##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031521_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1837945 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.257804232444386 34.0 31.0 34.0 30.0 34.0 2 32.416224642195495 34.0 31.0 34.0 31.0 34.0 3 32.521705491731254 34.0 31.0 34.0 31.0 34.0 4 35.74419854783467 37.0 35.0 37.0 35.0 37.0 5 35.73168838022901 37.0 35.0 37.0 35.0 37.0 6 35.85348473430924 37.0 37.0 37.0 35.0 37.0 7 35.87512901637427 37.0 37.0 37.0 35.0 37.0 8 35.92273109369432 37.0 37.0 37.0 35.0 37.0 9 37.750499062812 39.0 39.0 39.0 35.0 39.0 10-11 37.753009475256334 39.0 39.0 39.0 35.0 39.0 12-13 37.72786808092734 39.0 39.0 39.0 35.0 39.0 14-15 39.27051543979825 41.0 40.0 41.0 36.0 41.0 16-17 39.27990255421136 41.0 40.0 41.0 36.0 41.0 18-19 39.22979523326323 41.0 40.0 41.0 36.0 41.0 20-21 39.192467130409234 41.0 40.0 41.0 36.0 41.0 22-23 39.12507283950282 41.0 39.0 41.0 36.0 41.0 24-25 39.08522262635715 41.0 39.0 41.0 36.0 41.0 26-27 38.99364616460231 41.0 39.0 41.0 36.0 41.0 28-29 38.90111156753875 41.0 39.0 41.0 35.0 41.0 30-31 38.76063946418418 41.0 39.0 41.0 35.0 41.0 32-33 38.68058429387169 40.0 39.0 41.0 35.0 41.0 34-35 38.57191428470384 40.0 38.0 41.0 34.5 41.0 36-37 38.45068105955292 40.0 38.0 41.0 34.0 41.0 38-39 38.320499797328 40.0 38.0 41.0 34.0 41.0 40-41 38.21392887164741 40.0 38.0 41.0 33.5 41.0 42-43 38.08543917255413 40.0 38.0 41.0 33.0 41.0 44-45 38.27630641830958 40.0 38.0 41.0 34.0 41.0 46-47 38.19904621737865 40.0 38.0 41.0 33.0 41.0 48-49 38.11237877085549 40.0 38.0 41.0 33.0 41.0 50-51 37.08267412789827 39.0 37.0 40.0 31.5 40.5 52-53 37.15708168633991 39.5 37.5 40.0 32.5 40.5 54-55 37.95559197908534 40.0 38.0 41.0 33.0 41.0 56-57 38.00627657519675 40.0 38.0 41.0 33.0 41.0 58-59 37.83030667402996 40.0 37.0 41.0 33.0 41.0 60-61 37.68139307759481 40.0 37.0 41.0 33.0 41.0 62-63 37.4527717097084 40.0 36.5 41.0 32.5 41.0 64-65 37.20054707839462 39.5 36.0 41.0 32.0 41.0 66-67 36.93700518785927 39.0 35.5 41.0 32.0 41.0 68-69 36.66305275729143 39.0 35.0 41.0 31.5 41.0 70-71 36.32359755052518 38.0 35.0 40.5 31.0 41.0 72-73 35.98392498143307 37.0 35.0 40.0 31.0 41.0 74-75 35.60929734023597 37.0 35.0 39.0 30.5 41.0 76-77 35.234428396932444 36.0 35.0 39.0 30.0 41.0 78-79 34.81905470511903 36.0 35.0 39.0 30.0 40.5 80-81 34.44663224416399 35.0 34.5 37.0 29.0 39.0 82-83 34.10229441033328 35.0 34.0 37.0 29.0 39.0 84-85 33.77005242267859 35.0 34.0 36.5 29.0 38.5 86-87 33.469989036668665 35.0 34.0 36.0 29.0 37.0 88-89 33.21359235450463 35.0 34.0 36.0 28.5 37.0 90-91 32.995542031997694 35.0 34.0 35.5 27.5 36.5 92-93 32.812887219149644 35.0 34.0 35.0 27.0 36.0 94-95 32.65809150981123 35.0 34.0 35.0 27.0 36.0 96-97 32.51447186939761 35.0 34.0 35.0 26.0 36.0 98-99 32.364560963467355 35.0 33.5 35.0 25.5 36.0 100-101 30.792811536797892 34.0 30.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.14373014372377924 1101 2 0.10988826778721261 1101 3 0.18198077615458885 1101 4 -0.0220624517424568 1101 5 0.03037941654175569 1101 6 0.044632332031959265 1101 7 0.05093110037298487 1101 8 0.20586887223299044 1101 9 0.329314692285152 1101 10-11 0.464102347839507 1101 12-13 0.5421713265208083 1101 14-15 0.659388394445287 1101 16-17 0.5134848849914064 1101 18-19 0.6291008633008275 1101 20-21 0.6330918693908245 1101 22-23 0.6182827123848256 1101 24-25 0.6082665529081197 1101 26-27 0.4934201359938868 1101 28-29 0.6637711718803274 1101 30-31 0.7061130011067291 1101 32-33 0.7280282038953345 1101 34-35 0.6835840568373968 1101 36-37 0.6622711435360671 1101 38-39 0.6370136261994048 1101 40-41 0.7005799689292402 1101 42-43 0.5971794526688186 1101 44-45 0.6868835852912909 1101 46-47 0.6531974432597636 1101 48-49 0.6259043139713967 1101 50-51 0.6933143056599107 1101 52-53 0.8055864081944719 1101 54-55 0.7002751612218177 1101 56-57 0.6596007821868923 1101 58-59 0.661113855358451 1101 60-61 0.6013347911604896 1101 62-63 0.632322132493357 1101 64-65 0.6634394261520882 1101 66-67 0.7700092173581439 1101 68-69 0.694610543381124 1101 70-71 0.6415357330785909 1101 72-73 0.5682934337848167 1101 74-75 0.6246860281592888 1101 76-77 0.5516071479474149 1101 78-79 0.658911255957598 1101 80-81 0.6498156263498274 1101 82-83 0.5338922292719914 1101 84-85 0.5756336274930831 1101 86-87 0.6098682918648137 1101 88-89 0.4816139812759843 1101 90-91 0.3809184830233505 1101 92-93 0.34289586473050804 1101 94-95 0.47155589902449435 1101 96-97 0.41356951153302646 1101 98-99 0.35503296991057454 1101 100-101 0.3978329503461566 1102 1 0.239721719139105 1102 2 0.2731882491208921 1102 3 0.38506814130163747 1102 4 0.11013223577651843 1102 5 0.24257167518955214 1102 6 0.2524818589643161 1102 7 0.2964714230080432 1102 8 0.3370875133695179 1102 9 0.40601979358932283 1102 10-11 0.4756116551389411 1102 12-13 0.5331355883801123 1102 14-15 0.6484877105381344 1102 16-17 0.57914227696849 1102 18-19 0.6021741275012928 1102 20-21 0.6572956831683712 1102 22-23 0.5541259354968915 1102 24-25 0.5327033584027632 1102 26-27 0.6461719570850306 1102 28-29 0.6397118534485671 1102 30-31 0.734089998797046 1102 32-33 0.6208345846298897 1102 34-35 0.719149309136391 1102 36-37 0.7299242017772514 1102 38-39 0.7411739676807017 1102 40-41 0.693140145337253 1102 42-43 0.6656590017983604 1102 44-45 0.7851991743712645 1102 46-47 0.7738133869999118 1102 48-49 0.7551922274136302 1102 50-51 0.7796128858947426 1102 52-53 0.6611745548533037 1102 54-55 0.6547461958084924 1102 56-57 0.5866272591317383 1102 58-59 0.7417073995088046 1102 60-61 0.5835033908038127 1102 62-63 0.7304963185403963 1102 64-65 0.7091376254139803 1102 66-67 0.7631917920065803 1102 68-69 0.6506277343916054 1102 70-71 0.6213561427931822 1102 72-73 0.5848988726570994 1102 74-75 0.6169389411179864 1102 76-77 0.5384957414735752 1102 78-79 0.5199562103150654 1102 80-81 0.46003645122146963 1102 82-83 0.4172856096984461 1102 84-85 0.43881593226694093 1102 86-87 0.4072849737119242 1102 88-89 0.3846691301455465 1102 90-91 0.3699801291233271 1102 92-93 0.4286828077487357 1102 94-95 0.49206805350069516 1102 96-97 0.5541021989665325 1102 98-99 0.5114345635199484 1102 100-101 0.46467413590517026 1103 1 0.1609788345341343 1103 2 0.14664664545482253 1103 3 0.18328110936497666 1103 4 0.07102077583461153 1103 5 0.052431942501534934 1103 6 0.04407489752269811 1103 7 0.16020128835865677 1103 8 0.21951507740639897 1103 9 0.16124662484287455 1103 10-11 0.23725750009727875 1103 12-13 0.20674943716166183 1103 14-15 0.19870055151886845 1103 16-17 0.1860778098450666 1103 18-19 0.2914705955447161 1103 20-21 0.2915913390465903 1103 22-23 0.2605230677258845 1103 24-25 0.3495486939567627 1103 26-27 0.30080463243776023 1103 28-29 0.2548788932339292 1103 30-31 0.38013363556097346 1103 32-33 0.299165703712994 1103 34-35 0.20927579659571194 1103 36-37 0.30110880401989704 1103 38-39 0.32462315947564235 1103 40-41 0.3357711220789028 1103 42-43 0.3564260929537255 1103 44-45 0.43460826265653196 1103 46-47 0.36614784513358245 1103 48-49 0.3945179378791934 1103 50-51 0.4241823197551824 1103 52-53 0.3732534874668616 1103 54-55 0.43064565199340876 1103 56-57 0.5169710041395987 1103 58-59 0.5324110836065259 1103 60-61 0.4593926893302296 1103 62-63 0.5396489058123564 1103 64-65 0.5262151999441187 1103 66-67 0.5258453482833616 1103 68-69 0.5959477610431563 1103 70-71 0.6491051206508232 1103 72-73 0.4810708225740328 1103 74-75 0.5844272743536223 1103 76-77 0.517430681894659 1103 78-79 0.3658549102690145 1103 80-81 0.4205256207859023 1103 82-83 0.44652557073241184 1103 84-85 0.46353953879952314 1103 86-87 0.4770996344796927 1103 88-89 0.39251849430744556 1103 90-91 0.4056388801251032 1103 92-93 0.3664711767375408 1103 94-95 0.4154524600182441 1103 96-97 0.37383016165794913 1103 98-99 0.3516266282855369 1103 100-101 0.26046962599429335 1104 1 0.1283760438097019 1104 2 0.16790982134588006 1104 3 0.24272061570746217 1104 4 0.0993630222511328 1104 5 0.015575398136114416 1104 6 0.019701995639046288 1104 7 0.06023028850086121 1104 8 0.15161959296022332 1104 9 0.33027231593303696 1104 10-11 0.31692041139199034 1104 12-13 0.34302174450244394 1104 14-15 0.4478087771735275 1104 16-17 0.40529951153826005 1104 18-19 0.4665879143018188 1104 20-21 0.4456134510558485 1104 22-23 0.3618563952006042 1104 24-25 0.35620374725151294 1104 26-27 0.4221123071696127 1104 28-29 0.4531903339897525 1104 30-31 0.412961716646322 1104 32-33 0.40429820959690943 1104 34-35 0.441115556030347 1104 36-37 0.4866505953818532 1104 38-39 0.42349467047093015 1104 40-41 0.43963856666305645 1104 42-43 0.5495191059167084 1104 44-45 0.5333767152952262 1104 46-47 0.6016855221502837 1104 48-49 0.604426040183391 1104 50-51 0.45326295133514805 1104 52-53 0.5447072414274174 1104 54-55 0.5932481710911617 1104 56-57 0.525642885505853 1104 58-59 0.6560617381469243 1104 60-61 0.5993211716241689 1104 62-63 0.5758323752519559 1104 64-65 0.518660583009634 1104 66-67 0.551094845374557 1104 68-69 0.504787252046718 1104 70-71 0.44294526675402324 1104 72-73 0.3718972707037196 1104 74-75 0.5364250486126778 1104 76-77 0.4436205265721327 1104 78-79 0.4560727149183066 1104 80-81 0.49342183223289027 1104 82-83 0.4447298387863938 1104 84-85 0.30470643901187344 1104 86-87 0.41784467449669194 1104 88-89 0.5445521392658179 1104 90-91 0.39401805179156923 1104 92-93 0.3475705537537763 1104 94-95 0.4567940401505126 1104 96-97 0.31951561157558217 1104 98-99 0.4134961947533853 1104 100-101 0.2884741365949246 1105 1 0.18655792295932372 1105 2 0.12988010428390595 1105 3 0.20557913179177945 1105 4 0.14993381637031433 1105 5 0.14131851097599935 1105 6 0.09569212969369545 1105 7 0.14459418092926768 1105 8 0.05264866173122584 1105 9 0.2277257246767661 1105 10-11 0.12139920780627023 1105 12-13 0.22736477239386232 1105 14-15 0.23703205752584466 1105 16-17 0.18814085163766237 1105 18-19 0.23174525526561496 1105 20-21 0.2869928577578307 1105 22-23 0.20821609236675442 1105 24-25 0.23118996175385575 1105 26-27 0.22916899164565052 1105 28-29 0.14926929135903322 1105 30-31 0.24924253841102484 1105 32-33 0.13442343407697166 1105 34-35 0.27922314252503355 1105 36-37 0.24919204502007375 1105 38-39 0.2942893485236695 1105 40-41 0.20029643296609123 1105 42-43 0.250422987465754 1105 44-45 0.23793244968966576 1105 46-47 0.32808009289969675 1105 48-49 0.38107210311977013 1105 50-51 0.3329962205845405 1105 52-53 0.3380516404169782 1105 54-55 0.31888211769285135 1105 56-57 0.35686163527299186 1105 58-59 0.3114855863127062 1105 60-61 0.41632502411189165 1105 62-63 0.31861473577625077 1105 64-65 0.36876173875594986 1105 66-67 0.4531639740565936 1105 68-69 0.4067129370369216 1105 70-71 0.38062214959219176 1105 72-73 0.34360831176960005 1105 74-75 0.3274922324218039 1105 76-77 0.2908619367167731 1105 78-79 0.4302340350473699 1105 80-81 0.40068008303952496 1105 82-83 0.47070525589084156 1105 84-85 0.3942631793750664 1105 86-87 0.3722731724735766 1105 88-89 0.27667141735283707 1105 90-91 0.3739284833149057 1105 92-93 0.4282933981862911 1105 94-95 0.3803068830181928 1105 96-97 0.41072977859619897 1105 98-99 0.4856031031506163 1105 100-101 0.3279358687985905 1106 1 0.15628952631310966 1106 2 0.16816032557972704 1106 3 0.12476035807872421 1106 4 0.09888172441701215 1106 5 0.14252908029748568 1106 6 0.17591342551290978 1106 7 0.024145135696862496 1106 8 0.19783995354296735 1106 9 0.29726632246693185 1106 10-11 0.24026504071023425 1106 12-13 0.32663145675586946 1106 14-15 0.3302167500172928 1106 16-17 0.3409760091522003 1106 18-19 0.36240503115934786 1106 20-21 0.3905543048201423 1106 22-23 0.36254286281138093 1106 24-25 0.35081253059652795 1106 26-27 0.37231751278378056 1106 28-29 0.38360032950289735 1106 30-31 0.3720757702563873 1106 32-33 0.3453112716545874 1106 34-35 0.3180810645718637 1106 36-37 0.2956022333713406 1106 38-39 0.35578273098759183 1106 40-41 0.38297425810491603 1106 42-43 0.4900496158272176 1106 44-45 0.5908050796853459 1106 46-47 0.4253253450842891 1106 48-49 0.3775070128995708 1106 50-51 0.401977853864274 1106 52-53 0.33284140418421515 1106 54-55 0.3352664396231049 1106 56-57 0.3519569038517929 1106 58-59 0.4218139389287572 1106 60-61 0.34818548075570277 1106 62-63 0.3200818875033704 1106 64-65 0.3541662073493299 1106 66-67 0.4562861603676893 1106 68-69 0.3803749873020834 1106 70-71 0.3960654958847627 1106 72-73 0.33145785975779773 1106 74-75 0.3941427651035312 1106 76-77 0.4023332000077886 1106 78-79 0.3534758210274518 1106 80-81 0.3428938739534928 1106 82-83 0.2872098020314411 1106 84-85 0.2951942664413352 1106 86-87 0.3372629456089342 1106 88-89 0.30879522226693723 1106 90-91 0.3960242359132522 1106 92-93 0.2650086229431068 1106 94-95 0.29460419592902554 1106 96-97 0.31311969017155405 1106 98-99 0.2528016708691112 1106 100-101 0.09284341065587398 1107 1 0.25930567070707156 1107 2 0.23547319737699723 1107 3 0.23226832098097105 1107 4 0.1794758584123599 1107 5 0.17619088334836874 1107 6 0.14925819575976362 1107 7 0.18108066741575612 1107 8 0.19221322629579163 1107 9 0.29769569464674106 1107 10-11 0.24808839699546326 1107 12-13 0.34140381143290455 1107 14-15 0.39249376923755364 1107 16-17 0.2676641249109366 1107 18-19 0.2161296396499992 1107 20-21 0.41081042532539414 1107 22-23 0.3451342605349197 1107 24-25 0.3857883101021997 1107 26-27 0.3482755982522576 1107 28-29 0.3323210931608358 1107 30-31 0.3983416375101214 1107 32-33 0.3630458064493496 1107 34-35 0.314433352735243 1107 36-37 0.43085495793299344 1107 38-39 0.3145971563314802 1107 40-41 0.366719105638758 1107 42-43 0.2272060204987838 1107 44-45 0.3523093265665409 1107 46-47 0.4201221349417352 1107 48-49 0.3566727037203705 1107 50-51 0.23438510947342905 1107 52-53 0.2605741629395055 1107 54-55 0.2421741597348941 1107 56-57 0.29676403767539483 1107 58-59 0.38868153225865143 1107 60-61 0.3382094084962688 1107 62-63 0.4059270480885644 1107 64-65 0.41132930632352327 1107 66-67 0.3997105206031435 1107 68-69 0.35616864274262383 1107 70-71 0.33405683427687904 1107 72-73 0.3691713748326677 1107 74-75 0.3026986888782588 1107 76-77 0.2653551799600109 1107 78-79 0.10859364465697752 1107 80-81 0.11074014309958358 1107 82-83 0.1675896402752315 1107 84-85 0.29214230850418943 1107 86-87 0.27604569499609255 1107 88-89 0.28406631224773093 1107 90-91 0.26901106589748736 1107 92-93 0.18803438917728243 1107 94-95 0.2761402163515285 1107 96-97 0.2872875864040054 1107 98-99 0.31216091095841847 1107 100-101 0.3763217546844757 1108 1 0.23513803559676916 1108 2 0.20584813405568525 1108 3 0.08269903008037005 1108 4 0.15859286130279315 1108 5 0.27253053888041734 1108 6 0.18841870411817752 1108 7 0.2010231567628793 1108 8 0.2265619355342423 1108 9 0.27862480457254435 1108 10-11 0.2877380935497911 1108 12-13 0.3083240116772501 1108 14-15 0.36156099660825447 1108 16-17 0.15099367138133601 1108 18-19 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-0.8155178211783891 2316 62-63 -0.6722272064104331 2316 64-65 -0.9661211881807645 2316 66-67 -0.7378806157655049 2316 68-69 -0.7154332923220252 2316 70-71 -1.0150374464341496 2316 72-73 -0.6915389381698276 2316 74-75 -0.43639316887190915 2316 76-77 -0.5218255674132806 2316 78-79 -0.6180805386950752 2316 80-81 -0.4997578854913556 2316 82-83 -0.5901018837671472 2316 84-85 -0.563438899231663 2316 86-87 -0.5156836107154774 2316 88-89 -0.4705139097835911 2316 90-91 -0.4448524002581564 2316 92-93 -0.3852278247481351 2316 94-95 -0.4953952249577469 2316 96-97 -0.42267367375590936 2316 98-99 -0.46612784540185004 2316 100-101 -0.5096214929641505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 23.0 7 549.0 8 1085.0 9 928.0 10 1091.0 11 1478.0 12 1933.0 13 2195.0 14 2460.0 15 2697.0 16 3064.0 17 3627.0 18 4203.0 19 5304.0 20 6353.0 21 7779.0 22 8568.0 23 9057.0 24 9003.0 25 9132.0 26 9928.0 27 11046.0 28 13117.0 29 15543.0 30 18664.0 31 23334.0 32 30400.0 33 39716.0 34 58400.0 35 92855.0 36 173542.0 37 373386.0 38 613530.0 39 283682.0 40 273.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.88857664402362 20.664220093637187 14.001615935188486 23.445587327150704 2 13.897205846747319 21.403687270293727 42.61552984447304 22.08357703848592 3 17.072110427678737 27.6959321416038 31.66128475008773 23.570672680629727 4 11.320578145700768 18.330363531008818 41.743904197350844 28.60515412593957 5 11.989259743898756 39.1529126279622 36.875096915304866 11.98273071283417 6 27.74854525026592 40.453985293357526 19.774258750941947 12.023210705434602 7 24.874030506897647 33.80525532592107 25.019029405123653 16.301684762057626 8 23.691731798285584 37.44687681078596 22.033901993802864 16.827489397125596 9 25.540100492669804 16.733906618533197 21.792055801452165 35.93393708734484 10-11 22.90569086670167 27.147765575139626 31.25724654437429 18.68929701378442 12-13 23.829412740860036 25.094902186953366 30.48061829924182 20.595066772944783 14-15 21.244705363871063 26.69723522738711 27.640897850588566 24.417161558153264 16-17 20.894749298809266 29.737859402756882 28.196708824257527 21.17068247417632 18-19 20.876005538794686 29.208708639268316 29.585923409024755 20.329362412912246 20-21 21.87671557092296 28.421198675694864 29.268095617659938 20.433990135722233 22-23 21.624205294500108 28.167817861796735 28.942405784721526 21.26557105898163 24-25 21.292584925011358 28.382677392413807 29.06632135346814 21.25841632910669 26-27 21.32683513380433 28.623435412920408 28.859432681609082 21.19029677166618 28-29 21.322672876500658 28.499873500023128 29.099918659154657 21.077534964321565 30-31 21.739225058421226 28.403243840267255 28.88541278438691 20.97211831692461 32-33 21.407931140485704 28.46371899050298 28.918656434224093 21.209693434787223 34-35 21.528092516370183 28.518726077222116 28.83334376164684 21.11983764476086 36-37 21.683945928741068 28.26091096305929 28.833153331574124 21.221989776625524 38-39 21.406434904200072 28.554309296524107 28.886364934750496 21.152890864525325 40-41 21.45700769065451 28.430176093408672 28.990829431783865 21.121986784152956 42-43 21.50360864987799 28.321984607809263 28.79759731656823 21.376809425744515 44-45 21.687727325899306 28.334036110982648 28.57811305561374 21.400123507504304 46-47 21.456716261993034 28.183533539143234 28.73232670018967 21.627423498674062 48-49 21.298862829196953 28.33242243959741 28.79827045355435 21.570444277651294 50-51 21.290326968434854 28.465106409604207 28.617069607632438 21.627497014328505 52-53 21.38701103678293 28.454605551308664 28.625067670686555 21.533315741221852 54-55 21.391907810081367 28.23370666695685 28.67849690823175 21.695888614730038 56-57 21.423628019336814 28.3424422384783 28.503546079996955 21.730383662187933 58-59 21.41097802164918 28.2989425690105 28.513067583632807 21.777011825707515 60-61 21.471153924627778 28.349188903911703 28.53632725680039 21.64332991466012 62-63 21.318429006308676 28.2588162322594 28.61979003724268 21.802964724189245 64-65 21.33752642217259 28.15606560588048 28.668485727266052 21.83792224468088 66-67 21.408257592038936 28.205087747457082 28.385533843504568 22.00112081699942 68-69 21.33616620736747 28.145183887439508 28.471689849260994 22.046960055932033 70-71 21.336139003071363 27.939845860458284 28.62321777855159 22.100797357918765 72-73 21.392043831561878 27.909431457415756 28.549167684560743 22.14935702646162 74-75 21.328984273196422 28.221002260677007 28.27470354118322 22.17530992494335 76-77 21.376292544118566 28.134438190479038 28.358928041916382 22.130341223486013 78-79 21.42931371722222 28.122685934562785 28.22342344303012 22.22457690518487 80-81 21.53138423619858 28.094801531057783 28.11096088294263 22.262853349801002 82-83 21.539572729325414 28.07456153475757 28.206067102116766 22.17979863380025 84-85 21.6887882934473 27.965173060129654 28.14058636139819 22.20545228502485 86-87 21.532635633819293 27.975157036799253 28.23261849511275 22.259588834268705 88-89 21.700458936475247 27.850670177834484 28.134628620551755 22.31424226513851 90-91 21.790178705021095 27.803933197130494 28.211317531264534 22.194570566583874 92-93 21.859468047194014 27.75401331378251 28.22995247409471 22.156566164928766 94-95 21.79610924157143 27.618671940672872 28.213548283544938 22.371670534210764 96-97 21.94448147251414 27.4105590754892 28.278158486788236 22.366800965208427 98-99 22.031644037226357 27.53436582705141 27.971158005272194 22.462832130450042 100-101 22.30268043929497 27.441163908604448 27.972953488814955 22.283202163285626 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16.0 1 19.0 2 33.0 3 55.5 4 54.5 5 59.5 6 98.0 7 164.5 8 289.5 9 368.0 10 356.0 11 364.5 12 395.0 13 443.5 14 505.5 15 575.5 16 650.5 17 735.5 18 861.0 19 969.5 20 1129.0 21 1310.5 22 1644.0 23 2167.5 24 2849.5 25 3810.5 26 5737.5 27 9444.5 28 14929.0 29 22673.0 30 31564.0 31 41428.5 32 51760.5 33 59490.0 34 66431.5 35 71020.0 36 73670.5 37 74512.0 38 71596.0 39 68866.0 40 66975.5 41 64368.0 42 62339.0 43 62186.5 44 61680.5 45 61922.0 46 63203.0 47 62931.5 48 61987.5 49 60722.0 50 58436.0 51 55728.5 52 52523.5 53 49614.0 54 46590.0 55 42789.0 56 38676.0 57 34342.5 58 30867.5 59 27156.5 60 22074.5 61 17416.0 62 13706.5 63 10054.0 64 7112.5 65 5234.5 66 3739.5 67 2754.5 68 2129.0 69 1531.0 70 1086.5 71 836.5 72 603.5 73 397.0 74 298.5 75 231.5 76 163.0 77 110.5 78 84.0 79 59.0 80 46.5 81 38.0 82 31.5 83 29.5 84 21.5 85 17.0 86 13.5 87 9.5 88 9.0 89 8.5 90 6.0 91 3.5 92 3.5 93 3.0 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 1.6322577661464298E-4 48-49 3.5365584933172645E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1837945.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.37823375036077 #Duplication Level Percentage of deduplicated Percentage of total 1 67.88919445657959 34.880269019138424 2 15.8380387585701 16.274609149701767 3 6.562334962974598 10.114835390276218 4 3.3017779577090893 6.785580788118656 5 1.8802661748664633 4.830237752259293 6 1.1566148540051597 3.5654896996929897 7 0.7792722087306594 2.802634078672662 8 0.5300469537681247 2.1786301031492306 9 0.3950270458387063 1.8266212708933978 >10 1.601553618550175 13.290902145864283 >50 0.04856792703471249 1.6893492588278904 >100 0.016557571069567074 1.4009793808145803 >500 5.339359307538605E-4 0.18100085601260668 >1k 2.1357437230154402E-4 0.1788611065780835 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1951 0.10615116339172281 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.72042961024405E-5 0.0 12-13 0.0 0.0 0.0 5.4408592204881E-5 0.0 14-15 0.0 0.0 0.0 5.4408592204881E-5 0.0 16-17 0.0 0.0 0.0 1.63225776614643E-4 0.0 18-19 0.0 0.0 0.0 2.72042961024405E-4 0.0 20-21 0.0 0.0 0.0 2.9924725712684547E-4 0.0 22-23 0.0 0.0 0.0 4.35268737639048E-4 0.0 24-25 0.0 0.0 0.0 8.70537475278096E-4 0.0 26-27 0.0 0.0 0.0 0.0014690319895317868 0.0 28-29 0.0 0.0 0.0 0.005767310773717385 0.0 30-31 0.0 0.0 0.0 0.020348813484625493 0.0 32-33 0.0 0.0 0.0 0.05756429055276409 0.0 34-35 0.0 0.0 0.0 0.11257137727189878 0.0 36-37 0.0 0.0 0.0 0.2050115754279916 0.0 38-39 0.0 0.0 0.0 0.34590262494253093 0.0 40-41 0.0 0.0 0.0 0.5009943170225442 0.0 42-43 0.0 0.0 0.0 0.6526854720897524 0.0 44-45 0.0 0.0 0.0 0.8331587724333427 0.0 46-47 0.0 0.0 0.0 1.0693192668986287 0.0 48-49 0.0 0.0 0.0 1.3224008335396324 0.0 50-51 0.0 0.0 0.0 1.5723811104249583 0.0 52-53 0.0 0.0 0.0 1.8096569810304444 0.0 54-55 0.0 0.0 0.0 2.071253492351512 0.0 56-57 0.0 0.0 0.0 2.3733572005691137 0.0 58-59 0.0 0.0 0.0 2.7079972469252347 0.0 60-61 0.0 0.0 0.0 3.046962776361643 0.0 62-63 0.0 0.0 0.0 3.37744056541409 0.0 64-65 0.0 0.0 0.0 3.704898677599166 0.0 66-67 0.0 0.0 0.0 4.079529039225875 0.0 68-69 0.0 0.0 0.0 4.52067390482305 0.0 70-71 0.0 0.0 0.0 4.956405115495839 0.0 72-73 0.0 0.0 0.0 5.389959982480433 0.0 74-75 0.0 0.0 0.0 5.818482054686076 0.0 76-77 0.0 0.0 0.0 6.270127778578793 0.0 78-79 0.0 0.0 0.0 6.776073277491982 0.0 80-81 0.0 0.0 0.0 7.327885219633885 0.0 82-83 0.0 0.0 0.0 7.878092108305744 0.0 84-85 0.0 0.0 0.0 8.416383515284734 0.0 86-87 0.0 2.72042961024405E-5 0.0 9.003533838063706 0.0 88-89 0.0 5.4408592204881E-5 0.0 9.632061895214491 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1790 0.0 45.64234 1 GTATCAA 3545 0.0 41.135296 1 TCAACGC 3700 0.0 37.48639 4 CAACGCA 3805 0.0 36.451942 5 ATCAACG 3865 0.0 36.008965 3 AACGCAG 3905 0.0 35.76175 6 TATCAAC 4360 0.0 32.574455 2 ACGCAGA 4425 0.0 31.344551 7 CGCAGAG 4610 0.0 29.880617 8 GTACATG 5205 0.0 28.381294 1 TACATGG 5165 0.0 27.221613 2 ACATGGG 5140 0.0 26.614717 3 ATGGGCG 185 8.976713E-9 25.67561 5 GCAGAGT 5595 0.0 24.620132 9 AGAGTAC 4905 0.0 21.498415 10-11 CATGGGG 3415 0.0 21.420149 4 TAAACCG 135 3.7972053E-4 21.111057 5 CATGGGA 2135 0.0 20.46833 4 CGCGGTT 70 4.8546144E-4 20.35709 80-81 CAGAGTA 5325 0.0 19.312157 10-11 >>END_MODULE