##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031519_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 89790 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.729168058803875 31.0 30.0 34.0 12.0 34.0 2 28.81612651742956 31.0 30.0 34.0 12.0 34.0 3 28.79249359616884 33.0 28.0 34.0 12.0 34.0 4 31.774874707651186 37.0 32.0 37.0 12.0 37.0 5 31.739458737053123 37.0 32.0 37.0 12.0 37.0 6 31.980855329101235 37.0 33.0 37.0 13.0 37.0 7 32.00102461298586 37.0 33.0 37.0 13.0 37.0 8 32.03585031740728 37.0 33.0 37.0 13.0 37.0 9 33.50214945985076 39.0 34.0 39.0 13.0 39.0 10-11 33.474167501949 39.0 34.0 39.0 13.0 39.0 12-13 33.44180866466199 39.0 34.0 39.0 13.0 39.0 14-15 34.42491368749304 40.0 33.5 41.0 13.0 41.0 16-17 34.393150684931506 40.0 33.5 41.0 13.0 41.0 18-19 34.479207038645725 40.0 34.0 41.0 13.0 41.0 20-21 34.47454059472101 40.0 34.0 41.0 12.5 41.0 22-23 34.35552956899432 40.0 34.0 41.0 12.0 41.0 24-25 34.22052567101014 40.0 34.0 41.0 12.0 41.0 26-27 33.97770353045996 40.0 33.0 41.0 11.5 41.0 28-29 33.73966477336006 39.5 33.0 41.0 10.5 41.0 30-31 33.329491034636376 38.5 32.0 41.0 10.0 41.0 32-33 32.91064706537476 38.0 31.5 41.0 9.0 41.0 34-35 32.6365463860118 38.0 30.5 41.0 8.5 41.0 36-37 32.32004120726139 38.0 30.0 41.0 8.0 41.0 38-39 31.991847644503842 37.5 29.0 41.0 8.0 41.0 40-41 31.6162100456621 37.0 27.0 41.0 8.0 41.0 42-43 31.19193674128522 37.0 25.0 40.0 8.0 41.0 44-45 30.83507072057022 37.0 23.5 41.0 7.5 41.0 46-47 30.207818242565985 37.0 19.5 41.0 7.0 41.0 48-49 29.578577792627243 36.5 15.0 41.0 7.0 41.0 50-51 28.578689163603965 35.5 13.5 40.0 7.0 40.5 52-53 28.540689386345917 35.0 13.0 39.5 7.0 40.5 54-55 28.913993763225303 36.0 13.0 40.5 7.0 41.0 56-57 28.805479452054797 36.0 12.5 41.0 7.0 41.0 58-59 28.518320525671008 35.0 11.5 41.0 7.0 41.0 60-61 28.290605858113373 35.0 10.5 41.0 7.0 41.0 62-63 27.98374540594721 35.0 10.0 40.0 7.0 41.0 64-65 27.67950774028288 35.0 10.0 40.0 7.0 41.0 66-67 27.31683372313175 35.0 10.0 39.5 7.0 41.0 68-69 26.935466087537588 35.0 10.0 39.0 7.0 41.0 70-71 26.5295411515759 34.5 10.0 39.0 7.0 41.0 72-73 26.133929168058803 34.0 10.0 38.0 7.0 41.0 74-75 25.770147009689275 34.0 8.0 37.0 7.0 40.0 76-77 25.44946541931173 34.0 8.0 37.0 7.0 39.5 78-79 25.074468203586147 33.0 8.0 36.0 7.0 39.0 80-81 24.77088205813565 33.0 8.0 36.0 7.0 39.0 82-83 24.46657756988529 32.0 8.0 35.0 7.0 37.0 84-85 24.15753981512418 32.0 8.0 35.0 7.0 37.0 86-87 23.889603519322865 31.5 7.5 35.0 7.0 36.5 88-89 23.643106136540815 31.0 7.0 35.0 7.0 36.0 90-91 23.410129190333 31.0 7.0 35.0 7.0 36.0 92-93 23.15667669005457 30.0 7.0 35.0 7.0 36.0 94-95 22.973644058358392 29.5 7.0 35.0 7.0 35.0 96-97 22.788963136206704 29.0 7.0 35.0 7.0 35.0 98-99 22.60187660095779 29.0 7.0 35.0 7.0 35.0 100-101 21.417858336117607 25.0 7.0 34.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5501517899136665 1101 2 1.4934580550622947 1101 3 1.6282373116621685 1101 4 2.1805818253426743 1101 5 1.9458809212966486 1101 6 1.9087636102160062 1101 7 2.1313588137243435 1101 8 2.487584549519262 1101 9 2.333489570644886 1101 10-11 2.530627972872999 1101 12-13 2.10749984830413 1101 14-15 2.916712204378385 1101 16-17 2.752975026231532 1101 18-19 2.8229978687805684 1101 20-21 2.553932523809806 1101 22-23 2.730765217681572 1101 24-25 2.6565201173833373 1101 26-27 2.57839363620959 1101 28-29 2.4947118268526083 1101 30-31 2.6941463245975754 1101 32-33 3.0190401753251237 1101 34-35 2.5060072737820605 1101 36-37 2.3351105124803695 1101 38-39 2.1934392062996544 1101 40-41 2.0802020283259957 1101 42-43 2.810630607748763 1101 44-45 2.7256798717650703 1101 46-47 3.5133823678875764 1101 48-49 4.239550317456537 1101 50-51 3.8431781870699773 1101 52-53 4.4551373053690035 1101 54-55 3.7740668562540627 1101 56-57 3.6405626201858574 1101 58-59 3.43923871484094 1101 60-61 3.8614284422740077 1101 62-63 4.249457317607064 1101 64-65 4.42573766125577 1101 66-67 4.177839498050723 1101 68-69 4.667547222791995 1101 70-71 4.121325282060685 1101 72-73 3.8765238349005386 1101 74-75 3.1970836464873003 1101 76-77 2.361175711902195 1101 78-79 2.6311448279903935 1101 80-81 2.3293526700428195 1101 82-83 2.2006204130126363 1101 84-85 2.3868451814669918 1101 86-87 2.7770594259787913 1101 88-89 3.025290146504396 1101 90-91 2.6234387913452473 1101 92-93 2.8308639727986566 1101 94-95 2.8901487343368366 1101 96-97 2.695274189728746 1101 98-99 2.8503124507882234 1101 100-101 3.1365035081173005 1102 1 -0.30483724517405264 1102 2 -0.3672875589727944 1102 3 -0.5995587409694103 1102 4 -1.3978172974643464 1102 5 -0.4650291664226529 1102 6 -1.1208416529418912 1102 7 -0.7101982038195196 1102 8 -0.5817685206561762 1102 9 -0.05357183286388789 1102 10-11 0.25033740269756066 1102 12-13 0.10859633953219827 1102 14-15 0.05596659034329576 1102 16-17 0.4896800700911754 1102 18-19 0.28256475474548637 1102 20-21 0.32545116416068254 1102 22-23 0.5551895597868324 1102 24-25 0.26274270510263875 1102 26-27 -0.0572971532640949 1102 28-29 0.40438836194032746 1102 30-31 0.3934336052993288 1102 32-33 0.21805333321986353 1102 34-35 0.18295354571188227 1102 36-37 -0.2183434348880553 1102 38-39 0.31679446945754464 1102 40-41 0.1332447914838859 1102 42-43 0.2374398182750781 1102 44-45 1.322445222642262 1102 46-47 0.4210029819226584 1102 48-49 -0.5038707351750382 1102 50-51 -0.19204659363177612 1102 52-53 -0.21865655428011976 1102 54-55 -0.5234934507634854 1102 56-57 -1.068593081568526 1102 58-59 -0.8909422062116903 1102 60-61 -1.47327550509441 1102 62-63 -0.6652904894104843 1102 64-65 -0.6533467685687917 1102 66-67 -0.5968315545808558 1102 68-69 -0.8558902772080046 1102 70-71 -0.45570322671124686 1102 72-73 -0.05905730545033805 1102 74-75 -1.6048626254425251 1102 76-77 -1.8911543319574555 1102 78-79 -1.673131487799079 1102 80-81 -1.0563381194308654 1102 82-83 -0.6125648940049082 1102 84-85 -0.6424859588838849 1102 86-87 -1.1504350915650718 1102 88-89 -0.3612230113903401 1102 90-91 -0.5768079191810678 1102 92-93 -0.52659216755222 1102 94-95 -0.9041220989964991 1102 96-97 -0.7071380909730074 1102 98-99 -1.391189742194232 1102 100-101 -2.1809307024090145 1103 1 0.9101715576428475 1103 2 0.8853532860976294 1103 3 0.4147458364953778 1103 4 1.5181158011272053 1103 5 0.9656486504787551 1103 6 1.3483484879031806 1103 7 0.7691151764621686 1103 8 1.4048071835691758 1103 9 0.9173788713614641 1103 10-11 0.7436252548102331 1103 12-13 0.834828733898398 1103 14-15 0.5128327875263992 1103 16-17 0.9050629926263909 1103 18-19 0.9684142265764706 1103 20-21 1.1631492275409698 1103 22-23 0.11845980626570451 1103 24-25 0.7071749234124951 1103 26-27 0.6788384101161853 1103 28-29 0.07835403095440796 1103 30-31 0.3421571968486248 1103 32-33 0.6806413613888793 1103 34-35 1.1982484400780749 1103 36-37 1.140151283421801 1103 38-39 0.7820233426969843 1103 40-41 -2.2791978372183053E-4 1103 42-43 -0.3635725056685857 1103 44-45 0.364918814191558 1103 46-47 0.08627586924660235 1103 48-49 0.5190165887686256 1103 50-51 0.3078433711569559 1103 52-53 0.4798469668466403 1103 54-55 0.9770567252928508 1103 56-57 1.3550424818117541 1103 58-59 1.5129970543516897 1103 60-61 1.067877663919674 1103 62-63 1.3890669049557154 1103 64-65 1.846543196219944 1103 66-67 1.640404360912104 1103 68-69 1.2520542650455155 1103 70-71 1.2852738859648092 1103 72-73 0.8166029058172661 1103 74-75 1.0963917759659232 1103 76-77 1.9907778863524044 1103 78-79 1.9980833009333132 1103 80-81 1.2607833594423745 1103 82-83 1.3331877468401636 1103 84-85 0.8903045340738593 1103 86-87 1.2880552253363362 1103 88-89 1.46228050973642 1103 90-91 1.6427123272978044 1103 92-93 1.137140226813976 1103 94-95 1.5427308939612452 1103 96-97 1.9557140217030486 1103 98-99 1.6191535676649202 1103 100-101 1.3670446497036615 1104 1 -0.5006570503688579 1104 2 -0.3632291174143525 1104 3 -0.805118805904474 1104 4 -0.7310153494123988 1104 5 -0.7071152053836904 1104 6 -0.47879619839643794 1104 7 -1.0953443077156244 1104 8 -0.9127344297470863 1104 9 -1.3222406640327193 1104 10-11 -1.525535162237503 1104 12-13 -0.9409166474807904 1104 14-15 -1.0893256174489139 1104 16-17 -1.2971502870516858 1104 18-19 -2.007715277722049 1104 20-21 -1.0804944527224336 1104 22-23 -1.225147290862516 1104 24-25 -1.7619123273648967 1104 26-27 -1.5712987766407167 1104 28-29 -0.9804533588388935 1104 30-31 -0.98379853755781 1104 32-33 -1.0120603031437732 1104 34-35 -1.1099644737686383 1104 36-37 -1.0367281751477933 1104 38-39 -1.509910075997002 1104 40-41 -1.1621082929316984 1104 42-43 -0.6702306362703752 1104 44-45 -0.9740239332018952 1104 46-47 -0.8182421479474691 1104 48-49 -0.7912083975127011 1104 50-51 -0.6022535741512591 1104 52-53 -0.9325364244099923 1104 54-55 -1.2906403663479011 1104 56-57 -1.4405492503996946 1104 58-59 -0.6509868490688326 1104 60-61 -1.0948258297697357 1104 62-63 -0.90615899590399 1104 64-65 -0.5051121906467166 1104 66-67 -0.7685036325029309 1104 68-69 -0.9818034265586562 1104 70-71 -0.8102080968411194 1104 72-73 -0.43060763012566383 1104 74-75 -0.4272043462217461 1104 76-77 -0.4328548189704442 1104 78-79 -0.5858749942925847 1104 80-81 -0.865794288262034 1104 82-83 -0.8186322641347772 1104 84-85 -0.9527538160267426 1104 86-87 -0.4041891500066299 1104 88-89 -0.3981548295721602 1104 90-91 -0.9476480165836669 1104 92-93 -0.5152285311885834 1104 94-95 -1.2288988847107838 1104 96-97 -0.9709367922717114 1104 98-99 -0.8057595473890373 1104 100-101 -1.8826352478635613 1105 1 1.8361833244461998 1105 2 1.2585035802677105 1105 3 1.2851327147267924 1105 4 1.548780803801474 1105 5 1.7369091247165898 1105 6 1.547671005285956 1105 7 0.6541214164336431 1105 8 0.9963564793438238 1105 9 1.508928167136112 1105 10-11 1.6912986368747767 1105 12-13 1.8941976053549823 1105 14-15 1.536188109330638 1105 16-17 1.6739166207240856 1105 18-19 1.5120940268973797 1105 20-21 1.8022114489708088 1105 22-23 1.619172945862779 1105 24-25 1.3085298886469445 1105 26-27 2.007774049267546 1105 28-29 1.8352625708010848 1105 30-31 2.2856408837803386 1105 32-33 1.9470881433464413 1105 34-35 2.624705919129603 1105 36-37 2.7794809322005563 1105 38-39 2.8583292795841295 1105 40-41 2.7324338737623712 1105 42-43 3.126086970173816 1105 44-45 2.2767569315030194 1105 46-47 2.691374817365702 1105 48-49 2.9344204040654667 1105 50-51 3.428898817760629 1105 52-53 3.405947876099628 1105 54-55 3.308488353033976 1105 56-57 3.449800905773241 1105 58-59 3.580477888725021 1105 60-61 3.6918748113612843 1105 62-63 3.2965370422350837 1105 64-65 3.442282187127411 1105 66-67 3.353722242887496 1105 68-69 3.12324342532364 1105 70-71 3.7877698745545736 1105 72-73 3.5016626312585224 1105 74-75 3.097372342911907 1105 76-77 3.4022594338653285 1105 78-79 3.0092261071376285 1105 80-81 2.530884981834955 1105 82-83 2.5768140616912945 1105 84-85 2.8917308132680155 1105 86-87 2.8314675983083433 1105 88-89 2.6288877481033275 1105 90-91 2.9535520491733642 1105 92-93 2.6585344147262617 1105 94-95 2.828967299737677 1105 96-97 2.5974505166219295 1105 98-99 3.1450048780589306 1105 100-101 3.4729458798694637 1106 1 0.2921920758136025 1106 2 -0.6566394108246456 1106 3 -0.20931954343854642 1106 4 0.20982159142454293 1106 5 0.2701174385156193 1106 6 0.22715217421860245 1106 7 -0.10255931493063031 1106 8 -0.1271003107796318 1106 9 0.36078001898796686 1106 10-11 -0.5161556528579965 1106 12-13 -0.14526168515915572 1106 14-15 -0.5358543973110201 1106 16-17 -0.42148891139030553 1106 18-19 -0.36413663414339936 1106 20-21 0.017870145642163493 1106 22-23 -0.010300409348971584 1106 24-25 -0.025741091193651755 1106 26-27 0.6076334022914622 1106 28-29 -0.7164642615164638 1106 30-31 -0.05699077741672198 1106 32-33 -0.2645855556690222 1106 34-35 -0.46702716416466217 1106 36-37 -1.5001721385917577 1106 38-39 -1.0570481231350506 1106 40-41 -0.4357173998741395 1106 42-43 -0.27124073728047904 1106 44-45 -0.20706866624662723 1106 46-47 0.3424921794535223 1106 48-49 -0.6165250561626934 1106 50-51 -0.23901804424906103 1106 52-53 0.2030255444853104 1106 54-55 -0.3318460742202767 1106 56-57 0.14186216534505647 1106 58-59 0.14619128144263271 1106 60-61 0.31009640848583686 1106 62-63 0.6065073500956899 1106 64-65 0.941657089455898 1106 66-67 0.5378135071475398 1106 68-69 -0.25220586362775776 1106 70-71 0.010539057239370209 1106 72-73 0.5360430031916366 1106 74-75 0.2256736399895729 1106 76-77 -0.05695294306856624 1106 78-79 -0.30777654952747113 1106 80-81 -0.05498781078889081 1106 82-83 -1.0164036285728102 1106 84-85 -0.8240060206122806 1106 86-87 -0.24698215946630597 1106 88-89 -0.7342939990446631 1106 90-91 -0.5786404809094599 1106 92-93 -0.021962537922590286 1106 94-95 -0.7394808952927967 1106 96-97 -0.9980331527013995 1106 98-99 -0.2536511619473174 1106 100-101 -0.13347699870531216 1107 1 -2.1045531542649627 1107 2 -2.441151195336431 1107 3 -2.285638286423957 1107 4 -2.6790672974643464 1107 5 -2.7704268936953795 1107 6 -1.7174325620328013 1107 7 -2.6619027492740663 1107 8 -2.0831889752016295 1107 9 -3.081980923772978 1107 10-11 -3.034463733666076 1107 12-13 -2.465267296831435 1107 14-15 -2.0406243187476107 1107 16-17 -2.4286437935451843 1107 18-19 -2.4013841088908805 1107 20-21 -2.0431567903847707 1107 22-23 -2.4426797583949877 1107 24-25 -3.087399340351908 1107 26-27 -3.1638312441731884 1107 28-29 -2.782401410786946 1107 30-31 -1.698895940155218 1107 32-33 -1.7620603031437732 1107 34-35 -2.7268475906517544 1107 36-37 -1.745332071251692 1107 38-39 -2.0894555305424554 1107 40-41 -2.481101799425204 1107 42-43 -2.8065942726340154 1107 44-45 -2.335225231903191 1107 46-47 -2.216781108986435 1107 48-49 -1.310688916993218 1107 50-51 -1.7637795481772294 1107 52-53 -1.6646792815528464 1107 54-55 -1.1818741325816653 1107 56-57 -1.1864908088412527 1107 58-59 -1.2524478880298737 1107 60-61 -1.1337868687307733 1107 62-63 -1.4678473075923044 1107 64-65 -1.2023524503869716 1107 66-67 -1.1280815545808558 1107 68-69 -0.2585891408443679 1107 70-71 -0.34916913580215336 1107 72-73 -0.7877504872685179 1107 74-75 -1.3143796708970719 1107 76-77 -0.4572054683210922 1107 78-79 0.017209421291831006 1107 80-81 -0.6344631194308654 1107 82-83 -0.2269114849140017 1107 84-85 -0.9592473225202482 1107 86-87 -0.4366566824741618 1107 88-89 -1.26747301139034 1107 90-91 -0.2877454191810678 1107 92-93 -0.28227398573404017 1107 94-95 0.1761335828216808 1107 96-97 0.6451346362997192 1107 98-99 -0.42670110583059895 1107 100-101 -0.803089793318108 1108 1 0.619755146130295 1108 2 0.8147776584185102 1108 3 0.4554684329436327 1108 4 0.49484574601391174 1108 5 0.8535849640121285 1108 6 0.9688322601015855 1108 7 0.38898657878917575 1108 8 0.4746173489090424 1108 9 0.7806672975708935 1108 10-11 0.32744338095842807 1108 12-13 0.32870503518437033 1108 14-15 0.23803180773459331 1108 16-17 0.23934039617812886 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-0.39494971560646164 2316 76-77 -0.35977625818696524 2316 78-79 -0.6900372255039962 2316 80-81 -0.004340168611193462 2316 82-83 -0.38702698416884473 2316 84-85 -0.6447912867527386 2316 86-87 -0.45230496861424996 2316 88-89 -0.16603858516083037 2316 90-91 -0.4076224683613958 2316 92-93 -0.29913315115877737 2316 94-95 0.0445203190362875 2316 96-97 0.6360996139450243 2316 98-99 1.1469762414123252 2316 100-101 1.1833111008696733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 48.0 7 1013.0 8 2098.0 9 1526.0 10 1517.0 11 1602.0 12 1732.0 13 1631.0 14 1443.0 15 1334.0 16 1468.0 17 1694.0 18 1883.0 19 2329.0 20 2825.0 21 3100.0 22 2378.0 23 1878.0 24 1605.0 25 1260.0 26 1126.0 27 1140.0 28 1211.0 29 1043.0 30 1081.0 31 1261.0 32 1425.0 33 1725.0 34 2180.0 35 3602.0 36 5266.0 37 10046.0 38 17859.0 39 6459.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46586479563426 23.566098674685378 15.25671010134759 17.71132642833278 2 16.362623900211602 25.121951219512194 39.64138545495044 18.87403942532576 3 17.76478449716004 33.7821583695289 31.918921928945316 16.534135204365743 4 12.54371310836396 22.920147009689277 37.978616772469096 26.55752310947767 5 10.919924267735828 40.36863793295467 33.85232208486468 14.859115714444815 6 22.37665664327876 40.79630248357278 19.381891079184765 17.445149793963694 7 20.770687158926386 39.59460964472659 24.194230983405724 15.440472212941309 8 24.383561643835616 34.43367858336118 22.006905000556856 19.175854772246353 9 28.12562646174407 17.83717563203029 21.715113041541375 32.322084864684264 10-11 22.00801871032409 31.05022831050228 26.76634369083417 20.17540928833946 12-13 25.155919367412853 30.855885956119835 27.300924379106807 16.687270297360506 14-15 23.060474440360842 30.61922263058247 28.3066042989197 18.013698630136986 16-17 17.792627241340906 33.49983294353491 29.404165274529458 19.30337454059472 18-19 17.893417975275643 29.9871923376768 34.56064149682593 17.55874819022163 20-21 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33.21472324312284 18.40739503285444 46-47 17.244125180977836 31.525225526227864 32.52310947767012 18.70753981512418 48-49 17.12050339681479 31.533021494598508 31.982403385677692 19.36407172290901 50-51 16.96179975498385 31.121505735605304 31.533021494598508 20.383673014812338 52-53 16.63492593830048 31.208931952333224 31.7513086089765 20.4048335003898 54-55 17.158369528900767 31.11482347700189 30.62033634034971 21.106470653747632 56-57 17.154471544715445 30.976723465864797 30.116939525559637 21.751865463860117 58-59 16.940639269406393 29.72714110702751 30.683260942198466 22.64895868136764 60-61 17.575453836730148 29.217062033634033 30.818019824033854 22.38946430560196 62-63 17.171177191223965 28.945316850428775 31.55975052901214 22.323755429335115 64-65 17.60663771021272 28.477558748190223 31.071945650963357 22.8438578906337 66-67 17.548724802316517 29.18532130526785 30.414300033411294 22.851653859004344 68-69 17.652856665552957 28.818353936964026 30.906002895645397 22.622786501837624 70-71 17.633923599509966 28.278761554738836 31.314734380220514 22.772580465530684 72-73 17.161153803318854 27.834391357612205 31.287448490923264 23.717006348145674 74-75 17.65229981066934 27.28755986189999 30.944982737498606 24.115157589932064 76-77 17.413409065597506 27.525893752088205 31.38434124067268 23.676355941641607 78-79 17.937966365965032 27.304822363292125 30.987303708653524 23.769907562089323 80-81 17.85610869807328 27.4935961688384 30.659316182202918 23.9909789508854 82-83 18.280988974273306 27.43791068047667 30.224969372981402 24.056130972268626 84-85 18.142888963136205 26.87437353825593 30.10580242788729 24.87693507072057 86-87 17.931284107361623 27.303151798641274 30.00055685488362 24.765007239113487 88-89 19.113487025281213 26.1599287225749 29.961577013030404 24.765007239113487 90-91 19.358503174072837 25.97393919144671 29.93985967256933 24.727697961911126 92-93 19.719345138656866 25.84029401937855 29.66310279541152 24.77725804655307 94-95 19.48045439358503 25.08853992649516 29.66644392471322 25.764561755206593 96-97 20.31517986412741 24.854660875375878 29.03664105134202 25.79351820915469 98-99 20.15814678694732 24.888629023276536 29.367969707094332 25.585254482681812 100-101 20.903775476110926 24.491591491257378 29.56231206147678 25.04232097115492 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 388.0 1 235.5 2 83.0 3 77.0 4 61.5 5 69.0 6 109.5 7 172.5 8 239.5 9 259.5 10 198.5 11 147.5 12 156.5 13 164.0 14 158.0 15 150.5 16 148.0 17 160.0 18 180.5 19 211.0 20 250.5 21 296.0 22 363.5 23 462.5 24 572.0 25 681.0 26 898.5 27 1212.5 28 1470.5 29 1685.0 30 2106.5 31 2528.5 32 3003.0 33 3489.5 34 3771.5 35 4203.0 36 4885.0 37 5219.0 38 4616.5 39 3814.0 40 3272.0 41 2878.0 42 2582.5 43 2406.5 44 2459.0 45 2528.0 46 2218.5 47 1867.0 48 1806.0 49 1847.5 50 2081.0 51 2165.5 52 1835.5 53 1389.5 54 1085.0 55 967.0 56 893.0 57 753.0 58 652.0 59 589.5 60 559.5 61 546.0 62 492.0 63 430.5 64 362.5 65 317.0 66 292.0 67 244.5 68 226.5 69 211.5 70 174.5 71 148.0 72 127.0 73 106.0 74 98.0 75 88.5 76 75.0 77 65.5 78 49.0 79 33.5 80 29.5 81 26.0 82 16.0 83 15.5 84 15.0 85 13.0 86 12.0 87 10.0 88 8.5 89 6.0 90 4.0 91 2.0 92 1.5 93 1.5 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 89790.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.97171177191223 #Duplication Level Percentage of deduplicated Percentage of total 1 84.55737509202697 47.32821026840405 2 6.375231311061145 7.136652188439693 3 2.7896611417315 4.684263280988974 4 1.5281453329884394 3.4213164049448714 5 1.0685078695505104 2.9903107250250587 6 0.6606044929064607 2.21850985633144 7 0.4357601926099847 1.707317073170732 8 0.38601587838510054 1.7284775587481902 9 0.30642497562528603 1.5436017373872368 >10 1.7748771315438643 18.01536919478784 >50 0.09550908331177746 3.6863793295467198 >100 0.013928407982967546 1.5591936741285222 >500 0.0039795451379907275 1.2863347811560308 >1k 0.0039795451379907275 2.6940639269406392 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1268 1.4121839848535471 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1151 1.281879942087092 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 654 0.7283661877714668 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 501 0.557968593384564 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 377 0.41986858224746626 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 338 0.3764339013253146 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 158 0.17596614322307608 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 149 0.16594275531796415 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 147 0.16371533578349481 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 127 0.14144114043880165 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 104 0.1158258157924045 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 97 0.10802984742176189 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 94 0.10468871812005791 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 93 0.10357500835282325 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 91 0.10134758881835393 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 91 0.10134758881835393 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 90 0.10023387905111927 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0011137097672346587 0.0 9 0.0 0.0 0.0 0.0011137097672346587 0.0 10-11 0.0 0.0 0.0 0.0011137097672346587 0.0 12-13 0.0 0.0 0.0 0.0011137097672346587 0.0 14-15 0.0 0.0 0.0 0.0022274195344693173 0.0 16-17 0.0 0.0 0.0 0.0027842744180866467 0.0 18-19 0.0 0.0 0.0 0.004454839068938635 0.0 20-21 0.0 0.0 0.0 0.010023387905111928 0.001670564650851988 22-23 0.0 0.0 0.0 0.0322975832498051 0.0022274195344693173 24-25 0.0 0.0 0.0 0.07795968370642611 0.0022274195344693173 26-27 0.0 0.0 0.0 0.14199799532241897 0.0022274195344693173 28-29 0.0 0.0 0.0 0.2851097004120726 0.0022274195344693173 30-31 0.0 0.0 0.0 0.4939302817685711 0.0022274195344693173 32-33 0.0 0.0 0.0 0.7807105468314957 0.0022274195344693173 34-35 0.0 0.0 0.0 1.3364517206815902 0.005568548836173293 36-37 0.0 0.0 0.0 2.1043546051898874 0.005568548836173293 38-39 0.0 0.0 0.0 2.8778260385343577 0.006682258603407952 40-41 0.0 0.0 0.0 3.539369640271745 0.006682258603407952 42-43 0.0 0.0 0.0 4.041652745294576 0.006682258603407952 44-45 0.0 0.0 0.0 4.501058024278873 0.006682258603407952 46-47 0.0 0.0 0.0 5.080187103240895 0.007239113487025281 48-49 0.0 0.0 0.0 5.590823031517987 0.007795968370642611 50-51 0.0 0.0 0.0 6.03407951887738 0.00890967813787727 52-53 0.0 0.0 0.0 6.415525114155251 0.00890967813787727 54-55 0.0 0.0 0.0 6.894420314066155 0.010023387905111928 56-57 0.0 0.0 0.0 7.54594052789843 0.010580242788729257 58-59 0.0 0.0 0.0 8.041541374317852 0.011693952555963916 60-61 0.0 0.0 0.0 8.479786167724692 0.012250807439581245 62-63 0.0 0.0 0.0 8.887960797416193 0.013364517206815904 64-65 0.0 0.0 0.0 9.332330994542822 0.013364517206815904 66-67 0.0 0.0 0.0 9.84964918142332 0.013364517206815904 68-69 0.0 0.0 0.0 10.418754872480232 0.013364517206815904 70-71 0.0 0.0 0.0 10.966700077959683 0.013921372090433233 72-73 0.0 0.0 0.0 11.4717674574006 0.015035081857667892 74-75 0.0 0.0 0.0 11.99576790288451 0.01614879162490255 76-77 0.0 0.0 0.0 12.61443367858336 0.01670564650851988 78-79 0.0 0.0 0.0 13.209154694286669 0.01781935627575454 80-81 0.0 0.0 0.0 13.84062813230872 0.01781935627575454 82-83 0.0 0.0 0.0 14.457623343356723 0.01781935627575454 84-85 0.0 0.0 0.0 15.080187103240895 0.01781935627575454 86-87 0.0 5.568548836173293E-4 0.0 15.709433121728477 0.018376211159371866 88-89 0.0 0.0011137097672346587 0.0 16.391580354159707 0.018933066042989197 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 35 3.5533958E-8 81.42857 1 CCTTTAG 25 0.0050062453 57.0 3 CAGAGCA 50 2.8060489E-5 47.5 4 CGCAGAA 55 4.9266695E-5 43.18182 9 TTATAGG 45 9.5776445E-4 42.22222 2 GTATATG 45 9.5776445E-4 42.22222 1 TATCCTA 45 9.5776445E-4 42.22222 5 ACCCATG 70 4.265852E-6 40.714287 3 ATAGGTG 50 0.0016078892 38.0 4 TAGGTGG 50 0.0016078892 38.0 5 TATAGGT 50 0.0016078892 38.0 3 ATGGGAG 75 6.8512873E-6 37.999996 5 TACCCAT 75 6.8512873E-6 37.999996 2 GGTATCA 1080 0.0 36.504627 1 TTATCCT 55 0.0025670263 34.545456 4 GAAGTCC 55 0.0025670263 34.545456 6 GAGTGCA 35 2.0464604E-4 33.928574 12-13 AGAAGTC 70 2.0383392E-4 33.928574 5 CCGTGGG 30 0.0039421534 31.666668 54-55 AATAGCA 30 0.0039421534 31.666668 50-51 >>END_MODULE