FastQCFastQC Report
Fri 10 Feb 2017
SRR2031523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1874366
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT57030.3042628814223049No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29350.15658628037427055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.11326496532694254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20500.048.2724461
GTATCAA36400.039.211161
TCAACGC39900.034.7610174
ATCAACG41200.033.6641853
CAACGCA42000.033.0229645
AACGCAG42050.032.98376
TATCAAC45050.031.3144042
ACGCAGA48900.028.1690147
CGCAGAG48900.028.0718788
GGGGCGT856.409657E-427.9404626
GTACATG45450.027.844381
ACATGGG42600.026.3138833
TACATGG49950.024.3437212
GCAGAGT57900.023.216169
CATGGGG23550.021.581194
AGAGTAC52000.019.91295210-11
GTATACG1200.00472062119.8234181
GAGTACT36750.019.58113312-13
TTAATAC22550.019.3786623
TAATACT19200.019.2963814
Produced by FastQC (version 0.11.5)