FastQCFastQC Report
Fri 10 Feb 2017
SRR2031519_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031519_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89790
Sequences flagged as poor quality0
Sequence length101
%GC39

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12681.4121839848535471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11511.281879942087092No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.7283661877714668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5010.557968593384564No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.41986858224746626No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA3380.3764339013253146No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.17596614322307608No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.16594275531796415No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC1470.16371533578349481No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1270.14144114043880165No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1040.1158258157924045No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC970.10802984742176189No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG940.10468871812005791No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA930.10357500835282325No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT910.10134758881835393No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA910.10134758881835393No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG900.10023387905111927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCA353.5533958E-881.428571
CCTTTAG250.005006245357.03
CAGAGCA502.8060489E-547.54
CGCAGAA554.9266695E-543.181829
TTATAGG459.5776445E-442.222222
GTATATG459.5776445E-442.222221
TATCCTA459.5776445E-442.222225
ACCCATG704.265852E-640.7142873
ATAGGTG500.001607889238.04
TAGGTGG500.001607889238.05
TATAGGT500.001607889238.03
ATGGGAG756.8512873E-637.9999965
TACCCAT756.8512873E-637.9999962
GGTATCA10800.036.5046271
TTATCCT550.002567026334.5454564
GAAGTCC550.002567026334.5454566
GAGTGCA352.0464604E-433.92857412-13
AGAAGTC702.0383392E-433.9285745
CCGTGGG300.003942153431.66666854-55
AATAGCA300.003942153431.66666850-51
Produced by FastQC (version 0.11.5)