FastQCFastQC Report
Fri 10 Feb 2017
SRR2031521_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031521_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837945
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19510.10615116339172281No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.045.642341
GTATCAA35450.041.1352961
TCAACGC37000.037.486394
CAACGCA38050.036.4519425
ATCAACG38650.036.0089653
AACGCAG39050.035.761756
TATCAAC43600.032.5744552
ACGCAGA44250.031.3445517
CGCAGAG46100.029.8806178
GTACATG52050.028.3812941
TACATGG51650.027.2216132
ACATGGG51400.026.6147173
ATGGGCG1858.976713E-925.675615
GCAGAGT55950.024.6201329
AGAGTAC49050.021.49841510-11
CATGGGG34150.021.4201494
TAAACCG1353.7972053E-421.1110575
CATGGGA21350.020.468334
CGCGGTT704.8546144E-420.3570980-81
CAGAGTA53250.019.31215710-11
Produced by FastQC (version 0.11.5)