FastQCFastQC Report
Fri 10 Feb 2017
SRR2031521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837945
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT31960.17388986068679965No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24640.13406277119282678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18750.10201611038415186No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17950.047.9620551
GTATCAA34500.039.9818231
TCAACGC36950.037.02194
CAACGCA37950.035.9211965
ATCAACG38100.035.904443
AACGCAG39100.035.107656
TATCAAC43500.031.6657262
ACGCAGA43700.031.1947217
CGCAGAG45900.029.596078
ACATGGG43300.026.7659453
GTACATG46950.026.2390331
TACATGG44950.026.2061162
GCAGAGT55650.023.9840139
CATGGGG27600.023.0609074
GTATAAT14400.022.1307411
AGAGTAC47150.021.45747410-11
GAGTACT32250.019.36764112-13
GCGTGCG3203.965397E-1019.29639
CAGAGTA54200.019.27986710-11
CTAAGCG1759.943164E-518.9994353
Produced by FastQC (version 0.11.5)