FastQCFastQC Report
Fri 10 Feb 2017
SRR2031524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979599
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40130.2027178231550935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28400.14346339839533157No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.047.6574861
GTATCAA42950.042.0767361
TCAACGC50050.035.683514
CAACGCA50950.035.0531855
ATCAACG51150.034.9161223
AACGCAG53650.033.466166
TATCAAC53450.033.413652
GTACATG54500.028.8837181
ACGCAGA62850.028.4917987
CGCAGAG63900.027.9492918
TACATGG55250.027.424722
ACATGGG55800.027.0692793
CATGGGG35750.024.181274
GCAGAGT75150.023.5124449
GAGTACT46050.020.4229912-13
AGAGTAC65750.019.8303510-11
CAGAGTA72800.019.57373410-11
CATGGGA21750.018.999574
ACGTAAC1801.2224456E-418.4946961
GTACTTT52050.017.88624214-15
Produced by FastQC (version 0.11.5)