FastQCFastQC Report
Fri 10 Feb 2017
SRR2031517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1471854
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT82270.5589548963416209No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.18480093813652712No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT24020.16319553433968315TruSeq Adapter, Index 11 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.14063894924360706No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC19570.13296155732837633No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC18950.12874918300320548No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19550.053.0465741
GTATCAA38900.040.1117741
TCAACGC42950.036.0526854
CAACGCA43450.035.5284925
ATCAACG43750.035.393443
AACGCAG44150.034.9651876
TATCAAC47700.032.8608472
ACGCAGA49950.030.9051657
CGCAGAG50250.030.8151828
ACATGGG46850.028.8947163
GTACATG52300.028.6521031
GCAGAGT55650.027.1422069
CATGGGG16200.026.9746634
TACATGG55550.025.9939772
GCTTCGT1858.974894E-925.6750588
CATGGGA28450.022.8729154
CAGAGTA55650.022.23441710-11
GAGTACT31100.022.06940712-13
AGAGTAC52250.021.72675310-11
ATGGGAT15550.021.6875825
Produced by FastQC (version 0.11.5)