FastQCFastQC Report
Fri 10 Feb 2017
SRR2031523_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031523_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874366
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23500.12537572704583844No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20450.049.7066041
GTATCAA39600.041.2626231
TCAACGC42300.037.842794
ATCAACG42950.037.159493
AACGCAG44000.036.4886326
CAACGCA44150.036.472255
TATCAAC47450.033.8356172
ACGCAGA49550.032.4016157
CGCAGAG50650.031.5103658
GCAGAGT60900.025.5829229
GTACATG50850.023.4464111
ACATGGG48750.023.287183
TACATGG54450.021.8092
ACCGCTC1553.908113E-521.4516128
AGAGTAC52700.021.0009510-11
GAGTACT32600.020.90874312-13
CAGAGTA58850.020.58198710-11
TATACCG1656.325441E-520.1515165
CATGGGG26000.019.7307684
GTATATA15950.017.868341
Produced by FastQC (version 0.11.5)