##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031519_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 89790 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46185544047221 34.0 31.0 34.0 31.0 34.0 2 32.62383338901882 34.0 31.0 34.0 31.0 34.0 3 32.647733600623674 34.0 31.0 34.0 31.0 34.0 4 36.16608753758771 37.0 35.0 37.0 35.0 37.0 5 36.08665775698853 37.0 35.0 37.0 35.0 37.0 6 36.14592939080076 37.0 37.0 37.0 35.0 37.0 7 36.14092883394587 37.0 36.0 37.0 35.0 37.0 8 36.16955117496381 37.0 37.0 37.0 35.0 37.0 9 37.975877046441695 39.0 39.0 39.0 35.0 39.0 10-11 37.90905446040762 39.0 38.0 39.0 35.0 39.0 12-13 37.89099008798307 39.0 38.0 39.0 35.0 39.0 14-15 39.182475776812566 41.0 39.0 41.0 36.0 41.0 16-17 39.053135092994765 41.0 38.5 41.0 35.5 41.0 18-19 39.14983294353492 41.0 39.0 41.0 35.5 41.0 20-21 39.22722463526005 41.0 39.0 41.0 35.0 41.0 22-23 39.167490811894424 41.0 39.0 41.0 35.0 41.0 24-25 39.06059694843523 41.0 38.5 41.0 35.0 41.0 26-27 38.76092549281657 40.5 38.0 41.0 35.0 41.0 28-29 38.487008575565206 40.0 38.0 41.0 35.0 41.0 30-31 37.99521104800089 40.0 36.5 41.0 34.0 41.0 32-33 36.85061810892081 39.0 35.0 40.0 31.5 41.0 34-35 36.9379775030627 39.0 35.0 41.0 32.5 41.0 36-37 36.9149626907228 39.5 35.0 41.0 32.5 41.0 38-39 36.25414300033411 39.0 35.0 41.0 29.5 41.0 40-41 36.18526005123065 39.0 35.0 41.0 29.5 41.0 42-43 35.88590043434681 39.0 35.0 41.0 27.0 41.0 44-45 35.411109254928164 39.0 35.0 41.0 23.5 41.0 46-47 34.76386011805323 39.0 35.0 41.0 17.5 41.0 48-49 34.23664661989086 39.0 35.0 41.0 11.0 41.0 50-51 33.523989308386234 39.0 33.0 41.0 9.0 41.0 52-53 33.66023499276089 39.0 34.0 41.0 8.0 41.0 54-55 33.22446263503731 37.5 33.5 41.0 8.0 41.0 56-57 32.759349593495934 37.0 32.5 41.0 7.0 41.0 58-59 32.33406281323087 37.0 31.0 41.0 7.0 41.0 60-61 31.559628020937744 36.5 28.0 41.0 7.0 41.0 62-63 30.848713665218845 36.0 25.5 40.5 7.0 41.0 64-65 30.171333110591377 36.0 21.5 40.0 7.0 41.0 66-67 29.149688161265175 35.0 18.0 40.0 7.0 41.0 68-69 27.885182091546945 35.0 12.5 39.0 7.0 41.0 70-71 26.839937632253033 35.0 10.0 39.0 7.0 41.0 72-73 26.133840071277426 34.5 9.5 38.5 7.0 41.0 74-75 25.795600846419426 34.0 10.0 37.0 7.0 40.0 76-77 24.82914578460853 32.0 10.0 35.5 7.0 39.0 78-79 25.413832275309055 33.5 10.0 36.0 7.0 39.0 80-81 25.310017819356275 34.0 10.0 36.0 7.0 39.0 82-83 25.087598841741844 34.0 10.0 35.5 7.0 37.0 84-85 24.85877046441697 33.0 10.0 35.0 7.0 37.0 86-87 24.650879830716114 33.0 8.0 35.0 7.0 36.5 88-89 24.461415525114155 33.0 8.0 35.0 7.0 36.0 90-91 24.312145005011693 33.0 8.0 35.0 7.0 36.0 92-93 24.17292014700969 33.0 8.0 35.0 7.0 36.0 94-95 24.084391357612205 33.0 8.0 35.0 7.0 35.0 96-97 24.017618888517653 33.0 8.0 35.0 7.0 35.0 98-99 23.95742287559862 33.0 8.0 35.0 7.0 35.0 100-101 23.117919590154806 29.5 7.5 34.5 7.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8521167877075726 1101 2 0.6090567914610006 1101 3 0.6259042812211888 1101 4 0.5509793925869388 1101 5 0.49153198211038784 1101 6 0.5651983798344062 1101 7 0.5385140958052688 1101 8 0.45938323019691296 1101 9 0.48240055684745897 1101 10-11 0.6618730154083323 1101 12-13 0.7485679126185332 1101 14-15 1.1212281918190925 1101 16-17 0.9010631647423679 1101 18-19 0.9126595026516497 1101 20-21 0.8239248252391178 1101 22-23 0.9575216751749451 1101 24-25 0.9820762864292334 1101 26-27 1.0577670398660999 1101 28-29 0.9096476551852177 1101 30-31 0.843398329844284 1101 32-33 1.5999534982051173 1101 34-35 1.5016938178462667 1101 36-37 1.3672331142087728 1101 38-39 2.123088046950194 1101 40-41 1.4076654869137073 1101 42-43 1.3978777976473893 1101 44-45 1.2107472744002408 1101 46-47 0.7374843387476844 1101 48-49 0.648630700682908 1101 50-51 0.39293125846352694 1101 52-53 0.5814581694839589 1101 54-55 1.3407512923774334 1101 56-57 1.5589561387073587 1101 58-59 1.7176152215059588 1101 60-61 1.2015785946067048 1101 62-63 1.598452690789241 1101 64-65 1.8233405325294427 1101 66-67 2.0115292608171096 1101 68-69 2.227876841482896 1101 70-71 2.8347395570994074 1101 72-73 2.828572446071618 1101 74-75 2.779785180241163 1101 76-77 2.542089926489897 1101 78-79 2.2484949249760113 1101 80-81 2.593776914841502 1101 82-83 2.1627482903978326 1101 84-85 1.3215282279007354 1101 86-87 0.7134412249846811 1101 88-89 1.2161137352909428 1101 90-91 1.299764922424398 1101 92-93 1.5583902592551588 1101 94-95 1.6975455927751746 1101 96-97 1.8541216177630773 1101 98-99 0.8589597739743411 1101 100-101 1.1482098849891784 1102 1 -0.06317926492400971 1102 2 0.23652389672415808 1102 3 0.2163648075369764 1102 4 -0.04715657232534198 1102 5 -0.12634082490715315 1102 6 0.314650134220372 1102 7 0.008634709840357857 1102 8 0.21459156353024866 1102 9 0.03612862702289732 1102 10-11 0.4815002083907842 1102 12-13 0.4345602371799373 1102 14-15 0.6971602093629556 1102 16-17 0.2878504454441213 1102 18-19 0.7440739763358621 1102 20-21 -0.18457298177842318 1102 22-23 0.08101399973634926 1102 24-25 -0.2470903802374309 1102 26-27 -0.018439100484776816 1102 28-29 0.013266076237847813 1102 30-31 -0.1176762315592228 1102 32-33 0.44356643680161056 1102 34-35 0.21263131784626665 1102 36-37 -0.7199379384228024 1102 38-39 -0.020415242523490917 1102 40-41 -0.07821718852489568 1102 42-43 -0.025093693580679144 1102 44-45 0.46417380948795994 1102 46-47 -0.47317903844530207 1102 48-49 -1.038869299317092 1102 50-51 -0.1217343117119114 1102 52-53 -0.6727907340248152 1102 54-55 -0.7586182251664297 1102 56-57 0.12310087554946136 1102 58-59 0.31438057238315054 1102 60-61 -0.14559794048101438 1102 62-63 -0.008181081140580915 1102 64-65 -0.0977120990495024 1102 66-67 0.309500752045178 1102 68-69 0.2846202625355261 1102 70-71 -0.32219574991813715 1102 72-73 -0.08055584340206678 1102 74-75 -0.1497652583553304 1102 76-77 0.44916229491095194 1102 78-79 -0.2989283206380229 1102 80-81 -0.7100882167374429 1102 82-83 -0.41976267451444826 1102 84-85 -0.7153412896431242 1102 86-87 -0.9724140381732127 1102 88-89 -0.35584350155115985 1102 90-91 0.2755050540033466 1102 92-93 -0.8952829863588754 1102 94-95 0.11119690856464715 1102 96-97 -0.18713386469306315 1102 98-99 -0.6228110593589946 1102 100-101 -1.7955949395722257 1103 1 0.3428506646534544 1103 2 0.26139185447063795 1103 3 0.12701621598768043 1103 4 0.1909596248577543 1103 5 0.23677537227594314 1103 6 0.43744942999502 1103 7 0.019858301389653832 1103 8 0.0884912114175691 1103 9 0.32574130307923355 1103 10-11 0.2873980957147282 1103 12-13 0.3444413991517692 1103 14-15 0.17361267415169124 1103 16-17 0.2955529102328569 1103 18-19 0.04535038478656617 1103 20-21 0.09029497596805669 1103 22-23 0.02522614762367681 1103 24-25 -0.03670305629376713 1103 26-27 -0.058491917386184866 1103 28-29 -0.17115293784666363 1103 30-31 -0.04417271043245563 1103 32-33 0.13491766919597836 1103 34-35 -0.15323575961851787 1103 36-37 0.1748683996053657 1103 38-39 0.02634972226524468 1103 40-41 0.40494742415116036 1103 42-43 -0.20269052456659864 1103 44-45 -0.38870911304725553 1103 46-47 -0.26202146802276616 1103 48-49 -0.126017186641036 1103 50-51 -0.344775684951351 1103 52-53 -0.975497600221999 1103 54-55 -0.5778303730537573 1103 56-57 0.04783679104242111 1103 58-59 0.05403726252399821 1103 60-61 0.19540117923729738 1103 62-63 -0.1853377712814286 1103 64-65 0.050175224894161374 1103 66-67 -0.5146629803491898 1103 68-69 -0.3317969909856018 1103 70-71 0.4461801303635511 1103 72-73 -0.18365883635981106 1103 74-75 -0.3256015259609626 1103 76-77 -0.0057232684693282465 1103 78-79 -0.4074230389478828 1103 80-81 0.05300597340340474 1103 82-83 0.9083623254855517 1103 84-85 0.5719606469765957 1103 86-87 -0.2765513621168765 1103 88-89 -0.4276964945089077 1103 90-91 -0.011576812193837327 1103 92-93 -0.05527418354197522 1103 94-95 0.17567754236746325 1103 96-97 0.30087229727876874 1103 98-99 0.15942925754241344 1103 100-101 0.16501245479397042 1104 1 0.4447346961149492 1104 2 0.4497950006202629 1104 3 0.20621870364087158 1104 4 0.1732168042980362 1104 5 -0.027720695037025678 1104 6 0.39135467967491877 1104 7 0.005590878671526411 1104 8 -0.025465254651571456 1104 9 0.31129096468523443 1104 10-11 0.4088541044946794 1104 12-13 0.29971851640071634 1104 14-15 0.4518274171551653 1104 16-17 0.21662479609346974 1104 18-19 0.1310463789332701 1104 20-21 0.389087732507285 1104 22-23 0.2424385127233606 1104 24-25 0.09463039898334813 1104 26-27 0.4649212891256127 1104 28-29 0.5388342580560348 1104 30-31 0.4135737684407772 1104 32-33 0.4493497160223896 1104 34-35 0.3848107009631505 1104 36-37 0.3234873862525234 1104 38-39 0.47988914059339294 1104 40-41 0.7510238829036737 1104 42-43 1.277463124601141 1104 44-45 1.0407768939035407 1104 46-47 1.5192113836326158 1104 48-49 1.9603190123712224 1104 50-51 1.6809239675088676 1104 52-53 1.2179357270141438 1104 54-55 1.542619599508896 1104 56-57 1.0999677586663452 1104 58-59 1.323106221733802 1104 60-61 1.4040165075709332 1104 62-63 1.5196192435347449 1104 64-65 0.15553465419725043 1104 66-67 -0.4264976245782002 1104 68-69 -0.2013781140878521 1104 70-71 0.13366869813381044 1104 72-73 0.84771120205248 1104 74-75 0.7940253260602539 1104 76-77 0.33532300919666724 1104 78-79 0.9253005754658723 1104 80-81 0.016880127418399837 1104 82-83 -0.8318974147741862 1104 84-85 -0.09348658185091452 1104 86-87 -0.38191079141996553 1104 88-89 -0.3638589236290848 1104 90-91 -0.5519097187239268 1104 92-93 -0.7453235707744597 1104 94-95 -0.6308891303963939 1104 96-97 -0.30878565040734784 1104 98-99 -0.02632161130704702 1104 100-101 0.1879683721160852 1105 1 0.5469868743164952 1105 2 0.712987504319095 1105 3 0.6518869594357071 1105 4 0.32823899729491046 1105 5 0.5006370231941162 1105 6 0.5041279823216414 1105 7 0.44435464654922185 1105 8 0.28638744960619533 1105 9 0.39135014601023954 1105 10-11 0.4874337526945851 1105 12-13 0.5268268511039906 1105 14-15 0.6997314118946036 1105 16-17 0.6634437998745 1105 18-19 0.684145178867503 1105 20-21 0.5102450561962613 1105 22-23 0.4952742845464755 1105 24-25 0.4736374678638313 1105 26-27 0.43607039318610674 1105 28-29 0.6544844306682265 1105 30-31 0.5085104773015345 1105 32-33 1.0435466583206008 1105 34-35 1.140934324175383 1105 36-37 1.2329892767670714 1105 38-39 1.2550001055777713 1105 40-41 1.413772526664978 1105 42-43 0.9529521924952675 1105 44-45 0.8234499170828968 1105 46-47 1.2173866261116615 1105 48-49 1.6296433589107622 1105 50-51 1.9206905300602415 1105 52-53 2.1682891710384737 1105 54-55 2.477437945719643 1105 56-57 2.318116698334272 1105 58-59 2.901603673648971 1105 60-61 2.8926734202784807 1105 62-63 2.66962746316322 1105 64-65 2.604819546520119 1105 66-67 2.6716447393869522 1105 68-69 2.327143996712742 1105 70-71 2.5970091551451517 1105 72-73 2.6820034920409697 1105 74-75 2.5323945517712545 1105 76-77 2.5197714721261413 1105 78-79 3.2950194641721033 1105 80-81 2.710698966806863 1105 82-83 3.1425395406754255 1105 84-85 2.964543995167002 1105 86-87 3.39032330359894 1105 88-89 2.855687352879219 1105 90-91 3.100564389446383 1105 92-93 2.756813532628467 1105 94-95 3.009337731349458 1105 96-97 2.679460280876558 1105 98-99 2.8800568203878427 1105 100-101 3.0534952503011894 1106 1 0.29851054989080694 1106 2 0.07429395845255016 1106 3 0.3965345606233939 1106 4 0.01881564989687945 1106 5 0.12635979237679607 1106 6 0.38911001076358076 1106 7 0.3182411913218388 1106 8 0.2618523659993812 1106 9 0.4551101085043783 1106 10-11 0.2534909491315247 1106 12-13 0.2365471198959881 1106 14-15 0.36131915998024056 1106 16-17 0.18079026025893086 1106 18-19 0.172893420780305 1106 20-21 0.7056662157524372 1106 22-23 0.48253791948943814 1106 24-25 0.7660269037131897 1106 26-27 0.809878800749793 1106 28-29 0.640966693521797 1106 30-31 0.7715984597988026 1106 32-33 1.6948202948263074 1106 34-35 1.112804928957381 1106 36-37 0.9926392220710198 1106 38-39 1.3807228747604583 1106 40-41 1.1055212374010281 1106 42-43 0.7740961212341304 1106 44-45 0.40697859343858056 1106 46-47 0.34404704180160905 1106 48-49 0.2659146513001929 1106 50-51 -0.07360545368722171 1106 52-53 -0.07643656735814375 1106 54-55 -0.5906977004750757 1106 56-57 -0.4683343096357291 1106 58-59 -0.7645160325551217 1106 60-61 -0.7230477861600271 1106 62-63 -0.6198708959553976 1106 64-65 -0.4927738274445659 1106 66-67 -0.5930841244980307 1106 68-69 -1.2198164658595374 1106 70-71 -0.8884573239922133 1106 72-73 -0.8705828495749053 1106 74-75 -0.12989643119483674 1106 76-77 -0.12587628533595918 1106 78-79 -0.2609267774281463 1106 80-81 -1.3519670747621326 1106 82-83 -0.9311438473539546 1106 84-85 -0.9309084192727539 1106 86-87 -0.5916270011361746 1106 88-89 -0.5220279151314067 1106 90-91 -0.7977009645151725 1106 92-93 -0.14856230734653053 1106 94-95 0.2722694394288432 1106 96-97 -0.06628124123627188 1106 98-99 0.1799474282953284 1106 100-101 -0.31507796426358325 1107 1 0.25642300780326366 1107 2 0.18680798763325157 1107 3 0.19647844390060953 1107 4 0.2625025185837515 1107 5 -0.13486355217987978 1107 6 0.027718316038551905 1107 7 -0.08369483561418889 1107 8 -0.2527379819242981 1107 9 0.06453771793199081 1107 10-11 0.1320683902089641 1107 12-13 0.05458864627084381 1107 14-15 -0.05710115427340412 1107 16-17 -0.17379728182861243 1107 18-19 0.11481261269950238 1107 20-21 -0.12065252723296993 1107 22-23 -0.03191213662728387 1107 24-25 -0.07095401660107115 1107 26-27 0.18326544496976993 1107 28-29 -0.06343846921669893 1107 30-31 0.24880104116805057 1107 32-33 0.29512893680161056 1107 34-35 0.1437392723917199 1107 36-37 0.4419938797590177 1107 38-39 0.5115449847492357 1107 40-41 0.7875498569296511 1107 42-43 0.9820085791465942 1107 44-45 1.1154522185788665 1107 46-47 1.040315279736511 1107 48-49 1.2963579734101813 1107 50-51 1.5892031882880886 1107 52-53 2.029624038702458 1107 54-55 1.7577170021062969 1107 56-57 2.069123602822188 1107 58-59 1.577164663292244 1107 60-61 1.5355100140644389 1107 62-63 1.4165348279503256 1107 64-65 1.5556969918595875 1107 66-67 1.069443933863358 1107 68-69 1.2434270807173462 1107 70-71 -0.07574688628177029 1107 72-73 -0.8170615252202467 1107 74-75 -0.5687993492644239 1107 76-77 -0.45282634145268474 1107 78-79 0.5916966793619771 1107 80-81 0.4170424650807405 1107 82-83 0.10154414366737186 1107 84-85 -0.09105151691585078 1107 86-87 0.6838359618267873 1107 88-89 -0.5838264560966131 1107 90-91 -0.0519097187239268 1107 92-93 -0.138180713631602 1107 94-95 -0.722609909617173 1107 96-97 -0.6480713646930631 1107 98-99 -0.002782650268088105 1107 100-101 -0.3794017577540423 1108 1 0.25543486551077166 1108 2 0.3241597662893767 1108 3 0.20141915536306243 1108 4 0.296593427674658 1108 5 0.2119743924841515 1108 6 0.2698131776986301 1108 7 0.021542318536006633 1108 8 0.06445569396503004 1108 9 0.10406340963159266 1108 10-11 0.05079368665165873 1108 12-13 0.19984556326689074 1108 14-15 -0.12553000802834902 1108 16-17 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0.07229675171506145 2316 84-85 0.5183933415044173 2316 86-87 0.9246146503513799 2316 88-89 0.30412063779310117 2316 90-91 0.6224568982656393 2316 92-93 0.7243379152804685 2316 94-95 0.6416784659416983 2316 96-97 0.12446961891349417 2316 98-99 0.21415103080493836 2316 100-101 -7.691199000952054E-4 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 9.0 14 52.0 15 167.0 16 443.0 17 898.0 18 1297.0 19 1809.0 20 2448.0 21 3131.0 22 3167.0 23 2336.0 24 2218.0 25 2634.0 26 3327.0 27 3783.0 28 4696.0 29 4559.0 30 1982.0 31 1083.0 32 1113.0 33 1378.0 34 1840.0 35 2899.0 36 4261.0 37 10073.0 38 20667.0 39 7517.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.701303480035236 23.1459696932529 15.71981311954328 25.43291370716858 2 12.600512306492927 21.392137209043323 47.220180420982295 18.787170063481458 3 13.181868804989419 33.69417529791736 40.0545717785945 13.06938411849872 4 8.75932731930059 28.629023276534138 33.16293573894643 29.448713665218847 5 7.461855440472213 39.3540483350039 39.43646285777926 13.747633366744626 6 18.459739391914468 44.51943423543824 18.253703084976056 18.767123287671232 7 15.568548836173294 38.167947432898984 21.29078962022497 24.97271411070275 8 21.49237108809444 38.12562646174407 23.44024947098786 16.94175297917363 9 22.27085421539147 14.65976166610981 18.66354827931841 44.405835839180305 10-11 18.543267624457066 30.501169395255594 29.221516872702974 21.734046107584362 12-13 22.892861120392023 26.924490477781486 28.680253925826932 21.50239447599955 14-15 21.01124846864907 27.89787281434458 24.863013698630137 26.22786501837621 16-17 19.25158703641831 34.975498385120844 25.833611760775142 19.93930281768571 18-19 19.53948101124847 26.41496825927163 30.772357723577237 23.273193005902662 20-21 17.873371199465417 25.480008909678137 32.0982292014701 24.548390689386345 22-23 16.311393250918808 32.59383004788952 30.687158926383784 20.407617774807886 24-25 19.736050785165386 33.895199910903216 30.547388350595835 15.821360953335562 26-27 19.792849983294353 33.25314623009244 32.18454170843078 14.769462078182425 28-29 13.74262167279207 26.58258157924045 35.48335003897984 24.191446708987637 30-31 19.584029401937855 26.715113041541372 38.60841964584029 15.092437910680475 32-33 14.36796970709433 33.522663993763224 34.75387014144114 17.355496157701303 34-35 12.504733266510748 33.73092772023611 39.45483906893863 14.30949994431451 36-37 18.86123176300256 31.355941641608197 32.24189776144337 17.540928833945873 38-39 14.82069272747522 27.237999777258047 37.11994654193117 20.82136095333556 40-41 13.298808330549058 26.735716672235217 39.829602405613095 20.135872591602627 42-43 18.18855106359283 25.798529903107248 39.2705201024613 16.742398930838622 44-45 13.443033745405947 30.07350484463749 38.23198574451498 18.251475665441586 46-47 19.371634452073483 25.198104434309514 32.974712797295865 22.455548316321135 48-49 17.816671214340207 25.076428758373755 33.69603350057635 23.410866526709693 50-51 14.320637041986858 26.315848089987746 36.34703196347032 23.016482904555073 52-53 13.674685376990755 27.857222407840514 40.95333556075287 17.51475665441586 54-55 13.910791847644505 28.34224301147121 34.38411849871923 23.362846642165053 56-57 18.65519545606415 30.66989642499165 28.072168392916808 22.602739726027394 58-59 12.457957456286891 32.93406838177971 36.04243234213164 18.56554181980176 60-61 17.55596391580354 37.90121394364628 26.249582358837287 18.293239781712884 62-63 12.254148568882949 42.45294576233434 26.552511415525114 18.7403942532576 64-65 12.712440138100012 41.6933957010803 26.424434792293127 19.16972936852656 66-67 13.121171622675131 41.18331662768682 25.726695623120616 19.96881612651743 68-69 13.305304190449673 39.657524711123486 26.528191563413618 20.508979535013225 70-71 14.054460407617775 37.40282882280878 27.33266510747299 21.21004566210046 72-73 14.1869918699187 35.502839959906446 27.510858670230537 22.799309499944314 74-75 14.467646731261832 34.175297917362734 27.55874819022163 23.798307161153804 76-77 14.63080521216171 33.44971600400935 28.69417529791736 23.22530348591157 78-79 15.086312506960686 32.43289898652411 28.164049448713662 24.316739057801538 80-81 15.081857667891748 31.896647733600624 27.67067602182871 25.35081857667892 82-83 15.586368192449049 31.3336674462635 27.87949660318521 25.200467758102242 84-85 15.481679474328992 30.814121839848536 27.612206259048893 26.09199242677358 86-87 15.085198797193453 31.696179975498385 27.434012696291347 25.78460853101682 88-89 16.109811783049338 30.09744960463303 27.17451832052567 26.61822029179196 90-91 16.153803318855108 29.465419311727363 27.086535248914135 27.294242120503398 92-93 16.046887181200578 29.115714444815683 27.33099454282214 27.506403831161595 94-95 15.91491257378327 28.487025281211714 27.532576010691614 28.0654861343134 96-97 16.5970598062145 28.43579463191892 27.01414411404388 27.953001447822697 98-99 16.077116699708192 29.059096072884415 27.03261087474662 27.83117635266077 100-101 16.024612985855885 28.87570998997661 27.54371310836396 27.555963915803545 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 426.0 1 257.5 2 89.0 3 86.0 4 70.0 5 61.5 6 94.0 7 149.5 8 220.5 9 248.5 10 192.0 11 144.5 12 141.5 13 135.0 14 125.0 15 121.5 16 130.0 17 141.0 18 160.5 19 199.5 20 230.5 21 262.0 22 305.0 23 358.0 24 424.0 25 530.0 26 708.5 27 918.0 28 1109.0 29 1357.0 30 1749.0 31 2150.0 32 2664.0 33 3204.5 34 3634.0 35 4354.5 36 5332.0 37 5857.5 38 5452.5 39 4889.0 40 4518.0 41 4150.0 42 3875.5 43 3623.5 44 3491.0 45 3410.5 46 2947.0 47 2324.0 48 2028.0 49 1921.5 50 2149.0 51 2152.0 52 1494.5 53 835.5 54 497.0 55 419.5 56 367.0 57 238.5 58 159.5 59 120.5 60 112.0 61 109.5 62 90.0 63 69.5 64 50.5 65 37.0 66 29.0 67 28.5 68 23.0 69 16.5 70 9.0 71 3.5 72 3.5 73 3.5 74 2.5 75 2.0 76 2.5 77 2.0 78 1.5 79 2.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11916694509410848 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.001670564650851988 48-49 5.568548836173293E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0027842744180866467 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.004454839068938635 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 89790.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.653079407506404 #Duplication Level Percentage of deduplicated Percentage of total 1 73.8132733408324 25.578572224078407 2 10.654025389683433 7.383895756765786 3 4.762976056564358 4.951553625125292 4 2.5903904869034227 3.5906002895645397 5 1.8961915474851359 3.2854438133422432 6 1.1473565804274466 2.385566321416639 7 0.7874015748031495 1.9100122508074395 8 0.6781295195243451 1.8799420870921038 9 0.5174353205849269 1.6137654527230205 >10 2.9021372328458943 18.818353936964026 >50 0.16712196689699502 4.058358391803097 >100 0.05784991161819058 4.6475108586702305 >500 0.0064277679575767315 1.5692170620336339 >1k 0.016069419893941828 9.484352377770353 >5k 0.0032138839787883657 8.84285555184319 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 7940 8.84285555184319 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 2529 2.8165720013364517 TruSeq Adapter, Index 11 (95% over 21bp) ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 1741 1.9389687047555406 RNA PCR Primer, Index 11 (95% over 23bp) TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 1519 1.6917251364294463 TruSeq Adapter, Index 11 (96% over 28bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1429 1.591491257378327 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1298 1.445595277870587 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 755 0.8408508742621673 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 654 0.7283661877714668 TruSeq Adapter, Index 11 (96% over 26bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 486 0.5412629468760441 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 474 0.5278984296692282 No Hit CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA 469 0.5223298808330549 RNA PCR Primer, Index 11 (96% over 28bp) CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 379 0.4220960017819356 TruSeq Adapter, Index 11 (95% over 24bp) CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC 296 0.32965809110145894 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 281 0.3129524445929391 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 204 0.22719679251587038 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 200 0.22274195344693173 No Hit TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT 191 0.2127185655418198 No Hit ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGC 190 0.21160485577458513 RNA PCR Primer, Index 17 (95% over 24bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 170 0.18933066042989197 No Hit CTTTACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 134 0.14923710880944424 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 133 0.1481233990422096 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGT 119 0.13253146230092439 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 118 0.13141775253368973 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 112 0.12473549393028176 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 109 0.1213943646285778 No Hit CTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 108 0.12028065486134315 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 100 0.11137097672346587 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 95 0.10580242788729256 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 95 0.10580242788729256 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 92 0.10246129858558858 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 90 0.10023387905111927 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0011137097672346587 2 0.0 0.0 0.0 0.0 0.0011137097672346587 3 0.0 0.0 0.0 0.0 0.0011137097672346587 4 0.0 0.0 0.0 0.0 0.0011137097672346587 5 0.0 0.0 0.0 0.0 0.0011137097672346587 6 0.0 0.0 0.0 0.0 0.0011137097672346587 7 0.0 0.0 0.0 0.0 0.0011137097672346587 8 0.0 0.0 0.0 0.0011137097672346587 0.0011137097672346587 9 0.0 0.0 0.0 0.0011137097672346587 0.0011137097672346587 10-11 0.0 0.0 0.0 0.0011137097672346587 0.0022274195344693173 12-13 0.0 0.0 0.0 0.0011137097672346587 0.0022274195344693173 14-15 0.0 0.0 0.0 0.0022274195344693173 0.0027842744180866467 16-17 0.0 0.0 0.0 0.0027842744180866467 0.003341129301703976 18-19 0.0 0.0 0.0 0.004454839068938635 0.003341129301703976 20-21 0.0 0.0 0.0 0.00835282325425994 0.003341129301703976 22-23 0.0 0.0 0.0 0.029513308831718453 0.004454839068938635 24-25 0.0 0.0 0.0 0.07740282882280877 0.004454839068938635 26-27 0.0 0.0 0.0 0.1425548502060363 0.005568548836173293 28-29 0.0 0.0 0.0 0.2890076845973939 0.005568548836173293 30-31 0.0 0.0 0.0 0.5028399599064484 0.005568548836173293 32-33 0.0 0.0 0.0 0.7940750640383116 0.005568548836173293 34-35 0.0 0.0 0.0 1.3609533355607528 0.005568548836173293 36-37 0.0 0.0 0.0 2.1299699298362844 0.005568548836173293 38-39 0.0 0.0 0.0 2.90455507294799 0.006125403719790623 40-41 0.0 0.0 0.0 3.5622006905000556 0.006682258603407952 42-43 0.0 0.0 0.0 4.062256375988417 0.006682258603407952 44-45 0.0 0.0 0.0 4.522775364739949 0.007239113487025281 46-47 0.0 0.0 0.0 5.108029847421761 0.00835282325425994 48-49 0.0 0.0 0.0 5.616438356164384 0.00890967813787727 50-51 0.0 0.0 0.0 6.05524000445484 0.00890967813787727 52-53 0.0 0.0 0.0 6.441140438801648 0.014478226974050563 54-55 0.0 0.0 0.0 6.9105691056910565 0.01614879162490255 56-57 0.0 0.0 0.0 7.553179641385455 0.01726250139213721 58-59 0.0 0.0 0.0 8.040427664550618 0.01781935627575454 60-61 0.0 0.0 0.0 8.464194230983406 0.018376211159371866 62-63 0.0 0.0 0.0 8.866243456955118 0.020046775810223856 64-65 0.0 0.0 0.0 9.30170397594387 0.020603630693841184 66-67 0.0 0.0 0.0 9.810669339570108 0.021717340461075846 68-69 0.0 0.0 0.0 10.376990756208933 0.022274195344693173 70-71 0.0 0.0 0.0 10.918253703084975 0.022274195344693173 72-73 0.0 0.0 0.0 11.415525114155251 0.0228310502283105 74-75 0.0 0.0 0.0 11.945650963358949 0.023387905111927832 76-77 0.0 0.0 0.0 12.568214723243123 0.023387905111927832 78-79 0.0 0.0 0.0 13.169061142666221 0.023387905111927832 80-81 0.0 0.0 0.0 13.803318855106358 0.023387905111927832 82-83 0.0 0.0 0.0 14.4119612429001 0.023387905111927832 84-85 0.0 0.0 0.0 15.035081857667892 0.023387905111927832 86-87 0.0 0.0 0.0 15.668782715224413 0.02450161487916249 88-89 0.0 0.0 0.0 16.347588818353934 0.02561532464639715 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGAGTG 20 0.0020678327 71.25 7 CATGAGG 25 0.0050062453 57.0 6 CTATACA 25 0.0050062453 57.0 1 GGATATG 25 0.0050062453 57.0 1 TGGGAGT 25 0.0050062453 57.0 6 GTACCCA 50 2.8060489E-5 47.5 1 ACCCATG 50 2.8060489E-5 47.5 3 TACCCAT 55 4.9266695E-5 43.18182 2 GGGGCAG 70 4.265852E-6 40.714287 7 TGGGGCA 70 4.265852E-6 40.714287 6 TGCTTAT 25 0.0016121852 38.0 12-13 ATGGGGC 80 1.0665901E-5 35.625 5 ATCTTTA 30 0.0039421534 31.666668 86-87 CTTATAC 2285 0.0 30.765863 1 GGTATCA 1220 0.0 30.758196 1 TATACAC 2330 0.0 30.171673 3 TTATACA 2335 0.0 30.107069 2 GTATCAA 1725 0.0 29.463768 1 AACGCAG 1695 0.0 29.144543 6 GGGCAGT 100 4.903099E-5 28.5 8 >>END_MODULE