FastQCFastQC Report
Fri 10 Feb 2017
SRR2031519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89790
Sequences flagged as poor quality0
Sequence length101
%GC38

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT79408.84285555184319No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT25292.8165720013364517TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT17411.9389687047555406RNA PCR Primer, Index 11 (95% over 23bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG15191.6917251364294463TruSeq Adapter, Index 11 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14291.591491257378327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12981.445595277870587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7550.8408508742621673No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT6540.7283661877714668TruSeq Adapter, Index 11 (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4860.5412629468760441No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4740.5278984296692282No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA4690.5223298808330549RNA PCR Primer, Index 11 (96% over 28bp)
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG3790.4220960017819356TruSeq Adapter, Index 11 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC2960.32965809110145894No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA2810.3129524445929391No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2040.22719679251587038No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC2000.22274195344693173No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT1910.2127185655418198No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC1900.21160485577458513RNA PCR Primer, Index 17 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1700.18933066042989197No Hit
CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1340.14923710880944424No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC1330.1481233990422096No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGT1190.13253146230092439No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1180.13141775253368973No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1120.12473549393028176No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.1213943646285778No Hit
CTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC1080.12028065486134315No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT1000.11137097672346587No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT950.10580242788729256No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA950.10580242788729256No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA920.10246129858558858No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT900.10023387905111927No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGTG200.002067832771.257
CATGAGG250.005006245357.06
CTATACA250.005006245357.01
GGATATG250.005006245357.01
TGGGAGT250.005006245357.06
GTACCCA502.8060489E-547.51
ACCCATG502.8060489E-547.53
TACCCAT554.9266695E-543.181822
GGGGCAG704.265852E-640.7142877
TGGGGCA704.265852E-640.7142876
TGCTTAT250.001612185238.012-13
ATGGGGC801.0665901E-535.6255
ATCTTTA300.003942153431.66666886-87
CTTATAC22850.030.7658631
GGTATCA12200.030.7581961
TATACAC23300.030.1716733
TTATACA23350.030.1070692
GTATCAA17250.029.4637681
AACGCAG16950.029.1445436
GGGCAGT1004.903099E-528.58
Produced by FastQC (version 0.11.5)