##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031525_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1286353
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.800209584771835	34.0	31.0	34.0	31.0	34.0
2	32.94160545355746	34.0	33.0	34.0	31.0	34.0
3	33.04573550184125	34.0	33.0	34.0	31.0	34.0
4	36.410273851734324	37.0	37.0	37.0	35.0	37.0
5	36.34243555229396	37.0	37.0	37.0	35.0	37.0
6	36.35759779780511	37.0	37.0	37.0	35.0	37.0
7	36.383047266185876	37.0	37.0	37.0	35.0	37.0
8	36.4128236961394	37.0	37.0	37.0	35.0	37.0
9	38.27753579305214	39.0	39.0	39.0	37.0	39.0
10-11	38.239449047034526	39.0	39.0	39.0	37.0	39.0
12-13	38.24933552454109	39.0	39.0	39.0	37.0	39.0
14-15	39.802524268221866	41.0	40.0	41.0	37.5	41.0
16-17	39.802064052402415	41.0	40.0	41.0	38.0	41.0
18-19	39.83175185971503	41.0	40.0	41.0	38.0	41.0
20-21	39.819792856237754	41.0	40.0	41.0	38.0	41.0
22-23	39.793562886703725	41.0	40.0	41.0	37.5	41.0
24-25	39.74903311921378	41.0	40.0	41.0	37.0	41.0
26-27	39.64392744448841	41.0	40.0	41.0	37.0	41.0
28-29	39.561148844834975	41.0	40.0	41.0	37.0	41.0
30-31	39.41227291420007	41.0	39.0	41.0	36.5	41.0
32-33	38.490507660028	40.0	38.0	41.0	35.0	41.0
34-35	38.687209498481366	40.0	38.0	41.0	35.0	41.0
36-37	38.897067523455846	40.0	38.5	41.0	35.0	41.0
38-39	38.85917551403075	40.0	38.5	41.0	35.0	41.0
40-41	38.74993839171674	40.0	38.0	41.0	35.0	41.0
42-43	38.56672313120893	40.0	38.0	41.0	35.0	41.0
44-45	38.3963756449435	40.0	38.0	41.0	34.5	41.0
46-47	38.138021600602634	40.0	38.0	41.0	33.5	41.0
48-49	37.946223159583724	40.0	38.0	41.0	33.5	41.0
50-51	37.951819601617906	40.0	38.0	41.0	33.0	41.0
52-53	38.06078968992182	40.5	38.0	41.0	34.0	41.0
54-55	37.98152334545805	40.5	38.0	41.0	34.0	41.0
56-57	37.849324407841394	40.0	38.0	41.0	33.5	41.0
58-59	37.70953152050798	40.0	37.5	41.0	33.0	41.0
60-61	37.50770978106321	40.0	37.0	41.0	33.0	41.0
62-63	37.23124912057577	40.0	36.5	41.0	32.5	41.0
64-65	36.99831344895219	39.0	36.0	41.0	32.0	41.0
66-67	36.71341808974675	39.0	35.5	41.0	32.0	41.0
68-69	36.36393820358798	39.0	35.0	41.0	31.0	41.0
70-71	35.97040820054838	37.5	35.0	40.0	31.0	41.0
72-73	35.59244701881987	37.0	35.0	39.5	31.0	41.0
74-75	35.16629572131444	36.5	35.0	39.0	30.0	41.0
76-77	33.68299059433919	35.0	33.5	37.5	28.0	39.0
78-79	34.3554420909346	35.5	34.5	37.5	29.5	39.0
80-81	34.18370696068653	35.0	35.0	37.0	30.0	39.0
82-83	33.89950892173455	35.0	35.0	37.0	29.5	39.0
84-85	33.59340126699281	35.0	34.5	36.0	29.0	37.5
86-87	33.34507829499368	35.0	34.0	36.0	29.0	37.0
88-89	33.12990835330582	35.0	34.0	36.0	29.0	37.0
90-91	32.96179003741585	35.0	34.0	35.0	29.0	36.0
92-93	32.81266262060259	35.0	34.0	35.0	29.0	36.0
94-95	32.70227418134836	35.0	34.0	35.0	29.0	36.0
96-97	32.60324071230836	35.0	34.0	35.0	27.0	36.0
98-99	32.54217582576478	35.0	34.0	35.0	27.0	35.5
100-101	31.418960425326482	34.5	31.5	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4309769089407851
1101	2	0.33365656067624627
1101	3	0.3066918929930651
1101	4	0.16234786669406276
1101	5	0.21955863045414503
1101	6	0.3174724396377755
1101	7	0.272768806323441
1101	8	0.22443367952566717
1101	9	0.2620151908591879
1101	10-11	0.25012009741740115
1101	12-13	0.17545585188239698
1101	14-15	0.2500450049902625
1101	16-17	0.18984949591049372
1101	18-19	0.20703736341607026
1101	20-21	0.19530887473111136
1101	22-23	0.19327057993834273
1101	24-25	0.16572029432563085
1101	26-27	0.12486029409668475
1101	28-29	0.13665214914368562
1101	30-31	0.07852054494946259
1101	32-33	0.11935034172380199
1101	34-35	-0.2611965135901144
1101	36-37	-0.046999960613860026
1101	38-39	0.09002967300786935
1101	40-41	0.11616941377736367
1101	42-43	0.047474202277179245
1101	44-45	0.036396167140104296
1101	46-47	0.12307587697863909
1101	48-49	0.14776034766779134
1101	50-51	0.2863006850046119
1101	52-53	0.28631927995514417
1101	54-55	0.2742293401733775
1101	56-57	0.2554814989051053
1101	58-59	0.11321676101383105
1101	60-61	0.195171293585517
1101	62-63	0.2858651562785539
1101	64-65	0.30641199149916787
1101	66-67	0.28119677738453674
1101	68-69	0.06353175236466768
1101	70-71	0.0190997166411222
1101	72-73	0.24822669931091212
1101	74-75	0.303207594395019
1101	76-77	0.4554379289473687
1101	78-79	0.26555820692441046
1101	80-81	0.24879883462757846
1101	82-83	0.21854926356827775
1101	84-85	0.16952728946542805
1101	86-87	0.1532745284372936
1101	88-89	0.2655523932841888
1101	90-91	0.12043145189795013
1101	92-93	0.06376686550684951
1101	94-95	0.13874987890473278
1101	96-97	0.47585432478266654
1101	98-99	0.26377228193853597
1101	100-101	0.014197408774435871
1102	1	0.319969589034784
1102	2	0.28464785421978434
1102	3	0.18844119746693622
1102	4	0.12413894775324508
1102	5	0.10668757064340184
1102	6	0.1966399691469931
1102	7	0.1975416911587189
1102	8	0.1792875064949726
1102	9	0.20407944036624315
1102	10-11	0.1789063255499741
1102	12-13	0.25008085300784444
1102	14-15	0.3619841948486382
1102	16-17	0.3511665387765106
1102	18-19	0.34017209290367845
1102	20-21	0.32264192595245333
1102	22-23	0.24217349439538793
1102	24-25	0.302749069749467
1102	26-27	0.29251472790689803
1102	28-29	0.32729833567974254
1102	30-31	0.35545811416444195
1102	32-33	0.5011356604953576
1102	34-35	0.1888336483841826
1102	36-37	0.37354687147848153
1102	38-39	0.3836351093661037
1102	40-41	0.4304211717252642
1102	42-43	0.4863905770779766
1102	44-45	0.5087461900992238
1102	46-47	0.5223483881655895
1102	48-49	0.5972885603682343
1102	50-51	0.5076501857564111
1102	52-53	0.551283220639597
1102	54-55	0.6143146040231215
1102	56-57	0.47256217993547267
1102	58-59	0.4452753382689991
1102	60-61	0.4730238510508329
1102	62-63	0.41286282435277855
1102	64-65	0.33007113417880873
1102	66-67	0.3900252322811326
1102	68-69	0.4036316470228485
1102	70-71	0.23491539546561313
1102	72-73	0.27375070734210283
1102	74-75	0.3807092375473431
1102	76-77	0.35450110702907267
1102	78-79	0.42990607802923364
1102	80-81	0.3429086962426382
1102	82-83	0.4340543190753152
1102	84-85	0.4347140686167492
1102	86-87	0.5748477680133988
1102	88-89	0.4904504278038928
1102	90-91	0.46119873650993526
1102	92-93	0.5030681431140636
1102	94-95	0.4763501562148278
1102	96-97	0.47469061278900426
1102	98-99	0.5287801150482778
1102	100-101	0.4213064060463516
1103	1	0.33985865744019605
1103	2	0.24784856972135572
1103	3	0.22326780373911959
1103	4	0.13077104146086782
1103	5	0.11040212013706707
1103	6	0.15939710252456507
1103	7	0.12039446516985208
1103	8	0.060173083945130657
1103	9	0.19411867754924828
1103	10-11	0.11304159285328552
1103	12-13	0.1594169944925028
1103	14-15	0.24571158297938922
1103	16-17	0.18759653214080174
1103	18-19	0.27739260010196887
1103	20-21	0.2401987621660595
1103	22-23	0.2574031473194225
1103	24-25	0.21195177596106163
1103	26-27	0.266006188049424
1103	28-29	0.27861068962221225
1103	30-31	0.2768935891940103
1103	32-33	0.5712306519843438
1103	34-35	0.2249801819521693
1103	36-37	0.27809917810552776
1103	38-39	0.3898380463846607
1103	40-41	0.4425800101892392
1103	42-43	0.477109612881911
1103	44-45	0.3667764690871351
1103	46-47	0.32799270021659055
1103	48-49	0.5282015461447571
1103	50-51	0.3397327136696262
1103	52-53	0.40214946800696083
1103	54-55	0.46555771587794936
1103	56-57	0.37565030491744267
1103	58-59	0.4067089379920361
1103	60-61	0.33533307467392603
1103	62-63	0.3792353323588955
1103	64-65	0.5125114128782116
1103	66-67	0.49529484365992005
1103	68-69	0.37881989894864176
1103	70-71	0.5636061429916523
1103	72-73	0.42666392392558095
1103	74-75	0.41430427231264133
1103	76-77	0.2833584683285082
1103	78-79	0.33248931278876626
1103	80-81	0.21694038892317735
1103	82-83	0.2577682915515851
1103	84-85	0.2900431849146017
1103	86-87	0.3182625569692874
1103	88-89	0.3896815521222905
1103	90-91	0.37367060691182985
1103	92-93	0.3852880732949586
1103	94-95	0.3826695786204155
1103	96-97	0.4696326946677729
1103	98-99	0.4047664541613969
1103	100-101	0.49452450419700966
1104	1	0.25244192968065704
1104	2	0.23768218461314206
1104	3	0.25519732912719206
1104	4	0.06650479341318771
1104	5	0.07643660839129751
1104	6	0.2363382621203911
1104	7	0.15609235826264012
1104	8	0.14344771499730768
1104	9	0.12952173113035315
1104	10-11	0.21114306545711514
1104	12-13	0.16496555535694313
1104	14-15	0.19471630041157795
1104	16-17	0.19773263643430994
1104	18-19	0.2437514118190549
1104	20-21	0.24794786171025862
1104	22-23	0.1465506626988855
1104	24-25	0.09043008533536323
1104	26-27	0.15597805876628712
1104	28-29	0.21839210995179315
1104	30-31	0.19297429553386536
1104	32-33	0.5921224219776562
1104	34-35	0.5185072593754327
1104	36-37	0.29933997433158055
1104	38-39	0.16228648931328848
1104	40-41	0.27464938512947157
1104	42-43	0.20383840834297473
1104	44-45	0.26872599575018086
1104	46-47	0.22389403257243856
1104	48-49	0.2149983140662144
1104	50-51	0.30577875644526387
1104	52-53	0.4074286889931571
1104	54-55	0.27303269436262667
1104	56-57	0.19129632219343762
1104	58-59	0.19526757121168004
1104	60-61	0.160340246445827
1104	62-63	0.21477265744734808
1104	64-65	0.15944787097912894
1104	66-67	0.15611943805637196
1104	68-69	0.2817117167537617
1104	70-71	0.1940606739577646
1104	72-73	0.17096794330529974
1104	74-75	0.2258026148364749
1104	76-77	0.1894010845909051
1104	78-79	0.21778040704178636
1104	80-81	0.16010927618292214
1104	82-83	0.24853901797239075
1104	84-85	0.258649550260607
1104	86-87	0.16752386446299283
1104	88-89	0.269168690744678
1104	90-91	0.33818667168089434
1104	92-93	0.342431158113115
1104	94-95	0.26337312944436775
1104	96-97	0.22324766614006109
1104	98-99	0.21419056087736976
1104	100-101	0.18183724126391354
1105	1	0.08364884225150604
1105	2	0.08431230632641729
1105	3	0.07409168943565447
1105	4	0.03760207112386382
1105	5	-0.0069639304919135725
1105	6	0.13969606199032114
1105	7	0.11230159360594882
1105	8	0.1051684248365703
1105	9	0.10676971031831073
1105	10-11	0.13959424077997795
1105	12-13	0.09976254504383064
1105	14-15	0.00335329753133351
1105	16-17	0.061263250575343875
1105	18-19	0.0856337866216208
1105	20-21	0.08959424283076345
1105	22-23	0.0789895737831614
1105	24-25	0.04776168433192396
1105	26-27	0.10972143886477426
1105	28-29	0.12759772177006568
1105	30-31	0.266360897782306
1105	32-33	0.4602930063872108
1105	34-35	0.2505206385448062
1105	36-37	0.16226480941473653
1105	38-39	0.17976117109346035
1105	40-41	0.022585834074938305
1105	42-43	0.02103064099186014
1105	44-45	0.009231431037740379
1105	46-47	0.004488662322508219
1105	48-49	0.028795582485805937
1105	50-51	0.14508564114283473
1105	52-53	0.17455123846820442
1105	54-55	0.18504793174997758
1105	56-57	0.18093489879848335
1105	58-59	0.12053561915369926
1105	60-61	0.07667955704695828
1105	62-63	0.10750330875274727
1105	64-65	-0.05654395286180147
1105	66-67	0.01077706139558643
1105	68-69	-0.015447067971692263
1105	70-71	0.2159077426189242
1105	72-73	0.17349883617903572
1105	74-75	0.055660461161842534
1105	76-77	0.0855397076513924
1105	78-79	0.11888976316924982
1105	80-81	0.1792563541351626
1105	82-83	0.22974685965222363
1105	84-85	0.3034307450947509
1105	86-87	0.2207450853122026
1105	88-89	0.26021579657018634
1105	90-91	0.25092289764020137
1105	92-93	0.21383132535272864
1105	94-95	0.2153233292027963
1105	96-97	0.14150837412110917
1105	98-99	0.028994983436135158
1105	100-101	0.054774154763869376
1106	1	0.23142660031229667
1106	2	0.13578285802730505
1106	3	0.06283834001326483
1106	4	0.05905753933318181
1106	5	-0.01229745310827468
1106	6	0.13909201251646408
1106	7	0.10303358657881745
1106	8	0.05874321233679325
1106	9	0.07954631493563369
1106	10-11	0.12454943393739626
1106	12-13	0.12317709262781307
1106	14-15	0.11295420608988849
1106	16-17	0.156420917425379
1106	18-19	0.16208437487778582
1106	20-21	0.17161933466260848
1106	22-23	0.0778336459709692
1106	24-25	0.10290409023755132
1106	26-27	0.1557839377556931
1106	28-29	0.08075230094064523
1106	30-31	0.10566208849087388
1106	32-33	0.4733407589469678
1106	34-35	0.24812218593358892
1106	36-37	0.029269937801863932
1106	38-39	0.027056257424270314
1106	40-41	0.11041718289398972
1106	42-43	0.08529092882037759
1106	44-45	-0.03488618470587568
1106	46-47	0.010264042515224503
1106	48-49	-0.07425838092341763
1106	50-51	-0.14189925762031663
1106	52-53	-0.02187557110714522
1106	54-55	0.057863575992520566
1106	56-57	-0.012969241176321589
1106	58-59	-0.1345556803334631
1106	60-61	0.07706888858210448
1106	62-63	0.07576125420374069
1106	64-65	-0.12238924947423158
1106	66-67	-0.12594822385985083
1106	68-69	-0.09509464662765765
1106	70-71	-0.15122160275754482
1106	72-73	-0.051923767763966566
1106	74-75	-0.1434639865161884
1106	76-77	-0.17846114412225234
1106	78-79	0.05535028878457382
1106	80-81	0.10989749125297976
1106	82-83	-0.07385081926460657
1106	84-85	-0.09780397177923561
1106	86-87	-0.0822985858321914
1106	88-89	-0.1406189415981487
1106	90-91	-0.06887161196886638
1106	92-93	0.01466748314379629
1106	94-95	0.12632114653284532
1106	96-97	0.08698527863372618
1106	98-99	0.16132263236342936
1106	100-101	0.15778517458534935
1107	1	0.19557944571450037
1107	2	0.22571936209637045
1107	3	0.1661199671584086
1107	4	0.12078433948901335
1107	5	0.06941255938206581
1107	6	0.1640265151852205
1107	7	0.07953085490166956
1107	8	0.09516535117105462
1107	9	0.11196966933610497
1107	10-11	0.14824695072841365
1107	12-13	0.11749452141076233
1107	14-15	0.12620361002066005
1107	16-17	0.16092139066618927
1107	18-19	0.13815726601098532
1107	20-21	0.18067617309270645
1107	22-23	0.14018864485313998
1107	24-25	0.19998940879488458
1107	26-27	0.1213337471875704
1107	28-29	0.19558515389328335
1107	30-31	0.262895510671207
1107	32-33	0.8562514777508881
1107	34-35	0.6102993346275909
1107	36-37	0.3165177379349373
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2316	92-93	-0.26485237913210113
2316	94-95	-0.2606570690355241
2316	96-97	-0.33408743259064266
2316	98-99	-0.3586906440063373
2316	100-101	-0.324975240434938
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	0.0
10	0.0
11	0.0
12	3.0
13	11.0
14	58.0
15	194.0
16	607.0
17	1302.0
18	2440.0
19	3735.0
20	5580.0
21	8181.0
22	10640.0
23	10884.0
24	9022.0
25	7531.0
26	6955.0
27	7552.0
28	8972.0
29	10928.0
30	12217.0
31	14490.0
32	18290.0
33	24355.0
34	34180.0
35	53569.0
36	101398.0
37	261005.0
38	490825.0
39	181227.0
40	199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.81551500921362	20.133186870334313	14.392247322132704	22.65905079831936
2	14.172548281847986	21.89453439296989	41.98987369718887	21.94304362799325
3	16.55727471386159	28.64353719391178	32.22365866912115	22.57552942310548
4	11.181300933725034	18.889293996282515	41.450441675030106	28.47896339496235
5	11.348051429117824	39.43839676978248	36.57425294611977	12.639298854979932
6	26.81565635560379	40.73889515552885	19.62571704656498	12.819731442302384
7	24.126114682361685	34.74644984697047	24.546761270040182	16.580674200627666
8	23.90743442896312	36.31577024347127	22.077610111687846	17.69918521587776
9	25.897323673983735	15.9111068268197	21.372671420675353	36.818898078521215
10-11	22.38223100501962	27.26413356209376	30.993436482831694	19.360198950054922
12-13	24.171009046505898	25.218000035760014	30.471223684323046	20.139767233411046
14-15	21.363070634576978	26.26347511141965	28.525684629335807	23.847769624667567
16-17	20.093978868941882	29.698379838193716	28.868125623370876	21.339515669493522
18-19	19.941959944121095	28.235756436996688	31.605476879208116	20.2168067396741
20-21	21.051880782335797	27.303625054708935	31.556112513439157	20.088381649516112
22-23	20.618329494314548	27.46264050381194	31.218607955980982	20.700422045892537
24-25	20.34185017642902	27.587606201408164	31.465390915246438	20.605152706916375
26-27	20.19060864319514	27.968372600678045	31.330047039964924	20.510971716161894
28-29	20.16755898264318	27.632539435131726	31.48898474990924	20.710916832315856
30-31	20.392497238316388	27.57279689167748	31.51405562858718	20.52065024141896
32-33	20.20149212541192	27.820629329585266	31.39293801934617	20.58494052565664
34-35	20.361868009792026	27.91259475431705	31.337160172985172	20.38837706290575
36-37	20.415935594661807	27.705458765984144	31.185452204799148	20.6931534345549
38-39	20.354482789716354	27.943029634944683	31.12112305098212	20.581364524356843
40-41	20.354405050557663	27.763024612995036	31.249625880298797	20.632944456148504
42-43	20.408519437712446	27.69742352893161	30.972731036010735	20.921325997345207
44-45	20.415974464241156	28.05260297912004	30.70607368272939	20.825348873909416
46-47	20.520082249180813	27.906999343094917	30.352124446586622	21.22079396113765
48-49	20.608257809329682	28.055282171359806	30.190049955455216	21.146410063855292
50-51	20.61528403256037	28.329364736941532	29.853523421715256	21.20182780878284
52-53	20.506151888323036	28.374093269887812	29.830186581754774	21.289568260034375
54-55	20.573823825963792	28.321386120295127	29.78078334640647	21.32400670733461
56-57	20.638580545153623	28.41576145894634	29.445844181185105	21.499813814714937
58-59	20.399027327646454	28.379262923940786	29.743274202337926	21.478435546074834
60-61	20.75651084888829	28.521642193083856	29.538431519186414	21.18341543884144
62-63	20.380836364512696	28.57722569154812	29.64396242710982	21.397975516829362
64-65	20.402486720208216	28.399747192255937	29.589778233502003	21.607987854033848
66-67	20.353822006867478	28.611197703896206	29.34035214284104	21.694628146395274
68-69	20.46469864555213	28.50773623152915	29.335719991370716	21.691845131548003
70-71	20.490565187005434	28.218576082925917	29.288966558946107	22.001892171122545
72-73	20.35459939845439	28.325506295705765	29.104180578736944	22.215713727102905
74-75	20.35472391898799	28.28606466734429	29.02575304980555	22.33345836386216
76-77	20.280164154007494	28.053691327341717	29.34773736291671	22.31840715573408
78-79	20.172573158378764	28.13003118117655	29.12777441339974	22.569621247044942
80-81	20.228273265581066	28.229926000094842	28.825096999035253	22.716703735288835
82-83	20.335708782892407	27.999312785837173	28.878464931476817	22.786513499793603
84-85	20.400815328296353	27.799289930524512	28.822687085115827	22.977207656063303
86-87	20.162957014470773	28.014077023270858	28.94729784199731	22.875668120261064
88-89	20.244195004621606	27.78783207356635	28.874900979438706	23.093071942373335
90-91	20.349834259984824	27.71305987785614	28.773073000236327	23.16403286192271
92-93	20.32428112656479	27.619129430257477	28.688470427635338	23.36811901554239
94-95	20.16958020076915	27.351279158986685	28.88810458715454	23.591036053089624
96-97	20.19301855711457	27.33207758678994	28.921104860018982	23.553798996076505
98-99	20.182614678364164	27.216817031598012	28.805036091470598	23.795532198567226
100-101	20.099327631925707	27.347010803444476	28.72680742162508	23.826854143004734
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	94.0
1	84.5
2	138.5
3	204.5
4	182.0
5	181.5
6	294.0
7	638.0
8	1240.5
9	1605.0
10	1402.5
11	1205.0
12	1251.0
13	1344.0
14	1436.0
15	1548.5
16	1708.5
17	1850.5
18	2024.0
19	2262.0
20	2557.5
21	2954.0
22	3463.0
23	4096.0
24	5087.0
25	6480.5
26	8435.5
27	11104.0
28	14118.0
29	18025.0
30	23114.0
31	27342.5
32	30982.5
33	34418.5
34	36913.5
35	39768.5
36	43314.0
37	45127.5
38	45295.0
39	46250.5
40	46777.0
41	46681.0
42	46413.5
43	47386.5
44	48930.5
45	49545.5
46	49492.5
47	49597.5
48	49354.0
49	48391.5
50	46956.0
51	44577.0
52	41083.5
53	36903.5
54	33026.0
55	28600.5
56	23652.5
57	18875.5
58	15254.5
59	12162.5
60	9065.5
61	6740.5
62	5090.5
63	3539.5
64	2344.0
65	1625.0
66	1153.5
67	868.0
68	682.0
69	499.0
70	365.5
71	277.0
72	230.5
73	209.5
74	166.5
75	116.5
76	83.5
77	53.5
78	26.0
79	19.5
80	12.5
81	6.5
82	4.5
83	5.0
84	5.0
85	3.5
86	1.5
87	1.5
88	1.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14319553030933188
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	3.886957934563841E-5
44-45	0.0
46-47	0.0013993048564429826
48-49	5.441741108389378E-4
50-51	3.1095663476510727E-4
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.002759740133540327
70-71	0.0
72-73	0.0
74-75	3.886957934563841E-5
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	1.1660873803691521E-4
88-89	3.1095663476510727E-4
90-91	7.773915869127682E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.002954088030268519
100-101	3.1095663476510727E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1286353.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.638801263913734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.59478639606974	36.107473288433276
2	15.990621528596646	16.83454297460519
3	6.4714653620207825	10.219505372331405
4	3.162919581863579	6.659691811338324
5	1.7937747792022223	4.721107705732327
6	1.109965710987885	3.505635868227
7	0.6962841552558908	2.565609429020882
8	0.4872799466006202	2.0519865815200435
9	0.3361433078757983	1.5924762701521842
>10	1.297119944611069	10.871481464086676
>50	0.039641485817112236	1.4521659429497387
>100	0.0188127543538374	1.8511958078861197
>500	5.925233724403034E-4	0.19649523629048712
>1k	2.962616862201517E-4	0.440086949850599
>5k	2.962616862201517E-4	0.9305452975756641
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6761	0.5255944519117225	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5173	0.40214466790997494	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3004	0.23352843270859555	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT	2640	0.2052313789449708	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	1.5547831738255363E-4
9	0.0	0.0	0.0	0.0	2.3321747607383042E-4
10-11	0.0	0.0	0.0	7.773915869127682E-5	2.3321747607383042E-4
12-13	0.0	0.0	0.0	3.1095663476510727E-4	2.3321747607383042E-4
14-15	0.0	0.0	0.0	4.2756537280202246E-4	2.3321747607383042E-4
16-17	0.0	0.0	0.0	7.773915869127682E-4	2.3321747607383042E-4
18-19	0.0	0.0	0.0	0.001166087380369152	2.3321747607383042E-4
20-21	0.0	0.0	0.0	0.0016713919118624514	2.3321747607383042E-4
22-23	0.0	0.0	0.0	0.0029152184509228804	3.1095663476510727E-4
24-25	0.0	0.0	0.0	0.004858697418204801	4.6643495214766085E-4
26-27	0.0	0.0	0.0	0.006918785123523636	4.6643495214766085E-4
28-29	0.0	0.0	0.0	0.012749222025369397	4.6643495214766085E-4
30-31	0.0	0.0	0.0	0.03031827188959796	4.6643495214766085E-4
32-33	0.0	0.0	0.0	0.06716663310926317	4.6643495214766085E-4
34-35	0.0	0.0	0.0	0.12170065293119385	5.441741108389377E-4
36-37	0.0	0.0	0.0	0.21284981649671592	5.441741108389377E-4
38-39	0.0	0.0	0.0	0.35581212932997397	5.441741108389377E-4
40-41	0.0	0.0	0.0	0.5084140978409504	5.441741108389377E-4
42-43	0.0	0.0	0.0	0.6535919766969098	5.441741108389377E-4
44-45	0.0	0.0	0.0	0.825240039087249	5.441741108389377E-4
46-47	0.0	0.0	0.0	1.0434538575336632	5.441741108389377E-4
48-49	0.0	0.0	0.0	1.2766713336074935	6.219132695302145E-4
50-51	0.0	0.0	0.0	1.5125708106561728	6.219132695302145E-4
52-53	0.0	0.0	0.0	1.738092110019567	6.607828488758529E-4
54-55	0.0	0.0	0.0	1.9671894106827597	6.996524282214913E-4
56-57	0.0	0.0	0.0	2.2336792466764566	6.996524282214913E-4
58-59	0.0	0.0	0.0	2.5405934451896175	6.996524282214913E-4
60-61	0.0	0.0	0.0	2.8599459090933825	6.996524282214913E-4
62-63	0.0	0.0	0.0	3.17669411118099	6.996524282214913E-4
64-65	0.0	0.0	0.0	3.4831030051626577	6.996524282214913E-4
66-67	0.0	0.0	0.0	3.821967997897933	6.996524282214913E-4
68-69	0.0	0.0	0.0	4.215561358351868	6.996524282214913E-4
70-71	0.0	0.0	0.0	4.61961063564978	7.773915869127682E-4
72-73	0.0	0.0	0.0	5.01724643235566	9.328699042953217E-4
74-75	0.0	0.0	0.0	5.409984662063991	9.328699042953217E-4
76-77	0.0	0.0	0.0	5.820175332898512	9.328699042953217E-4
78-79	0.0	0.0	0.0	6.280274543612833	9.328699042953217E-4
80-81	0.0	0.0	0.0	6.764356284783415	9.717394836409601E-4
82-83	0.0	0.0	0.0	7.260837421765254	0.0010106090629865986
84-85	0.0	0.0	0.0	7.759378646452412	0.0010106090629865986
86-87	0.0	0.0	0.0	8.270008310316065	0.0010106090629865986
88-89	0.0	0.0	0.0	8.825571207903273	0.0010106090629865986
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2465	0.0	65.6321	1
GTATCAA	4865	0.0	46.067253	1
TACACCG	165	0.0	37.42282	5
TCAACGC	5960	0.0	37.297237	4
ATCAACG	5975	0.0	37.203606	3
CAACGCA	6005	0.0	37.017742	5
AACGCAG	6080	0.0	36.56111	6
TATCAAC	6235	0.0	35.880753	2
ACGCAGA	6925	0.0	32.031273	7
CGCAGAG	7015	0.0	31.620323	8
GTACATG	5055	0.0	28.33346	1
TACATGG	5015	0.0	27.655975	2
GCAGAGT	8005	0.0	27.353737	9
ACATGGG	4920	0.0	26.452246	3
TCGATCG	55	9.482305E-5	25.908607	40-41
GAGTACT	5245	0.0	24.99428	12-13
CAGAGTA	7615	0.0	24.29487	10-11
CATGGGA	2925	0.0	23.870888	4
ATGGGAT	1475	0.0	23.829601	5
GTACTTT	5800	0.0	22.684483	14-15
>>END_MODULE