FastQCFastQC Report
Fri 10 Feb 2017
SRR2031516_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031516_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097705
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32250.15373944382074695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.11197952047594872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23750.053.01
GTATCAA41750.042.7784421
TCAACGC48500.035.94334
ATCAACG49700.035.075453
CAACGCA50300.034.657065
AACGCAG51700.033.7185676
TATCAAC55650.031.5813142
ACGCAGA60750.028.7736637
GTACATG60650.028.194561
CGCAGAG63200.027.583078
TACATGG61950.026.6061332
ACATGGG61800.025.9021033
CATGGGG45950.023.362354
GCAGAGT76850.021.8802859
GAGTACT40350.021.13073212-13
AGAGTAC64600.020.47794210-11
CAGAGTA69950.020.47355310-11
GTACTTT43500.019.014-15
AGTACTT44200.017.83936712-13
CATGGGA22750.016.9120884
Produced by FastQC (version 0.11.5)