FastQCFastQC Report
Fri 10 Feb 2017
SRR2031525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1286353
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67610.5255944519117225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51730.40214466790997494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30040.23352843270859555No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT26400.2052313789449708No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24650.065.63211
GTATCAA48650.046.0672531
TACACCG1650.037.422825
TCAACGC59600.037.2972374
ATCAACG59750.037.2036063
CAACGCA60050.037.0177425
AACGCAG60800.036.561116
TATCAAC62350.035.8807532
ACGCAGA69250.032.0312737
CGCAGAG70150.031.6203238
GTACATG50550.028.333461
TACATGG50150.027.6559752
GCAGAGT80050.027.3537379
ACATGGG49200.026.4522463
TCGATCG559.482305E-525.90860740-41
GAGTACT52450.024.9942812-13
CAGAGTA76150.024.2948710-11
CATGGGA29250.023.8708884
ATGGGAT14750.023.8296015
GTACTTT58000.022.68448314-15
Produced by FastQC (version 0.11.5)