1102 26-27 0.732810951870448 1102 28-29 0.7272099222448247 1102 30-31 0.6230291884200696 1102 32-33 0.5654184875978459 1102 34-35 0.5717160192241977 1102 36-37 0.6763947159928136 1102 38-39 0.7115135461356985 1102 40-41 0.7775687320170874 1102 42-43 0.8054428036156622 1102 44-45 0.7265629185580522 1102 46-47 0.8154074171991681 1102 48-49 0.7783764849351584 1102 50-51 0.6449559520822703 1102 52-53 0.6340612658460572 1102 54-55 0.7654795051238281 1102 56-57 0.72299209501805 1102 58-59 0.6781425083239796 1102 60-61 0.684067703318469 1102 62-63 0.6989998720900488 1102 64-65 0.7168452635849505 1102 66-67 0.8017321591025492 1102 68-69 0.8463031148740541 1102 70-71 0.8216289018793574 1102 72-73 0.6886300849886666 1102 74-75 0.6928490626893407 1102 76-77 0.7280223461994382 1102 78-79 0.6213072278021414 1102 80-81 0.3985217809941801 1102 82-83 0.511194210609446 1102 84-85 0.6444155320819007 1102 86-87 0.6791853410736266 1102 88-89 0.8376454195646872 1102 90-91 0.7604856395901436 1102 92-93 0.7399399361193062 1102 94-95 0.7050192446988888 1102 96-97 0.7192549349427004 1102 98-99 0.7469617259569965 1102 100-101 0.4983425475975629 1103 1 0.09607809351236796 1103 2 0.23304813840476157 1103 3 0.29578683278042206 1103 4 0.20126572302992685 1103 5 0.18440381828160213 1103 6 0.12315854841717311 1103 7 0.2031429021847444 1103 8 0.3134009869573475 1103 9 0.39108107276834403 1103 10-11 0.42106831200461414 1103 12-13 0.4748463422122171 1103 14-15 0.5007230116411989 1103 16-17 0.3801463316904474 1103 18-19 0.504308846283827 1103 20-21 0.505847200059506 1103 22-23 0.46236113378228794 1103 24-25 0.5046896209580112 1103 26-27 0.5123704712149575 1103 28-29 0.3854966133385602 1103 30-31 0.44451443120014034 1103 32-33 0.430863440138161 1103 34-35 0.41275133268889164 1103 36-37 0.2251436701487819 1103 38-39 0.19118450196501868 1103 40-41 0.13841197081573853 1103 42-43 0.20555207628523675 1103 44-45 0.41212269756038467 1103 46-47 0.5058772536147345 1103 48-49 0.4342252775262594 1103 50-51 0.3887512118843546 1103 52-53 0.419921093894871 1103 54-55 0.3679091090013955 1103 56-57 0.30275934062550647 1103 58-59 0.49301729554881035 1103 60-61 0.3096861938409603 1103 62-63 0.35549239971476254 1103 64-65 0.48962843487256436 1103 66-67 0.42645086312148095 1103 68-69 0.41247438806293957 1103 70-71 0.4551651529973313 1103 72-73 0.3911667977205511 1103 74-75 0.4639282294762168 1103 76-77 0.2825306552499569 1103 78-79 0.35126308597126865 1103 80-81 0.34507915094888375 1103 82-83 0.24874325708819356 1103 84-85 0.33271434054098137 1103 86-87 0.4137591415987103 1103 88-89 0.4354877351355029 1103 90-91 0.44183679795256836 1103 92-93 0.493699565097117 1103 94-95 0.33684067223993 1103 96-97 0.36403761576552895 1103 98-99 0.2904381836471934 1103 100-101 0.4158579106857623 1104 1 0.12920132049097788 1104 2 0.08018978886853745 1104 3 0.20541772558633653 1104 4 0.11090458447129237 1104 5 0.10117142099731069 1104 6 -0.02755305072858505 1104 7 0.04031499283593831 1104 8 0.0780035114210591 1104 9 0.15046414101173156 1104 10-11 0.2811581982124949 1104 12-13 0.3598290502732908 1104 14-15 0.37301208186081425 1104 16-17 0.32592853294629975 1104 18-19 0.4067360926421699 1104 20-21 0.42573970316727383 1104 22-23 0.2345888455089309 1104 24-25 0.3052553940750258 1104 26-27 0.3567227008391498 1104 28-29 0.38777484621396496 1104 30-31 0.26393957383871935 1104 32-33 0.29016562673172785 1104 34-35 0.21638263986385198 1104 36-37 0.2158159839217717 1104 38-39 0.22306143435911707 1104 40-41 0.1727069856374044 1104 42-43 0.19713679881734691 1104 44-45 0.19367331433277002 1104 46-47 0.24370755355417373 1104 48-49 0.3064984104749726 1104 50-51 0.2578229455406813 1104 52-53 0.28426105182047223 1104 54-55 0.27530440113157084 1104 56-57 0.2689217254787266 1104 58-59 0.3177678772591932 1104 60-61 0.3026542714873415 1104 62-63 0.21532450056581354 1104 64-65 0.33077806990905856 1104 66-67 0.3358036505501545 1104 68-69 0.33389049426891404 1104 70-71 0.2558544399638265 1104 72-73 0.35184875354243417 1104 74-75 0.3677364403581862 1104 76-77 0.26758428416653857 1104 78-79 0.37786478169689275 1104 80-81 0.37433041796192157 1104 82-83 0.32645777275453725 1104 84-85 0.4457717465907649 1104 86-87 0.49332397441673237 1104 88-89 0.4091860585346012 1104 90-91 0.37318262079386955 1104 92-93 0.4541532195133442 1104 94-95 0.546143655697044 1104 96-97 0.5112458043353207 1104 98-99 0.4665653471317093 1104 100-101 0.43009945596354626 1105 1 0.27789592236778304 1105 2 0.18551358334962487 1105 3 0.22048787331466713 1105 4 0.2666827132457996 1105 5 0.07354343623470072 1105 6 0.13377743333719394 1105 7 0.07771348767938235 1105 8 0.1470809061994629 1105 9 0.15879267394864627 1105 10-11 0.15258252228570512 1105 12-13 0.1668586888498993 1105 14-15 0.18100799378128585 1105 16-17 0.28170480350284066 1105 18-19 0.29200414058419 1105 20-21 0.2938954218330778 1105 22-23 0.23300564379936617 1105 24-25 0.2312667292046342 1105 26-27 0.16674964499970457 1105 28-29 0.1982291805073828 1105 30-31 0.11093390627390676 1105 32-33 0.17188262013505806 1105 34-35 0.15930747494700626 1105 36-37 0.20025898308557544 1105 38-39 0.2217086516777087 1105 40-41 0.15638254078002234 1105 42-43 0.09349469159817403 1105 44-45 0.09505211206574415 1105 46-47 0.1875001764288342 1105 48-49 0.2824400624307444 1105 50-51 0.10624810581197863 1105 52-53 0.17492536474438225 1105 54-55 0.14279301954650947 1105 56-57 0.19158641004622723 1105 58-59 0.14356551732237932 1105 60-61 0.10727937601210158 1105 62-63 0.085611595242014 1105 64-65 0.2180121124622545 1105 66-67 0.15216525976691742 1105 68-69 0.12515686981476648 1105 70-71 0.06922246003258437 1105 72-73 0.09089734594233079 1105 74-75 0.14646356104943692 1105 76-77 0.11065963490518271 1105 78-79 0.23506258899968913 1105 80-81 0.10161277418230696 1105 82-83 0.05535492038994505 1105 84-85 0.005273744175077866 1105 86-87 0.015635597570131665 1105 88-89 -0.01746264535836417 1105 90-91 0.08628119925712241 1105 92-93 0.21753517621685603 1105 94-95 0.20329314933264442 1105 96-97 0.11560890012644975 1105 98-99 0.15562415973043286 1105 100-101 0.17548828807355576 1106 1 0.24270347476073084 1106 2 0.221774297064961 1106 3 0.21965739401453988 1106 4 0.2647584146281545 1106 5 0.2449302301942069 1106 6 0.3456028907218922 1106 7 0.20520057798513847 1106 8 0.1978522937032352 1106 9 0.29759912613398143 1106 10-11 0.2065647415109524 1106 12-13 0.17556523508523725 1106 14-15 0.17660787311334758 1106 16-17 0.19762729210149388 1106 18-19 0.30443577687684353 1106 20-21 0.3244464129072 1106 22-23 0.24255509103376482 1106 24-25 0.23354060992082992 1106 26-27 0.33646163903728876 1106 28-29 0.24950204974237522 1106 30-31 0.31672774156218253 1106 32-33 0.17059218306870605 1106 34-35 0.2378055140931039 1106 36-37 0.1540119332390688 1106 38-39 0.1441113631571298 1106 40-41 0.1193073185203346 1106 42-43 0.15076227273848275 1106 44-45 0.30628061809026974 1106 46-47 0.16724499924217895 1106 48-49 0.19241717101414224 1106 50-51 0.23431937087329402 1106 52-53 0.21057883483470619 1106 54-55 0.20244237273089283 1106 56-57 0.237592124027735 1106 58-59 0.4124818128168499 1106 60-61 0.27953675361400343 1106 62-63 0.25123543070773735 1106 64-65 0.14387231511339849 1106 66-67 0.18451491284660904 1106 68-69 0.1706948181050052 1106 70-71 0.25516500080237137 1106 72-73 0.26668426056976813 1106 74-75 0.260903963512483 1106 76-77 0.360832296520357 1106 78-79 0.295106952210169 1106 80-81 0.3767509212197311 1106 82-83 0.3055382291756388 1106 84-85 0.34956384408103247 1106 86-87 0.3828061120059232 1106 88-89 0.4062986450787065 1106 90-91 0.4293990456894292 1106 92-93 0.35836743004540494 1106 94-95 0.16179935130947598 1106 96-97 0.20142757881258788 1106 98-99 0.24601437852978236 1106 100-101 0.24369193295492764 1107 1 0.2610454049674189 1107 2 0.28188324285579114 1107 3 0.2765105501358178 1107 4 0.3245741787012122 1107 5 0.2632166031347367 1107 6 0.25093531728924745 1107 7 0.3012905513042625 1107 8 0.2985695848648078 1107 9 0.26334238195042303 1107 10-11 0.30961677658030595 1107 12-13 0.28347116793189997 1107 14-15 0.4215250526249008 1107 16-17 0.47047772792482334 1107 18-19 0.36090103618201397 1107 20-21 0.480083735073741 1107 22-23 0.4387117330722745 1107 24-25 0.5162477749339516 1107 26-27 0.49556560073892086 1107 28-29 0.5478248227327143 1107 30-31 0.4433149724943277 1107 32-33 0.5155204740334867 1107 34-35 0.5164657004174487 1107 36-37 0.4560453291803128 1107 38-39 0.31103517741110664 1107 40-41 0.43515205001808965 1107 42-43 0.5304640573985182 1107 44-45 0.43325674988549423 1107 46-47 0.4665162619450385 1107 48-49 0.5802912287160495 1107 50-51 0.5302910005218564 1107 52-53 0.4450445546627648 1107 54-55 0.4899836892640721 1107 56-57 0.5797090834521867 1107 58-59 0.5879400263896954 1107 60-61 0.4622686181827902 1107 62-63 0.5929815621147299 1107 64-65 0.5824634631674854 1107 66-67 0.6371469885330114 1107 68-69 0.6102291009544132 1107 70-71 0.5929183720574471 1107 72-73 0.5717548986215419 1107 74-75 0.6489948953617883 1107 76-77 0.7101596519811011 1107 78-79 0.7216702868242137 1107 80-81 0.7172071869414367 1107 82-83 0.7454751348634971 1107 84-85 0.7027246509064113 1107 86-87 0.7652964698080993 1107 88-89 0.688692882116797 1107 90-91 0.7449315185768199 1107 92-93 0.5698930191867007 1107 94-95 0.5797369183296084 1107 96-97 0.6852369865989125 1107 98-99 0.7295550364081471 1107 100-101 0.7945060811817122 1108 1 0.1605777248907856 1108 2 0.05142673809297804 1108 3 0.13986975293867943 1108 4 -0.03913463940106254 1108 5 0.1573945492778961 1108 6 0.14904999358260795 1108 7 0.08100816114843923 1108 8 0.14331378403538508 1108 9 0.16972592416600918 1108 10-11 0.20280775563927733 1108 12-13 0.18631307622501936 1108 14-15 0.15217090655872312 1108 16-17 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-0.6840208606938702 2316 60-61 -0.7852434203404783 2316 62-63 -0.5968274594072014 2316 64-65 -0.9281140583708236 2316 66-67 -0.5759905618322421 2316 68-69 -0.4876711185234228 2316 70-71 -0.9016143366921838 2316 72-73 -0.6214691325639663 2316 74-75 -0.4039352975821586 2316 76-77 -0.4664348381423906 2316 78-79 -0.4772716334430882 2316 80-81 -0.3413671898397723 2316 82-83 -0.4043709030701308 2316 84-85 -0.318436943747507 2316 86-87 -0.3480130594610742 2316 88-89 -0.32080362916830296 2316 90-91 -0.3058694117471603 2316 92-93 -0.3070699971594806 2316 94-95 -0.26414063269080046 2316 96-97 -0.24157861925820256 2316 98-99 -0.25807722355844476 2316 100-101 -0.13677770038870207 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 42.0 7 546.0 8 890.0 9 745.0 10 826.0 11 1032.0 12 1265.0 13 1622.0 14 1635.0 15 1822.0 16 2226.0 17 3023.0 18 3864.0 19 5165.0 20 6772.0 21 9187.0 22 10860.0 23 10790.0 24 9626.0 25 8539.0 26 8296.0 27 8691.0 28 9822.0 29 11440.0 30 13640.0 31 16650.0 32 20799.0 33 27985.0 34 40717.0 35 65381.0 36 124145.0 37 269742.0 38 424259.0 39 164108.0 40 200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.54970991632934 19.897026710397533 13.831662070986736 22.721601302286388 2 14.31224555001621 21.83716289385573 41.77523588004226 22.075355676085803 3 17.015391576029284 28.73013861669386 31.327637126045495 22.926832681231357 4 11.30405106529856 18.574916838534993 41.36306286066111 28.75796923550534 5 11.779270542378336 39.61424274674214 35.96508889861492 12.641397812264596 6 27.11331959423269 40.39109015954408 19.724756734737664 12.770833511485572 7 24.75852273831522 34.652696421588786 24.283147782918064 16.30563305717793 8 24.583842848735923 36.0418951874019 21.768052781779186 17.606209182082992 9 26.59573227566617 16.143546911306615 21.078428705028866 36.18229210799835 10-11 22.926871550810706 27.291769833008512 30.496022475945562 19.285336140235223 12-13 24.588273980781324 25.356531216547868 29.83881562836951 20.2163791743013 14-15 21.857958118805644 26.370988367889687 27.930902326188843 23.840151187115826 16-17 20.6490364619976 29.629541813172587 28.16734597734836 21.554075747481445 18-19 20.587039482941307 28.495910531557044 30.598910252473466 20.318139733028183 20-21 21.924347360327996 27.634171957464243 30.388703567372254 20.052777114835507 22-23 21.631892645331412 27.654384138723977 29.96898984959805 20.744733366346562 24-25 21.036838255129037 27.801544366126564 30.226306464866177 20.935310913878226 26-27 20.962597358578865 28.1532363200459 30.03024830664678 20.85391801472846 28-29 20.972431362153312 27.998263307194836 30.265681348743307 20.763623981908545 30-31 21.316776965576324 28.00627044054004 30.006693341563317 20.67025925232032 32-33 21.075435747419256 28.14382988184425 29.99689820756822 20.783836163168274 34-35 21.165224475707678 28.186469810386416 29.942247579008253 20.706058134897653 36-37 21.36108828603035 27.990139565111598 29.784203869388886 20.864568279469168 38-39 21.177468393201554 28.31800446689206 29.72006906346858 20.784458076437808 40-41 21.133351420644257 28.1073313468387 29.933152097441372 20.826165135075676 42-43 21.131680028732394 28.109663521599437 29.620290853288328 21.138365596379842 44-45 21.159471777964526 28.397881452447344 29.411405733884866 21.031241035703264 46-47 21.209521658737913 28.320836101174525 29.139585875244826 21.330056364842736 48-49 21.23448421154102 28.45852337348049 29.084130225731545 21.222862189246946 50-51 21.295709653570988 28.605522745311745 28.747668796978747 21.35109880413852 52-53 21.09607549405179 28.704756781381164 28.758008105084688 21.441159619482367 54-55 21.24195302533597 28.604551005828107 28.8069060359015 21.346589932934428 56-57 21.225705541169493 28.62647344857904 28.650455979035307 21.49736503121616 58-59 21.21272310166805 28.483083570372987 28.66320520106067 21.640988126898293 60-61 21.24230285155008 28.531009761706155 28.853121965743462 21.3735654210003 62-63 21.083015315391656 28.580918301585957 28.885422586179686 21.4506437968427 64-65 21.168256302896637 28.34676795560783 28.840372743718095 21.644602997777437 66-67 21.100390017359157 28.509553753907362 28.713346958416547 21.676709270316934 68-69 21.170549608078034 28.406277281586 28.76243923713009 21.660733873205878 70-71 21.15422438475286 28.119186568539117 28.8037575999745 21.922831446733518 72-73 21.09739705974954 28.28480984613088 28.600353091258775 22.0174400028608 74-75 21.07022722378694 28.266191317624322 28.560161946215384 22.103419512373353 76-77 20.993887369952105 28.14107014171071 28.808421949495976 22.056620538841205 78-79 20.963491358903816 28.185964505854926 28.654615024025286 22.195929111215975 80-81 20.99213823888155 28.302534374312494 28.46858521727706 22.236742169528892 82-83 21.161920561463297 28.06336985259878 28.535207676275487 22.239501909662433 84-85 21.131213593780245 27.978167734673143 28.49171261698772 22.398906054558896 86-87 20.94537813492875 28.09671995167734 28.674049813698105 22.283852099695807 88-89 21.118658719651602 27.865329345832752 28.644197976760655 22.371813957754984 90-91 21.16666265014347 27.88515283129903 28.640816323357587 22.307368195199917 92-93 21.279034604031708 27.796141494597514 28.52319697625768 22.401626925113092 94-95 21.113333587281254 27.625892737063623 28.64233223695207 22.618441438703062 96-97 21.129386723551 27.486856251744275 28.748990362676498 22.63476666202823 98-99 21.175486044654928 27.38062569139264 28.63277032043304 22.811117943519392 100-101 21.38067855402055 27.2486634695142 28.757697148449918 22.61296082801533 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 109.0 1 91.5 2 130.0 3 181.5 4 157.5 5 171.0 6 273.5 7 558.5 8 1067.5 9 1359.0 10 1195.0 11 1043.0 12 1090.0 13 1198.0 14 1331.0 15 1460.0 16 1567.0 17 1684.5 18 1829.0 19 2027.5 20 2292.0 21 2692.0 22 3259.5 23 4124.5 24 5200.0 25 6442.5 26 8452.0 27 11190.0 28 14178.5 29 18008.0 30 22882.0 31 27097.0 32 30709.5 33 34035.5 34 36258.0 35 38728.0 36 42281.5 37 44362.5 38 44332.0 39 44480.5 40 44454.0 41 44109.0 42 44050.5 43 44776.0 44 45861.0 45 46800.5 46 46888.0 47 46410.0 48 46308.5 49 45842.5 50 44589.5 51 42749.5 52 39987.5 53 36990.5 54 34233.0 55 30658.0 56 26688.0 57 22758.5 58 19874.5 59 17011.5 60 13590.5 61 10910.0 62 8476.5 63 6029.0 64 4203.5 65 3117.0 66 2321.5 67 1698.0 68 1277.5 69 940.0 70 704.0 71 570.5 72 439.5 73 339.0 74 283.5 75 218.0 76 160.5 77 111.0 78 88.5 79 77.5 80 52.0 81 44.0 82 42.0 83 28.0 84 19.0 85 18.5 86 19.5 87 17.5 88 12.0 89 9.5 90 6.5 91 4.0 92 2.5 93 2.0 94 1.0 95 0.0 96 1.0 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.1660873803691521E-4 48-49 1.5547831738255363E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1286353.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.77406294640905 #Duplication Level Percentage of deduplicated Percentage of total 1 69.75952930685956 38.21012849365793 2 15.699582459782999 17.198598357689868 3 6.212515983473223 10.208542246030039 4 3.0134001106620736 6.602246693764817 5 1.6867161929820715 4.619414946356371 6 1.0256742957567413 3.3708209062976113 7 0.6617108959591617 2.5371215987314297 8 0.4351590531649048 1.9068343495803404 9 0.3011658817144185 1.4846471066102704 >10 1.152564525596005 10.003290816722977 >50 0.035685384030153526 1.3528426215613034 >100 0.015724128533987566 1.5636773992227238 >500 1.4294537118986214E-4 0.04408120401571091 >1k 2.858907423797243E-4 0.4723657261576979 >5k 1.4294537118986214E-4 0.42538753360099 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5433 0.4223568491697069 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3782 0.2940094981704089 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2251 0.1749908462140641 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.773915869127682E-5 0.0 12-13 0.0 0.0 0.0 3.1095663476510727E-4 0.0 14-15 0.0 0.0 0.0 5.053045314932993E-4 0.0 16-17 0.0 0.0 0.0 8.551307456040449E-4 0.0 18-19 0.0 0.0 0.0 0.0012826961184060673 0.0 20-21 0.0 0.0 0.0 0.0017102614912080899 0.0 22-23 0.0 0.0 0.0 0.0029152184509228804 0.0 24-25 0.0 0.0 0.0 0.0049364365768960776 0.0 26-27 0.0 0.0 0.0 0.006996524282214913 0.0 28-29 0.0 0.0 0.0 0.012749222025369397 0.0 30-31 0.0 0.0 0.0 0.029929576096141573 0.0 32-33 0.0 0.0 0.0 0.06615602404627657 0.0 34-35 0.0 0.0 0.0 0.12037908723344215 0.0 36-37 3.886957934563841E-5 0.0 0.0 0.21063425047401452 0.0 38-39 7.773915869127682E-5 0.0 0.0 0.35227499760952086 0.0 40-41 7.773915869127682E-5 0.0 0.0 0.5037497483194737 0.0 42-43 7.773915869127682E-5 0.0 0.0 0.6496661491830003 0.0 44-45 7.773915869127682E-5 0.0 0.0 0.8216640377874502 0.0 46-47 7.773915869127682E-5 0.0 0.0 1.0395280300197536 0.0 48-49 7.773915869127682E-5 0.0 0.0 1.2708797662849933 0.0 50-51 7.773915869127682E-5 0.0 0.0 1.5051078514218104 0.0 52-53 7.773915869127682E-5 0.0 0.0 1.7298517591982916 0.0 54-55 7.773915869127682E-5 0.0 0.0 1.9577441029017697 0.0 56-57 7.773915869127682E-5 0.0 0.0 2.223262199411826 0.0 58-59 7.773915869127682E-5 0.0 0.0 2.5295544846554563 0.0 60-61 7.773915869127682E-5 0.0 0.0 2.847857469916889 0.0 62-63 7.773915869127682E-5 0.0 0.0 3.164061497893657 0.0 64-65 7.773915869127682E-5 0.0 0.0 3.4694209132329927 0.0 66-67 7.773915869127682E-5 0.0 0.0 3.806420166159678 0.0 68-69 7.773915869127682E-5 0.0 0.0 4.198070047646331 0.0 70-71 7.773915869127682E-5 0.0 0.0 4.600136976397614 0.0 72-73 7.773915869127682E-5 0.0 0.0 4.995168511287337 0.0 74-75 7.773915869127682E-5 0.0 0.0 5.385457957496892 0.0 76-77 7.773915869127682E-5 0.0 0.0 5.792344714087035 0.0 78-79 7.773915869127682E-5 0.0 0.0 6.249567575929779 0.0 80-81 7.773915869127682E-5 0.0 0.0 6.730345402855981 3.886957934563841E-5 82-83 7.773915869127682E-5 0.0 0.0 7.22282297316522 7.773915869127682E-5 84-85 7.773915869127682E-5 0.0 0.0 7.718371240242764 7.773915869127682E-5 86-87 7.773915869127682E-5 0.0 0.0 8.226435511869603 7.773915869127682E-5 88-89 7.773915869127682E-5 0.0 0.0 8.77818919068094 1.1660873803691522E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2090 0.0 62.045452 1 GTATCAA 4545 0.0 50.68757 1 TCAACGC 5810 0.0 39.079174 4 ATCAACG 5850 0.0 38.89316 3 CAACGCA 5915 0.0 38.385464 5 TATCAAC 5975 0.0 38.0795 2 AACGCAG 6045 0.0 37.638546 6 ACGCAGA 6730 0.0 33.737 7 CGCAGAG 6770 0.0 33.397343 8 GCAGAGT 7510 0.0 29.600533 9 GTACATG 5740 0.0 29.377178 1 TACATGG 5805 0.0 28.229973 2 ACATGGG 5610 0.0 27.602497 3 TATGCCG 70 0.008391935 27.142859 5 CGTATAG 150 1.0602762E-6 25.333334 1 CATGGGG 2850 0.0 24.0 4 AGAGTAC 7065 0.0 23.430645 10-11 GAGTACT 4750 0.0 23.3 12-13 CAGAGTA 7300 0.0 23.294521 10-11 CATGGGA 2900 0.0 22.275862 4 >>END_MODULE