FastQCFastQC Report
Fri 10 Feb 2017
SRR2031524_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031524_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979599
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.14947471684922048No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20420.10315220405748841No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21750.050.8850561
GTATCAA41500.043.265061
TCAACGC47850.036.7293624
ATCAACG48500.036.1391753
CAACGCA48650.036.125395
AACGCAG49800.035.195786
TATCAAC51150.034.3597262
ACGCAGA57800.030.3243947
GTACATG61250.029.779591
CGCAGAG59000.029.46618
TACATGG62650.027.6735842
ACATGGG62050.027.0225623
GCAGAGT69950.024.4460339
ACGACCG1050.002196778322.6190475
CATGGGG44800.022.477684
AGAGTAC61750.020.19230810-11
CAGAGTA67350.020.1707510-11
CATGGGA22250.019.2134824
GTCTACG1759.941387E-519.01
GAGTACT39000.018.14743612-13
Produced by FastQC (version 0.11.5)