##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031523_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1874366 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.29047528604339 34.0 31.0 34.0 30.0 34.0 2 32.45905122051936 34.0 31.0 34.0 31.0 34.0 3 32.5617088658245 34.0 31.0 34.0 31.0 34.0 4 35.78173313002903 37.0 35.0 37.0 35.0 37.0 5 35.76998409062051 37.0 37.0 37.0 35.0 37.0 6 35.89071824819699 37.0 37.0 37.0 35.0 37.0 7 35.91237250355587 37.0 37.0 37.0 35.0 37.0 8 35.95734290954915 37.0 37.0 37.0 35.0 37.0 9 37.7903957924973 39.0 39.0 39.0 35.0 39.0 10-11 37.795394015896576 39.0 39.0 39.0 35.0 39.0 12-13 37.77453282870048 39.0 39.0 39.0 35.0 39.0 14-15 39.32017199415696 41.0 40.0 41.0 37.0 41.0 16-17 39.33625983399187 41.0 40.0 41.0 37.0 41.0 18-19 39.29302308087108 41.0 40.0 41.0 36.5 41.0 20-21 39.26036030316384 41.0 40.0 41.0 36.5 41.0 22-23 39.19731258462862 41.0 40.0 41.0 36.0 41.0 24-25 39.15831139702705 41.0 39.5 41.0 36.0 41.0 26-27 39.072755000890965 41.0 39.0 41.0 36.0 41.0 28-29 38.98059663907689 41.0 39.0 41.0 36.0 41.0 30-31 38.84208687097397 41.0 39.0 41.0 35.0 41.0 32-33 38.76272857062068 41.0 39.0 41.0 35.0 41.0 34-35 38.65875047882858 40.0 39.0 41.0 35.0 41.0 36-37 38.543226883116745 40.0 38.0 41.0 35.0 41.0 38-39 38.4177647268463 40.0 38.0 41.0 34.0 41.0 40-41 38.31440951233644 40.0 38.0 41.0 34.0 41.0 42-43 38.19221619470264 40.0 38.0 41.0 34.0 41.0 44-45 38.364385344164376 40.0 38.0 41.0 34.0 41.0 46-47 38.27692136967914 40.0 38.0 41.0 34.0 41.0 48-49 38.18961398147427 40.0 38.0 41.0 34.0 41.0 50-51 37.176712285647525 39.0 37.0 40.0 32.0 40.5 52-53 37.265334785202036 39.5 37.5 40.0 33.0 40.5 54-55 38.07384043457895 40.0 38.0 41.0 33.5 41.0 56-57 38.13907982752568 41.0 38.0 41.0 34.0 41.0 58-59 37.98418131784294 40.0 38.0 41.0 33.0 41.0 60-61 37.858894420833494 40.0 37.5 41.0 33.0 41.0 62-63 37.64486685097788 40.0 37.0 41.0 33.0 41.0 64-65 37.4150614127657 40.0 36.5 41.0 32.5 41.0 66-67 37.1727093321155 39.5 36.0 41.0 32.0 41.0 68-69 36.91399945368194 39.0 35.5 41.0 32.0 41.0 70-71 36.59077869530284 39.0 35.0 41.0 31.5 41.0 72-73 36.2502467501011 37.5 35.0 40.5 31.0 41.0 74-75 35.86755788357237 37.0 35.0 39.5 31.0 41.0 76-77 35.48431309573477 37.0 35.0 39.0 31.0 41.0 78-79 35.052149366772554 36.0 35.0 39.0 30.0 40.5 80-81 34.65631525539836 36.0 35.0 38.0 30.0 39.5 82-83 34.29535346885294 35.0 35.0 37.0 29.5 39.0 84-85 33.94143673114002 35.0 34.0 37.0 29.0 39.0 86-87 33.622066874879295 35.0 34.0 36.0 29.0 37.0 88-89 33.3576990833167 35.0 34.0 36.0 29.0 37.0 90-91 33.12591270861721 35.0 34.0 36.0 29.0 37.0 92-93 32.931594203053194 35.0 34.0 35.0 28.0 36.0 94-95 32.76872953307945 35.0 34.0 35.0 27.0 36.0 96-97 32.62471737110042 35.0 34.0 35.0 27.0 36.0 98-99 32.484783654846495 35.0 34.0 35.0 26.5 36.0 100-101 30.94001838488321 34.0 31.5 35.0 22.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2793708865402209 1101 2 0.2537628608386697 1101 3 0.3499690356281988 1101 4 0.24600399601529688 1101 5 0.16183118251322526 1101 6 0.181853600790852 1101 7 0.16981561754009533 1101 8 0.25585043069369817 1101 9 0.446944748321215 1101 10-11 0.4842016363998951 1101 12-13 0.5297669899437594 1101 14-15 0.6629444107704927 1101 16-17 0.411603922909471 1101 18-19 0.5729705816700772 1101 20-21 0.5458906534729024 1101 22-23 0.6097428654895793 1101 24-25 0.6186337653542395 1101 26-27 0.49086366106043045 1101 28-29 0.4889845475504515 1101 30-31 0.5818571570224762 1101 32-33 0.6021508031493568 1101 34-35 0.6518238368391067 1101 36-37 0.4859301645369456 1101 38-39 0.5373252279402223 1101 40-41 0.548929734076772 1101 42-43 0.6166978438309769 1101 44-45 0.7030140769393469 1101 46-47 0.6582790835454091 1101 48-49 0.6060327164504287 1101 50-51 0.7047884541142082 1101 52-53 0.7243741431539661 1101 54-55 0.7768818684734029 1101 56-57 0.652609572914379 1101 58-59 0.6202538031061096 1101 60-61 0.5809151021023453 1101 62-63 0.7189072876068536 1101 64-65 0.70255774231142 1101 66-67 0.6727724426939901 1101 68-69 0.700705097331749 1101 70-71 0.6535357546340919 1101 72-73 0.5696550275319581 1101 74-75 0.6276624666550603 1101 76-77 0.7790394615420624 1101 78-79 0.7430035037545153 1101 80-81 0.7250594942816093 1101 82-83 0.6717077805249261 1101 84-85 0.6275448040056304 1101 86-87 0.5384386752453807 1101 88-89 0.5766516399488637 1101 90-91 0.5426136076486898 1101 92-93 0.5598884890044857 1101 94-95 0.5866113077649118 1101 96-97 0.6701062210180311 1101 98-99 0.5817577968303596 1101 100-101 0.5129938627133477 1102 1 0.11873727937231848 1102 2 0.25100747974467197 1102 3 0.3135167790754707 1102 4 0.1289671724238417 1102 5 0.07042951558377553 1102 6 0.11515032619331578 1102 7 0.15029905970047253 1102 8 0.22663621449305538 1102 9 0.36664724545328653 1102 10-11 0.380406463209944 1102 12-13 0.4098258163514785 1102 14-15 0.37184530295289164 1102 16-17 0.44257722267948907 1102 18-19 0.45992384131635333 1102 20-21 0.46176426912224855 1102 22-23 0.4982801430556236 1102 24-25 0.46955340369687093 1102 26-27 0.4877505371172077 1102 28-29 0.4756917910276286 1102 30-31 0.4937050782538108 1102 32-33 0.5003610015389768 1102 34-35 0.49123780185859545 1102 36-37 0.40941206807685404 1102 38-39 0.4127301352152344 1102 40-41 0.42002079539867765 1102 42-43 0.4238385650796985 1102 44-45 0.40621663659487695 1102 46-47 0.5262132545117026 1102 48-49 0.5746897128240676 1102 50-51 0.49027404381904205 1102 52-53 0.4840704423096298 1102 54-55 0.49962273325421336 1102 56-57 0.4180300294986381 1102 58-59 0.445482557367221 1102 60-61 0.31953501935392126 1102 62-63 0.4358418989556725 1102 64-65 0.38434167215467596 1102 66-67 0.3624098610737079 1102 68-69 0.18349755754780972 1102 70-71 0.15154397510807627 1102 72-73 0.12952693601938137 1102 74-75 0.05034515744671353 1102 76-77 0.1021043608793164 1102 78-79 0.19198409973888175 1102 80-81 0.08776724882000053 1102 82-83 0.18711872267170548 1102 84-85 0.262251840883593 1102 86-87 0.25955963908507584 1102 88-89 0.1804786185441003 1102 90-91 0.11258604296411079 1102 92-93 0.08477037405921095 1102 94-95 0.1672665534820652 1102 96-97 0.21384680852095528 1102 98-99 0.22634625663322083 1102 100-101 0.01685482144243977 1103 1 0.14831343892529958 1103 2 0.1702066314813635 1103 3 0.2886067528841352 1103 4 0.16322745153535578 1103 5 0.06147924142274519 1103 6 0.09851395056615075 1103 7 0.17345414696778505 1103 8 0.17462784149016386 1103 9 0.19179828668278986 1103 10-11 0.27286913328656937 1103 12-13 0.37056237680823045 1103 14-15 0.4542379296399517 1103 16-17 0.37296079117520975 1103 18-19 0.4561017744796487 1103 20-21 0.48079238069149 1103 22-23 0.42082900983957217 1103 24-25 0.44844317571755 1103 26-27 0.5625161598421684 1103 28-29 0.40692542144724797 1103 30-31 0.46324759505377955 1103 32-33 0.60008247243303 1103 34-35 0.524637741219415 1103 36-37 0.5793867674932471 1103 38-39 0.6289327865519496 1103 40-41 0.590031038966039 1103 42-43 0.4637550748667678 1103 44-45 0.6403956352262128 1103 46-47 0.5940092259461949 1103 48-49 0.6121348335114547 1103 50-51 0.5833877285741167 1103 52-53 0.6031737533115304 1103 54-55 0.6389913492249306 1103 56-57 0.5688584223377404 1103 58-59 0.785928717828078 1103 60-61 0.6944512978898842 1103 62-63 0.6417238992263208 1103 64-65 0.7696458358736109 1103 66-67 0.7181755506045349 1103 68-69 0.7406504111007735 1103 70-71 0.6362689832875077 1103 72-73 0.6995591912657844 1103 74-75 0.7369022738996307 1103 76-77 0.5728750779804201 1103 78-79 0.6527874660697464 1103 80-81 0.6781457469211745 1103 82-83 0.6554887107322998 1103 84-85 0.7299028093118238 1103 86-87 0.7605270395314747 1103 88-89 0.6947549481951185 1103 90-91 0.6269116205351537 1103 92-93 0.5353040502151671 1103 94-95 0.6174332255731372 1103 96-97 0.6030072984992216 1103 98-99 0.7045825301673077 1103 100-101 0.7414767258894877 1104 1 0.22894722430962133 1104 2 0.13620112460920097 1104 3 0.20406484010293724 1104 4 0.03558226776349471 1104 5 0.05950194679746801 1104 6 0.11072427804605667 1104 7 0.1294631499040193 1104 8 0.13529102732615428 1104 9 0.3304926324275357 1104 10-11 0.309055681437421 1104 12-13 0.3639790748937699 1104 14-15 0.40341170839774065 1104 16-17 0.3922230378724194 1104 18-19 0.4634989205031559 1104 20-21 0.45328086995765915 1104 22-23 0.42938846387470875 1104 24-25 0.4403487210540291 1104 26-27 0.4604240435047302 1104 28-29 0.5142543980297134 1104 30-31 0.37813062162462785 1104 32-33 0.41540460160459247 1104 34-35 0.4113142334256352 1104 36-37 0.5137226486118394 1104 38-39 0.49179453207047885 1104 40-41 0.5334808696849436 1104 42-43 0.418479521799334 1104 44-45 0.5508933483128473 1104 46-47 0.5461687500549459 1104 48-49 0.5406170923192093 1104 50-51 0.33330013655488955 1104 52-53 0.45125282224645247 1104 54-55 0.5382433895477305 1104 56-57 0.4883712583769011 1104 58-59 0.609497254774773 1104 60-61 0.5097688570430563 1104 62-63 0.598738306209313 1104 64-65 0.5553662421482457 1104 66-67 0.6629756313415314 1104 68-69 0.634089197608553 1104 70-71 0.5167671914594578 1104 72-73 0.546299816063474 1104 74-75 0.5544033497581253 1104 76-77 0.43911730418871286 1104 78-79 0.525710107083377 1104 80-81 0.561742237147044 1104 82-83 0.5100126481078107 1104 84-85 0.52963108363479 1104 86-87 0.5053857688378613 1104 88-89 0.5497705433828912 1104 90-91 0.4578872052118754 1104 92-93 0.44208500542517726 1104 94-95 0.45967219183496866 1104 96-97 0.446610796086631 1104 98-99 0.44296097339053375 1104 100-101 0.5584629130088885 1105 1 0.36671364529011186 1105 2 0.3199675849567427 1105 3 0.35086778833706234 1105 4 0.30870298324172296 1105 5 0.26097105350543615 1105 6 0.29680051054784684 1105 7 0.24519798061676568 1105 8 0.21364004484986054 1105 9 0.3849909441179378 1105 10-11 0.29447877880108564 1105 12-13 0.326562841711997 1105 14-15 0.36101160675917754 1105 16-17 0.38443874067267103 1105 18-19 0.43834015498225654 1105 20-21 0.39779211909202417 1105 22-23 0.421705805238922 1105 24-25 0.3650379450400152 1105 26-27 0.41449003341602975 1105 28-29 0.37756233961616914 1105 30-31 0.41139989403207977 1105 32-33 0.27991789066788897 1105 34-35 0.3059023377769492 1105 36-37 0.34065740488506435 1105 38-39 0.2861074249581108 1105 40-41 0.22622015424250463 1105 42-43 0.3709375466828888 1105 44-45 0.39215341598838194 1105 46-47 0.3786064830139324 1105 48-49 0.36446135627169696 1105 50-51 0.40106693331176047 1105 52-53 0.26482819135727453 1105 54-55 0.26353564515440553 1105 56-57 0.4089960134512296 1105 58-59 0.32171410279827484 1105 60-61 0.37366587284177655 1105 62-63 0.350638392637876 1105 64-65 0.3831371903394185 1105 66-67 0.257955974657996 1105 68-69 0.2862519696214463 1105 70-71 0.23677746268054278 1105 72-73 0.11926127393267194 1105 74-75 0.22664761156332247 1105 76-77 0.16310776304745644 1105 78-79 0.2680300430940221 1105 80-81 0.39280176549400636 1105 82-83 0.308837183800307 1105 84-85 0.2525262438139464 1105 86-87 0.21084955741533662 1105 88-89 0.24608364026340723 1105 90-91 0.23083291626131341 1105 92-93 0.14477413278294904 1105 94-95 0.23116686923094676 1105 96-97 0.21223727794289005 1105 98-99 0.17653874031255157 1105 100-101 0.21627664602513974 1106 1 0.09326070588324598 1106 2 0.14637579936169942 1106 3 0.20857424766008137 1106 4 0.19845481220966832 1106 5 0.11964811380104834 1106 6 0.1893590160339258 1106 7 0.11213043855794069 1106 8 0.11281655471950813 1106 9 0.17579403708207053 1106 10-11 0.19318982335418156 1106 12-13 0.19046795687169293 1106 14-15 0.19691194164296633 1106 16-17 0.18475659926774313 1106 18-19 0.24252240052182117 1106 20-21 0.2462524140410025 1106 22-23 0.18609555971257663 1106 24-25 0.20186326000598598 1106 26-27 0.23634277050416586 1106 28-29 0.2247452496339264 1106 30-31 0.2638171400509961 1106 32-33 0.3191546663949367 1106 34-35 0.2531480591655395 1106 36-37 0.3742109086025067 1106 38-39 0.40855968005161003 1106 40-41 0.4683017891894323 1106 42-43 0.3316941592325904 1106 44-45 0.3717195547089389 1106 46-47 0.39526686854351567 1106 48-49 0.44820015353312215 1106 50-51 0.3168382073577689 1106 52-53 0.3442787902141049 1106 54-55 0.32834342583031884 1106 56-57 0.3521171765855797 1106 58-59 0.37451565523089414 1106 60-61 0.36202874404870755 1106 62-63 0.33353849280548786 1106 64-65 0.2592536624561248 1106 66-67 0.3324205077215652 1106 68-69 0.40586911778684964 1106 70-71 0.45900012568431237 1106 72-73 0.38837725347600127 1106 74-75 0.39501134229428203 1106 76-77 0.3932664774639889 1106 78-79 0.35629373845226553 1106 80-81 0.39489156591913854 1106 82-83 0.39813528326045855 1106 84-85 0.43002241727911894 1106 86-87 0.3292182469387299 1106 88-89 0.3325570462595806 1106 90-91 0.3097246592107439 1106 92-93 0.2408513973808013 1106 94-95 0.17156251475002193 1106 96-97 0.19908837862951856 1106 98-99 0.12067050534512447 1106 100-101 0.07505701449647617 1107 1 0.20785958836353302 1107 2 0.20010961349561995 1107 3 0.22852933566709055 1107 4 0.1547333286851611 1107 5 0.21596725606273282 1107 6 0.1297040246218657 1107 7 0.11766213008770876 1107 8 0.21107753501848947 1107 9 0.22108681917193707 1107 10-11 0.22898648321882575 1107 12-13 0.26602089307864674 1107 14-15 0.34024984071832165 1107 16-17 0.19596270058994492 1107 18-19 0.23991806399867954 1107 20-21 0.319098362177904 1107 22-23 0.2728957510389094 1107 24-25 0.4030329596298543 1107 26-27 0.28096528924078257 1107 28-29 0.3080998587039119 1107 30-31 0.26771743294179373 1107 32-33 0.2888752554067082 1107 34-35 0.2620459760069096 1107 36-37 0.3336310904246389 1107 38-39 0.3041316867728838 1107 40-41 0.45918272892473766 1107 42-43 0.4164301382115454 1107 44-45 0.24302650612882104 1107 46-47 0.41062531035204586 1107 48-49 0.35955363203841983 1107 50-51 0.41306386061606304 1107 52-53 0.33718403087840443 1107 54-55 0.34127224063202277 1107 56-57 0.34119340584173585 1107 58-59 0.33878763679399526 1107 60-61 0.3467122325240908 1107 62-63 0.42447782434358317 1107 64-65 0.4567713896483738 1107 66-67 0.44303104371483215 1107 68-69 0.4045340264875463 1107 70-71 0.4349249990393247 1107 72-73 0.26512489217982704 1107 74-75 0.3403285060677561 1107 76-77 0.36170726103228645 1107 78-79 0.20708358076333866 1107 80-81 0.3852393698593488 1107 82-83 0.37727092928189165 1107 84-85 0.3085244501214035 1107 86-87 0.3121426823552227 1107 88-89 0.35277955629955215 1107 90-91 0.4096532224307339 1107 92-93 0.4686797503281994 1107 94-95 0.36233723109941707 1107 96-97 0.3532705362066295 1107 98-99 0.3297931374488243 1107 100-101 0.3495604512267825 1108 1 0.07371936542229918 1108 2 0.07974073740142984 1108 3 0.07883963689462092 1108 4 -0.040741946652445904 1108 5 0.15074293093019264 1108 6 0.0978013856043134 1108 7 0.10028619859092913 1108 8 0.11957755390982783 1108 9 0.22099719514473293 1108 10-11 0.17431157973306455 1108 12-13 0.19411679547398109 1108 14-15 0.24883245430140022 1108 16-17 0.1865079514976813 1108 18-19 0.1952869093395364 1108 20-21 0.250060329665466 1108 22-23 0.2348401003757985 1108 24-25 0.15618422536422116 1108 26-27 0.19305305166565034 1108 28-29 0.17421474206233967 1108 30-31 0.27304947678258884 1108 32-33 0.14297934756364583 1108 34-35 0.19260668559490313 1108 36-37 0.24574199107875216 1108 38-39 0.2265591623364216 1108 40-41 0.3346063562200001 1108 42-43 0.20156268365401075 1108 44-45 0.15665193568712255 1108 46-47 0.2104110913536843 1108 48-49 0.1969466506781643 1108 50-51 0.07161066852160758 1108 52-53 0.08579668901282389 1108 54-55 0.19093760033044305 1108 56-57 0.18936151708565774 1108 58-59 0.2801852385604704 1108 60-61 0.24828777020235293 1108 62-63 0.28411414995763096 1108 64-65 0.28812591623288597 1108 66-67 0.24295360386910403 1108 68-69 0.12725090565004393 1108 70-71 0.13253133457938304 1108 72-73 0.10877914494242447 1108 74-75 0.12095173280805227 1108 76-77 0.14293252106644871 1108 78-79 0.12706645847369913 1108 80-81 0.23145625473318177 1108 82-83 0.15187396952806154 1108 84-85 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-0.5434093907381552 2316 50-51 -0.5812224731092144 2316 52-53 -0.6511167468500858 2316 54-55 -0.6406581542717618 2316 56-57 -0.6112613999584298 2316 58-59 -0.5747335120894093 2316 60-61 -0.571720076558556 2316 62-63 -0.5767162560264936 2316 64-65 -0.8856394248814539 2316 66-67 -0.630459597770141 2316 68-69 -0.4923274059885614 2316 70-71 -0.8668919499332404 2316 72-73 -0.5702945513643414 2316 74-75 -0.38467638861092723 2316 76-77 -0.5072052796322524 2316 78-79 -0.4986193559332577 2316 80-81 -0.43243567435868613 2316 82-83 -0.4198613973884022 2316 84-85 -0.3839876317154065 2316 86-87 -0.3464334030743359 2316 88-89 -0.32722537709409494 2316 90-91 -0.3489778382599624 2316 92-93 -0.2774573250100403 2316 94-95 -0.2269244522497189 2316 96-97 -0.3427996728431566 2316 98-99 -0.263152773309848 2316 100-101 -0.27600813345337016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 47.0 7 914.0 8 1766.0 9 1344.0 10 1482.0 11 1845.0 12 2327.0 13 2545.0 14 2666.0 15 2733.0 16 3010.0 17 3493.0 18 4107.0 19 5112.0 20 6312.0 21 7875.0 22 8698.0 23 8715.0 24 8579.0 25 8698.0 26 9136.0 27 10070.0 28 11880.0 29 14051.0 30 17048.0 31 21337.0 32 27757.0 33 37104.0 34 54667.0 35 86888.0 36 160240.0 37 353697.0 38 657774.0 39 330219.0 40 230.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.541921908528 22.04873541240078 14.592294141058899 22.81704853801232 2 13.424539284216635 22.26480847390531 43.07899311020366 21.23165913167439 3 16.295643433566337 28.94968218586978 32.37286634520686 22.38180803535702 4 10.997905425087737 19.16973525981585 42.878605352423165 26.953753962673243 5 11.36437600767406 39.77350208017004 37.344894220232334 11.517227691923562 6 26.182453160161888 41.659046312193034 20.410421443837542 11.748079083807538 7 23.50874909169287 34.590522875468295 26.21521090331344 15.685517129525397 8 22.775061007295268 38.025497688285 22.980143685918332 16.2192976185014 9 24.91221031538131 17.386465610238343 22.631759218850533 35.06956485552982 10-11 21.9508356425586 27.943075151811332 31.992284324406228 18.11380488122384 12-13 23.13424912743829 25.896329745631324 31.214981492408633 19.754439634521752 14-15 20.612142985948314 27.5023127820287 28.479603236507707 23.405940995515284 16-17 20.12955313956826 30.681547787358497 28.94626769798428 20.242631375088962 18-19 20.07097333178259 29.927879613693374 30.477745541692496 19.52340151283154 20-21 20.936972821743456 29.163167705773578 30.206213727735136 19.693645744747823 22-23 20.78350759670203 28.921806093367035 29.823711057498908 20.470975252432023 24-25 20.44598013408267 29.19397812380293 29.834568061947348 20.525473680167053 26-27 20.442939105809643 29.405623021330946 29.879249837011557 20.272188035847854 28-29 20.3616849644093 29.237539520029703 30.143605891272035 20.25716962428896 30-31 20.80252736125175 29.223988271234113 29.785458122906626 20.18802624460751 32-33 20.55428342170099 29.193604664190453 29.827872464609367 20.42423944949919 34-35 20.645594296951607 29.405516318584525 29.621802785581895 20.32708659888197 36-37 20.74424098601874 29.21193086088843 29.60779805011401 20.43603010297882 38-39 20.39852408761149 29.529291504434035 29.77025298154149 20.301931426412985 40-41 20.5814926220386 29.152390728384958 29.979843851200886 20.286272798375556 42-43 20.56199269512998 29.126568663750835 29.675954429391062 20.635484211728127 44-45 20.74365412091342 29.20534196629687 29.440754900590388 20.610249012199326 46-47 20.571293058651424 29.027290352663996 29.614823603978312 20.78659298470626 48-49 20.48237316457155 29.228967998642734 29.554012627256903 20.734646209528808 50-51 20.501758994774764 29.189549959826415 29.43766052094415 20.87103052445467 52-53 20.49943821004009 29.22886992188292 29.408103860185257 20.863588007891735 54-55 20.519525002054028 29.13537164033065 29.421628433294245 20.923474924321077 56-57 20.63943221334574 29.29006394695593 29.23679260080475 20.833711238893578 58-59 20.474016280705047 29.058919122519296 29.34442899625794 21.12263560051772 60-61 20.653597002933257 29.054651012662415 29.407730400572778 20.884021583831547 62-63 20.49877131787495 29.0050609112628 29.566610789995124 20.92955698086713 64-65 20.563433182206676 28.78933463368414 29.471165183320654 21.176067000788535 66-67 20.67811195892371 28.881552482279343 29.20486180393797 21.235473754858976 68-69 20.611422742409964 28.87925837323127 29.297639841951895 21.21167904240687 70-71 20.533636440268335 28.64723325113665 29.42781719258672 21.39131311600829 72-73 20.593763437877126 28.658143606958298 29.200753748200725 21.547339206963848 74-75 20.602993225442628 28.680391129587285 29.149483078544957 21.567132566425126 76-77 20.506987429349444 28.6484336570339 29.30068087022492 21.54389804339174 78-79 20.670135928628667 28.598256690528956 28.985961119653258 21.745646261189115 80-81 20.63580431996739 28.580464007563087 29.028802272341686 21.75492940012783 82-83 20.705694618873796 28.552161104074656 29.0846611600936 21.65748311695795 84-85 20.808609417797804 28.355587969478748 28.977051440327024 21.858751172396428 86-87 20.566527561852915 28.434628028890835 29.267389613341262 21.731454795914992 88-89 20.822774207385326 28.229358620461532 29.146121942032664 21.801745230120478 90-91 20.89749280556732 28.315867872123164 29.083247348703505 21.70339197360601 92-93 21.03834043084435 28.11740076377826 28.9900424997039 21.85421630567349 94-95 20.942974851229696 27.893858510024188 29.0929039472547 22.070262691491415 96-97 21.03193826605903 27.769096323770277 29.179946712648437 22.019018697522256 98-99 21.121141762067815 27.734818066482212 28.931436016231622 22.21260415521835 100-101 21.349752396276926 27.567134700480057 28.998685422164083 22.084427481078936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 48.0 1 36.5 2 41.0 3 59.0 4 56.5 5 69.0 6 104.0 7 199.5 8 378.5 9 507.0 10 472.5 11 422.0 12 480.0 13 565.5 14 663.5 15 736.5 16 786.0 17 899.5 18 1031.0 19 1129.0 20 1300.5 21 1606.5 22 1966.5 23 2450.5 24 3140.0 25 4233.0 26 6404.5 27 10368.0 28 16374.5 29 25112.0 30 36397.0 31 48474.5 32 60292.0 33 70463.0 34 79942.5 35 87526.0 36 93933.5 37 97330.0 38 93313.5 39 87069.0 40 80580.0 41 74315.5 42 68197.5 43 63504.5 44 60934.5 45 58210.0 46 56301.5 47 54500.5 48 52872.0 49 51550.0 50 48896.5 51 45806.5 52 43187.0 53 40953.0 54 38142.0 55 34287.5 56 30168.0 57 25890.0 58 22464.0 59 19162.0 60 15756.0 61 12965.0 62 10268.5 63 7714.5 64 5577.0 65 4037.5 66 2874.5 67 2139.0 68 1716.0 69 1301.0 70 966.0 71 742.0 72 556.0 73 387.0 74 262.5 75 177.5 76 135.0 77 100.0 78 80.0 79 57.5 80 47.0 81 43.0 82 36.5 83 34.0 84 22.5 85 18.0 86 14.5 87 10.0 88 8.0 89 8.0 90 8.5 91 6.0 92 4.0 93 4.5 94 4.0 95 2.0 96 1.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 2.1340549284398031E-4 48-49 4.2681098568796063E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1874366.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.61887697067844 #Duplication Level Percentage of deduplicated Percentage of total 1 67.13506673846119 32.64031550175531 2 15.868438868480931 15.430113541268122 3 6.679587566462571 9.742621383261515 4 3.3641875736246387 6.54252086993366 5 1.947747803571984 4.734865541588773 6 1.238782080725722 3.613691614577096 7 0.8549080530146511 2.909526861553309 8 0.5716086719714609 2.2232777358362683 9 0.41413898935707744 1.8121475315081574 >10 1.8236638792447475 14.5809420476138 >50 0.06710652539862579 2.239358817086666 >100 0.0332137216102703 2.8395154881808753 >500 0.0011068057686207417 0.3387443587510818 >1k 4.4272230744829625E-4 0.3523587070853188 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2350 0.12537572704583844 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 5.335137321099508E-5 0.0 14-15 0.0 0.0 0.0 5.335137321099508E-5 0.0 16-17 0.0 0.0 0.0 5.335137321099508E-5 0.0 18-19 0.0 0.0 0.0 1.6005411963298524E-4 0.0 20-21 0.0 0.0 0.0 2.1340549284398031E-4 0.0 22-23 0.0 0.0 0.0 5.335137321099508E-4 0.0 24-25 0.0 0.0 0.0 9.603247177979114E-4 0.0 26-27 0.0 0.0 0.0 0.0018939737489903252 0.0 28-29 0.0 0.0 0.0 0.004694920842567567 0.0 30-31 0.0 0.0 0.0 0.016779006874857953 0.0 32-33 0.0 0.0 0.0 0.04945672296659244 0.0 34-35 0.0 0.0 0.0 0.10030058163667074 0.0 36-37 0.0 0.0 0.0 0.19126467296141736 0.0 38-39 0.0 0.0 0.0 0.3221622671345938 0.0 40-41 0.0 0.0 0.0 0.46223629750006134 0.0 42-43 0.0 0.0 0.0 0.6106331420864441 0.0 44-45 0.0 0.0 0.0 0.797416299698138 0.0 46-47 0.0 0.0 0.0 1.0370439924753223 0.0 48-49 5.335137321099508E-5 0.0 0.0 1.2801128488246158 2.667568660549754E-5 50-51 8.002705981649262E-5 0.0 0.0 1.5146988368333612 5.335137321099508E-5 52-53 1.0670274642199016E-4 0.0 0.0 1.744269795760273 5.335137321099508E-5 54-55 1.0670274642199016E-4 0.0 0.0 2.0097995802313955 5.335137321099508E-5 56-57 1.0670274642199016E-4 0.0 0.0 2.3263866288654405 5.335137321099508E-5 58-59 1.0670274642199016E-4 0.0 0.0 2.6787724489240627 5.335137321099508E-5 60-61 1.0670274642199016E-4 0.0 0.0 3.0181405339192025 5.335137321099508E-5 62-63 1.0670274642199016E-4 0.0 0.0 3.354467590641316 5.335137321099508E-5 64-65 1.0670274642199016E-4 0.0 0.0 3.704345896159021 5.335137321099508E-5 66-67 1.0670274642199016E-4 0.0 0.0 4.120726688384233 5.335137321099508E-5 68-69 1.0670274642199016E-4 0.0 0.0 4.5811223635085145 5.335137321099508E-5 70-71 1.0670274642199016E-4 0.0 0.0 5.030314250258487 5.335137321099508E-5 72-73 1.0670274642199016E-4 0.0 0.0 5.473850891448095 5.335137321099508E-5 74-75 1.0670274642199016E-4 0.0 0.0 5.929338240236966 5.335137321099508E-5 76-77 1.0670274642199016E-4 0.0 0.0 6.4342556363058225 5.335137321099508E-5 78-79 1.0670274642199016E-4 0.0 0.0 6.993884865602556 5.335137321099508E-5 80-81 1.0670274642199016E-4 0.0 0.0 7.570559858640202 5.335137321099508E-5 82-83 1.0670274642199016E-4 0.0 0.0 8.13549754957143 5.335137321099508E-5 84-85 1.0670274642199016E-4 0.0 0.0 8.698781347933114 5.335137321099508E-5 86-87 1.0670274642199016E-4 0.0 0.0 9.329741363212948 5.335137321099508E-5 88-89 1.0670274642199016E-4 0.0 0.0 10.01717380703662 5.335137321099508E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2045 0.0 49.706604 1 GTATCAA 3960 0.0 41.262623 1 TCAACGC 4230 0.0 37.84279 4 ATCAACG 4295 0.0 37.15949 3 AACGCAG 4400 0.0 36.488632 6 CAACGCA 4415 0.0 36.47225 5 TATCAAC 4745 0.0 33.835617 2 ACGCAGA 4955 0.0 32.401615 7 CGCAGAG 5065 0.0 31.510365 8 GCAGAGT 6090 0.0 25.582922 9 GTACATG 5085 0.0 23.446411 1 ACATGGG 4875 0.0 23.28718 3 TACATGG 5445 0.0 21.809 2 ACCGCTC 155 3.908113E-5 21.451612 8 AGAGTAC 5270 0.0 21.00095 10-11 GAGTACT 3260 0.0 20.908743 12-13 CAGAGTA 5885 0.0 20.581987 10-11 TATACCG 165 6.325441E-5 20.151516 5 CATGGGG 2600 0.0 19.730768 4 GTATATA 1595 0.0 17.86834 1 >>END_MODULE