FastQCFastQC Report
Fri 10 Feb 2017
SRR2031516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2097705
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42970.20484291165821697No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT40630.19368786364145577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32140.15321506122166845No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24000.053.695641
GTATCAA44200.044.1105541
TCAACGC51750.036.8059354
ATCAACG51800.036.7704053
CAACGCA53050.035.9039965
AACGCAG55000.034.8037576
TATCAAC56200.033.97612
ACGCAGA62800.030.2540957
CGCAGAG64650.029.3148848
GTACATG57500.024.8931371
TACATGG57650.024.3056092
ACATGGG56350.023.8548283
GCAGAGT79000.023.5089639
GAGTACT47000.021.67770212-13
CATGGGG39100.020.7731884
AGAGTAC66750.020.529510-11
CAGAGTA73100.020.37060710-11
TATACCG1656.326997E-520.151065
GAGTACA42150.019.29211
GTACTTT51950.019.1092914-15
Produced by FastQC (version 0.11.5)