##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031516_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2097705 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.213408463058435 34.0 31.0 34.0 30.0 34.0 2 32.3711103324824 34.0 31.0 34.0 30.0 34.0 3 32.47995166145859 34.0 31.0 34.0 30.0 34.0 4 35.7072543565468 37.0 35.0 37.0 35.0 37.0 5 35.68428020145826 37.0 35.0 37.0 35.0 37.0 6 35.80527719579254 37.0 37.0 37.0 35.0 37.0 7 35.83154781058347 37.0 37.0 37.0 35.0 37.0 8 35.88314324464117 37.0 37.0 37.0 35.0 37.0 9 37.717539882871996 39.0 39.0 39.0 35.0 39.0 10-11 37.7205560362396 39.0 39.0 39.0 35.0 39.0 12-13 37.68961746289397 39.0 39.0 39.0 35.0 39.0 14-15 39.21696735241609 41.0 40.0 41.0 36.0 41.0 16-17 39.221488007131605 41.0 40.0 41.0 36.0 41.0 18-19 39.16863548497048 41.0 39.5 41.0 36.0 41.0 20-21 39.1289642728601 41.0 39.5 41.0 36.0 41.0 22-23 39.059370836223394 41.0 39.0 41.0 36.0 41.0 24-25 39.00761951752034 41.0 39.0 41.0 36.0 41.0 26-27 38.90375505612086 41.0 39.0 41.0 35.0 41.0 28-29 38.80211111667275 41.0 39.0 41.0 35.0 41.0 30-31 38.65267709234616 40.5 39.0 41.0 35.0 41.0 32-33 38.558303002567094 40.0 38.0 41.0 34.5 41.0 34-35 38.43564895921972 40.0 38.0 41.0 34.0 41.0 36-37 38.30766432839698 40.0 38.0 41.0 34.0 41.0 38-39 38.16573088208304 40.0 38.0 41.0 33.5 41.0 40-41 38.04235581266193 40.0 38.0 41.0 33.0 41.0 42-43 37.89945178182823 40.0 38.0 41.0 33.0 41.0 44-45 38.09345451338487 40.0 38.0 41.0 33.0 41.0 46-47 37.98886759577729 40.0 38.0 41.0 33.0 41.0 48-49 37.877611484932345 40.0 38.0 41.0 33.0 41.0 50-51 36.81887276809656 39.0 36.5 40.0 31.5 40.5 52-53 36.8771841131141 39.5 37.0 40.0 31.5 40.5 54-55 37.65174583652134 40.0 37.0 41.0 33.0 41.0 56-57 37.67868742268336 40.0 37.0 41.0 33.0 41.0 58-59 37.47271232132259 40.0 37.0 41.0 32.0 41.0 60-61 37.29511561444531 40.0 36.0 41.0 32.0 41.0 62-63 37.02761994656065 39.0 36.0 41.0 31.5 41.0 64-65 36.733764995554665 39.0 35.0 41.0 31.0 41.0 66-67 36.43334668125404 39.0 35.0 41.0 31.0 41.0 68-69 36.12104013672084 37.5 35.0 40.5 31.0 41.0 70-71 35.7505800386613 37.0 35.0 40.0 30.5 41.0 72-73 35.38303145580527 36.5 35.0 39.0 30.0 41.0 74-75 35.00357128385545 36.0 35.0 39.0 30.0 41.0 76-77 34.635137209474166 35.5 35.0 38.0 29.5 40.0 78-79 34.24298578684801 35.0 34.0 37.0 29.0 39.0 80-81 33.917201179384136 35.0 34.0 37.0 29.0 39.0 82-83 33.62921907513211 35.0 34.0 36.0 29.0 38.0 84-85 33.34998343427698 35.0 34.0 36.0 29.0 37.0 86-87 33.1031145942828 35.0 34.0 35.5 28.0 37.0 88-89 32.907286296214195 35.0 34.0 35.0 27.0 36.5 90-91 32.73472080201935 35.0 34.0 35.0 27.0 36.0 92-93 32.59083331545665 35.0 34.0 35.0 27.0 36.0 94-95 32.47026321622917 35.0 34.0 35.0 26.0 36.0 96-97 32.350357414412414 35.0 33.5 35.0 25.5 35.5 98-99 32.22329927229997 35.0 33.0 35.0 25.0 35.0 100-101 30.63712604965903 34.0 30.0 35.0 21.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18325068908717412 1101 2 0.18187587532236904 1101 3 0.3063673921019259 1101 4 0.17679504174380156 1101 5 0.20140263206074138 1101 6 0.15435160741181164 1101 7 0.1242426706191253 1101 8 0.21898591291216718 1101 9 0.4698059428826227 1101 10-11 0.5335425501225473 1101 12-13 0.6126307597571028 1101 14-15 0.6935967378265389 1101 16-17 0.677497988963502 1101 18-19 0.7341134500918329 1101 20-21 0.6495022671872164 1101 22-23 0.6731881657501688 1101 24-25 0.7707824036910012 1101 26-27 0.6820650874172074 1101 28-29 0.6851124138372526 1101 30-31 0.8603817075988331 1101 32-33 0.7647945701644687 1101 34-35 0.6909432182348567 1101 36-37 0.770569250427279 1101 38-39 0.8924689572129765 1101 40-41 0.7496035203497939 1101 42-43 0.7937140099005973 1101 44-45 0.8352766350980119 1101 46-47 0.7501803572454264 1101 48-49 0.7523657305146543 1101 50-51 0.8832226808136383 1101 52-53 0.903499795691161 1101 54-55 0.8273773482607965 1101 56-57 0.7670833076774883 1101 58-59 0.7510385759700355 1101 60-61 0.8155584495659198 1101 62-63 0.7977855707140336 1101 64-65 0.7908529342708377 1101 66-67 0.7510788347863198 1101 68-69 0.7767658851858883 1101 70-71 0.7206809621606567 1101 72-73 0.8432652777439813 1101 74-75 0.8448715033594283 1101 76-77 0.7749037639903662 1101 78-79 0.7181550436267727 1101 80-81 0.8466515154110894 1101 82-83 0.7821751380343471 1101 84-85 0.7051023718934388 1101 86-87 0.7040726503220966 1101 88-89 0.7848450695472522 1101 90-91 0.6697811784968195 1101 92-93 0.5546637237743184 1101 94-95 0.5336233698054471 1101 96-97 0.6005486092154797 1101 98-99 0.7333193939056812 1101 100-101 0.589265586649077 1102 1 0.17309997986731673 1102 2 0.2217293876492903 1102 3 0.35683387302053404 1102 4 0.1883916307340101 1102 5 0.2632820646848586 1102 6 0.1663281356117423 1102 7 0.3031307151986624 1102 8 0.34626554817052835 1102 9 0.478500218465193 1102 10-11 0.40066936538766385 1102 12-13 0.519772561513264 1102 14-15 0.5550255203324497 1102 16-17 0.49858039355654427 1102 18-19 0.5152515791023049 1102 20-21 0.6292852796829962 1102 22-23 0.569883750012238 1102 24-25 0.5671497626913435 1102 26-27 0.7058735558400357 1102 28-29 0.6986329727700422 1102 30-31 0.7202052469436495 1102 32-33 0.6154130267669728 1102 34-35 0.6142028855769723 1102 36-37 0.701410815776832 1102 38-39 0.7989015391109788 1102 40-41 0.7134069229164979 1102 42-43 0.6653758217884729 1102 44-45 0.7264947624198612 1102 46-47 0.6452253167185731 1102 48-49 0.6732455008625067 1102 50-51 0.5804723430898946 1102 52-53 0.6199722712061941 1102 54-55 0.5588711425870372 1102 56-57 0.4779358490486558 1102 58-59 0.588415095726873 1102 60-61 0.5406418251147258 1102 62-63 0.5985570583871151 1102 64-65 0.6459430642944781 1102 66-67 0.5657223251611967 1102 68-69 0.5909819439498207 1102 70-71 0.587949199242729 1102 72-73 0.5011310747720188 1102 74-75 0.5524185736059621 1102 76-77 0.49867212771883374 1102 78-79 0.48093894264737713 1102 80-81 0.43292832223310285 1102 82-83 0.4842827452616305 1102 84-85 0.5503489102250896 1102 86-87 0.5107785773737703 1102 88-89 0.5465577510737631 1102 90-91 0.418341631046637 1102 92-93 0.4612999953042092 1102 94-95 0.3853109331286575 1102 96-97 0.4453951559902194 1102 98-99 0.4460389143379686 1102 100-101 0.2882397507828145 1103 1 0.21022266384589727 1103 2 0.25235502652257225 1103 3 0.3478747541410243 1103 4 0.2949062765866657 1103 5 0.18950582433988217 1103 6 0.16739602710990908 1103 7 0.21300473446471102 1103 8 0.21313958774513253 1103 9 0.30590932075938326 1103 10-11 0.406132874299729 1103 12-13 0.46049561375364334 1103 14-15 0.5028990454236606 1103 16-17 0.5253883626333788 1103 18-19 0.5241159247268286 1103 20-21 0.5266521063359377 1103 22-23 0.42418188636387555 1103 24-25 0.4019550713475226 1103 26-27 0.4214295702184998 1103 28-29 0.38139659473306864 1103 30-31 0.46299554080843563 1103 32-33 0.44338433754877826 1103 34-35 0.4409671912613362 1103 36-37 0.5285801078883097 1103 38-39 0.5126426456564346 1103 40-41 0.5584743134703061 1103 42-43 0.5136881808978728 1103 44-45 0.6043614841452722 1103 46-47 0.598052944476315 1103 48-49 0.6375241071540927 1103 50-51 0.6411925521326225 1103 52-53 0.6542483727760384 1103 54-55 0.658805985974233 1103 56-57 0.5966203962694223 1103 58-59 0.7145616828742689 1103 60-61 0.6811169437505313 1103 62-63 0.7086329785338776 1103 64-65 0.6935062069756199 1103 66-67 0.6802385415420744 1103 68-69 0.623652485037411 1103 70-71 0.6420757283076597 1103 72-73 0.601163229983527 1103 74-75 0.5527771888333177 1103 76-77 0.578845393537506 1103 78-79 0.556735376465177 1103 80-81 0.6021119521841598 1103 82-83 0.508478188021968 1103 84-85 0.4865116765210118 1103 86-87 0.5780286636851244 1103 88-89 0.7128223647712133 1103 90-91 0.6195244351164675 1103 92-93 0.6483348447839674 1103 94-95 0.6811850810155491 1103 96-97 0.5741295607126347 1103 98-99 0.6344731924703879 1103 100-101 0.6321107982303715 1104 1 0.03763594961537109 1104 2 0.10726696502020872 1104 3 0.16675134404101755 1104 4 0.02252725682826906 1104 5 0.04413996253897068 1104 6 -0.0310189612296341 1104 7 0.0235556613400405 1104 8 0.022786769443214894 1104 9 0.13607557171916795 1104 10-11 0.20033710163337304 1104 12-13 0.24873369218632035 1104 14-15 0.3424896417047165 1104 16-17 0.26246111054332033 1104 18-19 0.32359120407708275 1104 20-21 0.3196634615721834 1104 22-23 0.2783325566614181 1104 24-25 0.3323649042858179 1104 26-27 0.3289995687400733 1104 28-29 0.28056283615464395 1104 30-31 0.20299115323290806 1104 32-33 0.19643119876309356 1104 34-35 0.1176124128577456 1104 36-37 0.21337572794334392 1104 38-39 0.2827831673010124 1104 40-41 0.31293700238643396 1104 42-43 0.20270684240118442 1104 44-45 0.2054861580577949 1104 46-47 0.3468099967886147 1104 48-49 0.3946807436005386 1104 50-51 0.3150555977777145 1104 52-53 0.341746286293116 1104 54-55 0.3697064881608725 1104 56-57 0.22469766052069673 1104 58-59 0.36557952886082745 1104 60-61 0.3795402780217856 1104 62-63 0.4577196951489597 1104 64-65 0.4479408774067011 1104 66-67 0.4013175035943206 1104 68-69 0.3754587831159384 1104 70-71 0.3159566580921336 1104 72-73 0.31816341597857445 1104 74-75 0.4441614450677349 1104 76-77 0.3577753735549578 1104 78-79 0.26404116663415067 1104 80-81 0.44516968695521797 1104 82-83 0.29141779209096796 1104 84-85 0.38738883394436385 1104 86-87 0.357913656746085 1104 88-89 0.3212461521073351 1104 90-91 0.3804015686942961 1104 92-93 0.2356237523253455 1104 94-95 0.27339629985541336 1104 96-97 0.3060498880639244 1104 98-99 0.14845441650857794 1104 100-101 0.1619904260495062 1105 1 0.21332989018910098 1105 2 0.1751667384995983 1105 3 0.2811320844802552 1105 4 0.24815638242332483 1105 5 0.22850318302493378 1105 6 0.18067162577728624 1105 7 0.2514809626301471 1105 8 0.11568012778820247 1105 9 0.2944099373729756 1105 10-11 0.25055930861658027 1105 12-13 0.3074166872134825 1105 14-15 0.39304864324435584 1105 16-17 0.31330492192952164 1105 18-19 0.33937630592287604 1105 20-21 0.3823133323846477 1105 22-23 0.31946628788056586 1105 24-25 0.36056660938429275 1105 26-27 0.3476499129452151 1105 28-29 0.3050647783918876 1105 30-31 0.34565489400838345 1105 32-33 0.2109952129560213 1105 34-35 0.18431156075092758 1105 36-37 0.29094631654298553 1105 38-39 0.3482225155233536 1105 40-41 0.22338393816429658 1105 42-43 0.2381211494781894 1105 44-45 0.30569387568570505 1105 46-47 0.2865353847936305 1105 48-49 0.3637752805605601 1105 50-51 0.32799426024431 1105 52-53 0.2290622382990648 1105 54-55 0.1739768661671306 1105 56-57 0.2819449927096329 1105 58-59 0.31046522886259 1105 60-61 0.15964993257785665 1105 62-63 0.29372507943762827 1105 64-65 0.26347977081812957 1105 66-67 0.22856678244472306 1105 68-69 0.1951370973071036 1105 70-71 0.17352187125156604 1105 72-73 0.20617563127933636 1105 74-75 0.2651050892180109 1105 76-77 0.20387242790221194 1105 78-79 0.21159799587654504 1105 80-81 0.3151116852917042 1105 82-83 0.33868627210229363 1105 84-85 0.3376035197355023 1105 86-87 0.2668309147849044 1105 88-89 0.2612110015950009 1105 90-91 0.32028622900187287 1105 92-93 0.33620642349882957 1105 94-95 0.17691710258782223 1105 96-97 0.31394105641474823 1105 98-99 0.32936507801954207 1105 100-101 0.28926558664907986 1106 1 0.11572249359845443 1106 2 0.11201894132069867 1106 3 0.08256308967920489 1106 4 0.0628330534397108 1106 5 0.05848283256199949 1106 6 0.05862270181448537 1106 7 0.041316605456223954 1106 8 -0.04235493921815703 1106 9 0.06042311080241802 1106 10-11 0.08384539055696649 1106 12-13 0.1575425183201773 1106 14-15 0.19128105278058172 1106 16-17 0.16707610091003033 1106 18-19 0.15370409336669155 1106 20-21 0.22777085532423058 1106 22-23 0.18902202122217915 1106 24-25 0.2578072574921748 1106 26-27 0.2448966454790309 1106 28-29 0.2582927605373371 1106 30-31 0.19228438094886968 1106 32-33 0.17344640809263012 1106 34-35 0.24423008122566614 1106 36-37 0.23041417523931074 1106 38-39 0.3596864801870794 1106 40-41 0.20460299821110794 1106 42-43 0.17861636996742902 1106 44-45 0.3596787863093738 1106 46-47 0.3002351818068263 1106 48-49 0.2181343186516571 1106 50-51 0.14005747613526864 1106 52-53 0.12503279485574126 1106 54-55 0.23325924049137114 1106 56-57 0.2335802792731485 1106 58-59 0.3007331248422176 1106 60-61 0.3019280193236469 1106 62-63 0.2360870535878803 1106 64-65 0.2157772241622382 1106 66-67 0.23213303278131292 1106 68-69 0.20034044658940076 1106 70-71 0.2763670523862203 1106 72-73 0.2424951231909347 1106 74-75 0.20306646994255573 1106 76-77 0.19124931536881462 1106 78-79 0.1057829676033819 1106 80-81 0.07096490304683556 1106 82-83 0.1418895281763639 1106 84-85 0.23816419311315684 1106 86-87 0.2492950813997865 1106 88-89 0.11417359920473302 1106 90-91 0.1924524399838674 1106 92-93 0.06180658091717106 1106 94-95 0.027240224442046213 1106 96-97 0.0821092817300979 1106 98-99 0.15530143233508653 1106 100-101 0.03221671446862828 1107 1 0.05562299765120571 1107 2 0.09510876885212838 1107 3 0.11052225422485407 1107 4 0.08335229715365955 1107 5 0.06820285870798415 1107 6 0.08255772149092167 1107 7 -0.0015971383496307112 1107 8 0.024049050357525914 1107 9 0.17986644960109288 1107 10-11 0.09575654289142221 1107 12-13 0.1492283867065396 1107 14-15 0.24715142321858252 1107 16-17 0.19557985178794723 1107 18-19 0.11078767166649328 1107 20-21 0.20261934127928072 1107 22-23 0.14042128187989533 1107 24-25 0.17206302805018936 1107 26-27 0.2328038832188426 1107 28-29 0.2155021998714588 1107 30-31 0.1910930831317259 1107 32-33 0.05561771786292269 1107 34-35 0.16623141773839478 1107 36-37 0.21748067587652997 1107 38-39 0.09197738228447605 1107 40-41 0.3013338978253728 1107 42-43 0.25155880375953643 1107 44-45 0.2103422346339201 1107 46-47 0.24891406697506824 1107 48-49 0.26926578987463756 1107 50-51 0.28723588841171477 1107 52-53 0.24903025815946478 1107 54-55 0.2097479367341606 1107 56-57 0.21467130211923546 1107 58-59 0.27111658714050435 1107 60-61 0.18448433726761237 1107 62-63 0.25859195596037665 1107 64-65 0.3193034024053176 1107 66-67 0.3211952950421093 1107 68-69 0.3325686616145802 1107 70-71 0.4394248912524503 1107 72-73 0.22257950120737036 1107 74-75 0.2150614890590674 1107 76-77 0.3052790472994147 1107 78-79 0.3004150066616873 1107 80-81 0.27141795408142144 1107 82-83 0.2275786173392902 1107 84-85 0.2703673333094443 1107 86-87 0.3399243987436549 1107 88-89 0.2560227582850416 1107 90-91 0.35550577511870074 1107 92-93 0.46555088582709203 1107 94-95 0.35194891213992463 1107 96-97 0.2957362340063696 1107 98-99 0.3687242560286066 1107 100-101 0.5171371262314004 1108 1 0.019818799938072118 1108 2 0.03296063982926967 1108 3 -0.03878125478852468 1108 4 -0.02335783361398569 1108 5 0.044357354933495685 1108 6 0.04969053533270795 1108 7 0.02610644891733216 1108 8 0.04574235319319797 1108 9 0.11058951040799059 1108 10-11 0.025732896206335454 1108 12-13 0.15358590547658935 1108 14-15 0.21889517004095183 1108 16-17 0.1516522954948485 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-0.8183285979994537 2316 56-57 -0.7209944609192362 2316 58-59 -0.724662626747552 2316 60-61 -0.8105079549307561 2316 62-63 -0.7240111353291425 2316 64-65 -0.9813125439598664 2316 66-67 -0.7811791680291975 2316 68-69 -0.7731736728995529 2316 70-71 -1.0492625743799238 2316 72-73 -0.8726384691446611 2316 74-75 -0.6893548146313435 2316 76-77 -0.7240492137302823 2316 78-79 -0.7548023001933615 2316 80-81 -0.5700244598204804 2316 82-83 -0.6571382469169009 2316 84-85 -0.5443885898725824 2316 86-87 -0.5528246137249084 2316 88-89 -0.5628538022036764 2316 90-91 -0.43387236946906427 2316 92-93 -0.5016038732387855 2316 94-95 -0.5726109459002657 2316 96-97 -0.5398807834070354 2316 98-99 -0.42260799418751915 2316 100-101 -0.3816167122859184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 37.0 7 775.0 8 1658.0 9 1338.0 10 1368.0 11 1828.0 12 2295.0 13 2582.0 14 2979.0 15 3188.0 16 3559.0 17 4255.0 18 5349.0 19 6432.0 20 8170.0 21 10231.0 22 11735.0 23 12154.0 24 11717.0 25 11736.0 26 12169.0 27 13417.0 28 15630.0 29 18626.0 30 22379.0 31 28208.0 32 35969.0 33 48585.0 34 71535.0 35 115293.0 36 220826.0 37 478675.0 38 690032.0 39 222783.0 40 192.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.220090527505064 19.26734216679657 13.575979463270574 23.936587842427794 2 14.899950183653088 21.012439785384505 40.46660517088914 23.62100486007327 3 17.504987593584417 26.764201830095267 30.8067626286823 24.924047947638016 4 12.219592364035934 17.980125899494926 39.548315897611914 30.251965838857224 5 13.14036053687244 38.22348709661273 35.6246469355796 13.011505430935236 6 29.611647014236986 38.289845330968845 19.050104757341952 13.048402897452217 7 26.246493191368664 33.232127491711175 23.579387950164584 16.941991366755573 8 25.30913545994313 35.329657888025245 21.395429767293304 17.96577688473832 9 26.49948395985136 16.02255798598945 20.53382148586193 36.94413656829726 10-11 23.8279453021278 26.38037760314248 29.87541146157348 19.916265633156236 12-13 25.151129448611698 24.142408012566115 29.1602966098665 21.546165928955695 14-15 22.495608295732715 25.403047616323555 26.658419558517522 25.442924529426207 16-17 22.15006399851266 28.444466690978953 27.123832950772393 22.281636359735998 18-19 22.189726391461146 27.868194050164348 28.518809842184673 21.423269716189836 20-21 23.032695255052545 27.135774572687772 28.24451007172124 21.587020100538446 22-23 22.890015516957817 26.943111638671784 27.929999690137553 22.23687315423284 24-25 22.5323389132409 27.059071699786198 28.016618161276252 22.391971225696654 26-27 22.46004562128612 27.289275660781666 27.954335809849333 22.29634290808288 28-29 22.450702076793448 27.253712986335067 28.110840180101587 22.1847447567699 30-31 22.82256084625817 27.121163366631627 27.945421305665004 22.110854481445198 32-33 22.648942534817813 27.236765894155756 27.833322607325623 22.280968963700808 34-35 22.784352423243497 27.285295120143203 27.77189833651538 22.158454120097918 36-37 22.819080852646106 27.11510913116954 27.776498602043663 22.28931141414069 38-39 22.649300068408092 27.33606488996308 27.743104964711435 22.271530076917394 40-41 22.689749035255197 27.199796920920722 27.758764935965736 22.35168910785835 42-43 22.634402835479726 27.223179617725084 27.724775409316372 22.417642137478815 44-45 22.78020503359624 27.237337947900205 27.571178978931737 22.411278039571815 46-47 22.67709332194944 27.201952804988128 27.49246138807709 22.628492484985344 48-49 22.646520768604624 27.283152122744102 27.527086245752784 22.543240862898486 50-51 22.662576482393852 27.397679845354805 27.34452651826639 22.59521715398495 52-53 22.782874617737004 27.332942429941294 27.360567858683655 22.523615093638046 54-55 22.676424950124062 27.255834352304063 27.409573796124814 22.658166901447057 56-57 22.712225980297514 27.315041914854564 27.36097306341931 22.61175904142861 58-59 22.645557883496487 27.16776191123156 27.487230091933807 22.69945011333815 60-61 22.630374623695896 27.349984864411347 27.467732593477155 22.5519079184156 62-63 22.575862668964415 27.340212279610338 27.4458038666066 22.638121184818647 64-65 22.558176674031856 27.08147713811046 27.527988921225816 22.832357266631867 66-67 22.512102512031003 27.181944076979363 27.405903117931263 22.900050293058367 68-69 22.58060594792881 27.166808488324147 27.42752198235691 22.825063581390136 70-71 22.557437771278614 26.892175019843112 27.51066045988354 23.039726748994735 72-73 22.555602432181836 27.011901101441815 27.378444538197698 23.05405192817865 74-75 22.488004748046077 27.153031527311988 27.330964077408403 23.027999647233525 76-77 22.488123925909505 27.059500740094535 27.337566531042256 23.114808802953704 78-79 22.451321801683267 27.104907506060194 27.279526911553344 23.1642437807032 80-81 22.493034053882695 27.199010347022103 27.157917819712495 23.150037779382707 82-83 22.65273239087479 27.092894377426756 27.178773945812207 23.07559928588624 84-85 22.68760383371351 27.035903523136 27.079927825885907 23.19656481726458 86-87 22.603797960151688 27.06400566333207 27.23659904514696 23.095597331369284 88-89 22.642054054311735 26.957675173582558 27.226635775764468 23.17363499634124 90-91 22.690750129307983 26.880948465108297 27.21612428821021 23.21217711737351 92-93 22.760397672694683 26.858256999911806 27.1929799471327 23.18836538026081 94-95 22.719686514548044 26.817355157183687 27.178964630393693 23.283993697874582 96-97 22.7404711339297 26.744704331638623 27.219604281822278 23.2952202526094 98-99 22.771719569720243 26.727280528005604 27.10724339218336 23.39375651009079 100-101 23.12298440438479 26.651268886711904 27.021983548687732 23.20376316021557 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 53.0 1 45.5 2 61.0 3 84.5 4 75.5 5 85.5 6 125.5 7 240.5 8 466.0 9 611.5 10 577.0 11 566.0 12 636.5 13 726.5 14 840.0 15 940.5 16 1031.0 17 1140.5 18 1298.5 19 1484.0 20 1687.5 21 2037.5 22 2515.5 23 3103.5 24 3977.0 25 5260.0 26 7137.0 27 9778.5 28 12957.5 29 17199.5 30 22302.0 31 27770.5 32 33463.5 33 39090.5 34 44633.5 35 49060.5 36 54201.0 37 59490.0 38 62936.5 39 66099.0 40 68387.0 41 70234.5 42 72225.5 43 74995.5 44 77958.5 45 81282.5 46 84110.0 47 85431.5 48 86429.0 49 86295.5 50 84375.0 51 81643.0 52 77940.0 53 73505.0 54 69629.5 55 64722.5 56 58290.5 57 50944.0 58 44356.0 59 38397.0 60 32000.0 61 25767.5 62 20174.0 63 15201.0 64 10855.5 65 7648.0 66 5451.5 67 3834.0 68 2686.5 69 1893.0 70 1340.0 71 988.5 72 765.0 73 543.5 74 397.0 75 310.0 76 215.5 77 145.5 78 110.5 79 88.0 80 76.5 81 65.5 82 46.0 83 37.0 84 31.5 85 22.5 86 18.5 87 17.0 88 14.0 89 12.5 90 10.5 91 4.5 92 3.5 93 4.5 94 3.0 95 2.5 96 2.5 97 1.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 2.1452015416848413E-4 48-49 3.5753359028080687E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2097705.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.7327496290359 #Duplication Level Percentage of deduplicated Percentage of total 1 67.70600724090234 35.703239282161945 2 16.134975404619592 17.016832365649144 3 6.664763885300712 10.54353975900609 4 3.3269007437697327 7.017464958474505 5 1.9332487010835342 5.097275986244844 6 1.1613036248144049 3.6743239974377917 7 0.7288920741154568 2.6905538275643344 8 0.5107778067211732 2.154777455831657 9 0.3676616554255728 1.7449029021378237 >10 1.418260247885601 11.892623940864047 >50 0.03655200021829904 1.2917181482117992 >100 0.010383664850470114 0.8425143556959508 >500 0.0 0.0 >1k 2.7295029316381136E-4 0.3302330207199891 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3225 0.15373944382074695 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2349 0.11197952047594872 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 4.7671145370774254E-5 4.7671145370774254E-5 8 0.0 0.0 0.0 9.534229074154851E-5 4.7671145370774254E-5 9 0.0 0.0 0.0 1.4301343611232276E-4 9.534229074154851E-5 10-11 0.0 0.0 0.0 1.4301343611232276E-4 9.534229074154851E-5 12-13 0.0 0.0 0.0 1.4301343611232276E-4 9.534229074154851E-5 14-15 0.0 0.0 0.0 1.9068458148309702E-4 9.534229074154851E-5 16-17 0.0 0.0 0.0 2.8602687222464547E-4 9.534229074154851E-5 18-19 0.0 0.0 0.0 4.2904030833696826E-4 1.4301343611232276E-4 20-21 0.0 0.0 0.0 5.720537444492909E-4 1.4301343611232276E-4 22-23 0.0 0.0 0.0 8.342450439885494E-4 1.9068458148309702E-4 24-25 0.0 0.0 0.0 0.0015254766518647761 2.860268722246455E-4 26-27 0.0 0.0 0.0 0.002121365968999454 3.3369801759541975E-4 28-29 0.0 0.0 0.0 0.005863550880605233 3.3369801759541975E-4 30-31 0.0 0.0 0.0 0.015779149117726276 3.3369801759541975E-4 32-33 0.0 0.0 0.0 0.03889965462255179 3.8136916296619403E-4 34-35 0.0 0.0 0.0 0.06955220109595964 3.8136916296619403E-4 36-37 0.0 0.0 0.0 0.11622225241394762 3.8136916296619403E-4 38-39 0.0 0.0 0.0 0.1753583082463931 4.2904030833696826E-4 40-41 0.0 0.0 0.0 0.23482806209643395 4.2904030833696826E-4 42-43 0.0 0.0 0.0 0.3231626944684787 4.2904030833696826E-4 44-45 0.0 0.0 0.0 0.41206938058497267 4.2904030833696826E-4 46-47 0.0 0.0 0.0 0.5301508076683804 4.2904030833696826E-4 48-49 0.0 0.0 0.0 0.6920658529202152 4.2904030833696826E-4 50-51 0.0 0.0 0.0 0.846782555221063 4.2904030833696826E-4 52-53 0.0 0.0 0.0 1.0025241871473825 5.482181717639039E-4 54-55 0.0 0.0 0.0 1.1795986566271235 6.912316078762266E-4 56-57 0.0 0.0 0.0 1.3765043225811064 7.389027532470008E-4 58-59 0.0 0.0 0.0 1.5953148798329604 7.865738986177752E-4 60-61 0.0 0.0 0.0 1.8313108849909783 9.057517620447107E-4 62-63 0.0 0.0 0.0 2.076841119223151 0.0010487651981570334 64-65 0.0 0.0 0.0 2.339985841669825 0.0010487651981570334 66-67 0.0 0.0 0.0 2.655711837460463 0.0010487651981570334 68-69 0.0 0.0 0.0 3.0136744680496066 0.0010487651981570334 70-71 0.0 0.0 0.0 3.378906948307794 0.001144107488898582 72-73 0.0 0.0 0.0 3.741231488698363 0.0012871209250109048 74-75 0.0 0.0 0.0 4.107870267744988 0.0012871209250109048 76-77 0.0 0.0 0.0 4.4899306623190585 0.0012871209250109048 78-79 0.0 0.0 0.0 4.911748792132354 0.0012871209250109048 80-81 0.0 0.0 0.0 5.358308246393082 0.001358627643067066 82-83 0.0 0.0 0.0 5.817095349441413 0.0014301343611232275 84-85 0.0 0.0 0.0 6.280697238172193 0.0014301343611232275 86-87 0.0 0.0 0.0 6.7882757585075115 0.0014301343611232275 88-89 0.0 0.0 0.0 7.349555824103009 0.0014301343611232275 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2375 0.0 53.0 1 GTATCAA 4175 0.0 42.778442 1 TCAACGC 4850 0.0 35.9433 4 ATCAACG 4970 0.0 35.07545 3 CAACGCA 5030 0.0 34.65706 5 AACGCAG 5170 0.0 33.718567 6 TATCAAC 5565 0.0 31.581314 2 ACGCAGA 6075 0.0 28.773663 7 GTACATG 6065 0.0 28.19456 1 CGCAGAG 6320 0.0 27.58307 8 TACATGG 6195 0.0 26.606133 2 ACATGGG 6180 0.0 25.902103 3 CATGGGG 4595 0.0 23.36235 4 GCAGAGT 7685 0.0 21.880285 9 GAGTACT 4035 0.0 21.130732 12-13 AGAGTAC 6460 0.0 20.477942 10-11 CAGAGTA 6995 0.0 20.473553 10-11 GTACTTT 4350 0.0 19.0 14-15 AGTACTT 4420 0.0 17.839367 12-13 CATGGGA 2275 0.0 16.912088 4 >>END_MODULE