FastQCFastQC Report
Fri 10 Feb 2017
SRR2031517_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031517_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471854
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.14763692594510053No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC18650.1267109373619938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17370.11801442262615722No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC17240.1171311828482988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20100.054.353231
GTATCAA39200.039.9872471
TCAACGC43550.035.4477584
AACGCAG44650.034.680856
CAACGCA44700.034.5357935
ATCAACG45100.034.018853
TATCAAC47700.032.264152
ACGCAGA49800.030.8082357
CGCAGAG49950.030.4304318
GTATACG650.005844597729.230771
GTACATG57750.028.294371
ACATGGG54000.027.6203693
GCAGAGT54500.027.5412859
CATGGGG21250.025.7058854
TACATGG62300.025.084272
CGCCGTA6300.023.37301494-95
CATGGGA30750.023.3252034
CAGAGTA54200.023.00507410-11
GCCGTAT4900.022.2959294-95
GAGTACT27050.022.03789312-13
Produced by FastQC (version 0.11.5)